Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_11.55.44 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "bbf9dae8f0884b406f2bc680353c403d",
"name": "M1_20170530_2022_c2_dsRNA_A3_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "09:56:06"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8244 (max 8246) in '8244'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.8839
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.3392e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 2050 frames Cell Events: 136 mean_circ: 0.931731
Ran for 2550 frames Cell Events: 158 mean_circ: 0.939237
Ran for 4050 frames Cell Events: 265 mean_circ: 0.944346
Ran for 5550 frames Cell Events: 368 mean_circ: 0.946086
Ran for 6050 frames Cell Events: 394 mean_circ: 0.945597
Ran for 6550 frames Cell Events: 424 mean_circ: 0.945505
Ran for 7050 frames Cell Events: 457 mean_circ: 0.946094
Ran for 8050 frames Cell Events: 511 mean_circ: 0.946374
Ran for 8550 frames Cell Events: 541 mean_circ: 0.946371
Ran for 9050 frames Cell Events: 575 mean_circ: 0.947222
Ran for 10050 frames Cell Events: 663 mean_circ: 0.947169
Ran for 11050 frames Cell Events: 718 mean_circ: 0.947654
Ran for 12050 frames Cell Events: 769 mean_circ: 0.947468
Ran for 13050 frames Cell Events: 824 mean_circ: 0.947841
Ran for 14550 frames Cell Events: 911 mean_circ: 0.947452
Ran for 15050 frames Cell Events: 954 mean_circ: 0.947647
Ran for 17050 frames Cell Events: 1098 mean_circ: 0.94524
Ran for 17550 frames Cell Events: 1123 mean_circ: 0.94469
Ran for 18050 frames Cell Events: 1155 mean_circ: 0.944174
Ran for 19050 frames Cell Events: 1220 mean_circ: 0.943428
Ran for 19550 frames Cell Events: 1257 mean_circ: 0.943191
Ran for 21050 frames Cell Events: 1347 mean_circ: 0.94343
Ran for 22050 frames Cell Events: 1399 mean_circ: 0.942872
Ran for 24050 frames Cell Events: 1534 mean_circ: 0.942694
Ran for 24550 frames Cell Events: 1561 mean_circ: 0.942325
Ran for 25050 frames Cell Events: 1589 mean_circ: 0.942378
Ran for 26050 frames Cell Events: 1640 mean_circ: 0.942212
Ran for 26550 frames Cell Events: 1657 mean_circ: 0.94235
Ran for 27050 frames Cell Events: 1682 mean_circ: 0.942308
Ran for 29050 frames Cell Events: 1846 mean_circ: 0.942274
Ran for 30550 frames Cell Events: 1942 mean_circ: 0.942059
Ran for 31050 frames Cell Events: 1979 mean_circ: 0.942073
Ran for 31550 frames Cell Events: 2011 mean_circ: 0.942378
Ran for 32050 frames Cell Events: 2029 mean_circ: 0.942385
Ran for 33050 frames Cell Events: 2093 mean_circ: 0.94215
Ran for 34550 frames Cell Events: 2144 mean_circ: 0.942272
Ran for 35050 frames Cell Events: 2171 mean_circ: 0.94214
Ran for 35550 frames Cell Events: 2198 mean_circ: 0.942218
Ran for 36050 frames Cell Events: 2215 mean_circ: 0.942324
Ran for 38550 frames Cell Events: 2353 mean_circ: 0.942562
Ran for 39050 frames Cell Events: 2368 mean_circ: 0.942712
Ran for 39550 frames Cell Events: 2393 mean_circ: 0.942649
Ran for 40550 frames Cell Events: 2462 mean_circ: 0.942901
Ran for 43050 frames Cell Events: 2596 mean_circ: 0.943041
Ran for 43550 frames Cell Events: 2617 mean_circ: 0.943167
Ran for 44050 frames Cell Events: 2647 mean_circ: 0.943125
Ran for 44550 frames Cell Events: 2674 mean_circ: 0.943143
Ran for 45050 frames Cell Events: 2712 mean_circ: 0.943291
Ran for 49050 frames Cell Events: 2964 mean_circ: 0.943764
Ran for 51050 frames Cell Events: 3098 mean_circ: 0.943841
Ran for 51550 frames Cell Events: 3129 mean_circ: 0.943882
Ran for 52050 frames Cell Events: 3158 mean_circ: 0.943891
Ran for 52550 frames Cell Events: 3183 mean_circ: 0.944004
Ran for 53550 frames Cell Events: 3247 mean_circ: 0.94387
Ran for 54050 frames Cell Events: 3282 mean_circ: 0.943807
Ran for 55050 frames Cell Events: 3340 mean_circ: 0.943929
Ran for 55550 frames Cell Events: 3367 mean_circ: 0.943934
Ran for 56050 frames Cell Events: 3395 mean_circ: 0.9438
Ran for 58050 frames Cell Events: 3516 mean_circ: 0.9438
Ran for 59050 frames Cell Events: 3580 mean_circ: 0.943985
Ran for 59550 frames Cell Events: 3612 mean_circ: 0.944044
Ran for 60050 frames Cell Events: 3642 mean_circ: 0.944044
Ran for 62050 frames Cell Events: 3792 mean_circ: 0.944
Ran for 67550 frames Cell Events: 4204 mean_circ: 0.944279
Ran for 68550 frames Cell Events: 4262 mean_circ: 0.944371
Ran for 69550 frames Cell Events: 4327 mean_circ: 0.944374
Ran for 70550 frames Cell Events: 4393 mean_circ: 0.944436
Ran for 71550 frames Cell Events: 4447 mean_circ: 0.94449
Ran for 75550 frames Cell Events: 4680 mean_circ: 0.944752
Ran for 76550 frames Cell Events: 4746 mean_circ: 0.944842
Ran for 77050 frames Cell Events: 4769 mean_circ: 0.944574
Ran for 77550 frames Cell Events: 4796 mean_circ: 0.944636
Ran for 78050 frames Cell Events: 4823 mean_circ: 0.944688
Ran for 78550 frames Cell Events: 4852 mean_circ: 0.944755
Ran for 80050 frames Cell Events: 4943 mean_circ: 0.944687
Ran for 80550 frames Cell Events: 4969 mean_circ: 0.944619
Ran for 82550 frames Cell Events: 5104 mean_circ: 0.94454
Ran for 85550 frames Cell Events: 5294 mean_circ: 0.944624
Ran for 86050 frames Cell Events: 5309 mean_circ: 0.944651
Ran for 89550 frames Cell Events: 5521 mean_circ: 0.944626
Ran for 90050 frames Cell Events: 5547 mean_circ: 0.944563
Ran for 92050 frames Cell Events: 5663 mean_circ: 0.944702
Ran for 93050 frames Cell Events: 5734 mean_circ: 0.944615
Ran for 93550 frames Cell Events: 5765 mean_circ: 0.944615
Ran for 94050 frames Cell Events: 5797 mean_circ: 0.944632
Ran for 94550 frames Cell Events: 5824 mean_circ: 0.944689
Ran for 95550 frames Cell Events: 5869 mean_circ: 0.944695
Ran for 97050 frames Cell Events: 5949 mean_circ: 0.944778
Ran for 97550 frames Cell Events: 5973 mean_circ: 0.944766
Ran for 98050 frames Cell Events: 6001 mean_circ: 0.944737
Ran for 98550 frames Cell Events: 6026 mean_circ: 0.94477
Ran for 99050 frames Cell Events: 6063 mean_circ: 0.944803
Ran for 100050 frames Cell Events: 6117 mean_circ: 0.944845
Ran for 102050 frames Cell Events: 6236 mean_circ: 0.944849
Ran for 102550 frames Cell Events: 6269 mean_circ: 0.944773
Ran for 103550 frames Cell Events: 6317 mean_circ: 0.944869
Ran for 106550 frames Cell Events: 6518 mean_circ: 0.944841
Ran for 107050 frames Cell Events: 6546 mean_circ: 0.944847
Ran for 107550 frames Cell Events: 6578 mean_circ: 0.944851
Ran for 108050 frames Cell Events: 6606 mean_circ: 0.944801
Ran for 108550 frames Cell Events: 6627 mean_circ: 0.94479
Ran for 110550 frames Cell Events: 6730 mean_circ: 0.944709
Ran for 111550 frames Cell Events: 6824 mean_circ: 0.944709
Ran for 113050 frames Cell Events: 6933 mean_circ: 0.944698
Ran for 114550 frames Cell Events: 7037 mean_circ: 0.944665
Ran for 115050 frames Cell Events: 7076 mean_circ: 0.944646
Ran for 116550 frames Cell Events: 7165 mean_circ: 0.94467
Ran for 117550 frames Cell Events: 7216 mean_circ: 0.944697
Ran for 118550 frames Cell Events: 7269 mean_circ: 0.944776
Ran for 119050 frames Cell Events: 7303 mean_circ: 0.944804
Ran for 121050 frames Cell Events: 7422 mean_circ: 0.944788
Ran for 122550 frames Cell Events: 7506 mean_circ: 0.94486
Ran for 123050 frames Cell Events: 7535 mean_circ: 0.944936
Ran for 124050 frames Cell Events: 7587 mean_circ: 0.944852
Ran for 124550 frames Cell Events: 7603 mean_circ: 0.944847
Ran for 125550 frames Cell Events: 7653 mean_circ: 0.944794
Ran for 126050 frames Cell Events: 7672 mean_circ: 0.944804
Ran for 126550 frames Cell Events: 7695 mean_circ: 0.944799
Ran for 130050 frames Cell Events: 7937 mean_circ: 0.944896
Ran for 131050 frames Cell Events: 8001 mean_circ: 0.944886
Ran for 131550 frames Cell Events: 8026 mean_circ: 0.944846
Ran for 132550 frames Cell Events: 8068 mean_circ: 0.944908
Ran for 134050 frames Cell Events: 8167 mean_circ: 0.944834
Ran for 135050 frames Cell Events: 8229 mean_circ: 0.944807



End this measurment..



Stopping time: 1.33927e+009
****************************************
Measurement finished

Mean Circularitiy: 0.944802
Mean Area: 591.518
Mean FL-1 intensity: 2891.3
Mean FL-2 intensity: 4005.67
Mean FL-3 intensity: 0

frames processed: 132938
Total Time: 67730.9ms
Number of Runs (new bg img) 0
Frames collected: 135327
Skipped frames: 91295
Rescued Frames: 4429189
Samples DAQ: 33833083
Samples written: 67663220
Samples per Channel: 33831610
FPS: 1998.01

Samples per second: 499501
Expected samples/s: 500000

Events: 8246
Events per second: 121.747
jumped back (PMT): 2972
#Frames read, frequency
# 0 frames :216764
# 1 frames :42281
# 2 frames :2650
# 3 frames :892
# 4 frames :541
# 5 frames :325
# 6 frames :1423
# 7 frames :994
# 8 frames :823
# 9 frames :505
# 10 frames :539
# 11 frames :397
# 12 frames :335
# 13 frames :213
# 14 frames :242
# 15 frames :206
# 16 frames :226
# 17 frames :159
# 18 frames :132
# 19 frames :95
# 20 frames :92
# 21 frames :69
# 22 frames :76
# 23 frames :64
# 24 frames :55
# 25 frames :44
# 26 frames :27
# 27 frames :35
# 28 frames :34
# 29 frames :24
# 30 frames :22
# 31 frames :22
# 32 frames :20
# 33 frames :22
# 34 frames :13
# 35 frames :14
# 36 frames :10
# 37 frames :10
# 38 frames :11
# 39 frames :4
# 40 frames :8
# 41 frames :4
# 42 frames :4
# 43 frames :5
# 44 frames :4
# 45 frames :4
# 46 frames :2
# 47 frames :3
# 48 frames :2
# 49 frames :1
# 50 frames :1
# 51 frames :6
# 52 frames :1
# 53 frames :2
# 54 frames :5
# 55 frames :2
# 56 frames :1
# 58 frames :1
# 59 frames :3
# 64 frames :1
# 65 frames :1
# 66 frames :2
# 69 frames :1
# 70 frames :2
# 78 frames :1
# 80 frames :2
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.8839
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0