Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-09_19.57.42 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "576510ca72c675461992a79d748eb785",
"name": "M1_20170523_1552_dsRNA_G6_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "17:58:06"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 9476 (max 9477) in '9476'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.1295
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 7.1821e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 39 mean_circ: 0.93421
Ran for 1550 frames Cell Events: 71 mean_circ: 0.940068
Ran for 2050 frames Cell Events: 91 mean_circ: 0.936877
Ran for 3550 frames Cell Events: 179 mean_circ: 0.935884
Ran for 5050 frames Cell Events: 273 mean_circ: 0.937457
Ran for 5550 frames Cell Events: 298 mean_circ: 0.937058
Ran for 6050 frames Cell Events: 323 mean_circ: 0.938025
Ran for 7550 frames Cell Events: 392 mean_circ: 0.939006
Ran for 8550 frames Cell Events: 410 mean_circ: 0.938835
Ran for 11050 frames Cell Events: 593 mean_circ: 0.936585
Ran for 12550 frames Cell Events: 674 mean_circ: 0.936772
Ran for 14050 frames Cell Events: 735 mean_circ: 0.936961
Ran for 14550 frames Cell Events: 768 mean_circ: 0.937052
Ran for 15050 frames Cell Events: 795 mean_circ: 0.937189
Ran for 16050 frames Cell Events: 867 mean_circ: 0.937025
Ran for 16550 frames Cell Events: 894 mean_circ: 0.937178
Ran for 18550 frames Cell Events: 999 mean_circ: 0.936939
Ran for 19550 frames Cell Events: 1050 mean_circ: 0.936806
Ran for 20050 frames Cell Events: 1072 mean_circ: 0.93694
Ran for 20550 frames Cell Events: 1093 mean_circ: 0.936817
Ran for 21050 frames Cell Events: 1108 mean_circ: 0.936742
Ran for 21550 frames Cell Events: 1135 mean_circ: 0.93672
Ran for 22550 frames Cell Events: 1179 mean_circ: 0.936615
Ran for 23550 frames Cell Events: 1250 mean_circ: 0.936467
Ran for 24550 frames Cell Events: 1295 mean_circ: 0.936623
Ran for 26050 frames Cell Events: 1372 mean_circ: 0.936357
Ran for 26550 frames Cell Events: 1401 mean_circ: 0.936226
Ran for 27050 frames Cell Events: 1428 mean_circ: 0.936309
Ran for 28550 frames Cell Events: 1496 mean_circ: 0.936401
Ran for 29550 frames Cell Events: 1576 mean_circ: 0.936305
Ran for 30050 frames Cell Events: 1594 mean_circ: 0.936025
Ran for 31050 frames Cell Events: 1643 mean_circ: 0.936169
Ran for 31550 frames Cell Events: 1672 mean_circ: 0.936147
Ran for 32050 frames Cell Events: 1695 mean_circ: 0.93618
Ran for 32550 frames Cell Events: 1716 mean_circ: 0.936084
Ran for 35550 frames Cell Events: 1887 mean_circ: 0.936106
Ran for 38550 frames Cell Events: 2029 mean_circ: 0.936279
Ran for 39550 frames Cell Events: 2084 mean_circ: 0.93635
Ran for 40050 frames Cell Events: 2108 mean_circ: 0.936352
Ran for 40550 frames Cell Events: 2142 mean_circ: 0.93641
Ran for 41050 frames Cell Events: 2164 mean_circ: 0.936485
Ran for 41550 frames Cell Events: 2193 mean_circ: 0.936362
Ran for 42050 frames Cell Events: 2216 mean_circ: 0.936341
Ran for 42550 frames Cell Events: 2239 mean_circ: 0.936357
Ran for 43550 frames Cell Events: 2302 mean_circ: 0.936367
Ran for 44050 frames Cell Events: 2337 mean_circ: 0.936373
Ran for 45050 frames Cell Events: 2405 mean_circ: 0.936452
Ran for 45550 frames Cell Events: 2440 mean_circ: 0.936397
Ran for 46050 frames Cell Events: 2460 mean_circ: 0.93643
Ran for 46550 frames Cell Events: 2482 mean_circ: 0.936365
Ran for 48050 frames Cell Events: 2558 mean_circ: 0.936394
Ran for 48550 frames Cell Events: 2575 mean_circ: 0.936531
Ran for 49550 frames Cell Events: 2626 mean_circ: 0.936483
Ran for 50550 frames Cell Events: 2672 mean_circ: 0.936362
Ran for 51550 frames Cell Events: 2717 mean_circ: 0.936417
Ran for 52050 frames Cell Events: 2768 mean_circ: 0.936375
Ran for 52550 frames Cell Events: 2799 mean_circ: 0.936347
Ran for 54050 frames Cell Events: 2867 mean_circ: 0.936589
Ran for 54550 frames Cell Events: 2896 mean_circ: 0.936577
Ran for 55050 frames Cell Events: 2927 mean_circ: 0.936626
Ran for 55550 frames Cell Events: 2959 mean_circ: 0.936594
Ran for 56050 frames Cell Events: 2980 mean_circ: 0.936549
Ran for 56550 frames Cell Events: 3004 mean_circ: 0.936498
Ran for 57050 frames Cell Events: 3026 mean_circ: 0.936512
Ran for 58550 frames Cell Events: 3096 mean_circ: 0.936524
Ran for 60050 frames Cell Events: 3197 mean_circ: 0.936442
Ran for 60550 frames Cell Events: 3222 mean_circ: 0.936577
Ran for 61050 frames Cell Events: 3255 mean_circ: 0.936679
Ran for 62050 frames Cell Events: 3314 mean_circ: 0.936283
Ran for 63050 frames Cell Events: 3354 mean_circ: 0.93629
Ran for 63550 frames Cell Events: 3379 mean_circ: 0.936232
Ran for 64550 frames Cell Events: 3438 mean_circ: 0.936155
Ran for 65050 frames Cell Events: 3466 mean_circ: 0.936142
Ran for 66550 frames Cell Events: 3544 mean_circ: 0.935929
Ran for 67550 frames Cell Events: 3602 mean_circ: 0.935882
Ran for 68050 frames Cell Events: 3627 mean_circ: 0.935902
Ran for 71050 frames Cell Events: 3775 mean_circ: 0.935907
Ran for 71550 frames Cell Events: 3798 mean_circ: 0.935876
Ran for 72550 frames Cell Events: 3859 mean_circ: 0.935917
Ran for 73550 frames Cell Events: 3924 mean_circ: 0.935995
Ran for 74050 frames Cell Events: 3953 mean_circ: 0.936048
Ran for 75550 frames Cell Events: 4047 mean_circ: 0.935985
Ran for 78050 frames Cell Events: 4277 mean_circ: 0.935761
Ran for 79550 frames Cell Events: 4394 mean_circ: 0.93569
Ran for 80050 frames Cell Events: 4417 mean_circ: 0.935729
Ran for 80550 frames Cell Events: 4444 mean_circ: 0.935742
Ran for 81050 frames Cell Events: 4492 mean_circ: 0.935788
Ran for 82050 frames Cell Events: 4570 mean_circ: 0.935764
Ran for 83050 frames Cell Events: 4627 mean_circ: 0.935816
Ran for 83550 frames Cell Events: 4648 mean_circ: 0.935874
Ran for 84050 frames Cell Events: 4676 mean_circ: 0.935922
Ran for 85050 frames Cell Events: 4752 mean_circ: 0.935879
Ran for 85550 frames Cell Events: 4780 mean_circ: 0.935754
Ran for 86050 frames Cell Events: 4807 mean_circ: 0.935803
Ran for 87050 frames Cell Events: 4875 mean_circ: 0.935848
Ran for 88050 frames Cell Events: 4936 mean_circ: 0.935978
Ran for 88550 frames Cell Events: 4965 mean_circ: 0.935885
Ran for 89050 frames Cell Events: 4999 mean_circ: 0.935861
Ran for 90550 frames Cell Events: 5127 mean_circ: 0.935845
Ran for 91050 frames Cell Events: 5142 mean_circ: 0.935821
Ran for 92050 frames Cell Events: 5199 mean_circ: 0.935749
Ran for 92550 frames Cell Events: 5226 mean_circ: 0.935696
Ran for 93050 frames Cell Events: 5263 mean_circ: 0.935771
Ran for 94550 frames Cell Events: 5350 mean_circ: 0.935674
Ran for 95550 frames Cell Events: 5413 mean_circ: 0.935725
Ran for 96550 frames Cell Events: 5475 mean_circ: 0.935712
Ran for 97550 frames Cell Events: 5557 mean_circ: 0.935792
Ran for 100050 frames Cell Events: 5704 mean_circ: 0.935701
Ran for 101050 frames Cell Events: 5766 mean_circ: 0.935667
Ran for 104050 frames Cell Events: 5912 mean_circ: 0.935603
Ran for 105050 frames Cell Events: 5963 mean_circ: 0.935605
Ran for 105550 frames Cell Events: 5991 mean_circ: 0.93564
Ran for 106050 frames Cell Events: 6028 mean_circ: 0.93564
Ran for 106550 frames Cell Events: 6068 mean_circ: 0.935621
Ran for 107050 frames Cell Events: 6098 mean_circ: 0.935594
Ran for 107550 frames Cell Events: 6136 mean_circ: 0.935524
Ran for 110550 frames Cell Events: 6300 mean_circ: 0.935544
Ran for 113050 frames Cell Events: 6486 mean_circ: 0.935581
Ran for 113550 frames Cell Events: 6526 mean_circ: 0.935554
Ran for 114050 frames Cell Events: 6553 mean_circ: 0.935535
Ran for 115550 frames Cell Events: 6640 mean_circ: 0.93544
Ran for 116050 frames Cell Events: 6654 mean_circ: 0.935455
Ran for 116550 frames Cell Events: 6705 mean_circ: 0.935455
Ran for 117050 frames Cell Events: 6733 mean_circ: 0.935438
Ran for 118050 frames Cell Events: 6792 mean_circ: 0.93549
Ran for 118550 frames Cell Events: 6819 mean_circ: 0.935432
Ran for 119050 frames Cell Events: 6850 mean_circ: 0.935443
Ran for 119550 frames Cell Events: 6869 mean_circ: 0.935434
Ran for 120050 frames Cell Events: 6890 mean_circ: 0.93549
Ran for 120550 frames Cell Events: 6927 mean_circ: 0.935487
Ran for 121550 frames Cell Events: 6997 mean_circ: 0.93556
Ran for 122050 frames Cell Events: 7015 mean_circ: 0.935558
Ran for 122550 frames Cell Events: 7037 mean_circ: 0.93557
Ran for 123050 frames Cell Events: 7064 mean_circ: 0.935563
Ran for 124550 frames Cell Events: 7168 mean_circ: 0.935559
Ran for 125550 frames Cell Events: 7218 mean_circ: 0.935497
Ran for 128050 frames Cell Events: 7376 mean_circ: 0.935385
Ran for 129050 frames Cell Events: 7441 mean_circ: 0.935326
Ran for 129550 frames Cell Events: 7454 mean_circ: 0.935259
Ran for 130050 frames Cell Events: 7471 mean_circ: 0.935245
Ran for 130550 frames Cell Events: 7497 mean_circ: 0.935236
Ran for 131050 frames Cell Events: 7534 mean_circ: 0.935265
Ran for 131550 frames Cell Events: 7567 mean_circ: 0.935246
Ran for 132550 frames Cell Events: 7618 mean_circ: 0.93525
Ran for 133050 frames Cell Events: 7646 mean_circ: 0.935236
Ran for 133550 frames Cell Events: 7680 mean_circ: 0.935228
Ran for 134550 frames Cell Events: 7753 mean_circ: 0.935108
Ran for 135550 frames Cell Events: 7816 mean_circ: 0.935027
Ran for 137050 frames Cell Events: 7954 mean_circ: 0.934919
Ran for 138050 frames Cell Events: 8046 mean_circ: 0.934841
Ran for 139050 frames Cell Events: 8109 mean_circ: 0.934772
Ran for 141550 frames Cell Events: 8294 mean_circ: 0.934783
Ran for 142550 frames Cell Events: 8351 mean_circ: 0.934745
Ran for 143550 frames Cell Events: 8401 mean_circ: 0.934753
Ran for 144050 frames Cell Events: 8427 mean_circ: 0.934758
Ran for 145050 frames Cell Events: 8491 mean_circ: 0.934746
Ran for 145550 frames Cell Events: 8525 mean_circ: 0.93475
Ran for 147550 frames Cell Events: 8653 mean_circ: 0.934728
Ran for 149050 frames Cell Events: 8742 mean_circ: 0.93464
Ran for 149550 frames Cell Events: 8776 mean_circ: 0.934647
Ran for 150550 frames Cell Events: 8844 mean_circ: 0.934703
Ran for 151050 frames Cell Events: 8879 mean_circ: 0.934704
Ran for 151550 frames Cell Events: 8914 mean_circ: 0.934743
Ran for 153050 frames Cell Events: 9040 mean_circ: 0.93478
Ran for 155550 frames Cell Events: 9188 mean_circ: 0.934707
Ran for 156050 frames Cell Events: 9218 mean_circ: 0.934666
Ran for 157050 frames Cell Events: 9293 mean_circ: 0.934657



End this measurment..



Stopping time: 7.18289e+008
****************************************
Measurement finished

Mean Circularitiy: 0.934576
Mean Area: 598.254
Mean FL-1 intensity: 3068.71
Mean FL-2 intensity: 4510.83
Mean FL-3 intensity: 0

frames processed: 156482
Total Time: 79561.3ms
Number of Runs (new bg img) 0
Frames collected: 158990
Skipped frames: 62227
Rescued Frames: 2179324
Samples DAQ: 39748393
Samples written: 79494968
Samples per Channel: 39747484
FPS: 1998.33

Samples per second: 499583
Expected samples/s: 500000

Events: 9477
Events per second: 119.116
jumped back (PMT): 27
#Frames read, frequency
# 0 frames :238345
# 1 frames :64982
# 2 frames :2858
# 3 frames :1004
# 4 frames :442
# 5 frames :240
# 6 frames :3258
# 7 frames :1119
# 8 frames :1050
# 9 frames :558
# 10 frames :374
# 11 frames :241
# 12 frames :233
# 13 frames :224
# 14 frames :282
# 15 frames :195
# 16 frames :135
# 17 frames :92
# 18 frames :75
# 19 frames :71
# 20 frames :79
# 21 frames :50
# 22 frames :55
# 23 frames :35
# 24 frames :33
# 25 frames :29
# 26 frames :34
# 27 frames :24
# 28 frames :16
# 29 frames :21
# 30 frames :21
# 31 frames :12
# 32 frames :13
# 33 frames :8
# 34 frames :8
# 35 frames :9
# 36 frames :12
# 37 frames :11
# 38 frames :6
# 39 frames :3
# 40 frames :2
# 41 frames :5
# 42 frames :8
# 43 frames :3
# 44 frames :3
# 45 frames :3
# 46 frames :1
# 47 frames :3
# 48 frames :1
# 50 frames :3
# 51 frames :2
# 52 frames :2
# 53 frames :2
# 54 frames :3
# 55 frames :1
# 56 frames :1
# 59 frames :1
# 61 frames :1
# 63 frames :1
# 64 frames :1
# 68 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.1295
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0