Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_11.35.02 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "77f95cbd31d703d12aca5d7e4ae09f36",
"name": "M1_20170530_1513_dsRNA_J6_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "09:35:23"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8063 (max 8064) in '8063'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.7771
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.32061e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 23 mean_circ: 0.92791
Ran for 1050 frames Cell Events: 50 mean_circ: 0.93031
Ran for 1550 frames Cell Events: 73 mean_circ: 0.933333
Ran for 2050 frames Cell Events: 102 mean_circ: 0.93677
Ran for 3050 frames Cell Events: 150 mean_circ: 0.939207
Ran for 3550 frames Cell Events: 168 mean_circ: 0.938136
Ran for 4050 frames Cell Events: 187 mean_circ: 0.938205
Ran for 4550 frames Cell Events: 211 mean_circ: 0.939426
Ran for 5050 frames Cell Events: 240 mean_circ: 0.939861
Ran for 6550 frames Cell Events: 340 mean_circ: 0.938144
Ran for 7050 frames Cell Events: 368 mean_circ: 0.937165
Ran for 8050 frames Cell Events: 434 mean_circ: 0.937023
Ran for 8550 frames Cell Events: 463 mean_circ: 0.936213
Ran for 9050 frames Cell Events: 489 mean_circ: 0.935681
Ran for 9550 frames Cell Events: 510 mean_circ: 0.935258
Ran for 10050 frames Cell Events: 535 mean_circ: 0.935657
Ran for 11050 frames Cell Events: 583 mean_circ: 0.935596
Ran for 11550 frames Cell Events: 615 mean_circ: 0.935897
Ran for 13050 frames Cell Events: 694 mean_circ: 0.935079
Ran for 14050 frames Cell Events: 743 mean_circ: 0.934763
Ran for 14550 frames Cell Events: 770 mean_circ: 0.934758
Ran for 15050 frames Cell Events: 801 mean_circ: 0.934799
Ran for 16050 frames Cell Events: 855 mean_circ: 0.935027
Ran for 17050 frames Cell Events: 915 mean_circ: 0.935534
Ran for 18550 frames Cell Events: 1008 mean_circ: 0.936426
Ran for 19050 frames Cell Events: 1031 mean_circ: 0.936135
Ran for 19550 frames Cell Events: 1061 mean_circ: 0.936122
Ran for 20050 frames Cell Events: 1103 mean_circ: 0.93597
Ran for 21550 frames Cell Events: 1193 mean_circ: 0.934826
Ran for 22050 frames Cell Events: 1209 mean_circ: 0.934895
Ran for 23050 frames Cell Events: 1255 mean_circ: 0.935101
Ran for 24050 frames Cell Events: 1312 mean_circ: 0.935525
Ran for 25050 frames Cell Events: 1367 mean_circ: 0.935616
Ran for 25550 frames Cell Events: 1392 mean_circ: 0.935344
Ran for 27050 frames Cell Events: 1484 mean_circ: 0.93525
Ran for 27550 frames Cell Events: 1521 mean_circ: 0.9353
Ran for 28050 frames Cell Events: 1549 mean_circ: 0.935368
Ran for 28550 frames Cell Events: 1572 mean_circ: 0.935308
Ran for 29050 frames Cell Events: 1598 mean_circ: 0.935606
Ran for 30050 frames Cell Events: 1651 mean_circ: 0.935385
Ran for 30550 frames Cell Events: 1681 mean_circ: 0.935364
Ran for 31050 frames Cell Events: 1708 mean_circ: 0.935345
Ran for 32050 frames Cell Events: 1772 mean_circ: 0.935249
Ran for 32550 frames Cell Events: 1793 mean_circ: 0.935416
Ran for 33550 frames Cell Events: 1845 mean_circ: 0.935526
Ran for 34550 frames Cell Events: 1899 mean_circ: 0.935567
Ran for 35050 frames Cell Events: 1919 mean_circ: 0.935416
Ran for 35550 frames Cell Events: 1949 mean_circ: 0.935414
Ran for 36050 frames Cell Events: 1967 mean_circ: 0.935514
Ran for 36550 frames Cell Events: 2000 mean_circ: 0.93561
Ran for 37050 frames Cell Events: 2031 mean_circ: 0.935672
Ran for 39050 frames Cell Events: 2107 mean_circ: 0.935512
Ran for 39550 frames Cell Events: 2153 mean_circ: 0.935518
Ran for 40550 frames Cell Events: 2219 mean_circ: 0.935886
Ran for 41050 frames Cell Events: 2243 mean_circ: 0.935808
Ran for 41550 frames Cell Events: 2281 mean_circ: 0.935631
Ran for 43050 frames Cell Events: 2371 mean_circ: 0.93551
Ran for 43550 frames Cell Events: 2396 mean_circ: 0.935514
Ran for 44550 frames Cell Events: 2444 mean_circ: 0.935388
Ran for 45050 frames Cell Events: 2470 mean_circ: 0.935388
Ran for 45550 frames Cell Events: 2505 mean_circ: 0.935072
Ran for 46050 frames Cell Events: 2538 mean_circ: 0.935078
Ran for 46550 frames Cell Events: 2562 mean_circ: 0.935114
Ran for 47050 frames Cell Events: 2589 mean_circ: 0.935103
Ran for 47550 frames Cell Events: 2621 mean_circ: 0.934982
Ran for 49050 frames Cell Events: 2683 mean_circ: 0.935174
Ran for 49550 frames Cell Events: 2706 mean_circ: 0.9352
Ran for 50050 frames Cell Events: 2741 mean_circ: 0.935155
Ran for 50550 frames Cell Events: 2779 mean_circ: 0.935012
Ran for 51050 frames Cell Events: 2804 mean_circ: 0.934938
Ran for 52050 frames Cell Events: 2850 mean_circ: 0.934861
Ran for 53050 frames Cell Events: 2906 mean_circ: 0.935062
Ran for 53550 frames Cell Events: 2943 mean_circ: 0.935006
Ran for 54550 frames Cell Events: 3006 mean_circ: 0.935042
Ran for 55050 frames Cell Events: 3050 mean_circ: 0.934942
Ran for 55550 frames Cell Events: 3066 mean_circ: 0.935033
Ran for 56050 frames Cell Events: 3105 mean_circ: 0.935051
Ran for 56550 frames Cell Events: 3132 mean_circ: 0.935195
Ran for 57550 frames Cell Events: 3176 mean_circ: 0.935229
Ran for 58050 frames Cell Events: 3204 mean_circ: 0.935209
Ran for 58550 frames Cell Events: 3222 mean_circ: 0.935308
Ran for 59050 frames Cell Events: 3258 mean_circ: 0.935317
Ran for 59550 frames Cell Events: 3272 mean_circ: 0.935342
Ran for 60050 frames Cell Events: 3297 mean_circ: 0.935365
Ran for 60550 frames Cell Events: 3328 mean_circ: 0.935229
Ran for 61550 frames Cell Events: 3376 mean_circ: 0.935322
Ran for 62050 frames Cell Events: 3405 mean_circ: 0.935325
Ran for 63550 frames Cell Events: 3474 mean_circ: 0.935584
Ran for 65050 frames Cell Events: 3547 mean_circ: 0.935537
Ran for 65550 frames Cell Events: 3575 mean_circ: 0.93551
Ran for 66050 frames Cell Events: 3600 mean_circ: 0.935509
Ran for 67050 frames Cell Events: 3645 mean_circ: 0.93542
Ran for 68550 frames Cell Events: 3737 mean_circ: 0.935408
Ran for 69550 frames Cell Events: 3804 mean_circ: 0.935568
Ran for 70050 frames Cell Events: 3832 mean_circ: 0.93559
Ran for 71050 frames Cell Events: 3862 mean_circ: 0.935646
Ran for 71550 frames Cell Events: 3899 mean_circ: 0.935659
Ran for 72050 frames Cell Events: 3927 mean_circ: 0.935731
Ran for 73550 frames Cell Events: 4022 mean_circ: 0.935761
Ran for 74050 frames Cell Events: 4036 mean_circ: 0.935761
Ran for 74550 frames Cell Events: 4060 mean_circ: 0.935791
Ran for 75550 frames Cell Events: 4120 mean_circ: 0.935914
Ran for 77550 frames Cell Events: 4243 mean_circ: 0.93577
Ran for 78550 frames Cell Events: 4309 mean_circ: 0.935841
Ran for 79550 frames Cell Events: 4368 mean_circ: 0.935864
Ran for 80050 frames Cell Events: 4386 mean_circ: 0.935882
Ran for 82550 frames Cell Events: 4552 mean_circ: 0.935988
Ran for 83050 frames Cell Events: 4586 mean_circ: 0.935988
Ran for 83550 frames Cell Events: 4612 mean_circ: 0.935958
Ran for 84050 frames Cell Events: 4630 mean_circ: 0.935947
Ran for 84550 frames Cell Events: 4649 mean_circ: 0.935977
Ran for 86050 frames Cell Events: 4726 mean_circ: 0.936018
Ran for 86550 frames Cell Events: 4754 mean_circ: 0.93604
Ran for 87050 frames Cell Events: 4776 mean_circ: 0.936082
Ran for 88550 frames Cell Events: 4845 mean_circ: 0.936138
Ran for 89550 frames Cell Events: 4888 mean_circ: 0.93615
Ran for 90050 frames Cell Events: 4916 mean_circ: 0.93608
Ran for 91050 frames Cell Events: 4928 mean_circ: 0.936116
Ran for 93050 frames Cell Events: 5045 mean_circ: 0.936154
Ran for 93550 frames Cell Events: 5062 mean_circ: 0.936182
Ran for 94050 frames Cell Events: 5097 mean_circ: 0.936172
Ran for 94550 frames Cell Events: 5130 mean_circ: 0.936133
Ran for 95050 frames Cell Events: 5164 mean_circ: 0.936103
Ran for 95550 frames Cell Events: 5199 mean_circ: 0.936079
Ran for 97550 frames Cell Events: 5301 mean_circ: 0.93618
Ran for 99050 frames Cell Events: 5382 mean_circ: 0.936213
Ran for 102050 frames Cell Events: 5552 mean_circ: 0.935943
Ran for 103550 frames Cell Events: 5645 mean_circ: 0.935849
Ran for 104050 frames Cell Events: 5666 mean_circ: 0.935897
Ran for 104550 frames Cell Events: 5699 mean_circ: 0.935926
Ran for 106050 frames Cell Events: 5797 mean_circ: 0.935947
Ran for 108550 frames Cell Events: 5951 mean_circ: 0.935816
Ran for 109050 frames Cell Events: 5977 mean_circ: 0.935805
Ran for 109550 frames Cell Events: 5997 mean_circ: 0.935815
Ran for 110050 frames Cell Events: 6019 mean_circ: 0.935807
Ran for 111050 frames Cell Events: 6099 mean_circ: 0.935761
Ran for 111550 frames Cell Events: 6133 mean_circ: 0.935744
Ran for 112050 frames Cell Events: 6155 mean_circ: 0.935727
Ran for 113050 frames Cell Events: 6222 mean_circ: 0.93576
Ran for 115050 frames Cell Events: 6327 mean_circ: 0.935722
Ran for 115550 frames Cell Events: 6350 mean_circ: 0.935686
Ran for 116550 frames Cell Events: 6416 mean_circ: 0.935605
Ran for 119050 frames Cell Events: 6553 mean_circ: 0.935602
Ran for 120550 frames Cell Events: 6625 mean_circ: 0.935583
Ran for 121550 frames Cell Events: 6669 mean_circ: 0.935541
Ran for 122050 frames Cell Events: 6700 mean_circ: 0.935585
Ran for 122550 frames Cell Events: 6714 mean_circ: 0.935593
Ran for 123050 frames Cell Events: 6739 mean_circ: 0.935576
Ran for 124550 frames Cell Events: 6820 mean_circ: 0.935672
Ran for 125050 frames Cell Events: 6849 mean_circ: 0.935653
Ran for 126550 frames Cell Events: 6931 mean_circ: 0.935619
Ran for 127050 frames Cell Events: 6958 mean_circ: 0.935621
Ran for 127550 frames Cell Events: 6990 mean_circ: 0.93562
Ran for 128550 frames Cell Events: 7049 mean_circ: 0.935648
Ran for 129050 frames Cell Events: 7067 mean_circ: 0.935685
Ran for 130050 frames Cell Events: 7117 mean_circ: 0.935722
Ran for 131550 frames Cell Events: 7222 mean_circ: 0.93566
Ran for 132050 frames Cell Events: 7255 mean_circ: 0.935655
Ran for 133550 frames Cell Events: 7365 mean_circ: 0.935704
Ran for 134050 frames Cell Events: 7390 mean_circ: 0.935751
Ran for 134550 frames Cell Events: 7424 mean_circ: 0.935751
Ran for 136050 frames Cell Events: 7534 mean_circ: 0.935677
Ran for 136550 frames Cell Events: 7561 mean_circ: 0.93567
Ran for 137050 frames Cell Events: 7589 mean_circ: 0.935632
Ran for 137550 frames Cell Events: 7628 mean_circ: 0.935614
Ran for 138050 frames Cell Events: 7659 mean_circ: 0.935631
Ran for 139550 frames Cell Events: 7735 mean_circ: 0.935657
Ran for 141050 frames Cell Events: 7824 mean_circ: 0.935693
Ran for 143050 frames Cell Events: 7944 mean_circ: 0.935794
Ran for 143550 frames Cell Events: 7955 mean_circ: 0.935801



End this measurment..



Stopping time: 1.32069e+009
****************************************
Measurement finished

Mean Circularitiy: 0.93581
Mean Area: 566.59
Mean FL-1 intensity: 2420.42
Mean FL-2 intensity: 3464.8
Mean FL-3 intensity: 0

frames processed: 142006
Total Time: 72677.2ms
Number of Runs (new bg img) 0
Frames collected: 145166
Skipped frames: 29079
Rescued Frames: 1482184
Samples DAQ: 36291613
Samples written: 72583146
Samples per Channel: 36291573
FPS: 1997.41

Samples per second: 499353
Expected samples/s: 500000

Events: 8064
Events per second: 110.956
jumped back (PMT): 15
#Frames read, frequency
# 0 frames :223759
# 1 frames :54134
# 2 frames :3063
# 3 frames :1018
# 4 frames :569
# 5 frames :261
# 6 frames :1941
# 7 frames :1227
# 8 frames :986
# 9 frames :575
# 10 frames :474
# 11 frames :398
# 12 frames :304
# 13 frames :196
# 14 frames :216
# 15 frames :177
# 16 frames :145
# 17 frames :129
# 18 frames :110
# 19 frames :69
# 20 frames :53
# 21 frames :71
# 22 frames :47
# 23 frames :43
# 24 frames :37
# 25 frames :28
# 26 frames :20
# 27 frames :20
# 28 frames :16
# 29 frames :25
# 30 frames :12
# 31 frames :15
# 32 frames :10
# 33 frames :8
# 34 frames :8
# 35 frames :9
# 36 frames :4
# 37 frames :9
# 38 frames :7
# 39 frames :8
# 40 frames :4
# 41 frames :4
# 42 frames :9
# 43 frames :6
# 44 frames :2
# 45 frames :2
# 46 frames :2
# 47 frames :2
# 48 frames :1
# 49 frames :2
# 50 frames :2
# 51 frames :1
# 52 frames :2
# 54 frames :1
# 57 frames :1
# 58 frames :1
# 62 frames :1
# 69 frames :1
# 88 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.7771
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0