Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-09_20.09.10 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "38f4a5e83641d2571fe6d6211a3a560d",
"name": "M1_20170523_1852_dsRNA_H2_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "18:09:30"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8115 (max 8116) in '8115'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.215
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 7.28967e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 31 mean_circ: 0.940818
Ran for 1050 frames Cell Events: 62 mean_circ: 0.946089
Ran for 3050 frames Cell Events: 152 mean_circ: 0.946453
Ran for 3550 frames Cell Events: 182 mean_circ: 0.946397
Ran for 4050 frames Cell Events: 214 mean_circ: 0.946626
Ran for 4550 frames Cell Events: 253 mean_circ: 0.945736
Ran for 7050 frames Cell Events: 430 mean_circ: 0.944844
Ran for 9050 frames Cell Events: 538 mean_circ: 0.94311
Ran for 9550 frames Cell Events: 572 mean_circ: 0.942881
Ran for 10550 frames Cell Events: 645 mean_circ: 0.942881
Ran for 12050 frames Cell Events: 758 mean_circ: 0.943344
Ran for 13050 frames Cell Events: 847 mean_circ: 0.943777
Ran for 13550 frames Cell Events: 860 mean_circ: 0.943097
Ran for 14550 frames Cell Events: 925 mean_circ: 0.943162
Ran for 16050 frames Cell Events: 1073 mean_circ: 0.943959
Ran for 16550 frames Cell Events: 1105 mean_circ: 0.943619
Ran for 18050 frames Cell Events: 1212 mean_circ: 0.943316
Ran for 21050 frames Cell Events: 1451 mean_circ: 0.942771
Ran for 21550 frames Cell Events: 1476 mean_circ: 0.942946
Ran for 22050 frames Cell Events: 1512 mean_circ: 0.943088
Ran for 23050 frames Cell Events: 1585 mean_circ: 0.943202
Ran for 24050 frames Cell Events: 1643 mean_circ: 0.943406
Ran for 27550 frames Cell Events: 1875 mean_circ: 0.943347
Ran for 28050 frames Cell Events: 1919 mean_circ: 0.943347
Ran for 30050 frames Cell Events: 2101 mean_circ: 0.943364
Ran for 33050 frames Cell Events: 2376 mean_circ: 0.942767
Ran for 33550 frames Cell Events: 2427 mean_circ: 0.942767
Ran for 36050 frames Cell Events: 2615 mean_circ: 0.942913
Ran for 36550 frames Cell Events: 2649 mean_circ: 0.943005
Ran for 37050 frames Cell Events: 2688 mean_circ: 0.943071
Ran for 40050 frames Cell Events: 2986 mean_circ: 0.943115
Ran for 41050 frames Cell Events: 3056 mean_circ: 0.943031
Ran for 43050 frames Cell Events: 3238 mean_circ: 0.94288
Ran for 44550 frames Cell Events: 3351 mean_circ: 0.9428
Ran for 47550 frames Cell Events: 3610 mean_circ: 0.941925
Ran for 48050 frames Cell Events: 3641 mean_circ: 0.941982
Ran for 49050 frames Cell Events: 3706 mean_circ: 0.941897
Ran for 49550 frames Cell Events: 3745 mean_circ: 0.941802
Ran for 50050 frames Cell Events: 3782 mean_circ: 0.941802
Ran for 50550 frames Cell Events: 3818 mean_circ: 0.941838
Ran for 51550 frames Cell Events: 3866 mean_circ: 0.941894
Ran for 52550 frames Cell Events: 3934 mean_circ: 0.9418
Ran for 58050 frames Cell Events: 4443 mean_circ: 0.941585
Ran for 58550 frames Cell Events: 4468 mean_circ: 0.94157
Ran for 59550 frames Cell Events: 4536 mean_circ: 0.941454
Ran for 60550 frames Cell Events: 4598 mean_circ: 0.941509
Ran for 61050 frames Cell Events: 4634 mean_circ: 0.941545
Ran for 61550 frames Cell Events: 4662 mean_circ: 0.941442
Ran for 62050 frames Cell Events: 4694 mean_circ: 0.941422
Ran for 62550 frames Cell Events: 4731 mean_circ: 0.941475
Ran for 63550 frames Cell Events: 4804 mean_circ: 0.941579
Ran for 65550 frames Cell Events: 4956 mean_circ: 0.94152
Ran for 68050 frames Cell Events: 5111 mean_circ: 0.941425
Ran for 68550 frames Cell Events: 5133 mean_circ: 0.941457
Ran for 69550 frames Cell Events: 5188 mean_circ: 0.94145
Ran for 70550 frames Cell Events: 5275 mean_circ: 0.941563
Ran for 71050 frames Cell Events: 5307 mean_circ: 0.941537
Ran for 72550 frames Cell Events: 5440 mean_circ: 0.941439
Ran for 73550 frames Cell Events: 5496 mean_circ: 0.941402
Ran for 75050 frames Cell Events: 5616 mean_circ: 0.941477
Ran for 76050 frames Cell Events: 5677 mean_circ: 0.941509
Ran for 76550 frames Cell Events: 5711 mean_circ: 0.941513
Ran for 77550 frames Cell Events: 5790 mean_circ: 0.941524
Ran for 78050 frames Cell Events: 5814 mean_circ: 0.941522
Ran for 78550 frames Cell Events: 5840 mean_circ: 0.941514
Ran for 81550 frames Cell Events: 6108 mean_circ: 0.941625
Ran for 82050 frames Cell Events: 6159 mean_circ: 0.941625
Ran for 84050 frames Cell Events: 6321 mean_circ: 0.941576
Ran for 84550 frames Cell Events: 6342 mean_circ: 0.941539
Ran for 85550 frames Cell Events: 6422 mean_circ: 0.941585
Ran for 88050 frames Cell Events: 6640 mean_circ: 0.941533
Ran for 91550 frames Cell Events: 6886 mean_circ: 0.941681
Ran for 92050 frames Cell Events: 6915 mean_circ: 0.941695
Ran for 92550 frames Cell Events: 6937 mean_circ: 0.94164
Ran for 95550 frames Cell Events: 7170 mean_circ: 0.941556
Ran for 96050 frames Cell Events: 7205 mean_circ: 0.941559
Ran for 98050 frames Cell Events: 7299 mean_circ: 0.94155
Ran for 100050 frames Cell Events: 7475 mean_circ: 0.941615
Ran for 102050 frames Cell Events: 7662 mean_circ: 0.941624
Ran for 103550 frames Cell Events: 7807 mean_circ: 0.941523
Ran for 105050 frames Cell Events: 7852 mean_circ: 0.941578
Ran for 105550 frames Cell Events: 7873 mean_circ: 0.941593
Ran for 106050 frames Cell Events: 7885 mean_circ: 0.941593
Ran for 107050 frames Cell Events: 7946 mean_circ: 0.941583
Ran for 108050 frames Cell Events: 8010 mean_circ: 0.941614
Ran for 108550 frames Cell Events: 8053 mean_circ: 0.941603



End this measurment..



Stopping time: 7.29022e+008
****************************************
Measurement finished

Mean Circularitiy: 0.941665
Mean Area: 597.74
Mean FL-1 intensity: 3299.07
Mean FL-2 intensity: 4650.85
Mean FL-3 intensity: 0

frames processed: 105741
Total Time: 54711.3ms
Number of Runs (new bg img) 0
Frames collected: 109253
Skipped frames: 131113
Rescued Frames: 4247544
Samples DAQ: 27313710
Samples written: 54626426
Samples per Channel: 27313213
FPS: 1996.9

Samples per second: 499225
Expected samples/s: 500000

Events: 8116
Events per second: 148.342
jumped back (PMT): 22
#Frames read, frequency
# 0 frames :175228
# 1 frames :33962
# 2 frames :1601
# 3 frames :597
# 4 frames :281
# 5 frames :121
# 6 frames :1755
# 7 frames :827
# 8 frames :699
# 9 frames :419
# 10 frames :252
# 11 frames :203
# 12 frames :156
# 13 frames :177
# 14 frames :225
# 15 frames :165
# 16 frames :122
# 17 frames :102
# 18 frames :66
# 19 frames :82
# 20 frames :70
# 21 frames :68
# 22 frames :67
# 23 frames :61
# 24 frames :44
# 25 frames :32
# 26 frames :36
# 27 frames :38
# 28 frames :38
# 29 frames :33
# 30 frames :17
# 31 frames :21
# 32 frames :15
# 33 frames :16
# 34 frames :13
# 35 frames :18
# 36 frames :19
# 37 frames :11
# 38 frames :13
# 39 frames :5
# 40 frames :5
# 41 frames :4
# 42 frames :4
# 43 frames :4
# 44 frames :3
# 45 frames :4
# 46 frames :4
# 47 frames :4
# 48 frames :4
# 49 frames :3
# 50 frames :2
# 51 frames :3
# 52 frames :3
# 53 frames :3
# 54 frames :2
# 55 frames :6
# 56 frames :2
# 57 frames :4
# 58 frames :1
# 59 frames :3
# 60 frames :2
# 62 frames :1
# 64 frames :1
# 65 frames :1
# 69 frames :1
# 70 frames :1
# 75 frames :1
# 76 frames :1
# 78 frames :1
# 81 frames :1
# 86 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.215
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0