Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_12.02.55 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "b693156f8c00c62ffc30631d993a8784",
"name": "M1_20170603_1459_dsRNA_C3_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "10:03:18"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8123 (max 8124) in '8123'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.0386
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.66537e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 24 mean_circ: 0.937194
Ran for 1550 frames Cell Events: 70 mean_circ: 0.937051
Ran for 2050 frames Cell Events: 98 mean_circ: 0.938536
Ran for 2550 frames Cell Events: 116 mean_circ: 0.938045
Ran for 3050 frames Cell Events: 139 mean_circ: 0.939039
Ran for 4050 frames Cell Events: 212 mean_circ: 0.938947
Ran for 5050 frames Cell Events: 253 mean_circ: 0.938989
Ran for 6050 frames Cell Events: 322 mean_circ: 0.938281
Ran for 6550 frames Cell Events: 335 mean_circ: 0.937737
Ran for 7550 frames Cell Events: 350 mean_circ: 0.938026
Ran for 8050 frames Cell Events: 371 mean_circ: 0.938073
Ran for 8550 frames Cell Events: 392 mean_circ: 0.938302
Ran for 9050 frames Cell Events: 418 mean_circ: 0.938014
Ran for 9550 frames Cell Events: 442 mean_circ: 0.938087
Ran for 10550 frames Cell Events: 493 mean_circ: 0.938997
Ran for 11050 frames Cell Events: 519 mean_circ: 0.93826
Ran for 11550 frames Cell Events: 541 mean_circ: 0.937836
Ran for 13050 frames Cell Events: 619 mean_circ: 0.938151
Ran for 14050 frames Cell Events: 664 mean_circ: 0.938483
Ran for 15050 frames Cell Events: 708 mean_circ: 0.938927
Ran for 15550 frames Cell Events: 727 mean_circ: 0.938887
Ran for 17550 frames Cell Events: 839 mean_circ: 0.93862
Ran for 18050 frames Cell Events: 866 mean_circ: 0.938988
Ran for 18550 frames Cell Events: 894 mean_circ: 0.938934
Ran for 19050 frames Cell Events: 923 mean_circ: 0.938778
Ran for 19550 frames Cell Events: 954 mean_circ: 0.938977
Ran for 20050 frames Cell Events: 970 mean_circ: 0.939047
Ran for 20550 frames Cell Events: 1000 mean_circ: 0.939071
Ran for 21050 frames Cell Events: 1027 mean_circ: 0.939104
Ran for 22550 frames Cell Events: 1095 mean_circ: 0.938679
Ran for 23050 frames Cell Events: 1128 mean_circ: 0.938664
Ran for 23550 frames Cell Events: 1144 mean_circ: 0.938755
Ran for 24050 frames Cell Events: 1163 mean_circ: 0.939091
Ran for 28050 frames Cell Events: 1354 mean_circ: 0.939395
Ran for 29550 frames Cell Events: 1431 mean_circ: 0.939101
Ran for 30050 frames Cell Events: 1456 mean_circ: 0.939037
Ran for 30550 frames Cell Events: 1489 mean_circ: 0.938772
Ran for 31050 frames Cell Events: 1518 mean_circ: 0.938871
Ran for 31550 frames Cell Events: 1547 mean_circ: 0.938851
Ran for 32550 frames Cell Events: 1598 mean_circ: 0.938472
Ran for 33050 frames Cell Events: 1628 mean_circ: 0.938453
Ran for 33550 frames Cell Events: 1652 mean_circ: 0.93849
Ran for 35050 frames Cell Events: 1721 mean_circ: 0.938407
Ran for 36050 frames Cell Events: 1766 mean_circ: 0.938461
Ran for 36550 frames Cell Events: 1790 mean_circ: 0.93849
Ran for 38050 frames Cell Events: 1869 mean_circ: 0.938501
Ran for 38550 frames Cell Events: 1900 mean_circ: 0.938579
Ran for 39550 frames Cell Events: 1958 mean_circ: 0.938889
Ran for 40550 frames Cell Events: 2037 mean_circ: 0.93884
Ran for 41050 frames Cell Events: 2065 mean_circ: 0.93884
Ran for 42550 frames Cell Events: 2164 mean_circ: 0.93904
Ran for 43050 frames Cell Events: 2181 mean_circ: 0.939074
Ran for 43550 frames Cell Events: 2204 mean_circ: 0.939001
Ran for 45050 frames Cell Events: 2295 mean_circ: 0.938602
Ran for 45550 frames Cell Events: 2312 mean_circ: 0.938641
Ran for 47050 frames Cell Events: 2395 mean_circ: 0.938502
Ran for 47550 frames Cell Events: 2430 mean_circ: 0.938695
Ran for 48050 frames Cell Events: 2446 mean_circ: 0.938725
Ran for 49050 frames Cell Events: 2505 mean_circ: 0.938718
Ran for 50050 frames Cell Events: 2546 mean_circ: 0.938718
Ran for 51050 frames Cell Events: 2580 mean_circ: 0.938722
Ran for 51550 frames Cell Events: 2602 mean_circ: 0.938577
Ran for 52050 frames Cell Events: 2625 mean_circ: 0.938605
Ran for 52550 frames Cell Events: 2652 mean_circ: 0.938496
Ran for 54550 frames Cell Events: 2764 mean_circ: 0.938617
Ran for 56050 frames Cell Events: 2839 mean_circ: 0.938739
Ran for 56550 frames Cell Events: 2845 mean_circ: 0.938759
Ran for 57050 frames Cell Events: 2868 mean_circ: 0.938704
Ran for 58050 frames Cell Events: 2919 mean_circ: 0.938548
Ran for 59050 frames Cell Events: 2975 mean_circ: 0.938592
Ran for 60050 frames Cell Events: 3032 mean_circ: 0.938571
Ran for 60550 frames Cell Events: 3062 mean_circ: 0.9385
Ran for 62050 frames Cell Events: 3156 mean_circ: 0.938638
Ran for 66550 frames Cell Events: 3389 mean_circ: 0.938437
Ran for 67050 frames Cell Events: 3418 mean_circ: 0.938529
Ran for 67550 frames Cell Events: 3440 mean_circ: 0.938512
Ran for 68050 frames Cell Events: 3465 mean_circ: 0.938383
Ran for 68550 frames Cell Events: 3486 mean_circ: 0.938456
Ran for 69050 frames Cell Events: 3510 mean_circ: 0.938372
Ran for 69550 frames Cell Events: 3529 mean_circ: 0.938441
Ran for 70550 frames Cell Events: 3562 mean_circ: 0.938453
Ran for 71050 frames Cell Events: 3581 mean_circ: 0.938458
Ran for 71550 frames Cell Events: 3597 mean_circ: 0.938431
Ran for 72050 frames Cell Events: 3615 mean_circ: 0.938383
Ran for 72550 frames Cell Events: 3639 mean_circ: 0.938324
Ran for 73050 frames Cell Events: 3679 mean_circ: 0.938325
Ran for 73550 frames Cell Events: 3707 mean_circ: 0.93829
Ran for 74050 frames Cell Events: 3729 mean_circ: 0.938368
Ran for 75550 frames Cell Events: 3819 mean_circ: 0.938509
Ran for 76550 frames Cell Events: 3864 mean_circ: 0.938451
Ran for 77050 frames Cell Events: 3892 mean_circ: 0.938508
Ran for 78050 frames Cell Events: 3957 mean_circ: 0.938561
Ran for 78550 frames Cell Events: 3987 mean_circ: 0.938615
Ran for 79050 frames Cell Events: 4032 mean_circ: 0.938653
Ran for 79550 frames Cell Events: 4067 mean_circ: 0.938553
Ran for 80050 frames Cell Events: 4092 mean_circ: 0.93851
Ran for 81550 frames Cell Events: 4174 mean_circ: 0.938498
Ran for 82050 frames Cell Events: 4207 mean_circ: 0.938564
Ran for 82550 frames Cell Events: 4228 mean_circ: 0.938588
Ran for 83050 frames Cell Events: 4248 mean_circ: 0.93859
Ran for 84050 frames Cell Events: 4307 mean_circ: 0.938629
Ran for 85050 frames Cell Events: 4359 mean_circ: 0.938651
Ran for 86550 frames Cell Events: 4452 mean_circ: 0.938668
Ran for 87550 frames Cell Events: 4502 mean_circ: 0.938659
Ran for 88550 frames Cell Events: 4554 mean_circ: 0.938603
Ran for 89050 frames Cell Events: 4573 mean_circ: 0.938555
Ran for 90050 frames Cell Events: 4635 mean_circ: 0.938638
Ran for 90550 frames Cell Events: 4664 mean_circ: 0.938688
Ran for 92550 frames Cell Events: 4763 mean_circ: 0.938691
Ran for 94050 frames Cell Events: 4842 mean_circ: 0.938621
Ran for 94550 frames Cell Events: 4875 mean_circ: 0.93858
Ran for 96050 frames Cell Events: 4948 mean_circ: 0.938648
Ran for 96550 frames Cell Events: 4964 mean_circ: 0.938653
Ran for 98050 frames Cell Events: 5040 mean_circ: 0.938629
Ran for 98550 frames Cell Events: 5060 mean_circ: 0.938659
Ran for 99050 frames Cell Events: 5085 mean_circ: 0.938721
Ran for 99550 frames Cell Events: 5109 mean_circ: 0.938782
Ran for 100050 frames Cell Events: 5130 mean_circ: 0.938761
Ran for 100550 frames Cell Events: 5145 mean_circ: 0.938745
Ran for 101050 frames Cell Events: 5162 mean_circ: 0.938782
Ran for 102550 frames Cell Events: 5246 mean_circ: 0.938869
Ran for 104050 frames Cell Events: 5330 mean_circ: 0.938882
Ran for 106050 frames Cell Events: 5445 mean_circ: 0.938908
Ran for 106550 frames Cell Events: 5471 mean_circ: 0.938894
Ran for 107050 frames Cell Events: 5497 mean_circ: 0.938868
Ran for 107550 frames Cell Events: 5528 mean_circ: 0.938862
Ran for 108550 frames Cell Events: 5588 mean_circ: 0.938877
Ran for 109550 frames Cell Events: 5641 mean_circ: 0.938802
Ran for 110050 frames Cell Events: 5684 mean_circ: 0.938786
Ran for 110550 frames Cell Events: 5714 mean_circ: 0.938804
Ran for 111050 frames Cell Events: 5743 mean_circ: 0.938753
Ran for 111550 frames Cell Events: 5762 mean_circ: 0.938736
Ran for 114050 frames Cell Events: 5908 mean_circ: 0.938643
Ran for 116050 frames Cell Events: 5989 mean_circ: 0.938621
Ran for 116550 frames Cell Events: 6008 mean_circ: 0.938626
Ran for 117050 frames Cell Events: 6035 mean_circ: 0.938591
Ran for 118550 frames Cell Events: 6124 mean_circ: 0.938478
Ran for 119050 frames Cell Events: 6142 mean_circ: 0.938494
Ran for 120050 frames Cell Events: 6203 mean_circ: 0.938376
Ran for 123550 frames Cell Events: 6399 mean_circ: 0.938248
Ran for 124050 frames Cell Events: 6418 mean_circ: 0.938193
Ran for 125550 frames Cell Events: 6487 mean_circ: 0.938215
Ran for 126050 frames Cell Events: 6515 mean_circ: 0.938197
Ran for 127050 frames Cell Events: 6577 mean_circ: 0.938213
Ran for 128550 frames Cell Events: 6678 mean_circ: 0.938274
Ran for 130550 frames Cell Events: 6794 mean_circ: 0.938211
Ran for 131550 frames Cell Events: 6848 mean_circ: 0.938281
Ran for 132550 frames Cell Events: 6917 mean_circ: 0.938246
Ran for 133050 frames Cell Events: 6941 mean_circ: 0.938251
Ran for 134050 frames Cell Events: 6999 mean_circ: 0.938183
Ran for 134550 frames Cell Events: 7030 mean_circ: 0.938145
Ran for 136050 frames Cell Events: 7126 mean_circ: 0.938058
Ran for 136550 frames Cell Events: 7147 mean_circ: 0.938052
Ran for 137050 frames Cell Events: 7179 mean_circ: 0.938052
Ran for 139050 frames Cell Events: 7304 mean_circ: 0.938029
Ran for 140050 frames Cell Events: 7355 mean_circ: 0.938045
Ran for 140550 frames Cell Events: 7371 mean_circ: 0.938044
Ran for 141050 frames Cell Events: 7388 mean_circ: 0.938053
Ran for 142550 frames Cell Events: 7466 mean_circ: 0.938029
Ran for 143550 frames Cell Events: 7521 mean_circ: 0.937957
Ran for 144050 frames Cell Events: 7545 mean_circ: 0.937942
Ran for 144550 frames Cell Events: 7585 mean_circ: 0.937938
Ran for 148050 frames Cell Events: 7725 mean_circ: 0.93791
Ran for 149050 frames Cell Events: 7787 mean_circ: 0.937919
Ran for 149550 frames Cell Events: 7811 mean_circ: 0.937906
Ran for 150550 frames Cell Events: 7865 mean_circ: 0.937914
Ran for 152550 frames Cell Events: 7975 mean_circ: 0.937831



End this measurment..



Stopping time: 1.66544e+009
****************************************
Measurement finished

Mean Circularitiy: 0.937666
Mean Area: 581.189
Mean FL-1 intensity: 3349.67
Mean FL-2 intensity: 3522.36
Mean FL-3 intensity: 0

frames processed: 152090
Total Time: 77749.6ms
Number of Runs (new bg img) 0
Frames collected: 155341
Skipped frames: 9225
Rescued Frames: 349422
Samples DAQ: 38835391
Samples written: 77670684
Samples per Channel: 38835342
FPS: 1997.97

Samples per second: 499493
Expected samples/s: 500000

Events: 8124
Events per second: 104.489
jumped back (PMT): 15
#Frames read, frequency
# 0 frames :234938
# 1 frames :62150
# 2 frames :3147
# 3 frames :1250
# 4 frames :603
# 5 frames :264
# 6 frames :2044
# 7 frames :1313
# 8 frames :1129
# 9 frames :672
# 10 frames :579
# 11 frames :403
# 12 frames :286
# 13 frames :198
# 14 frames :218
# 15 frames :197
# 16 frames :152
# 17 frames :135
# 18 frames :70
# 19 frames :65
# 20 frames :56
# 21 frames :40
# 22 frames :58
# 23 frames :33
# 24 frames :37
# 25 frames :25
# 26 frames :18
# 27 frames :12
# 28 frames :19
# 29 frames :13
# 30 frames :8
# 31 frames :8
# 32 frames :12
# 33 frames :9
# 34 frames :5
# 35 frames :7
# 36 frames :3
# 37 frames :6
# 38 frames :2
# 39 frames :3
# 40 frames :1
# 41 frames :2
# 42 frames :3
# 43 frames :1
# 45 frames :1
# 46 frames :5
# 47 frames :3
# 48 frames :1
# 50 frames :2
# 54 frames :1
# 55 frames :1
# 57 frames :1
# 62 frames :2
# 67 frames :1
# 92 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.0386
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0