Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-09_20.02.30 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "77c25e6348412f75f640286c4bff7c24",
"name": "M1_20170523_1653_dsRNA_LZ_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "18:02:54"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 9410 (max 9411) in '9410'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.1599
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 7.21869e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 51 mean_circ: 0.922661
Ran for 1550 frames Cell Events: 85 mean_circ: 0.922661
Ran for 2050 frames Cell Events: 110 mean_circ: 0.92011
Ran for 4050 frames Cell Events: 255 mean_circ: 0.922792
Ran for 6050 frames Cell Events: 342 mean_circ: 0.925342
Ran for 7550 frames Cell Events: 436 mean_circ: 0.928949
Ran for 8050 frames Cell Events: 458 mean_circ: 0.929434
Ran for 9050 frames Cell Events: 513 mean_circ: 0.9298
Ran for 9550 frames Cell Events: 537 mean_circ: 0.930707
Ran for 10050 frames Cell Events: 564 mean_circ: 0.931368
Ran for 11550 frames Cell Events: 646 mean_circ: 0.931835
Ran for 12050 frames Cell Events: 681 mean_circ: 0.931509
Ran for 14050 frames Cell Events: 797 mean_circ: 0.930684
Ran for 15550 frames Cell Events: 868 mean_circ: 0.930605
Ran for 17550 frames Cell Events: 1004 mean_circ: 0.929835
Ran for 19050 frames Cell Events: 1092 mean_circ: 0.930885
Ran for 19550 frames Cell Events: 1123 mean_circ: 0.9313
Ran for 21550 frames Cell Events: 1241 mean_circ: 0.931441
Ran for 22550 frames Cell Events: 1309 mean_circ: 0.931382
Ran for 24050 frames Cell Events: 1411 mean_circ: 0.930883
Ran for 26550 frames Cell Events: 1574 mean_circ: 0.930825
Ran for 27050 frames Cell Events: 1605 mean_circ: 0.930903
Ran for 29550 frames Cell Events: 1735 mean_circ: 0.931073
Ran for 30050 frames Cell Events: 1769 mean_circ: 0.931254
Ran for 30550 frames Cell Events: 1799 mean_circ: 0.931089
Ran for 31550 frames Cell Events: 1863 mean_circ: 0.930694
Ran for 32050 frames Cell Events: 1890 mean_circ: 0.930662
Ran for 33550 frames Cell Events: 1988 mean_circ: 0.930364
Ran for 34550 frames Cell Events: 2066 mean_circ: 0.929971
Ran for 36050 frames Cell Events: 2161 mean_circ: 0.929693
Ran for 36550 frames Cell Events: 2184 mean_circ: 0.929873
Ran for 37550 frames Cell Events: 2243 mean_circ: 0.930132
Ran for 38550 frames Cell Events: 2301 mean_circ: 0.93021
Ran for 39050 frames Cell Events: 2325 mean_circ: 0.93029
Ran for 39550 frames Cell Events: 2363 mean_circ: 0.930341
Ran for 40050 frames Cell Events: 2389 mean_circ: 0.930149
Ran for 40550 frames Cell Events: 2418 mean_circ: 0.930145
Ran for 41050 frames Cell Events: 2442 mean_circ: 0.930314
Ran for 43550 frames Cell Events: 2594 mean_circ: 0.930087
Ran for 44550 frames Cell Events: 2641 mean_circ: 0.92984
Ran for 45050 frames Cell Events: 2666 mean_circ: 0.929643
Ran for 47550 frames Cell Events: 2838 mean_circ: 0.929665
Ran for 48050 frames Cell Events: 2859 mean_circ: 0.929797
Ran for 48550 frames Cell Events: 2876 mean_circ: 0.929845
Ran for 49050 frames Cell Events: 2906 mean_circ: 0.929887
Ran for 49550 frames Cell Events: 2946 mean_circ: 0.929715
Ran for 50050 frames Cell Events: 2974 mean_circ: 0.929668
Ran for 51050 frames Cell Events: 3049 mean_circ: 0.929618
Ran for 52550 frames Cell Events: 3145 mean_circ: 0.929369
Ran for 54050 frames Cell Events: 3235 mean_circ: 0.929517
Ran for 55050 frames Cell Events: 3282 mean_circ: 0.929401
Ran for 56050 frames Cell Events: 3324 mean_circ: 0.929431
Ran for 57550 frames Cell Events: 3432 mean_circ: 0.929355
Ran for 58550 frames Cell Events: 3490 mean_circ: 0.929393
Ran for 60050 frames Cell Events: 3602 mean_circ: 0.929321
Ran for 61050 frames Cell Events: 3664 mean_circ: 0.929247
Ran for 62550 frames Cell Events: 3748 mean_circ: 0.929057
Ran for 65050 frames Cell Events: 3922 mean_circ: 0.929131
Ran for 65550 frames Cell Events: 3943 mean_circ: 0.929153
Ran for 66550 frames Cell Events: 4008 mean_circ: 0.929
Ran for 67050 frames Cell Events: 4021 mean_circ: 0.928912
Ran for 68550 frames Cell Events: 4113 mean_circ: 0.928846
Ran for 71550 frames Cell Events: 4295 mean_circ: 0.928584
Ran for 72050 frames Cell Events: 4317 mean_circ: 0.92864
Ran for 74050 frames Cell Events: 4461 mean_circ: 0.928605
Ran for 75550 frames Cell Events: 4558 mean_circ: 0.928616
Ran for 76550 frames Cell Events: 4617 mean_circ: 0.928681
Ran for 77050 frames Cell Events: 4644 mean_circ: 0.928698
Ran for 78050 frames Cell Events: 4697 mean_circ: 0.928677
Ran for 78550 frames Cell Events: 4730 mean_circ: 0.928669
Ran for 79050 frames Cell Events: 4745 mean_circ: 0.928706
Ran for 79550 frames Cell Events: 4771 mean_circ: 0.928706
Ran for 80050 frames Cell Events: 4799 mean_circ: 0.928715
Ran for 80550 frames Cell Events: 4840 mean_circ: 0.928672
Ran for 81050 frames Cell Events: 4871 mean_circ: 0.928669
Ran for 81550 frames Cell Events: 4901 mean_circ: 0.928672
Ran for 82050 frames Cell Events: 4930 mean_circ: 0.928613
Ran for 82550 frames Cell Events: 4955 mean_circ: 0.928649
Ran for 84050 frames Cell Events: 5058 mean_circ: 0.928664
Ran for 85550 frames Cell Events: 5167 mean_circ: 0.928825
Ran for 88050 frames Cell Events: 5329 mean_circ: 0.928894
Ran for 89550 frames Cell Events: 5414 mean_circ: 0.92895
Ran for 90050 frames Cell Events: 5447 mean_circ: 0.928886
Ran for 90550 frames Cell Events: 5477 mean_circ: 0.928916
Ran for 91550 frames Cell Events: 5538 mean_circ: 0.929041
Ran for 93550 frames Cell Events: 5669 mean_circ: 0.928988
Ran for 94050 frames Cell Events: 5693 mean_circ: 0.929097
Ran for 94550 frames Cell Events: 5719 mean_circ: 0.929118
Ran for 95050 frames Cell Events: 5744 mean_circ: 0.929135
Ran for 95550 frames Cell Events: 5761 mean_circ: 0.929157
Ran for 96550 frames Cell Events: 5815 mean_circ: 0.929135
Ran for 97550 frames Cell Events: 5878 mean_circ: 0.929117
Ran for 99050 frames Cell Events: 5970 mean_circ: 0.92928
Ran for 100050 frames Cell Events: 6042 mean_circ: 0.92924
Ran for 100550 frames Cell Events: 6076 mean_circ: 0.929194
Ran for 101550 frames Cell Events: 6138 mean_circ: 0.929192
Ran for 102050 frames Cell Events: 6175 mean_circ: 0.929221
Ran for 102550 frames Cell Events: 6203 mean_circ: 0.929274
Ran for 105550 frames Cell Events: 6425 mean_circ: 0.929121
Ran for 108050 frames Cell Events: 6609 mean_circ: 0.929159
Ran for 111550 frames Cell Events: 6874 mean_circ: 0.929272
Ran for 113550 frames Cell Events: 7021 mean_circ: 0.929313
Ran for 115050 frames Cell Events: 7154 mean_circ: 0.92938
Ran for 115550 frames Cell Events: 7173 mean_circ: 0.929372
Ran for 116050 frames Cell Events: 7195 mean_circ: 0.929369
Ran for 116550 frames Cell Events: 7221 mean_circ: 0.929337
Ran for 118050 frames Cell Events: 7323 mean_circ: 0.929328
Ran for 118550 frames Cell Events: 7363 mean_circ: 0.929355
Ran for 120050 frames Cell Events: 7460 mean_circ: 0.929458
Ran for 121550 frames Cell Events: 7567 mean_circ: 0.929391
Ran for 122050 frames Cell Events: 7590 mean_circ: 0.929331
Ran for 123550 frames Cell Events: 7691 mean_circ: 0.929215
Ran for 126550 frames Cell Events: 7865 mean_circ: 0.929013
Ran for 127050 frames Cell Events: 7885 mean_circ: 0.929021
Ran for 127550 frames Cell Events: 7917 mean_circ: 0.928957
Ran for 128050 frames Cell Events: 7954 mean_circ: 0.928979
Ran for 129050 frames Cell Events: 8000 mean_circ: 0.929043
Ran for 131050 frames Cell Events: 8141 mean_circ: 0.929017
Ran for 131550 frames Cell Events: 8175 mean_circ: 0.92903
Ran for 132550 frames Cell Events: 8242 mean_circ: 0.928943
Ran for 134550 frames Cell Events: 8352 mean_circ: 0.928823
Ran for 135050 frames Cell Events: 8383 mean_circ: 0.928837
Ran for 138550 frames Cell Events: 8579 mean_circ: 0.928985
Ran for 139050 frames Cell Events: 8608 mean_circ: 0.929014
Ran for 139550 frames Cell Events: 8646 mean_circ: 0.929069
Ran for 140050 frames Cell Events: 8658 mean_circ: 0.929083
Ran for 141050 frames Cell Events: 8708 mean_circ: 0.929123
Ran for 141550 frames Cell Events: 8737 mean_circ: 0.92915
Ran for 143050 frames Cell Events: 8827 mean_circ: 0.929197
Ran for 143550 frames Cell Events: 8863 mean_circ: 0.929209
Ran for 144550 frames Cell Events: 8941 mean_circ: 0.929228
Ran for 145050 frames Cell Events: 8982 mean_circ: 0.929259
Ran for 146550 frames Cell Events: 9084 mean_circ: 0.929218
Ran for 149050 frames Cell Events: 9188 mean_circ: 0.929233
Ran for 149550 frames Cell Events: 9213 mean_circ: 0.929219
Ran for 150050 frames Cell Events: 9239 mean_circ: 0.929233
Ran for 150550 frames Cell Events: 9264 mean_circ: 0.929265
Ran for 151050 frames Cell Events: 9290 mean_circ: 0.929333
Ran for 152050 frames Cell Events: 9359 mean_circ: 0.929345
Ran for 152550 frames Cell Events: 9393 mean_circ: 0.929345



End this measurment..



Stopping time: 7.21945e+008
****************************************
Measurement finished

Mean Circularitiy: 0.929341
Mean Area: 654.402
Mean FL-1 intensity: 3405.39
Mean FL-2 intensity: 4863.35
Mean FL-3 intensity: 0

frames processed: 149110
Total Time: 76471.7ms
Number of Runs (new bg img) 0
Frames collected: 152825
Skipped frames: 88868
Rescued Frames: 3027783
Samples DAQ: 38209119
Samples written: 76412656
Samples per Channel: 38206328
FPS: 1998.45

Samples per second: 499614
Expected samples/s: 500000

Events: 9411
Events per second: 123.065
jumped back (PMT): 27
#Frames read, frequency
# 0 frames :237812
# 1 frames :55325
# 2 frames :2614
# 3 frames :1065
# 4 frames :460
# 5 frames :225
# 6 frames :2461
# 7 frames :1141
# 8 frames :1102
# 9 frames :625
# 10 frames :389
# 11 frames :296
# 12 frames :249
# 13 frames :194
# 14 frames :307
# 15 frames :210
# 16 frames :160
# 17 frames :129
# 18 frames :97
# 19 frames :74
# 20 frames :85
# 21 frames :73
# 22 frames :53
# 23 frames :54
# 24 frames :36
# 25 frames :50
# 26 frames :42
# 27 frames :31
# 28 frames :27
# 29 frames :27
# 30 frames :16
# 31 frames :26
# 32 frames :17
# 33 frames :10
# 34 frames :9
# 35 frames :11
# 36 frames :10
# 37 frames :11
# 38 frames :8
# 39 frames :4
# 40 frames :5
# 41 frames :5
# 42 frames :8
# 43 frames :6
# 44 frames :2
# 45 frames :3
# 46 frames :3
# 47 frames :4
# 48 frames :1
# 49 frames :3
# 50 frames :3
# 51 frames :1
# 52 frames :1
# 53 frames :4
# 54 frames :2
# 55 frames :1
# 57 frames :5
# 58 frames :1
# 59 frames :1
# 60 frames :2
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# 63 frames :1
# 64 frames :2
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# 78 frames :1
# 82 frames :1
# 90 frames :1
# 91 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.1599
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0