Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-09_19.56.35 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "b67e255aeeee8af2705a2d7c6fcdb620",
"name": "M1_20170523_1540_dsRNA_G2_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "17:57:00"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8792 (max 8793) in '8792'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.0178
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 7.17486e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 50 frames Cell Events: 5 mean_circ: 0.948293
Ran for 550 frames Cell Events: 15 mean_circ: 0.931867
Ran for 1550 frames Cell Events: 90 mean_circ: 0.941257
Ran for 3550 frames Cell Events: 193 mean_circ: 0.939426
Ran for 4050 frames Cell Events: 226 mean_circ: 0.939208
Ran for 5550 frames Cell Events: 323 mean_circ: 0.939101
Ran for 6050 frames Cell Events: 365 mean_circ: 0.938835
Ran for 7050 frames Cell Events: 405 mean_circ: 0.940352
Ran for 7550 frames Cell Events: 434 mean_circ: 0.940311
Ran for 8550 frames Cell Events: 495 mean_circ: 0.938938
Ran for 11050 frames Cell Events: 658 mean_circ: 0.938957
Ran for 12050 frames Cell Events: 726 mean_circ: 0.937907
Ran for 12550 frames Cell Events: 748 mean_circ: 0.937727
Ran for 13050 frames Cell Events: 776 mean_circ: 0.93831
Ran for 13550 frames Cell Events: 801 mean_circ: 0.93842
Ran for 15550 frames Cell Events: 941 mean_circ: 0.939033
Ran for 16050 frames Cell Events: 970 mean_circ: 0.938364
Ran for 17050 frames Cell Events: 1014 mean_circ: 0.938051
Ran for 18550 frames Cell Events: 1093 mean_circ: 0.938609
Ran for 19050 frames Cell Events: 1118 mean_circ: 0.938519
Ran for 19550 frames Cell Events: 1148 mean_circ: 0.938466
Ran for 20550 frames Cell Events: 1211 mean_circ: 0.937667
Ran for 21550 frames Cell Events: 1286 mean_circ: 0.937667
Ran for 22050 frames Cell Events: 1319 mean_circ: 0.938036
Ran for 25050 frames Cell Events: 1496 mean_circ: 0.937815
Ran for 26050 frames Cell Events: 1556 mean_circ: 0.937854
Ran for 26550 frames Cell Events: 1584 mean_circ: 0.937559
Ran for 27050 frames Cell Events: 1626 mean_circ: 0.937687
Ran for 27550 frames Cell Events: 1673 mean_circ: 0.93817
Ran for 28050 frames Cell Events: 1702 mean_circ: 0.937845
Ran for 29050 frames Cell Events: 1769 mean_circ: 0.937752
Ran for 30050 frames Cell Events: 1822 mean_circ: 0.93773
Ran for 30550 frames Cell Events: 1843 mean_circ: 0.937681
Ran for 31550 frames Cell Events: 1900 mean_circ: 0.937502
Ran for 32050 frames Cell Events: 1930 mean_circ: 0.937406
Ran for 32550 frames Cell Events: 1960 mean_circ: 0.937299
Ran for 33050 frames Cell Events: 1983 mean_circ: 0.937279
Ran for 36550 frames Cell Events: 2174 mean_circ: 0.937014
Ran for 38550 frames Cell Events: 2316 mean_circ: 0.937265
Ran for 39050 frames Cell Events: 2340 mean_circ: 0.937247
Ran for 40050 frames Cell Events: 2407 mean_circ: 0.937034
Ran for 41050 frames Cell Events: 2446 mean_circ: 0.936942
Ran for 41550 frames Cell Events: 2479 mean_circ: 0.937083
Ran for 43050 frames Cell Events: 2565 mean_circ: 0.937092
Ran for 43550 frames Cell Events: 2592 mean_circ: 0.937137
Ran for 44050 frames Cell Events: 2612 mean_circ: 0.937169
Ran for 44550 frames Cell Events: 2640 mean_circ: 0.937147
Ran for 47550 frames Cell Events: 2826 mean_circ: 0.937089
Ran for 48050 frames Cell Events: 2855 mean_circ: 0.937101
Ran for 49050 frames Cell Events: 2908 mean_circ: 0.937044
Ran for 53550 frames Cell Events: 3169 mean_circ: 0.936954
Ran for 54050 frames Cell Events: 3204 mean_circ: 0.937099
Ran for 55550 frames Cell Events: 3279 mean_circ: 0.937201
Ran for 56050 frames Cell Events: 3324 mean_circ: 0.937237
Ran for 57050 frames Cell Events: 3373 mean_circ: 0.937293
Ran for 57550 frames Cell Events: 3399 mean_circ: 0.937327
Ran for 58050 frames Cell Events: 3428 mean_circ: 0.93726
Ran for 59550 frames Cell Events: 3531 mean_circ: 0.937163
Ran for 60050 frames Cell Events: 3551 mean_circ: 0.93711
Ran for 61050 frames Cell Events: 3603 mean_circ: 0.937094
Ran for 61550 frames Cell Events: 3621 mean_circ: 0.93706
Ran for 62550 frames Cell Events: 3669 mean_circ: 0.93708
Ran for 63050 frames Cell Events: 3699 mean_circ: 0.937088
Ran for 63550 frames Cell Events: 3718 mean_circ: 0.937142
Ran for 64050 frames Cell Events: 3741 mean_circ: 0.937181
Ran for 65050 frames Cell Events: 3813 mean_circ: 0.936955
Ran for 66050 frames Cell Events: 3866 mean_circ: 0.937048
Ran for 67550 frames Cell Events: 3953 mean_circ: 0.937126
Ran for 68050 frames Cell Events: 3972 mean_circ: 0.937182
Ran for 68550 frames Cell Events: 4001 mean_circ: 0.937204
Ran for 69050 frames Cell Events: 4014 mean_circ: 0.937105
Ran for 69550 frames Cell Events: 4034 mean_circ: 0.937109
Ran for 73050 frames Cell Events: 4254 mean_circ: 0.937213
Ran for 74550 frames Cell Events: 4365 mean_circ: 0.937171
Ran for 75050 frames Cell Events: 4401 mean_circ: 0.93719
Ran for 75550 frames Cell Events: 4433 mean_circ: 0.937172
Ran for 76050 frames Cell Events: 4467 mean_circ: 0.937103
Ran for 76550 frames Cell Events: 4482 mean_circ: 0.937101
Ran for 77050 frames Cell Events: 4506 mean_circ: 0.93711
Ran for 77550 frames Cell Events: 4522 mean_circ: 0.937133
Ran for 78050 frames Cell Events: 4544 mean_circ: 0.9371
Ran for 79550 frames Cell Events: 4649 mean_circ: 0.937298
Ran for 81550 frames Cell Events: 4777 mean_circ: 0.937299
Ran for 82550 frames Cell Events: 4832 mean_circ: 0.937301
Ran for 83550 frames Cell Events: 4898 mean_circ: 0.937206
Ran for 84050 frames Cell Events: 4916 mean_circ: 0.937217
Ran for 84550 frames Cell Events: 4936 mean_circ: 0.937201
Ran for 86050 frames Cell Events: 5033 mean_circ: 0.93712
Ran for 86550 frames Cell Events: 5064 mean_circ: 0.937116
Ran for 87050 frames Cell Events: 5096 mean_circ: 0.937144
Ran for 88050 frames Cell Events: 5156 mean_circ: 0.937126
Ran for 88550 frames Cell Events: 5191 mean_circ: 0.937128
Ran for 90550 frames Cell Events: 5322 mean_circ: 0.937064
Ran for 91050 frames Cell Events: 5352 mean_circ: 0.937074
Ran for 91550 frames Cell Events: 5394 mean_circ: 0.93709
Ran for 92050 frames Cell Events: 5423 mean_circ: 0.937037
Ran for 93050 frames Cell Events: 5492 mean_circ: 0.937105
Ran for 95550 frames Cell Events: 5652 mean_circ: 0.936972
Ran for 97550 frames Cell Events: 5791 mean_circ: 0.936955
Ran for 98550 frames Cell Events: 5852 mean_circ: 0.936905
Ran for 99050 frames Cell Events: 5898 mean_circ: 0.936905
Ran for 99550 frames Cell Events: 5940 mean_circ: 0.936903
Ran for 100550 frames Cell Events: 6007 mean_circ: 0.9369
Ran for 101550 frames Cell Events: 6081 mean_circ: 0.936864
Ran for 102550 frames Cell Events: 6139 mean_circ: 0.936835
Ran for 103050 frames Cell Events: 6152 mean_circ: 0.936835
Ran for 104050 frames Cell Events: 6187 mean_circ: 0.936779
Ran for 105050 frames Cell Events: 6247 mean_circ: 0.936838
Ran for 105550 frames Cell Events: 6269 mean_circ: 0.936862
Ran for 106550 frames Cell Events: 6318 mean_circ: 0.936965
Ran for 107050 frames Cell Events: 6343 mean_circ: 0.936976
Ran for 107550 frames Cell Events: 6362 mean_circ: 0.936961
Ran for 108050 frames Cell Events: 6385 mean_circ: 0.936965
Ran for 108550 frames Cell Events: 6411 mean_circ: 0.93698
Ran for 109050 frames Cell Events: 6438 mean_circ: 0.937011
Ran for 109550 frames Cell Events: 6476 mean_circ: 0.937026
Ran for 110550 frames Cell Events: 6535 mean_circ: 0.936955
Ran for 111050 frames Cell Events: 6574 mean_circ: 0.936959
Ran for 111550 frames Cell Events: 6603 mean_circ: 0.936872
Ran for 112550 frames Cell Events: 6646 mean_circ: 0.936773
Ran for 113550 frames Cell Events: 6698 mean_circ: 0.936802
Ran for 116050 frames Cell Events: 6816 mean_circ: 0.936793
Ran for 117050 frames Cell Events: 6870 mean_circ: 0.936792
Ran for 117550 frames Cell Events: 6899 mean_circ: 0.936823
Ran for 118050 frames Cell Events: 6924 mean_circ: 0.936837
Ran for 121050 frames Cell Events: 7064 mean_circ: 0.936953
Ran for 121550 frames Cell Events: 7094 mean_circ: 0.936983
Ran for 123050 frames Cell Events: 7181 mean_circ: 0.937054
Ran for 123550 frames Cell Events: 7209 mean_circ: 0.937044
Ran for 125050 frames Cell Events: 7318 mean_circ: 0.937063
Ran for 125550 frames Cell Events: 7347 mean_circ: 0.937063
Ran for 126050 frames Cell Events: 7384 mean_circ: 0.93699
Ran for 126550 frames Cell Events: 7408 mean_circ: 0.937014
Ran for 127050 frames Cell Events: 7447 mean_circ: 0.937014
Ran for 130050 frames Cell Events: 7612 mean_circ: 0.937007
Ran for 131050 frames Cell Events: 7693 mean_circ: 0.937094
Ran for 133050 frames Cell Events: 7834 mean_circ: 0.937015
Ran for 133550 frames Cell Events: 7856 mean_circ: 0.93699
Ran for 135050 frames Cell Events: 7968 mean_circ: 0.937043
Ran for 135550 frames Cell Events: 7993 mean_circ: 0.937026
Ran for 136050 frames Cell Events: 8026 mean_circ: 0.937031
Ran for 137050 frames Cell Events: 8092 mean_circ: 0.937052
Ran for 137550 frames Cell Events: 8129 mean_circ: 0.937071
Ran for 138050 frames Cell Events: 8153 mean_circ: 0.937083
Ran for 140050 frames Cell Events: 8278 mean_circ: 0.937107
Ran for 141050 frames Cell Events: 8328 mean_circ: 0.937116
Ran for 142050 frames Cell Events: 8381 mean_circ: 0.937132
Ran for 142550 frames Cell Events: 8394 mean_circ: 0.937147
Ran for 143050 frames Cell Events: 8414 mean_circ: 0.93716
Ran for 143550 frames Cell Events: 8440 mean_circ: 0.937167
Ran for 144550 frames Cell Events: 8495 mean_circ: 0.937171
Ran for 146550 frames Cell Events: 8626 mean_circ: 0.937107



End this measurment..



Stopping time: 7.17561e+008
****************************************
Measurement finished

Mean Circularitiy: 0.937147
Mean Area: 599.462
Mean FL-1 intensity: 1798.49
Mean FL-2 intensity: 2691.05
Mean FL-3 intensity: 0

frames processed: 145812
Total Time: 74801.8ms
Number of Runs (new bg img) 0
Frames collected: 149452
Skipped frames: 57102
Rescued Frames: 2028064
Samples DAQ: 37363043
Samples written: 74725746
Samples per Channel: 37362873
FPS: 1997.97

Samples per second: 499492
Expected samples/s: 500000

Events: 8793
Events per second: 117.551
jumped back (PMT): 17
#Frames read, frequency
# 0 frames :228856
# 1 frames :57390
# 2 frames :2852
# 3 frames :953
# 4 frames :494
# 5 frames :228
# 6 frames :2514
# 7 frames :1107
# 8 frames :1062
# 9 frames :590
# 10 frames :408
# 11 frames :268
# 12 frames :214
# 13 frames :159
# 14 frames :272
# 15 frames :216
# 16 frames :165
# 17 frames :96
# 18 frames :82
# 19 frames :65
# 20 frames :63
# 21 frames :69
# 22 frames :48
# 23 frames :52
# 24 frames :31
# 25 frames :33
# 26 frames :30
# 27 frames :34
# 28 frames :27
# 29 frames :20
# 30 frames :12
# 31 frames :23
# 32 frames :14
# 33 frames :13
# 34 frames :12
# 35 frames :8
# 36 frames :6
# 37 frames :9
# 38 frames :4
# 39 frames :8
# 40 frames :2
# 41 frames :5
# 42 frames :4
# 43 frames :3
# 44 frames :2
# 45 frames :2
# 46 frames :1
# 47 frames :2
# 48 frames :2
# 49 frames :1
# 50 frames :3
# 51 frames :1
# 52 frames :1
# 54 frames :3
# 55 frames :1
# 56 frames :2
# 59 frames :1
# 64 frames :2
# 65 frames :1
# 82 frames :1
# 94 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.0178
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0