Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_11.37.04 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "49e2074e81b58cecadc654db680d453b",
"name": "M1_20170530_1537_dsRNA_LZ_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "09:37:26"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8076 (max 8077) in '8076'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.8154
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.32209e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 37 mean_circ: 0.930275
Ran for 1550 frames Cell Events: 64 mean_circ: 0.930275
Ran for 2050 frames Cell Events: 82 mean_circ: 0.931849
Ran for 2550 frames Cell Events: 93 mean_circ: 0.933135
Ran for 3550 frames Cell Events: 124 mean_circ: 0.930838
Ran for 5550 frames Cell Events: 211 mean_circ: 0.932395
Ran for 8050 frames Cell Events: 304 mean_circ: 0.931808
Ran for 8550 frames Cell Events: 324 mean_circ: 0.932201
Ran for 9050 frames Cell Events: 349 mean_circ: 0.931652
Ran for 10050 frames Cell Events: 374 mean_circ: 0.932529
Ran for 10550 frames Cell Events: 395 mean_circ: 0.9332
Ran for 11050 frames Cell Events: 409 mean_circ: 0.933301
Ran for 12050 frames Cell Events: 438 mean_circ: 0.93285
Ran for 12550 frames Cell Events: 454 mean_circ: 0.932462
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Ran for 14050 frames Cell Events: 506 mean_circ: 0.932068
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Ran for 54550 frames Cell Events: 1922 mean_circ: 0.931271
Ran for 55050 frames Cell Events: 1937 mean_circ: 0.931138
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Ran for 67050 frames Cell Events: 2340 mean_circ: 0.930117
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Ran for 73550 frames Cell Events: 2569 mean_circ: 0.93001
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Ran for 76050 frames Cell Events: 2649 mean_circ: 0.929981
Ran for 76550 frames Cell Events: 2669 mean_circ: 0.929982
Ran for 77050 frames Cell Events: 2681 mean_circ: 0.929878
Ran for 77550 frames Cell Events: 2699 mean_circ: 0.92999
Ran for 78050 frames Cell Events: 2720 mean_circ: 0.930128
Ran for 78550 frames Cell Events: 2728 mean_circ: 0.930122
Ran for 79050 frames Cell Events: 2755 mean_circ: 0.930115
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Ran for 80050 frames Cell Events: 2808 mean_circ: 0.930118
Ran for 80550 frames Cell Events: 2822 mean_circ: 0.929923
Ran for 81550 frames Cell Events: 2855 mean_circ: 0.929778
Ran for 83050 frames Cell Events: 2915 mean_circ: 0.929903
Ran for 84050 frames Cell Events: 2945 mean_circ: 0.930066
Ran for 84550 frames Cell Events: 2957 mean_circ: 0.929875
Ran for 86050 frames Cell Events: 3013 mean_circ: 0.929986
Ran for 87050 frames Cell Events: 3035 mean_circ: 0.92989
Ran for 87550 frames Cell Events: 3064 mean_circ: 0.929828
Ran for 88050 frames Cell Events: 3091 mean_circ: 0.929786
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Ran for 90050 frames Cell Events: 3183 mean_circ: 0.929697
Ran for 90550 frames Cell Events: 3196 mean_circ: 0.92975
Ran for 91050 frames Cell Events: 3221 mean_circ: 0.929829
Ran for 91550 frames Cell Events: 3244 mean_circ: 0.929836
Ran for 92050 frames Cell Events: 3271 mean_circ: 0.929783
Ran for 92550 frames Cell Events: 3289 mean_circ: 0.929821
Ran for 93050 frames Cell Events: 3311 mean_circ: 0.929856
Ran for 93550 frames Cell Events: 3326 mean_circ: 0.929902
Ran for 94050 frames Cell Events: 3350 mean_circ: 0.929893
Ran for 94550 frames Cell Events: 3381 mean_circ: 0.929927
Ran for 95050 frames Cell Events: 3403 mean_circ: 0.929911
Ran for 95550 frames Cell Events: 3429 mean_circ: 0.929905
Ran for 97050 frames Cell Events: 3494 mean_circ: 0.929869
Ran for 98050 frames Cell Events: 3528 mean_circ: 0.929958
Ran for 99050 frames Cell Events: 3565 mean_circ: 0.929857
Ran for 100550 frames Cell Events: 3628 mean_circ: 0.929846
Ran for 101050 frames Cell Events: 3646 mean_circ: 0.929921
Ran for 101550 frames Cell Events: 3664 mean_circ: 0.929857
Ran for 102550 frames Cell Events: 3709 mean_circ: 0.929765
Ran for 103050 frames Cell Events: 3729 mean_circ: 0.92967
Ran for 105550 frames Cell Events: 3918 mean_circ: 0.929703
Ran for 108050 frames Cell Events: 4009 mean_circ: 0.92955
Ran for 109550 frames Cell Events: 4065 mean_circ: 0.929594
Ran for 110550 frames Cell Events: 4107 mean_circ: 0.929687
Ran for 111050 frames Cell Events: 4133 mean_circ: 0.929694
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Ran for 112050 frames Cell Events: 4183 mean_circ: 0.92942
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Ran for 121550 frames Cell Events: 4574 mean_circ: 0.929464
Ran for 122050 frames Cell Events: 4595 mean_circ: 0.929492
Ran for 122550 frames Cell Events: 4615 mean_circ: 0.929509
Ran for 124050 frames Cell Events: 4661 mean_circ: 0.9296
Ran for 124550 frames Cell Events: 4691 mean_circ: 0.929668
Ran for 125550 frames Cell Events: 4729 mean_circ: 0.929746
Ran for 126550 frames Cell Events: 4767 mean_circ: 0.92973
Ran for 127550 frames Cell Events: 4803 mean_circ: 0.929714
Ran for 128050 frames Cell Events: 4822 mean_circ: 0.929676
Ran for 128550 frames Cell Events: 4844 mean_circ: 0.92971
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Ran for 131050 frames Cell Events: 4946 mean_circ: 0.929871
Ran for 131550 frames Cell Events: 4962 mean_circ: 0.929889
Ran for 132050 frames Cell Events: 4978 mean_circ: 0.929902
Ran for 132550 frames Cell Events: 4992 mean_circ: 0.929793
Ran for 133050 frames Cell Events: 5006 mean_circ: 0.929858
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Ran for 134050 frames Cell Events: 5040 mean_circ: 0.929944
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Ran for 142550 frames Cell Events: 5368 mean_circ: 0.92969
Ran for 143550 frames Cell Events: 5412 mean_circ: 0.929699
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Ran for 147050 frames Cell Events: 5540 mean_circ: 0.92974
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Ran for 174050 frames Cell Events: 6619 mean_circ: 0.928826
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Ran for 196550 frames Cell Events: 7402 mean_circ: 0.928567
Ran for 198050 frames Cell Events: 7474 mean_circ: 0.928606
Ran for 199050 frames Cell Events: 7521 mean_circ: 0.928585
Ran for 199550 frames Cell Events: 7543 mean_circ: 0.928598
Ran for 200050 frames Cell Events: 7555 mean_circ: 0.928581
Ran for 200550 frames Cell Events: 7576 mean_circ: 0.928585
Ran for 201550 frames Cell Events: 7616 mean_circ: 0.928624
Ran for 202050 frames Cell Events: 7635 mean_circ: 0.928634
Ran for 203550 frames Cell Events: 7690 mean_circ: 0.928659
Ran for 204550 frames Cell Events: 7731 mean_circ: 0.928663
Ran for 205550 frames Cell Events: 7765 mean_circ: 0.928609
Ran for 206050 frames Cell Events: 7778 mean_circ: 0.928628
Ran for 206550 frames Cell Events: 7799 mean_circ: 0.928616
Ran for 207050 frames Cell Events: 7821 mean_circ: 0.928591
Ran for 207550 frames Cell Events: 7834 mean_circ: 0.928587
Ran for 208050 frames Cell Events: 7860 mean_circ: 0.928578
Ran for 209050 frames Cell Events: 7902 mean_circ: 0.928579
Ran for 210050 frames Cell Events: 7947 mean_circ: 0.928558
Ran for 210550 frames Cell Events: 7970 mean_circ: 0.928532
Ran for 211050 frames Cell Events: 7988 mean_circ: 0.928514
Ran for 212050 frames Cell Events: 8021 mean_circ: 0.928594
Ran for 212550 frames Cell Events: 8034 mean_circ: 0.928632
Ran for 213550 frames Cell Events: 8058 mean_circ: 0.928619



End this measurment..



Stopping time: 1.3222e+009
****************************************
Measurement finished

Mean Circularitiy: 0.928616
Mean Area: 613.606
Mean FL-1 intensity: 2352.56
Mean FL-2 intensity: 3655.89
Mean FL-3 intensity: 0

frames processed: 210754
Total Time: 107074ms
Number of Runs (new bg img) 0
Frames collected: 213996
Skipped frames: 36663
Rescued Frames: 1805071
Samples DAQ: 53498960
Samples written: 106997782
Samples per Channel: 53498891
FPS: 1998.59

Samples per second: 499645
Expected samples/s: 500000

Events: 8077
Events per second: 75.434
jumped back (PMT): 21
#Frames read, frequency
# 0 frames :301389
# 1 frames :110374
# 2 frames :4633
# 3 frames :1543
# 4 frames :761
# 5 frames :356
# 6 frames :2568
# 7 frames :1710
# 8 frames :1222
# 9 frames :692
# 10 frames :578
# 11 frames :414
# 12 frames :365
# 13 frames :253
# 14 frames :223
# 15 frames :166
# 16 frames :125
# 17 frames :123
# 18 frames :90
# 19 frames :52
# 20 frames :50
# 21 frames :40
# 22 frames :39
# 23 frames :36
# 24 frames :15
# 25 frames :22
# 26 frames :17
# 27 frames :14
# 28 frames :17
# 29 frames :10
# 30 frames :8
# 31 frames :7
# 32 frames :6
# 33 frames :3
# 34 frames :3
# 35 frames :2
# 36 frames :2
# 37 frames :5
# 38 frames :2
# 39 frames :1
# 40 frames :4
# 41 frames :2
# 42 frames :3
# 43 frames :1
# 46 frames :1
# 47 frames :1
# 48 frames :1
# 49 frames :1
# 50 frames :1
# 51 frames :1
# 52 frames :1
# 60 frames :2
# 64 frames :1
# 73 frames :1
# 75 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.8154
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0