Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-09_20.06.46 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "e7ccdd5ff9285f3d0e20419a96fd7574",
"name": "M1_20170523_1817_dsRNA_H7_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "18:07:06"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8118 (max 8119) in '8118'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.1646
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 7.26895e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 2050 frames Cell Events: 95 mean_circ: 0.951614
Ran for 3050 frames Cell Events: 156 mean_circ: 0.951874
Ran for 3550 frames Cell Events: 182 mean_circ: 0.952033
Ran for 4050 frames Cell Events: 203 mean_circ: 0.952863
Ran for 6050 frames Cell Events: 336 mean_circ: 0.951553
Ran for 6550 frames Cell Events: 361 mean_circ: 0.951903
Ran for 7550 frames Cell Events: 407 mean_circ: 0.951406
Ran for 9050 frames Cell Events: 492 mean_circ: 0.951464
Ran for 10550 frames Cell Events: 548 mean_circ: 0.951865
Ran for 12550 frames Cell Events: 650 mean_circ: 0.949922
Ran for 13050 frames Cell Events: 667 mean_circ: 0.950322
Ran for 14050 frames Cell Events: 725 mean_circ: 0.950764
Ran for 14550 frames Cell Events: 753 mean_circ: 0.951016
Ran for 15550 frames Cell Events: 812 mean_circ: 0.950812
Ran for 16050 frames Cell Events: 836 mean_circ: 0.950883
Ran for 18050 frames Cell Events: 926 mean_circ: 0.951039
Ran for 18550 frames Cell Events: 951 mean_circ: 0.95062
Ran for 19550 frames Cell Events: 1008 mean_circ: 0.950667
Ran for 20050 frames Cell Events: 1031 mean_circ: 0.950726
Ran for 21550 frames Cell Events: 1089 mean_circ: 0.950747
Ran for 22550 frames Cell Events: 1147 mean_circ: 0.950954
Ran for 25050 frames Cell Events: 1270 mean_circ: 0.950967
Ran for 26050 frames Cell Events: 1332 mean_circ: 0.951165
Ran for 26550 frames Cell Events: 1357 mean_circ: 0.951203
Ran for 27550 frames Cell Events: 1395 mean_circ: 0.951259
Ran for 28050 frames Cell Events: 1425 mean_circ: 0.95135
Ran for 28550 frames Cell Events: 1451 mean_circ: 0.951325
Ran for 29550 frames Cell Events: 1519 mean_circ: 0.951325
Ran for 30050 frames Cell Events: 1549 mean_circ: 0.951467
Ran for 30550 frames Cell Events: 1568 mean_circ: 0.951414
Ran for 31050 frames Cell Events: 1599 mean_circ: 0.951417
Ran for 34050 frames Cell Events: 1796 mean_circ: 0.950926
Ran for 35550 frames Cell Events: 1894 mean_circ: 0.950928
Ran for 36050 frames Cell Events: 1910 mean_circ: 0.950671
Ran for 37550 frames Cell Events: 1973 mean_circ: 0.950749
Ran for 38550 frames Cell Events: 2024 mean_circ: 0.950484
Ran for 39050 frames Cell Events: 2039 mean_circ: 0.950513
Ran for 40550 frames Cell Events: 2108 mean_circ: 0.950138
Ran for 41050 frames Cell Events: 2127 mean_circ: 0.950039
Ran for 41550 frames Cell Events: 2145 mean_circ: 0.95011
Ran for 42050 frames Cell Events: 2172 mean_circ: 0.950071
Ran for 44050 frames Cell Events: 2276 mean_circ: 0.950054
Ran for 45050 frames Cell Events: 2320 mean_circ: 0.950063
Ran for 45550 frames Cell Events: 2342 mean_circ: 0.950062
Ran for 47550 frames Cell Events: 2434 mean_circ: 0.950159
Ran for 48050 frames Cell Events: 2456 mean_circ: 0.950281
Ran for 49050 frames Cell Events: 2510 mean_circ: 0.950403
Ran for 49550 frames Cell Events: 2528 mean_circ: 0.950491
Ran for 50550 frames Cell Events: 2594 mean_circ: 0.950538
Ran for 51050 frames Cell Events: 2609 mean_circ: 0.950518
Ran for 52550 frames Cell Events: 2683 mean_circ: 0.950469
Ran for 54050 frames Cell Events: 2778 mean_circ: 0.950401
Ran for 55550 frames Cell Events: 2886 mean_circ: 0.950245
Ran for 56050 frames Cell Events: 2911 mean_circ: 0.950249
Ran for 56550 frames Cell Events: 2933 mean_circ: 0.950163
Ran for 57050 frames Cell Events: 2956 mean_circ: 0.95021
Ran for 57550 frames Cell Events: 2980 mean_circ: 0.950317
Ran for 58050 frames Cell Events: 3005 mean_circ: 0.950408
Ran for 58550 frames Cell Events: 3031 mean_circ: 0.950363
Ran for 59050 frames Cell Events: 3059 mean_circ: 0.95036
Ran for 60550 frames Cell Events: 3120 mean_circ: 0.950364
Ran for 62050 frames Cell Events: 3190 mean_circ: 0.950392
Ran for 62550 frames Cell Events: 3212 mean_circ: 0.950411
Ran for 63550 frames Cell Events: 3254 mean_circ: 0.950388
Ran for 64550 frames Cell Events: 3307 mean_circ: 0.950443
Ran for 68050 frames Cell Events: 3567 mean_circ: 0.950623
Ran for 68550 frames Cell Events: 3603 mean_circ: 0.950681
Ran for 69550 frames Cell Events: 3654 mean_circ: 0.950645
Ran for 70550 frames Cell Events: 3718 mean_circ: 0.950521
Ran for 71550 frames Cell Events: 3773 mean_circ: 0.950533
Ran for 72550 frames Cell Events: 3847 mean_circ: 0.950493
Ran for 73050 frames Cell Events: 3865 mean_circ: 0.950459
Ran for 75050 frames Cell Events: 3956 mean_circ: 0.950377
Ran for 76050 frames Cell Events: 3999 mean_circ: 0.95036
Ran for 76550 frames Cell Events: 4030 mean_circ: 0.950367
Ran for 77050 frames Cell Events: 4062 mean_circ: 0.950417
Ran for 78050 frames Cell Events: 4124 mean_circ: 0.950366
Ran for 79050 frames Cell Events: 4172 mean_circ: 0.950355
Ran for 79550 frames Cell Events: 4196 mean_circ: 0.950355
Ran for 80050 frames Cell Events: 4220 mean_circ: 0.950355
Ran for 81050 frames Cell Events: 4277 mean_circ: 0.950394
Ran for 82050 frames Cell Events: 4323 mean_circ: 0.950351
Ran for 82550 frames Cell Events: 4359 mean_circ: 0.950372
Ran for 83550 frames Cell Events: 4397 mean_circ: 0.950406
Ran for 84050 frames Cell Events: 4422 mean_circ: 0.950402
Ran for 84550 frames Cell Events: 4449 mean_circ: 0.95035
Ran for 85050 frames Cell Events: 4471 mean_circ: 0.950352
Ran for 86050 frames Cell Events: 4525 mean_circ: 0.950374
Ran for 87050 frames Cell Events: 4594 mean_circ: 0.950255
Ran for 88050 frames Cell Events: 4650 mean_circ: 0.950253
Ran for 88550 frames Cell Events: 4674 mean_circ: 0.950133
Ran for 89050 frames Cell Events: 4692 mean_circ: 0.950102
Ran for 89550 frames Cell Events: 4717 mean_circ: 0.950056
Ran for 92050 frames Cell Events: 4843 mean_circ: 0.950202
Ran for 92550 frames Cell Events: 4868 mean_circ: 0.950203
Ran for 93050 frames Cell Events: 4893 mean_circ: 0.950201
Ran for 94050 frames Cell Events: 4973 mean_circ: 0.950209
Ran for 94550 frames Cell Events: 4998 mean_circ: 0.950312
Ran for 95050 frames Cell Events: 5026 mean_circ: 0.950333
Ran for 96550 frames Cell Events: 5105 mean_circ: 0.950205
Ran for 97550 frames Cell Events: 5166 mean_circ: 0.950171
Ran for 98050 frames Cell Events: 5187 mean_circ: 0.95024
Ran for 98550 frames Cell Events: 5209 mean_circ: 0.950278
Ran for 99050 frames Cell Events: 5227 mean_circ: 0.950277
Ran for 100550 frames Cell Events: 5298 mean_circ: 0.950333
Ran for 101050 frames Cell Events: 5319 mean_circ: 0.950322
Ran for 102050 frames Cell Events: 5368 mean_circ: 0.95034
Ran for 103550 frames Cell Events: 5448 mean_circ: 0.950359
Ran for 107550 frames Cell Events: 5693 mean_circ: 0.950316
Ran for 109050 frames Cell Events: 5784 mean_circ: 0.950267
Ran for 111550 frames Cell Events: 5910 mean_circ: 0.95033
Ran for 112050 frames Cell Events: 5938 mean_circ: 0.950378
Ran for 115550 frames Cell Events: 6126 mean_circ: 0.950299
Ran for 116050 frames Cell Events: 6152 mean_circ: 0.950281
Ran for 117050 frames Cell Events: 6219 mean_circ: 0.950337
Ran for 117550 frames Cell Events: 6241 mean_circ: 0.950313
Ran for 120550 frames Cell Events: 6379 mean_circ: 0.950289
Ran for 121050 frames Cell Events: 6398 mean_circ: 0.950272
Ran for 123550 frames Cell Events: 6539 mean_circ: 0.950302
Ran for 124050 frames Cell Events: 6557 mean_circ: 0.950299
Ran for 125050 frames Cell Events: 6612 mean_circ: 0.950317
Ran for 126050 frames Cell Events: 6669 mean_circ: 0.950297
Ran for 127050 frames Cell Events: 6719 mean_circ: 0.950303
Ran for 127550 frames Cell Events: 6740 mean_circ: 0.950274
Ran for 128050 frames Cell Events: 6765 mean_circ: 0.950282
Ran for 129550 frames Cell Events: 6855 mean_circ: 0.950257
Ran for 130550 frames Cell Events: 6902 mean_circ: 0.950252
Ran for 132550 frames Cell Events: 6996 mean_circ: 0.95025
Ran for 133550 frames Cell Events: 7048 mean_circ: 0.950271
Ran for 135550 frames Cell Events: 7175 mean_circ: 0.950291
Ran for 136050 frames Cell Events: 7201 mean_circ: 0.950262
Ran for 136550 frames Cell Events: 7222 mean_circ: 0.950275
Ran for 137550 frames Cell Events: 7294 mean_circ: 0.95027
Ran for 138550 frames Cell Events: 7327 mean_circ: 0.950261
Ran for 139050 frames Cell Events: 7352 mean_circ: 0.950246
Ran for 139550 frames Cell Events: 7377 mean_circ: 0.950273
Ran for 140050 frames Cell Events: 7403 mean_circ: 0.950181
Ran for 140550 frames Cell Events: 7426 mean_circ: 0.950173
Ran for 141050 frames Cell Events: 7441 mean_circ: 0.950165
Ran for 141550 frames Cell Events: 7462 mean_circ: 0.950135
Ran for 143050 frames Cell Events: 7549 mean_circ: 0.950134
Ran for 145050 frames Cell Events: 7647 mean_circ: 0.950083
Ran for 145550 frames Cell Events: 7666 mean_circ: 0.950064
Ran for 146050 frames Cell Events: 7705 mean_circ: 0.950074
Ran for 148050 frames Cell Events: 7806 mean_circ: 0.950096
Ran for 148550 frames Cell Events: 7825 mean_circ: 0.950082
Ran for 149050 frames Cell Events: 7841 mean_circ: 0.950081
Ran for 151050 frames Cell Events: 7953 mean_circ: 0.950051
Ran for 152550 frames Cell Events: 8037 mean_circ: 0.950085
Ran for 153550 frames Cell Events: 8089 mean_circ: 0.950052
Ran for 154050 frames Cell Events: 8105 mean_circ: 0.950062



End this measurment..



Stopping time: 7.26972e+008
****************************************
Measurement finished

Mean Circularitiy: 0.950074
Mean Area: 578.955
Mean FL-1 intensity: 3606.31
Mean FL-2 intensity: 4266.22
Mean FL-3 intensity: 0

frames processed: 151308
Total Time: 77234.4ms
Number of Runs (new bg img) 0
Frames collected: 154327
Skipped frames: 110124
Rescued Frames: 3794995
Samples DAQ: 38582241
Samples written: 77163314
Samples per Channel: 38581657
FPS: 1998.16

Samples per second: 499540
Expected samples/s: 500000

Events: 8119
Events per second: 105.121
jumped back (PMT): 2909
#Frames read, frequency
# 0 frames :243458
# 1 frames :52991
# 2 frames :2759
# 3 frames :1105
# 4 frames :628
# 5 frames :312
# 6 frames :1169
# 7 frames :1020
# 8 frames :851
# 9 frames :581
# 10 frames :635
# 11 frames :527
# 12 frames :367
# 13 frames :240
# 14 frames :205
# 15 frames :186
# 16 frames :186
# 17 frames :150
# 18 frames :143
# 19 frames :116
# 20 frames :98
# 21 frames :77
# 22 frames :71
# 23 frames :74
# 24 frames :65
# 25 frames :51
# 26 frames :61
# 27 frames :29
# 28 frames :31
# 29 frames :34
# 30 frames :29
# 31 frames :19
# 32 frames :31
# 33 frames :23
# 34 frames :25
# 35 frames :29
# 36 frames :24
# 37 frames :4
# 38 frames :9
# 39 frames :6
# 40 frames :6
# 41 frames :10
# 42 frames :8
# 43 frames :7
# 44 frames :4
# 45 frames :5
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# 53 frames :2
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# 57 frames :2
# 58 frames :4
# 59 frames :1
# 60 frames :3
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# 67 frames :2
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M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.1646
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0