Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_11.30.26 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "3341c1f2920954a60aee4158cca57596",
"name": "M1_20170530_1424_dsRNA_J5_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "09:30:47"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8083 (max 8084) in '8083'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.5878
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.31769e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 33 mean_circ: 0.951112
Ran for 1550 frames Cell Events: 50 mean_circ: 0.946277
Ran for 2050 frames Cell Events: 65 mean_circ: 0.948921
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Ran for 3050 frames Cell Events: 100 mean_circ: 0.950389
Ran for 3550 frames Cell Events: 120 mean_circ: 0.949373
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Ran for 5050 frames Cell Events: 162 mean_circ: 0.948723
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Ran for 7550 frames Cell Events: 256 mean_circ: 0.951268
Ran for 8550 frames Cell Events: 303 mean_circ: 0.949827
Ran for 9550 frames Cell Events: 337 mean_circ: 0.950667
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Ran for 11550 frames Cell Events: 410 mean_circ: 0.951356
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Ran for 12550 frames Cell Events: 434 mean_circ: 0.951481
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Ran for 13550 frames Cell Events: 471 mean_circ: 0.951126
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Ran for 23550 frames Cell Events: 823 mean_circ: 0.949991
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Ran for 67050 frames Cell Events: 2372 mean_circ: 0.949928
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Ran for 70050 frames Cell Events: 2500 mean_circ: 0.949775
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Ran for 73050 frames Cell Events: 2633 mean_circ: 0.949523
Ran for 73550 frames Cell Events: 2647 mean_circ: 0.949433
Ran for 74050 frames Cell Events: 2669 mean_circ: 0.949448
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Ran for 83550 frames Cell Events: 3040 mean_circ: 0.949331
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Ran for 85050 frames Cell Events: 3103 mean_circ: 0.949403
Ran for 85550 frames Cell Events: 3122 mean_circ: 0.949355
Ran for 86050 frames Cell Events: 3134 mean_circ: 0.949341
Ran for 86550 frames Cell Events: 3154 mean_circ: 0.949329
Ran for 87550 frames Cell Events: 3192 mean_circ: 0.949327
Ran for 88050 frames Cell Events: 3216 mean_circ: 0.949292
Ran for 88550 frames Cell Events: 3250 mean_circ: 0.949267
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Ran for 90050 frames Cell Events: 3314 mean_circ: 0.949187
Ran for 90550 frames Cell Events: 3336 mean_circ: 0.94913
Ran for 91050 frames Cell Events: 3358 mean_circ: 0.94916
Ran for 91550 frames Cell Events: 3379 mean_circ: 0.949199
Ran for 92050 frames Cell Events: 3400 mean_circ: 0.949136
Ran for 92550 frames Cell Events: 3425 mean_circ: 0.94909
Ran for 93050 frames Cell Events: 3448 mean_circ: 0.949095
Ran for 94050 frames Cell Events: 3489 mean_circ: 0.94917
Ran for 94550 frames Cell Events: 3505 mean_circ: 0.949136
Ran for 95050 frames Cell Events: 3523 mean_circ: 0.949081
Ran for 95550 frames Cell Events: 3539 mean_circ: 0.949148
Ran for 96550 frames Cell Events: 3581 mean_circ: 0.949254
Ran for 97050 frames Cell Events: 3596 mean_circ: 0.949248
Ran for 98550 frames Cell Events: 3669 mean_circ: 0.949204
Ran for 100550 frames Cell Events: 3759 mean_circ: 0.949296
Ran for 101050 frames Cell Events: 3776 mean_circ: 0.949298
Ran for 101550 frames Cell Events: 3801 mean_circ: 0.949371
Ran for 102050 frames Cell Events: 3819 mean_circ: 0.949402
Ran for 102550 frames Cell Events: 3832 mean_circ: 0.949417
Ran for 103050 frames Cell Events: 3854 mean_circ: 0.949401
Ran for 103550 frames Cell Events: 3881 mean_circ: 0.94943
Ran for 104050 frames Cell Events: 3906 mean_circ: 0.949451
Ran for 105050 frames Cell Events: 3952 mean_circ: 0.949457
Ran for 105550 frames Cell Events: 3969 mean_circ: 0.949375
Ran for 106050 frames Cell Events: 3980 mean_circ: 0.949408
Ran for 106550 frames Cell Events: 3999 mean_circ: 0.949382
Ran for 107550 frames Cell Events: 4039 mean_circ: 0.949427
Ran for 108050 frames Cell Events: 4049 mean_circ: 0.949434
Ran for 109050 frames Cell Events: 4098 mean_circ: 0.949444
Ran for 109550 frames Cell Events: 4127 mean_circ: 0.949518
Ran for 110050 frames Cell Events: 4143 mean_circ: 0.949441
Ran for 110550 frames Cell Events: 4166 mean_circ: 0.949497
Ran for 111050 frames Cell Events: 4190 mean_circ: 0.949554
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Ran for 112050 frames Cell Events: 4227 mean_circ: 0.949541
Ran for 112550 frames Cell Events: 4253 mean_circ: 0.949541
Ran for 113050 frames Cell Events: 4273 mean_circ: 0.949545
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Ran for 115550 frames Cell Events: 4369 mean_circ: 0.949386
Ran for 116050 frames Cell Events: 4390 mean_circ: 0.949402
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Ran for 117050 frames Cell Events: 4434 mean_circ: 0.949427
Ran for 118050 frames Cell Events: 4494 mean_circ: 0.949435
Ran for 118550 frames Cell Events: 4509 mean_circ: 0.949405
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Ran for 121050 frames Cell Events: 4614 mean_circ: 0.949442
Ran for 121550 frames Cell Events: 4655 mean_circ: 0.949404
Ran for 122550 frames Cell Events: 4709 mean_circ: 0.949241
Ran for 123050 frames Cell Events: 4723 mean_circ: 0.949158
Ran for 123550 frames Cell Events: 4746 mean_circ: 0.949165
Ran for 124050 frames Cell Events: 4761 mean_circ: 0.949127
Ran for 124550 frames Cell Events: 4780 mean_circ: 0.94913
Ran for 126050 frames Cell Events: 4845 mean_circ: 0.949213
Ran for 126550 frames Cell Events: 4873 mean_circ: 0.949241
Ran for 127050 frames Cell Events: 4888 mean_circ: 0.949216
Ran for 127550 frames Cell Events: 4906 mean_circ: 0.949261
Ran for 129050 frames Cell Events: 4980 mean_circ: 0.949232
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Ran for 131050 frames Cell Events: 5063 mean_circ: 0.949162
Ran for 131550 frames Cell Events: 5082 mean_circ: 0.949106
Ran for 132050 frames Cell Events: 5111 mean_circ: 0.949094
Ran for 132550 frames Cell Events: 5132 mean_circ: 0.949134
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Ran for 134050 frames Cell Events: 5211 mean_circ: 0.949116
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Ran for 142550 frames Cell Events: 5530 mean_circ: 0.949075
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Ran for 171550 frames Cell Events: 6673 mean_circ: 0.948952
Ran for 172050 frames Cell Events: 6703 mean_circ: 0.948965
Ran for 173050 frames Cell Events: 6744 mean_circ: 0.948993
Ran for 173550 frames Cell Events: 6768 mean_circ: 0.948999
Ran for 174050 frames Cell Events: 6798 mean_circ: 0.948938
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Ran for 177050 frames Cell Events: 6918 mean_circ: 0.948953
Ran for 178050 frames Cell Events: 6954 mean_circ: 0.948943
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Ran for 182550 frames Cell Events: 7135 mean_circ: 0.948895
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Ran for 191050 frames Cell Events: 7426 mean_circ: 0.948796
Ran for 192050 frames Cell Events: 7461 mean_circ: 0.948804
Ran for 192550 frames Cell Events: 7491 mean_circ: 0.948784
Ran for 193550 frames Cell Events: 7528 mean_circ: 0.948829
Ran for 194550 frames Cell Events: 7570 mean_circ: 0.948811
Ran for 195050 frames Cell Events: 7594 mean_circ: 0.948818
Ran for 197050 frames Cell Events: 7672 mean_circ: 0.948835
Ran for 197550 frames Cell Events: 7685 mean_circ: 0.948834
Ran for 198550 frames Cell Events: 7717 mean_circ: 0.948801
Ran for 199550 frames Cell Events: 7771 mean_circ: 0.948791
Ran for 200550 frames Cell Events: 7831 mean_circ: 0.948826
Ran for 201050 frames Cell Events: 7842 mean_circ: 0.948838
Ran for 201550 frames Cell Events: 7857 mean_circ: 0.948857
Ran for 202050 frames Cell Events: 7879 mean_circ: 0.948853
Ran for 203050 frames Cell Events: 7912 mean_circ: 0.948878
Ran for 204050 frames Cell Events: 7954 mean_circ: 0.948885
Ran for 204550 frames Cell Events: 7967 mean_circ: 0.948888
Ran for 205050 frames Cell Events: 7984 mean_circ: 0.948896
Ran for 206050 frames Cell Events: 8022 mean_circ: 0.94885
Ran for 206550 frames Cell Events: 8046 mean_circ: 0.94885
Ran for 207050 frames Cell Events: 8071 mean_circ: 0.948814



End this measurment..



Stopping time: 1.31779e+009
****************************************
Measurement finished

Mean Circularitiy: 0.948831
Mean Area: 568.362
Mean FL-1 intensity: 2403.21
Mean FL-2 intensity: 3139.86
Mean FL-3 intensity: 0

frames processed: 205955
Total Time: 103724ms
Number of Runs (new bg img) 0
Frames collected: 207263
Skipped frames: 13718
Rescued Frames: 705094
Samples DAQ: 51815873
Samples written: 103631530
Samples per Channel: 51815765
FPS: 1998.22

Samples per second: 499555
Expected samples/s: 500000

Events: 8084
Events per second: 77.9378
jumped back (PMT): 5255
#Frames read, frequency
# 0 frames :285394
# 1 frames :113795
# 2 frames :4482
# 3 frames :1423
# 4 frames :574
# 5 frames :250
# 6 frames :2909
# 7 frames :1642
# 8 frames :1150
# 9 frames :578
# 10 frames :438
# 11 frames :390
# 12 frames :342
# 13 frames :201
# 14 frames :241
# 15 frames :151
# 16 frames :104
# 17 frames :77
# 18 frames :50
# 19 frames :41
# 20 frames :51
# 21 frames :40
# 22 frames :29
# 23 frames :31
# 24 frames :21
# 25 frames :14
# 26 frames :8
# 27 frames :11
# 28 frames :8
# 29 frames :9
# 30 frames :4
# 31 frames :5
# 32 frames :6
# 33 frames :7
# 34 frames :3
# 35 frames :2
# 36 frames :2
# 37 frames :3
# 38 frames :1
# 39 frames :1
# 40 frames :3
# 48 frames :1
# 56 frames :1
# 58 frames :2
# 72 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.5878
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0