Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_11.56.16 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "09fce3b8be8ce5046ee4f72d39c1c5bd",
"name": "M1_20170530_2025_dsRNA_A3_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "09:56:40"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 9193 (max 9194) in '9193'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.9271
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.33938e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 29 mean_circ: 0.920091
Ran for 2550 frames Cell Events: 145 mean_circ: 0.928828
Ran for 3550 frames Cell Events: 209 mean_circ: 0.927192
Ran for 4050 frames Cell Events: 229 mean_circ: 0.929038
Ran for 5550 frames Cell Events: 295 mean_circ: 0.932299
Ran for 6050 frames Cell Events: 311 mean_circ: 0.932874
Ran for 6550 frames Cell Events: 336 mean_circ: 0.933286
Ran for 8050 frames Cell Events: 442 mean_circ: 0.932218
Ran for 8550 frames Cell Events: 469 mean_circ: 0.932695
Ran for 9050 frames Cell Events: 507 mean_circ: 0.932789
Ran for 9550 frames Cell Events: 537 mean_circ: 0.933018
Ran for 10050 frames Cell Events: 584 mean_circ: 0.932339
Ran for 11050 frames Cell Events: 649 mean_circ: 0.932182
Ran for 11550 frames Cell Events: 673 mean_circ: 0.932055
Ran for 12050 frames Cell Events: 722 mean_circ: 0.932112
Ran for 12550 frames Cell Events: 758 mean_circ: 0.933149
Ran for 13550 frames Cell Events: 821 mean_circ: 0.933568
Ran for 14050 frames Cell Events: 844 mean_circ: 0.933794
Ran for 14550 frames Cell Events: 881 mean_circ: 0.93355
Ran for 15550 frames Cell Events: 933 mean_circ: 0.93366
Ran for 16550 frames Cell Events: 1000 mean_circ: 0.93389
Ran for 17050 frames Cell Events: 1017 mean_circ: 0.933854
Ran for 18050 frames Cell Events: 1068 mean_circ: 0.934287
Ran for 20050 frames Cell Events: 1207 mean_circ: 0.934143
Ran for 20550 frames Cell Events: 1236 mean_circ: 0.933966
Ran for 21550 frames Cell Events: 1293 mean_circ: 0.934234
Ran for 24050 frames Cell Events: 1442 mean_circ: 0.933613
Ran for 24550 frames Cell Events: 1469 mean_circ: 0.933499
Ran for 25550 frames Cell Events: 1545 mean_circ: 0.933455
Ran for 26050 frames Cell Events: 1581 mean_circ: 0.933468
Ran for 26550 frames Cell Events: 1613 mean_circ: 0.933385
Ran for 27550 frames Cell Events: 1663 mean_circ: 0.933444
Ran for 28050 frames Cell Events: 1704 mean_circ: 0.933576
Ran for 29550 frames Cell Events: 1820 mean_circ: 0.933521
Ran for 30050 frames Cell Events: 1843 mean_circ: 0.933436
Ran for 30550 frames Cell Events: 1873 mean_circ: 0.933602
Ran for 31050 frames Cell Events: 1900 mean_circ: 0.933422
Ran for 32050 frames Cell Events: 1957 mean_circ: 0.933401
Ran for 33550 frames Cell Events: 2063 mean_circ: 0.933408
Ran for 34550 frames Cell Events: 2122 mean_circ: 0.933342
Ran for 35550 frames Cell Events: 2178 mean_circ: 0.933357
Ran for 37550 frames Cell Events: 2324 mean_circ: 0.93303
Ran for 38050 frames Cell Events: 2351 mean_circ: 0.932932
Ran for 39550 frames Cell Events: 2474 mean_circ: 0.932759
Ran for 40050 frames Cell Events: 2505 mean_circ: 0.932575
Ran for 41050 frames Cell Events: 2588 mean_circ: 0.932777
Ran for 41550 frames Cell Events: 2615 mean_circ: 0.932759
Ran for 42050 frames Cell Events: 2643 mean_circ: 0.932543
Ran for 42550 frames Cell Events: 2682 mean_circ: 0.932617
Ran for 43050 frames Cell Events: 2705 mean_circ: 0.932627
Ran for 43550 frames Cell Events: 2748 mean_circ: 0.932619
Ran for 44050 frames Cell Events: 2784 mean_circ: 0.932767
Ran for 45050 frames Cell Events: 2850 mean_circ: 0.932782
Ran for 46050 frames Cell Events: 2906 mean_circ: 0.9326
Ran for 47550 frames Cell Events: 3006 mean_circ: 0.932819
Ran for 48050 frames Cell Events: 3052 mean_circ: 0.932737
Ran for 51050 frames Cell Events: 3227 mean_circ: 0.932819
Ran for 52050 frames Cell Events: 3301 mean_circ: 0.933049
Ran for 53050 frames Cell Events: 3371 mean_circ: 0.933065
Ran for 53550 frames Cell Events: 3413 mean_circ: 0.933123
Ran for 54050 frames Cell Events: 3458 mean_circ: 0.933161
Ran for 57550 frames Cell Events: 3744 mean_circ: 0.933077
Ran for 59050 frames Cell Events: 3839 mean_circ: 0.933051
Ran for 61050 frames Cell Events: 3986 mean_circ: 0.933321
Ran for 62550 frames Cell Events: 4109 mean_circ: 0.933241
Ran for 63050 frames Cell Events: 4157 mean_circ: 0.933323
Ran for 63550 frames Cell Events: 4181 mean_circ: 0.933303
Ran for 65050 frames Cell Events: 4254 mean_circ: 0.933417
Ran for 66050 frames Cell Events: 4320 mean_circ: 0.933356
Ran for 67050 frames Cell Events: 4388 mean_circ: 0.933235
Ran for 68550 frames Cell Events: 4488 mean_circ: 0.933406
Ran for 70550 frames Cell Events: 4620 mean_circ: 0.933288
Ran for 71050 frames Cell Events: 4648 mean_circ: 0.933379
Ran for 73050 frames Cell Events: 4781 mean_circ: 0.933363
Ran for 74050 frames Cell Events: 4840 mean_circ: 0.933314
Ran for 74550 frames Cell Events: 4866 mean_circ: 0.933348
Ran for 76550 frames Cell Events: 4955 mean_circ: 0.933306
Ran for 77550 frames Cell Events: 5020 mean_circ: 0.933416
Ran for 79050 frames Cell Events: 5100 mean_circ: 0.933354
Ran for 79550 frames Cell Events: 5134 mean_circ: 0.933421
Ran for 80550 frames Cell Events: 5206 mean_circ: 0.933476
Ran for 81550 frames Cell Events: 5276 mean_circ: 0.933374
Ran for 82550 frames Cell Events: 5329 mean_circ: 0.93349
Ran for 84050 frames Cell Events: 5424 mean_circ: 0.933608
Ran for 85050 frames Cell Events: 5491 mean_circ: 0.933557
Ran for 85550 frames Cell Events: 5532 mean_circ: 0.933531
Ran for 86550 frames Cell Events: 5618 mean_circ: 0.933436
Ran for 87050 frames Cell Events: 5645 mean_circ: 0.933452
Ran for 89550 frames Cell Events: 5820 mean_circ: 0.933531
Ran for 90050 frames Cell Events: 5850 mean_circ: 0.933515
Ran for 90550 frames Cell Events: 5871 mean_circ: 0.933519
Ran for 91550 frames Cell Events: 5930 mean_circ: 0.933426
Ran for 93550 frames Cell Events: 6041 mean_circ: 0.933355
Ran for 94050 frames Cell Events: 6071 mean_circ: 0.933288
Ran for 95550 frames Cell Events: 6178 mean_circ: 0.933352
Ran for 96050 frames Cell Events: 6203 mean_circ: 0.933306
Ran for 97550 frames Cell Events: 6316 mean_circ: 0.933009
Ran for 98050 frames Cell Events: 6340 mean_circ: 0.933009
Ran for 99050 frames Cell Events: 6402 mean_circ: 0.93295
Ran for 99550 frames Cell Events: 6420 mean_circ: 0.932969
Ran for 100050 frames Cell Events: 6449 mean_circ: 0.932994
Ran for 100550 frames Cell Events: 6479 mean_circ: 0.933029
Ran for 103050 frames Cell Events: 6653 mean_circ: 0.93299
Ran for 103550 frames Cell Events: 6677 mean_circ: 0.932979
Ran for 104050 frames Cell Events: 6697 mean_circ: 0.932935
Ran for 104550 frames Cell Events: 6719 mean_circ: 0.932952
Ran for 106550 frames Cell Events: 6838 mean_circ: 0.933054
Ran for 108050 frames Cell Events: 6933 mean_circ: 0.933162
Ran for 110050 frames Cell Events: 7077 mean_circ: 0.933095
Ran for 111050 frames Cell Events: 7160 mean_circ: 0.933051
Ran for 112050 frames Cell Events: 7238 mean_circ: 0.933038
Ran for 112550 frames Cell Events: 7264 mean_circ: 0.933068
Ran for 113550 frames Cell Events: 7304 mean_circ: 0.933162
Ran for 114050 frames Cell Events: 7338 mean_circ: 0.93309
Ran for 118050 frames Cell Events: 7625 mean_circ: 0.933092
Ran for 118550 frames Cell Events: 7648 mean_circ: 0.933096
Ran for 121050 frames Cell Events: 7806 mean_circ: 0.933116
Ran for 122550 frames Cell Events: 7887 mean_circ: 0.933238
Ran for 123050 frames Cell Events: 7922 mean_circ: 0.933227
Ran for 123550 frames Cell Events: 7959 mean_circ: 0.933223
Ran for 124050 frames Cell Events: 8002 mean_circ: 0.933241
Ran for 124550 frames Cell Events: 8028 mean_circ: 0.933327
Ran for 125050 frames Cell Events: 8061 mean_circ: 0.93326
Ran for 125550 frames Cell Events: 8093 mean_circ: 0.933266
Ran for 127050 frames Cell Events: 8203 mean_circ: 0.933328
Ran for 128050 frames Cell Events: 8256 mean_circ: 0.933376
Ran for 128550 frames Cell Events: 8274 mean_circ: 0.93337
Ran for 130050 frames Cell Events: 8379 mean_circ: 0.933456
Ran for 131050 frames Cell Events: 8430 mean_circ: 0.933468
Ran for 132550 frames Cell Events: 8526 mean_circ: 0.933428
Ran for 133550 frames Cell Events: 8594 mean_circ: 0.933435
Ran for 134550 frames Cell Events: 8684 mean_circ: 0.933419
Ran for 136050 frames Cell Events: 8773 mean_circ: 0.933328
Ran for 136550 frames Cell Events: 8799 mean_circ: 0.933336
Ran for 137550 frames Cell Events: 8834 mean_circ: 0.933331
Ran for 138050 frames Cell Events: 8854 mean_circ: 0.933338
Ran for 141550 frames Cell Events: 9089 mean_circ: 0.933193
Ran for 142050 frames Cell Events: 9118 mean_circ: 0.933196



End this measurment..



Stopping time: 1.33946e+009
****************************************
Measurement finished

Mean Circularitiy: 0.933128
Mean Area: 589.829
Mean FL-1 intensity: 2825
Mean FL-2 intensity: 4441.91
Mean FL-3 intensity: 0

frames processed: 141289
Total Time: 71585.9ms
Number of Runs (new bg img) 0
Frames collected: 142982
Skipped frames: 92988
Rescued Frames: 4510006
Samples DAQ: 35745943
Samples written: 71490800
Samples per Channel: 35745400
FPS: 1997.35

Samples per second: 499336
Expected samples/s: 500000

Events: 9194
Events per second: 128.433
jumped back (PMT): 25
#Frames read, frequency
# 0 frames :225402
# 1 frames :48583
# 2 frames :2511
# 3 frames :958
# 4 frames :501
# 5 frames :215
# 6 frames :2185
# 7 frames :825
# 8 frames :929
# 9 frames :689
# 10 frames :620
# 11 frames :301
# 12 frames :274
# 13 frames :188
# 14 frames :292
# 15 frames :197
# 16 frames :226
# 17 frames :148
# 18 frames :110
# 19 frames :72
# 20 frames :82
# 21 frames :65
# 22 frames :60
# 23 frames :62
# 24 frames :48
# 25 frames :43
# 26 frames :33
# 27 frames :29
# 28 frames :25
# 29 frames :23
# 30 frames :19
# 31 frames :23
# 32 frames :18
# 33 frames :11
# 34 frames :8
# 35 frames :15
# 36 frames :11
# 37 frames :7
# 38 frames :10
# 39 frames :9
# 40 frames :7
# 41 frames :7
# 42 frames :7
# 43 frames :1
# 44 frames :7
# 45 frames :6
# 46 frames :3
# 47 frames :2
# 48 frames :3
# 49 frames :2
# 50 frames :2
# 51 frames :3
# 52 frames :2
# 55 frames :2
# 56 frames :1
# 57 frames :1
# 58 frames :2
# 61 frames :1
# 62 frames :2
# 63 frames :1
# 65 frames :1
# 71 frames :1
# 73 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.9271
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0