Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-09_20.05.47 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "e8c4db80dda65205cea8a910bb0392c1",
"name": "M1_20170523_1805_dsRNA_H3_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "18:06:07"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8092 (max 8093) in '8092'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.1968
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 7.26183e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 25 mean_circ: 0.952953
Ran for 1050 frames Cell Events: 53 mean_circ: 0.946728
Ran for 1550 frames Cell Events: 76 mean_circ: 0.948165
Ran for 2050 frames Cell Events: 100 mean_circ: 0.945048
Ran for 2550 frames Cell Events: 121 mean_circ: 0.946294
Ran for 4050 frames Cell Events: 205 mean_circ: 0.945882
Ran for 5050 frames Cell Events: 241 mean_circ: 0.945014
Ran for 6550 frames Cell Events: 314 mean_circ: 0.946765
Ran for 8050 frames Cell Events: 401 mean_circ: 0.947167
Ran for 8550 frames Cell Events: 422 mean_circ: 0.947075
Ran for 9050 frames Cell Events: 455 mean_circ: 0.946148
Ran for 9550 frames Cell Events: 482 mean_circ: 0.946206
Ran for 10050 frames Cell Events: 507 mean_circ: 0.946536
Ran for 11050 frames Cell Events: 568 mean_circ: 0.94622
Ran for 12050 frames Cell Events: 634 mean_circ: 0.946603
Ran for 14550 frames Cell Events: 787 mean_circ: 0.946323
Ran for 15050 frames Cell Events: 822 mean_circ: 0.946735
Ran for 17050 frames Cell Events: 926 mean_circ: 0.947219
Ran for 18050 frames Cell Events: 995 mean_circ: 0.946705
Ran for 18550 frames Cell Events: 1022 mean_circ: 0.946718
Ran for 19050 frames Cell Events: 1037 mean_circ: 0.946904
Ran for 20050 frames Cell Events: 1098 mean_circ: 0.947165
Ran for 21050 frames Cell Events: 1166 mean_circ: 0.94687
Ran for 24050 frames Cell Events: 1359 mean_circ: 0.947419
Ran for 24550 frames Cell Events: 1386 mean_circ: 0.947398
Ran for 25550 frames Cell Events: 1439 mean_circ: 0.947161
Ran for 26050 frames Cell Events: 1458 mean_circ: 0.947002
Ran for 28550 frames Cell Events: 1594 mean_circ: 0.946978
Ran for 29050 frames Cell Events: 1625 mean_circ: 0.946992
Ran for 29550 frames Cell Events: 1659 mean_circ: 0.947011
Ran for 30550 frames Cell Events: 1731 mean_circ: 0.947138
Ran for 31050 frames Cell Events: 1766 mean_circ: 0.947005
Ran for 31550 frames Cell Events: 1801 mean_circ: 0.947075
Ran for 32550 frames Cell Events: 1847 mean_circ: 0.947069
Ran for 33550 frames Cell Events: 1896 mean_circ: 0.947202
Ran for 34550 frames Cell Events: 1959 mean_circ: 0.946913
Ran for 37550 frames Cell Events: 2157 mean_circ: 0.945848
Ran for 38050 frames Cell Events: 2176 mean_circ: 0.945977
Ran for 39550 frames Cell Events: 2295 mean_circ: 0.94619
Ran for 40550 frames Cell Events: 2364 mean_circ: 0.945644
Ran for 42050 frames Cell Events: 2469 mean_circ: 0.945622
Ran for 43050 frames Cell Events: 2523 mean_circ: 0.945635
Ran for 44050 frames Cell Events: 2572 mean_circ: 0.945534
Ran for 45550 frames Cell Events: 2642 mean_circ: 0.94547
Ran for 46550 frames Cell Events: 2679 mean_circ: 0.945504
Ran for 47050 frames Cell Events: 2704 mean_circ: 0.945604
Ran for 48050 frames Cell Events: 2749 mean_circ: 0.945627
Ran for 49550 frames Cell Events: 2821 mean_circ: 0.945692
Ran for 50050 frames Cell Events: 2845 mean_circ: 0.945652
Ran for 51050 frames Cell Events: 2896 mean_circ: 0.945575
Ran for 51550 frames Cell Events: 2927 mean_circ: 0.945548
Ran for 53550 frames Cell Events: 3048 mean_circ: 0.945809
Ran for 54050 frames Cell Events: 3074 mean_circ: 0.945879
Ran for 54550 frames Cell Events: 3098 mean_circ: 0.946028
Ran for 56550 frames Cell Events: 3220 mean_circ: 0.946101
Ran for 57050 frames Cell Events: 3245 mean_circ: 0.946114
Ran for 58050 frames Cell Events: 3304 mean_circ: 0.94605
Ran for 59550 frames Cell Events: 3381 mean_circ: 0.946207
Ran for 60550 frames Cell Events: 3424 mean_circ: 0.946072
Ran for 62550 frames Cell Events: 3557 mean_circ: 0.946028
Ran for 65050 frames Cell Events: 3706 mean_circ: 0.946032
Ran for 66050 frames Cell Events: 3768 mean_circ: 0.945942
Ran for 66550 frames Cell Events: 3779 mean_circ: 0.945968
Ran for 67050 frames Cell Events: 3814 mean_circ: 0.945908
Ran for 68550 frames Cell Events: 3884 mean_circ: 0.945963
Ran for 69050 frames Cell Events: 3898 mean_circ: 0.945982
Ran for 69550 frames Cell Events: 3927 mean_circ: 0.946024
Ran for 70050 frames Cell Events: 3937 mean_circ: 0.946079
Ran for 70550 frames Cell Events: 3966 mean_circ: 0.946014
Ran for 71050 frames Cell Events: 3988 mean_circ: 0.945947
Ran for 71550 frames Cell Events: 4006 mean_circ: 0.945931
Ran for 72550 frames Cell Events: 4049 mean_circ: 0.946002
Ran for 74050 frames Cell Events: 4142 mean_circ: 0.946024
Ran for 75550 frames Cell Events: 4223 mean_circ: 0.945999
Ran for 76050 frames Cell Events: 4242 mean_circ: 0.945977
Ran for 76550 frames Cell Events: 4255 mean_circ: 0.946031
Ran for 77050 frames Cell Events: 4279 mean_circ: 0.946025
Ran for 78550 frames Cell Events: 4368 mean_circ: 0.946124
Ran for 79550 frames Cell Events: 4416 mean_circ: 0.946166
Ran for 81550 frames Cell Events: 4529 mean_circ: 0.94625
Ran for 82050 frames Cell Events: 4564 mean_circ: 0.946282
Ran for 82550 frames Cell Events: 4595 mean_circ: 0.946325
Ran for 83550 frames Cell Events: 4645 mean_circ: 0.946346
Ran for 84050 frames Cell Events: 4667 mean_circ: 0.946378
Ran for 84550 frames Cell Events: 4685 mean_circ: 0.946412
Ran for 85050 frames Cell Events: 4704 mean_circ: 0.94644
Ran for 85550 frames Cell Events: 4719 mean_circ: 0.946378
Ran for 88550 frames Cell Events: 4855 mean_circ: 0.946155
Ran for 89050 frames Cell Events: 4870 mean_circ: 0.946147
Ran for 89550 frames Cell Events: 4891 mean_circ: 0.946202
Ran for 90550 frames Cell Events: 4951 mean_circ: 0.946121
Ran for 91050 frames Cell Events: 4975 mean_circ: 0.946103
Ran for 91550 frames Cell Events: 4992 mean_circ: 0.946084
Ran for 92050 frames Cell Events: 5007 mean_circ: 0.946079
Ran for 94550 frames Cell Events: 5147 mean_circ: 0.946054
Ran for 95550 frames Cell Events: 5193 mean_circ: 0.945984
Ran for 97550 frames Cell Events: 5310 mean_circ: 0.945986
Ran for 98050 frames Cell Events: 5329 mean_circ: 0.945959
Ran for 99050 frames Cell Events: 5383 mean_circ: 0.94574
Ran for 99550 frames Cell Events: 5411 mean_circ: 0.945732
Ran for 100050 frames Cell Events: 5439 mean_circ: 0.945731
Ran for 100550 frames Cell Events: 5461 mean_circ: 0.945775
Ran for 101050 frames Cell Events: 5485 mean_circ: 0.945728
Ran for 102050 frames Cell Events: 5522 mean_circ: 0.945748
Ran for 103550 frames Cell Events: 5589 mean_circ: 0.945794
Ran for 104550 frames Cell Events: 5648 mean_circ: 0.945802
Ran for 105050 frames Cell Events: 5678 mean_circ: 0.945819
Ran for 105550 frames Cell Events: 5690 mean_circ: 0.945809
Ran for 106050 frames Cell Events: 5717 mean_circ: 0.945769
Ran for 106550 frames Cell Events: 5740 mean_circ: 0.945776
Ran for 107050 frames Cell Events: 5770 mean_circ: 0.945812
Ran for 108050 frames Cell Events: 5806 mean_circ: 0.945816
Ran for 108550 frames Cell Events: 5829 mean_circ: 0.945831
Ran for 110550 frames Cell Events: 5945 mean_circ: 0.945883
Ran for 111550 frames Cell Events: 6000 mean_circ: 0.945876
Ran for 112550 frames Cell Events: 6054 mean_circ: 0.945874
Ran for 113550 frames Cell Events: 6097 mean_circ: 0.94593
Ran for 114550 frames Cell Events: 6142 mean_circ: 0.945981
Ran for 115050 frames Cell Events: 6166 mean_circ: 0.945996
Ran for 115550 frames Cell Events: 6196 mean_circ: 0.946011
Ran for 117050 frames Cell Events: 6276 mean_circ: 0.946024
Ran for 118050 frames Cell Events: 6330 mean_circ: 0.946018
Ran for 118550 frames Cell Events: 6351 mean_circ: 0.945947
Ran for 120550 frames Cell Events: 6467 mean_circ: 0.946079
Ran for 123050 frames Cell Events: 6631 mean_circ: 0.946039
Ran for 123550 frames Cell Events: 6649 mean_circ: 0.946049
Ran for 124050 frames Cell Events: 6674 mean_circ: 0.946
Ran for 125550 frames Cell Events: 6779 mean_circ: 0.945986
Ran for 126050 frames Cell Events: 6805 mean_circ: 0.946006
Ran for 126550 frames Cell Events: 6833 mean_circ: 0.945982
Ran for 127050 frames Cell Events: 6863 mean_circ: 0.945964
Ran for 128550 frames Cell Events: 6960 mean_circ: 0.946015
Ran for 129050 frames Cell Events: 6980 mean_circ: 0.94603
Ran for 129550 frames Cell Events: 7012 mean_circ: 0.946016
Ran for 130050 frames Cell Events: 7036 mean_circ: 0.946039
Ran for 131050 frames Cell Events: 7113 mean_circ: 0.946047
Ran for 133050 frames Cell Events: 7219 mean_circ: 0.945987
Ran for 133550 frames Cell Events: 7250 mean_circ: 0.945943
Ran for 134050 frames Cell Events: 7270 mean_circ: 0.945992
Ran for 134550 frames Cell Events: 7300 mean_circ: 0.945987
Ran for 136550 frames Cell Events: 7479 mean_circ: 0.945931
Ran for 137050 frames Cell Events: 7503 mean_circ: 0.945936
Ran for 138550 frames Cell Events: 7604 mean_circ: 0.945764
Ran for 140050 frames Cell Events: 7698 mean_circ: 0.94576
Ran for 141550 frames Cell Events: 7789 mean_circ: 0.94576
Ran for 143550 frames Cell Events: 7929 mean_circ: 0.945738
Ran for 145050 frames Cell Events: 8023 mean_circ: 0.945782
Ran for 146050 frames Cell Events: 8089 mean_circ: 0.945837



End this measurment..



Stopping time: 7.26256e+008
****************************************
Measurement finished

Mean Circularitiy: 0.945714
Mean Area: 603.478
Mean FL-1 intensity: 3891.15
Mean FL-2 intensity: 4855.07
Mean FL-3 intensity: 0

frames processed: 145399
Total Time: 73153.4ms
Number of Runs (new bg img) 0
Frames collected: 146120
Skipped frames: 103194
Rescued Frames: 3624042
Samples DAQ: 36530500
Samples written: 73059758
Samples per Channel: 36529879
FPS: 1997.45

Samples per second: 499360
Expected samples/s: 500000

Events: 8094
Events per second: 110.644
jumped back (PMT): 3227
#Frames read, frequency
# 0 frames :225438
# 1 frames :54534
# 2 frames :2996
# 3 frames :1071
# 4 frames :581
# 5 frames :307
# 6 frames :1567
# 7 frames :1061
# 8 frames :864
# 9 frames :423
# 10 frames :417
# 11 frames :509
# 12 frames :476
# 13 frames :236
# 14 frames :245
# 15 frames :189
# 16 frames :200
# 17 frames :128
# 18 frames :114
# 19 frames :86
# 20 frames :84
# 21 frames :69
# 22 frames :70
# 23 frames :62
# 24 frames :44
# 25 frames :42
# 26 frames :28
# 27 frames :34
# 28 frames :28
# 29 frames :26
# 30 frames :30
# 31 frames :12
# 32 frames :10
# 33 frames :13
# 34 frames :4
# 35 frames :7
# 36 frames :8
# 37 frames :4
# 38 frames :6
# 39 frames :7
# 40 frames :9
# 41 frames :2
# 42 frames :5
# 43 frames :5
# 44 frames :6
# 45 frames :2
# 46 frames :2
# 47 frames :2
# 48 frames :2
# 50 frames :3
# 54 frames :1
# 57 frames :4
# 58 frames :3
# 59 frames :2
# 69 frames :1
# 78 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.1968
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0