Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-09_20.07.13 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "599343ea4f242d9ad84ec8a96e794afb",
"name": "M1_20170523_1820_dsRNA_H7_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "18:07:33"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8068 (max 8069) in '8068'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.1784
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 7.27055e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 37 mean_circ: 0.937581
Ran for 3550 frames Cell Events: 183 mean_circ: 0.941324
Ran for 4550 frames Cell Events: 231 mean_circ: 0.940603
Ran for 5550 frames Cell Events: 302 mean_circ: 0.939609
Ran for 6050 frames Cell Events: 332 mean_circ: 0.938512
Ran for 7550 frames Cell Events: 398 mean_circ: 0.93785
Ran for 8050 frames Cell Events: 419 mean_circ: 0.938167
Ran for 8550 frames Cell Events: 448 mean_circ: 0.937659
Ran for 9050 frames Cell Events: 480 mean_circ: 0.938186
Ran for 9550 frames Cell Events: 510 mean_circ: 0.938313
Ran for 10050 frames Cell Events: 545 mean_circ: 0.938094
Ran for 11550 frames Cell Events: 628 mean_circ: 0.938507
Ran for 12550 frames Cell Events: 688 mean_circ: 0.939348
Ran for 13050 frames Cell Events: 722 mean_circ: 0.939318
Ran for 14550 frames Cell Events: 803 mean_circ: 0.939074
Ran for 16550 frames Cell Events: 880 mean_circ: 0.939283
Ran for 17550 frames Cell Events: 939 mean_circ: 0.939306
Ran for 18550 frames Cell Events: 998 mean_circ: 0.939376
Ran for 19050 frames Cell Events: 1025 mean_circ: 0.939409
Ran for 20050 frames Cell Events: 1061 mean_circ: 0.939383
Ran for 22050 frames Cell Events: 1166 mean_circ: 0.939399
Ran for 23050 frames Cell Events: 1218 mean_circ: 0.939189
Ran for 24550 frames Cell Events: 1304 mean_circ: 0.939334
Ran for 25050 frames Cell Events: 1329 mean_circ: 0.939528
Ran for 26050 frames Cell Events: 1384 mean_circ: 0.939629
Ran for 27050 frames Cell Events: 1442 mean_circ: 0.93983
Ran for 27550 frames Cell Events: 1459 mean_circ: 0.939885
Ran for 28550 frames Cell Events: 1513 mean_circ: 0.939822
Ran for 29550 frames Cell Events: 1569 mean_circ: 0.939756
Ran for 30050 frames Cell Events: 1596 mean_circ: 0.939807
Ran for 31050 frames Cell Events: 1652 mean_circ: 0.93982
Ran for 31550 frames Cell Events: 1682 mean_circ: 0.940016
Ran for 32050 frames Cell Events: 1714 mean_circ: 0.940025
Ran for 33050 frames Cell Events: 1783 mean_circ: 0.939895
Ran for 33550 frames Cell Events: 1813 mean_circ: 0.93979
Ran for 34550 frames Cell Events: 1877 mean_circ: 0.939743
Ran for 35050 frames Cell Events: 1904 mean_circ: 0.939776
Ran for 35550 frames Cell Events: 1924 mean_circ: 0.939869
Ran for 36050 frames Cell Events: 1949 mean_circ: 0.939961
Ran for 36550 frames Cell Events: 1984 mean_circ: 0.939911
Ran for 37050 frames Cell Events: 2010 mean_circ: 0.939979
Ran for 37550 frames Cell Events: 2030 mean_circ: 0.939875
Ran for 38050 frames Cell Events: 2058 mean_circ: 0.939882
Ran for 39050 frames Cell Events: 2108 mean_circ: 0.93997
Ran for 40050 frames Cell Events: 2130 mean_circ: 0.939908
Ran for 40550 frames Cell Events: 2162 mean_circ: 0.939678
Ran for 42050 frames Cell Events: 2238 mean_circ: 0.939813
Ran for 42550 frames Cell Events: 2269 mean_circ: 0.939518
Ran for 43050 frames Cell Events: 2300 mean_circ: 0.939643
Ran for 44050 frames Cell Events: 2365 mean_circ: 0.93978
Ran for 45550 frames Cell Events: 2434 mean_circ: 0.939752
Ran for 47550 frames Cell Events: 2536 mean_circ: 0.939717
Ran for 48050 frames Cell Events: 2576 mean_circ: 0.939782
Ran for 49050 frames Cell Events: 2642 mean_circ: 0.939595
Ran for 49550 frames Cell Events: 2677 mean_circ: 0.939492
Ran for 50050 frames Cell Events: 2701 mean_circ: 0.939562
Ran for 50550 frames Cell Events: 2728 mean_circ: 0.9396
Ran for 51050 frames Cell Events: 2756 mean_circ: 0.93959
Ran for 51550 frames Cell Events: 2782 mean_circ: 0.93966
Ran for 52050 frames Cell Events: 2799 mean_circ: 0.93966
Ran for 53550 frames Cell Events: 2878 mean_circ: 0.939724
Ran for 55550 frames Cell Events: 3022 mean_circ: 0.93895
Ran for 56550 frames Cell Events: 3053 mean_circ: 0.938928
Ran for 57050 frames Cell Events: 3086 mean_circ: 0.938966
Ran for 57550 frames Cell Events: 3109 mean_circ: 0.938988
Ran for 58050 frames Cell Events: 3133 mean_circ: 0.939044
Ran for 60550 frames Cell Events: 3259 mean_circ: 0.938989
Ran for 62550 frames Cell Events: 3326 mean_circ: 0.938955
Ran for 64550 frames Cell Events: 3449 mean_circ: 0.938954
Ran for 65550 frames Cell Events: 3517 mean_circ: 0.938934
Ran for 66050 frames Cell Events: 3544 mean_circ: 0.938972
Ran for 66550 frames Cell Events: 3567 mean_circ: 0.938978
Ran for 67550 frames Cell Events: 3611 mean_circ: 0.938993
Ran for 68050 frames Cell Events: 3643 mean_circ: 0.938981
Ran for 69050 frames Cell Events: 3694 mean_circ: 0.938922
Ran for 69550 frames Cell Events: 3714 mean_circ: 0.938974
Ran for 70550 frames Cell Events: 3767 mean_circ: 0.939096
Ran for 71550 frames Cell Events: 3813 mean_circ: 0.939107
Ran for 72550 frames Cell Events: 3868 mean_circ: 0.939233
Ran for 73550 frames Cell Events: 3927 mean_circ: 0.939168
Ran for 74550 frames Cell Events: 3985 mean_circ: 0.939139
Ran for 75050 frames Cell Events: 4016 mean_circ: 0.939095
Ran for 76050 frames Cell Events: 4068 mean_circ: 0.939098
Ran for 76550 frames Cell Events: 4107 mean_circ: 0.939103
Ran for 77550 frames Cell Events: 4155 mean_circ: 0.939142
Ran for 78550 frames Cell Events: 4210 mean_circ: 0.939152
Ran for 79050 frames Cell Events: 4238 mean_circ: 0.939261
Ran for 79550 frames Cell Events: 4264 mean_circ: 0.939285
Ran for 80050 frames Cell Events: 4290 mean_circ: 0.939343
Ran for 81050 frames Cell Events: 4344 mean_circ: 0.939475
Ran for 81550 frames Cell Events: 4369 mean_circ: 0.939473
Ran for 82050 frames Cell Events: 4387 mean_circ: 0.939462
Ran for 82550 frames Cell Events: 4418 mean_circ: 0.939389
Ran for 84050 frames Cell Events: 4501 mean_circ: 0.939411
Ran for 84550 frames Cell Events: 4523 mean_circ: 0.939422
Ran for 85550 frames Cell Events: 4572 mean_circ: 0.939338
Ran for 86050 frames Cell Events: 4598 mean_circ: 0.939342
Ran for 86550 frames Cell Events: 4613 mean_circ: 0.939281
Ran for 87050 frames Cell Events: 4643 mean_circ: 0.939323
Ran for 87550 frames Cell Events: 4661 mean_circ: 0.939292
Ran for 88050 frames Cell Events: 4680 mean_circ: 0.939314
Ran for 88550 frames Cell Events: 4707 mean_circ: 0.939281
Ran for 89050 frames Cell Events: 4728 mean_circ: 0.939307
Ran for 90550 frames Cell Events: 4805 mean_circ: 0.939402
Ran for 91050 frames Cell Events: 4822 mean_circ: 0.939404
Ran for 91550 frames Cell Events: 4848 mean_circ: 0.939304
Ran for 92550 frames Cell Events: 4898 mean_circ: 0.939346
Ran for 93550 frames Cell Events: 4913 mean_circ: 0.939381
Ran for 94550 frames Cell Events: 4965 mean_circ: 0.939426
Ran for 96050 frames Cell Events: 5043 mean_circ: 0.939533
Ran for 97050 frames Cell Events: 5078 mean_circ: 0.939587
Ran for 97550 frames Cell Events: 5102 mean_circ: 0.939565
Ran for 99050 frames Cell Events: 5208 mean_circ: 0.939496
Ran for 99550 frames Cell Events: 5232 mean_circ: 0.93951
Ran for 100550 frames Cell Events: 5291 mean_circ: 0.939497
Ran for 101050 frames Cell Events: 5326 mean_circ: 0.939488
Ran for 101550 frames Cell Events: 5347 mean_circ: 0.93951
Ran for 103550 frames Cell Events: 5445 mean_circ: 0.939547
Ran for 104050 frames Cell Events: 5476 mean_circ: 0.93963
Ran for 106550 frames Cell Events: 5603 mean_circ: 0.939694
Ran for 107050 frames Cell Events: 5636 mean_circ: 0.939688
Ran for 107550 frames Cell Events: 5666 mean_circ: 0.93962
Ran for 108050 frames Cell Events: 5684 mean_circ: 0.93964
Ran for 109050 frames Cell Events: 5734 mean_circ: 0.939678
Ran for 109550 frames Cell Events: 5768 mean_circ: 0.939642
Ran for 112050 frames Cell Events: 5911 mean_circ: 0.939756
Ran for 112550 frames Cell Events: 5939 mean_circ: 0.939767
Ran for 114550 frames Cell Events: 6054 mean_circ: 0.939789
Ran for 115550 frames Cell Events: 6106 mean_circ: 0.939783
Ran for 116050 frames Cell Events: 6131 mean_circ: 0.939806
Ran for 116550 frames Cell Events: 6158 mean_circ: 0.939792
Ran for 117050 frames Cell Events: 6184 mean_circ: 0.939818
Ran for 117550 frames Cell Events: 6214 mean_circ: 0.939748
Ran for 119050 frames Cell Events: 6286 mean_circ: 0.939691
Ran for 120550 frames Cell Events: 6354 mean_circ: 0.939634
Ran for 121550 frames Cell Events: 6431 mean_circ: 0.939607
Ran for 122050 frames Cell Events: 6459 mean_circ: 0.939441
Ran for 123550 frames Cell Events: 6574 mean_circ: 0.939491
Ran for 124050 frames Cell Events: 6598 mean_circ: 0.939528
Ran for 125550 frames Cell Events: 6681 mean_circ: 0.939572
Ran for 127050 frames Cell Events: 6751 mean_circ: 0.939587
Ran for 129050 frames Cell Events: 6857 mean_circ: 0.93958
Ran for 129550 frames Cell Events: 6882 mean_circ: 0.939612
Ran for 130050 frames Cell Events: 6899 mean_circ: 0.93955
Ran for 131050 frames Cell Events: 6953 mean_circ: 0.939565
Ran for 131550 frames Cell Events: 6994 mean_circ: 0.939593
Ran for 132550 frames Cell Events: 7041 mean_circ: 0.93955
Ran for 133050 frames Cell Events: 7081 mean_circ: 0.939517
Ran for 134050 frames Cell Events: 7135 mean_circ: 0.939542
Ran for 135050 frames Cell Events: 7187 mean_circ: 0.939504
Ran for 136050 frames Cell Events: 7257 mean_circ: 0.939503
Ran for 138550 frames Cell Events: 7389 mean_circ: 0.93956
Ran for 139550 frames Cell Events: 7451 mean_circ: 0.939668
Ran for 140050 frames Cell Events: 7462 mean_circ: 0.939662
Ran for 142050 frames Cell Events: 7566 mean_circ: 0.939622
Ran for 143550 frames Cell Events: 7655 mean_circ: 0.939557
Ran for 144050 frames Cell Events: 7682 mean_circ: 0.939579
Ran for 144550 frames Cell Events: 7708 mean_circ: 0.939579
Ran for 145550 frames Cell Events: 7763 mean_circ: 0.939613
Ran for 146550 frames Cell Events: 7816 mean_circ: 0.939606
Ran for 147050 frames Cell Events: 7844 mean_circ: 0.939584
Ran for 148550 frames Cell Events: 7934 mean_circ: 0.939523
Ran for 150050 frames Cell Events: 8034 mean_circ: 0.939527



End this measurment..



Stopping time: 7.27131e+008
****************************************
Measurement finished

Mean Circularitiy: 0.939542
Mean Area: 576.571
Mean FL-1 intensity: 3494.27
Mean FL-2 intensity: 4456.52
Mean FL-3 intensity: 0

frames processed: 146620
Total Time: 75484.6ms
Number of Runs (new bg img) 0
Frames collected: 150827
Skipped frames: 114331
Rescued Frames: 3871693
Samples DAQ: 37708694
Samples written: 75413284
Samples per Channel: 37706642
FPS: 1998.12

Samples per second: 499527
Expected samples/s: 500000

Events: 8069
Events per second: 106.896
jumped back (PMT): 24
#Frames read, frequency
# 0 frames :231674
# 1 frames :57834
# 2 frames :2913
# 3 frames :985
# 4 frames :524
# 5 frames :219
# 6 frames :1998
# 7 frames :1248
# 8 frames :1021
# 9 frames :515
# 10 frames :381
# 11 frames :297
# 12 frames :295
# 13 frames :273
# 14 frames :243
# 15 frames :167
# 16 frames :145
# 17 frames :130
# 18 frames :70
# 19 frames :62
# 20 frames :74
# 21 frames :66
# 22 frames :48
# 23 frames :60
# 24 frames :50
# 25 frames :36
# 26 frames :33
# 27 frames :31
# 28 frames :23
# 29 frames :17
# 30 frames :20
# 31 frames :21
# 32 frames :13
# 33 frames :11
# 34 frames :19
# 35 frames :7
# 36 frames :8
# 37 frames :4
# 38 frames :9
# 39 frames :6
# 40 frames :4
# 41 frames :5
# 42 frames :5
# 43 frames :3
# 44 frames :1
# 45 frames :4
# 46 frames :5
# 47 frames :7
# 48 frames :2
# 49 frames :4
# 50 frames :6
# 51 frames :1
# 52 frames :1
# 54 frames :2
# 55 frames :1
# 58 frames :2
# 59 frames :2
# 60 frames :3
# 62 frames :2
# 63 frames :1
# 64 frames :1
# 69 frames :1
# 70 frames :1
# 75 frames :1
# 77 frames :1
# 78 frames :1
# 79 frames :1
# 80 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.1784
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0