Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-09_20.00.38 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "3dc28f85955f85f7f8f73c95c3ee27f2",
"name": "M1_20170523_1627_dsRNA_G1_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "18:00:57"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8046 (max 8047) in '8046'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.1585
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 7.20293e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 26 mean_circ: 0.93411
Ran for 2550 frames Cell Events: 134 mean_circ: 0.930535
Ran for 3050 frames Cell Events: 160 mean_circ: 0.930967
Ran for 3550 frames Cell Events: 186 mean_circ: 0.931273
Ran for 4050 frames Cell Events: 215 mean_circ: 0.933312
Ran for 5550 frames Cell Events: 254 mean_circ: 0.93218
Ran for 6050 frames Cell Events: 274 mean_circ: 0.932744
Ran for 6550 frames Cell Events: 299 mean_circ: 0.93375
Ran for 7050 frames Cell Events: 325 mean_circ: 0.933713
Ran for 8050 frames Cell Events: 375 mean_circ: 0.933721
Ran for 9550 frames Cell Events: 462 mean_circ: 0.932973
Ran for 11550 frames Cell Events: 583 mean_circ: 0.933142
Ran for 12050 frames Cell Events: 612 mean_circ: 0.933453
Ran for 13050 frames Cell Events: 665 mean_circ: 0.933853
Ran for 13550 frames Cell Events: 686 mean_circ: 0.933636
Ran for 14050 frames Cell Events: 704 mean_circ: 0.933783
Ran for 14550 frames Cell Events: 733 mean_circ: 0.933969
Ran for 15050 frames Cell Events: 769 mean_circ: 0.934344
Ran for 15550 frames Cell Events: 797 mean_circ: 0.934672
Ran for 16550 frames Cell Events: 853 mean_circ: 0.934518
Ran for 18050 frames Cell Events: 915 mean_circ: 0.934613
Ran for 20050 frames Cell Events: 1033 mean_circ: 0.934219
Ran for 20550 frames Cell Events: 1054 mean_circ: 0.934071
Ran for 21550 frames Cell Events: 1101 mean_circ: 0.933793
Ran for 23050 frames Cell Events: 1254 mean_circ: 0.933766
Ran for 23550 frames Cell Events: 1301 mean_circ: 0.933766
Ran for 24050 frames Cell Events: 1324 mean_circ: 0.933741
Ran for 24550 frames Cell Events: 1362 mean_circ: 0.933479
Ran for 25050 frames Cell Events: 1396 mean_circ: 0.93354
Ran for 25550 frames Cell Events: 1435 mean_circ: 0.93338
Ran for 26550 frames Cell Events: 1483 mean_circ: 0.933397
Ran for 27550 frames Cell Events: 1528 mean_circ: 0.933482
Ran for 28050 frames Cell Events: 1550 mean_circ: 0.933418
Ran for 28550 frames Cell Events: 1574 mean_circ: 0.933247
Ran for 29050 frames Cell Events: 1604 mean_circ: 0.933309
Ran for 30550 frames Cell Events: 1707 mean_circ: 0.933329
Ran for 31050 frames Cell Events: 1733 mean_circ: 0.93336
Ran for 32050 frames Cell Events: 1776 mean_circ: 0.933425
Ran for 33050 frames Cell Events: 1825 mean_circ: 0.933434
Ran for 33550 frames Cell Events: 1852 mean_circ: 0.933488
Ran for 34050 frames Cell Events: 1876 mean_circ: 0.933525
Ran for 34550 frames Cell Events: 1904 mean_circ: 0.933527
Ran for 36550 frames Cell Events: 2047 mean_circ: 0.933127
Ran for 38050 frames Cell Events: 2145 mean_circ: 0.933036
Ran for 38550 frames Cell Events: 2175 mean_circ: 0.932832
Ran for 39050 frames Cell Events: 2206 mean_circ: 0.932859
Ran for 39550 frames Cell Events: 2225 mean_circ: 0.93297
Ran for 41550 frames Cell Events: 2327 mean_circ: 0.933098
Ran for 42550 frames Cell Events: 2382 mean_circ: 0.933237
Ran for 45550 frames Cell Events: 2539 mean_circ: 0.933407
Ran for 46550 frames Cell Events: 2590 mean_circ: 0.933662
Ran for 47050 frames Cell Events: 2606 mean_circ: 0.933645
Ran for 48050 frames Cell Events: 2654 mean_circ: 0.933802
Ran for 48550 frames Cell Events: 2680 mean_circ: 0.933788
Ran for 50050 frames Cell Events: 2794 mean_circ: 0.933828
Ran for 50550 frames Cell Events: 2821 mean_circ: 0.933773
Ran for 51050 frames Cell Events: 2847 mean_circ: 0.933711
Ran for 51550 frames Cell Events: 2868 mean_circ: 0.933805
Ran for 52050 frames Cell Events: 2903 mean_circ: 0.933866
Ran for 52550 frames Cell Events: 2936 mean_circ: 0.933845
Ran for 53550 frames Cell Events: 3020 mean_circ: 0.933847
Ran for 54050 frames Cell Events: 3045 mean_circ: 0.933815
Ran for 54550 frames Cell Events: 3070 mean_circ: 0.933792
Ran for 55050 frames Cell Events: 3090 mean_circ: 0.933748
Ran for 55550 frames Cell Events: 3104 mean_circ: 0.933761
Ran for 56050 frames Cell Events: 3142 mean_circ: 0.933746
Ran for 56550 frames Cell Events: 3186 mean_circ: 0.933759
Ran for 57550 frames Cell Events: 3252 mean_circ: 0.933706
Ran for 58050 frames Cell Events: 3281 mean_circ: 0.933651
Ran for 59050 frames Cell Events: 3347 mean_circ: 0.933577
Ran for 59550 frames Cell Events: 3373 mean_circ: 0.933666
Ran for 61050 frames Cell Events: 3460 mean_circ: 0.933702
Ran for 61550 frames Cell Events: 3486 mean_circ: 0.933696
Ran for 64050 frames Cell Events: 3626 mean_circ: 0.933597
Ran for 64550 frames Cell Events: 3660 mean_circ: 0.933598
Ran for 65050 frames Cell Events: 3688 mean_circ: 0.93352
Ran for 65550 frames Cell Events: 3717 mean_circ: 0.933536
Ran for 66550 frames Cell Events: 3789 mean_circ: 0.933431
Ran for 67550 frames Cell Events: 3902 mean_circ: 0.933535
Ran for 68050 frames Cell Events: 3928 mean_circ: 0.933653
Ran for 68550 frames Cell Events: 3953 mean_circ: 0.933696
Ran for 70050 frames Cell Events: 4049 mean_circ: 0.93349
Ran for 70550 frames Cell Events: 4075 mean_circ: 0.933442
Ran for 73050 frames Cell Events: 4238 mean_circ: 0.93349
Ran for 75050 frames Cell Events: 4334 mean_circ: 0.933397
Ran for 75550 frames Cell Events: 4373 mean_circ: 0.933427
Ran for 77550 frames Cell Events: 4511 mean_circ: 0.933392
Ran for 78050 frames Cell Events: 4543 mean_circ: 0.933424
Ran for 78550 frames Cell Events: 4564 mean_circ: 0.933471
Ran for 79050 frames Cell Events: 4575 mean_circ: 0.933476
Ran for 79550 frames Cell Events: 4601 mean_circ: 0.933483
Ran for 81050 frames Cell Events: 4678 mean_circ: 0.933377
Ran for 82050 frames Cell Events: 4739 mean_circ: 0.93339
Ran for 83050 frames Cell Events: 4797 mean_circ: 0.933461
Ran for 83550 frames Cell Events: 4815 mean_circ: 0.933442
Ran for 84550 frames Cell Events: 4883 mean_circ: 0.933363
Ran for 85050 frames Cell Events: 4913 mean_circ: 0.933398
Ran for 85550 frames Cell Events: 4928 mean_circ: 0.933406
Ran for 86050 frames Cell Events: 4950 mean_circ: 0.933444
Ran for 86550 frames Cell Events: 4974 mean_circ: 0.933507
Ran for 88050 frames Cell Events: 5057 mean_circ: 0.933603
Ran for 88550 frames Cell Events: 5091 mean_circ: 0.93362
Ran for 89550 frames Cell Events: 5155 mean_circ: 0.933692
Ran for 92550 frames Cell Events: 5306 mean_circ: 0.93373
Ran for 93550 frames Cell Events: 5380 mean_circ: 0.933697
Ran for 94050 frames Cell Events: 5407 mean_circ: 0.933743
Ran for 95050 frames Cell Events: 5480 mean_circ: 0.933773
Ran for 98050 frames Cell Events: 5683 mean_circ: 0.933884
Ran for 105050 frames Cell Events: 6068 mean_circ: 0.934187
Ran for 105550 frames Cell Events: 6087 mean_circ: 0.934165
Ran for 106050 frames Cell Events: 6110 mean_circ: 0.934191
Ran for 106550 frames Cell Events: 6148 mean_circ: 0.934191
Ran for 107050 frames Cell Events: 6172 mean_circ: 0.934201
Ran for 108050 frames Cell Events: 6228 mean_circ: 0.934215
Ran for 109050 frames Cell Events: 6280 mean_circ: 0.934324
Ran for 110050 frames Cell Events: 6329 mean_circ: 0.934323
Ran for 110550 frames Cell Events: 6345 mean_circ: 0.934352
Ran for 112550 frames Cell Events: 6495 mean_circ: 0.934556
Ran for 113050 frames Cell Events: 6521 mean_circ: 0.934581
Ran for 113550 frames Cell Events: 6547 mean_circ: 0.934634
Ran for 114050 frames Cell Events: 6582 mean_circ: 0.934649
Ran for 115050 frames Cell Events: 6647 mean_circ: 0.934636
Ran for 115550 frames Cell Events: 6676 mean_circ: 0.934669
Ran for 116050 frames Cell Events: 6705 mean_circ: 0.93462
Ran for 116550 frames Cell Events: 6737 mean_circ: 0.934555
Ran for 120550 frames Cell Events: 7141 mean_circ: 0.934222
Ran for 121050 frames Cell Events: 7163 mean_circ: 0.934234
Ran for 123050 frames Cell Events: 7304 mean_circ: 0.934184
Ran for 126550 frames Cell Events: 7590 mean_circ: 0.934111
Ran for 127050 frames Cell Events: 7611 mean_circ: 0.934119
Ran for 128050 frames Cell Events: 7678 mean_circ: 0.934168
Ran for 128550 frames Cell Events: 7701 mean_circ: 0.934127
Ran for 129050 frames Cell Events: 7733 mean_circ: 0.934086
Ran for 130550 frames Cell Events: 7848 mean_circ: 0.934006
Ran for 132550 frames Cell Events: 7959 mean_circ: 0.93409
Ran for 133050 frames Cell Events: 7980 mean_circ: 0.934096
Ran for 134050 frames Cell Events: 8045 mean_circ: 0.934098



End this measurment..



Stopping time: 7.2036e+008
****************************************
Measurement finished

Mean Circularitiy: 0.934101
Mean Area: 658.064
Mean FL-1 intensity: 3908.08
Mean FL-2 intensity: 5294.01
Mean FL-3 intensity: 0

frames processed: 130876
Total Time: 67114.4ms
Number of Runs (new bg img) 0
Frames collected: 134110
Skipped frames: 77396
Rescued Frames: 2676588
Samples DAQ: 33530453
Samples written: 67054976
Samples per Channel: 33527488
FPS: 1998.23

Samples per second: 499557
Expected samples/s: 500000

Events: 8047
Events per second: 119.9
jumped back (PMT): 17
#Frames read, frequency
# 0 frames :205399
# 1 frames :51071
# 2 frames :2582
# 3 frames :850
# 4 frames :471
# 5 frames :169
# 6 frames :2127
# 7 frames :1024
# 8 frames :931
# 9 frames :537
# 10 frames :440
# 11 frames :226
# 12 frames :201
# 13 frames :140
# 14 frames :235
# 15 frames :176
# 16 frames :142
# 17 frames :101
# 18 frames :60
# 19 frames :54
# 20 frames :63
# 21 frames :56
# 22 frames :39
# 23 frames :36
# 24 frames :32
# 25 frames :33
# 26 frames :20
# 27 frames :15
# 28 frames :28
# 29 frames :22
# 30 frames :22
# 31 frames :16
# 32 frames :10
# 33 frames :7
# 34 frames :15
# 35 frames :12
# 36 frames :11
# 37 frames :8
# 38 frames :8
# 39 frames :8
# 40 frames :4
# 41 frames :7
# 42 frames :7
# 43 frames :4
# 44 frames :1
# 45 frames :2
# 46 frames :3
# 47 frames :7
# 48 frames :5
# 49 frames :3
# 50 frames :2
# 51 frames :3
# 52 frames :2
# 53 frames :1
# 54 frames :4
# 56 frames :1
# 57 frames :2
# 58 frames :1
# 59 frames :2
# 61 frames :3
# 62 frames :3
# 63 frames :1
# 70 frames :1
# 81 frames :1
# 82 frames :1
# 96 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.1585
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0