Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_12.03.25 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "c9b6be7f6a1725688f5031a6c1b332d1",
"name": "M1_20170603_1507_dsRNA_B5_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "10:03:49"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 9377 (max 9379) in '9377'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.0783
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.66589e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 50 frames Cell Events: 2 mean_circ: 0.937664
Ran for 2050 frames Cell Events: 90 mean_circ: 0.940802
Ran for 2550 frames Cell Events: 115 mean_circ: 0.94477
Ran for 3550 frames Cell Events: 188 mean_circ: 0.948394
Ran for 4050 frames Cell Events: 220 mean_circ: 0.948778
Ran for 4550 frames Cell Events: 248 mean_circ: 0.948486
Ran for 5050 frames Cell Events: 287 mean_circ: 0.947719
Ran for 5550 frames Cell Events: 320 mean_circ: 0.947674
Ran for 6050 frames Cell Events: 365 mean_circ: 0.947674
Ran for 6550 frames Cell Events: 397 mean_circ: 0.949312
Ran for 7050 frames Cell Events: 421 mean_circ: 0.949654
Ran for 9050 frames Cell Events: 503 mean_circ: 0.949803
Ran for 10050 frames Cell Events: 562 mean_circ: 0.950101
Ran for 11050 frames Cell Events: 615 mean_circ: 0.949805
Ran for 11550 frames Cell Events: 639 mean_circ: 0.949954
Ran for 12050 frames Cell Events: 658 mean_circ: 0.949832
Ran for 12550 frames Cell Events: 679 mean_circ: 0.950082
Ran for 13050 frames Cell Events: 705 mean_circ: 0.950235
Ran for 14550 frames Cell Events: 785 mean_circ: 0.950824
Ran for 15050 frames Cell Events: 805 mean_circ: 0.951071
Ran for 16050 frames Cell Events: 870 mean_circ: 0.950602
Ran for 17050 frames Cell Events: 917 mean_circ: 0.950688
Ran for 17550 frames Cell Events: 939 mean_circ: 0.950614
Ran for 18550 frames Cell Events: 981 mean_circ: 0.950521
Ran for 20550 frames Cell Events: 1089 mean_circ: 0.950785
Ran for 21050 frames Cell Events: 1121 mean_circ: 0.950681
Ran for 21550 frames Cell Events: 1150 mean_circ: 0.950795
Ran for 22550 frames Cell Events: 1197 mean_circ: 0.95078
Ran for 25050 frames Cell Events: 1323 mean_circ: 0.951116
Ran for 25550 frames Cell Events: 1340 mean_circ: 0.951091
Ran for 28550 frames Cell Events: 1509 mean_circ: 0.95076
Ran for 29550 frames Cell Events: 1545 mean_circ: 0.950509
Ran for 30050 frames Cell Events: 1568 mean_circ: 0.950638
Ran for 30550 frames Cell Events: 1603 mean_circ: 0.950628
Ran for 31550 frames Cell Events: 1662 mean_circ: 0.950431
Ran for 32050 frames Cell Events: 1690 mean_circ: 0.95055
Ran for 33050 frames Cell Events: 1736 mean_circ: 0.950116
Ran for 34050 frames Cell Events: 1786 mean_circ: 0.949978
Ran for 34550 frames Cell Events: 1810 mean_circ: 0.949984
Ran for 35050 frames Cell Events: 1837 mean_circ: 0.949993
Ran for 37050 frames Cell Events: 1909 mean_circ: 0.949928
Ran for 37550 frames Cell Events: 1941 mean_circ: 0.949936
Ran for 38050 frames Cell Events: 1969 mean_circ: 0.949863
Ran for 38550 frames Cell Events: 2001 mean_circ: 0.949926
Ran for 39550 frames Cell Events: 2060 mean_circ: 0.949576
Ran for 41050 frames Cell Events: 2145 mean_circ: 0.949398
Ran for 41550 frames Cell Events: 2167 mean_circ: 0.949418
Ran for 42050 frames Cell Events: 2194 mean_circ: 0.949407
Ran for 43550 frames Cell Events: 2251 mean_circ: 0.949401
Ran for 44050 frames Cell Events: 2295 mean_circ: 0.949285
Ran for 44550 frames Cell Events: 2332 mean_circ: 0.949385
Ran for 45050 frames Cell Events: 2347 mean_circ: 0.949414
Ran for 46050 frames Cell Events: 2404 mean_circ: 0.949395
Ran for 46550 frames Cell Events: 2432 mean_circ: 0.949414
Ran for 48050 frames Cell Events: 2466 mean_circ: 0.949481
Ran for 49050 frames Cell Events: 2527 mean_circ: 0.949486
Ran for 49550 frames Cell Events: 2551 mean_circ: 0.949454
Ran for 50050 frames Cell Events: 2578 mean_circ: 0.949361
Ran for 51550 frames Cell Events: 2666 mean_circ: 0.949396
Ran for 52050 frames Cell Events: 2701 mean_circ: 0.949464
Ran for 52550 frames Cell Events: 2726 mean_circ: 0.949467
Ran for 53050 frames Cell Events: 2751 mean_circ: 0.949478
Ran for 54050 frames Cell Events: 2803 mean_circ: 0.949397
Ran for 55050 frames Cell Events: 2847 mean_circ: 0.949515
Ran for 55550 frames Cell Events: 2878 mean_circ: 0.949529
Ran for 56550 frames Cell Events: 2940 mean_circ: 0.949501
Ran for 57550 frames Cell Events: 3009 mean_circ: 0.949249
Ran for 58050 frames Cell Events: 3042 mean_circ: 0.94924
Ran for 58550 frames Cell Events: 3070 mean_circ: 0.949285
Ran for 61550 frames Cell Events: 3215 mean_circ: 0.949316
Ran for 62050 frames Cell Events: 3244 mean_circ: 0.949371
Ran for 62550 frames Cell Events: 3269 mean_circ: 0.949426
Ran for 64550 frames Cell Events: 3377 mean_circ: 0.949348
Ran for 65050 frames Cell Events: 3398 mean_circ: 0.949343
Ran for 65550 frames Cell Events: 3434 mean_circ: 0.949213
Ran for 66550 frames Cell Events: 3493 mean_circ: 0.949243
Ran for 67050 frames Cell Events: 3519 mean_circ: 0.949241
Ran for 67550 frames Cell Events: 3546 mean_circ: 0.949219
Ran for 68050 frames Cell Events: 3571 mean_circ: 0.949228
Ran for 68550 frames Cell Events: 3594 mean_circ: 0.949189
Ran for 69050 frames Cell Events: 3615 mean_circ: 0.949206
Ran for 70050 frames Cell Events: 3663 mean_circ: 0.94915
Ran for 70550 frames Cell Events: 3677 mean_circ: 0.949195
Ran for 71550 frames Cell Events: 3731 mean_circ: 0.949135
Ran for 72550 frames Cell Events: 3781 mean_circ: 0.94918
Ran for 73050 frames Cell Events: 3811 mean_circ: 0.949185
Ran for 73550 frames Cell Events: 3841 mean_circ: 0.949245
Ran for 74550 frames Cell Events: 3897 mean_circ: 0.949204
Ran for 75550 frames Cell Events: 3934 mean_circ: 0.949217
Ran for 76050 frames Cell Events: 3960 mean_circ: 0.949193
Ran for 77550 frames Cell Events: 4041 mean_circ: 0.949125
Ran for 78050 frames Cell Events: 4062 mean_circ: 0.949133
Ran for 80550 frames Cell Events: 4194 mean_circ: 0.949221
Ran for 81050 frames Cell Events: 4218 mean_circ: 0.949252
Ran for 81550 frames Cell Events: 4257 mean_circ: 0.949182
Ran for 84050 frames Cell Events: 4413 mean_circ: 0.949074
Ran for 84550 frames Cell Events: 4444 mean_circ: 0.949074
Ran for 85550 frames Cell Events: 4507 mean_circ: 0.949029
Ran for 86050 frames Cell Events: 4540 mean_circ: 0.948958
Ran for 86550 frames Cell Events: 4564 mean_circ: 0.948971
Ran for 90050 frames Cell Events: 4750 mean_circ: 0.948903
Ran for 90550 frames Cell Events: 4773 mean_circ: 0.948936
Ran for 91050 frames Cell Events: 4799 mean_circ: 0.948916
Ran for 92050 frames Cell Events: 4848 mean_circ: 0.94883
Ran for 93050 frames Cell Events: 4903 mean_circ: 0.948931
Ran for 94550 frames Cell Events: 4996 mean_circ: 0.948947
Ran for 95550 frames Cell Events: 5049 mean_circ: 0.948926
Ran for 96050 frames Cell Events: 5074 mean_circ: 0.94886
Ran for 96550 frames Cell Events: 5106 mean_circ: 0.948819
Ran for 97050 frames Cell Events: 5142 mean_circ: 0.948807
Ran for 98050 frames Cell Events: 5200 mean_circ: 0.948759
Ran for 98550 frames Cell Events: 5220 mean_circ: 0.948827
Ran for 99050 frames Cell Events: 5236 mean_circ: 0.948759
Ran for 99550 frames Cell Events: 5262 mean_circ: 0.948695
Ran for 100050 frames Cell Events: 5281 mean_circ: 0.948652
Ran for 102050 frames Cell Events: 5391 mean_circ: 0.948648
Ran for 103550 frames Cell Events: 5467 mean_circ: 0.948687
Ran for 104050 frames Cell Events: 5501 mean_circ: 0.94859
Ran for 104550 frames Cell Events: 5526 mean_circ: 0.948637
Ran for 105550 frames Cell Events: 5581 mean_circ: 0.948658
Ran for 106050 frames Cell Events: 5607 mean_circ: 0.948683
Ran for 107050 frames Cell Events: 5660 mean_circ: 0.948733
Ran for 108550 frames Cell Events: 5763 mean_circ: 0.948775
Ran for 110050 frames Cell Events: 5869 mean_circ: 0.948694
Ran for 111050 frames Cell Events: 5928 mean_circ: 0.948719
Ran for 111550 frames Cell Events: 5958 mean_circ: 0.948729
Ran for 112550 frames Cell Events: 6000 mean_circ: 0.94879
Ran for 113550 frames Cell Events: 6050 mean_circ: 0.948778
Ran for 114550 frames Cell Events: 6117 mean_circ: 0.948769
Ran for 115550 frames Cell Events: 6191 mean_circ: 0.94874
Ran for 117050 frames Cell Events: 6277 mean_circ: 0.948749
Ran for 118550 frames Cell Events: 6359 mean_circ: 0.948841
Ran for 120050 frames Cell Events: 6447 mean_circ: 0.948869
Ran for 122050 frames Cell Events: 6604 mean_circ: 0.948913
Ran for 123550 frames Cell Events: 6700 mean_circ: 0.948873
Ran for 125550 frames Cell Events: 6846 mean_circ: 0.948803
Ran for 126050 frames Cell Events: 6875 mean_circ: 0.948779
Ran for 126550 frames Cell Events: 6903 mean_circ: 0.948797
Ran for 127050 frames Cell Events: 6939 mean_circ: 0.948824
Ran for 128050 frames Cell Events: 6990 mean_circ: 0.948831
Ran for 129550 frames Cell Events: 7101 mean_circ: 0.948811
Ran for 130050 frames Cell Events: 7129 mean_circ: 0.948796
Ran for 130550 frames Cell Events: 7162 mean_circ: 0.948815
Ran for 131050 frames Cell Events: 7212 mean_circ: 0.948815
Ran for 131550 frames Cell Events: 7242 mean_circ: 0.948792
Ran for 132550 frames Cell Events: 7301 mean_circ: 0.94873
Ran for 133050 frames Cell Events: 7347 mean_circ: 0.94872
Ran for 135550 frames Cell Events: 7591 mean_circ: 0.948655
Ran for 136550 frames Cell Events: 7654 mean_circ: 0.948762
Ran for 141050 frames Cell Events: 8058 mean_circ: 0.948719
Ran for 143050 frames Cell Events: 8201 mean_circ: 0.948739
Ran for 144050 frames Cell Events: 8274 mean_circ: 0.948653
Ran for 144550 frames Cell Events: 8317 mean_circ: 0.948638
Ran for 145050 frames Cell Events: 8353 mean_circ: 0.948658
Ran for 145550 frames Cell Events: 8387 mean_circ: 0.948676
Ran for 146050 frames Cell Events: 8422 mean_circ: 0.948693
Ran for 148550 frames Cell Events: 8574 mean_circ: 0.948675
Ran for 149050 frames Cell Events: 8601 mean_circ: 0.948684
Ran for 149550 frames Cell Events: 8631 mean_circ: 0.948677
Ran for 151050 frames Cell Events: 8706 mean_circ: 0.948644
Ran for 152050 frames Cell Events: 8767 mean_circ: 0.948646
Ran for 153050 frames Cell Events: 8814 mean_circ: 0.948634
Ran for 154550 frames Cell Events: 8904 mean_circ: 0.948664
Ran for 155550 frames Cell Events: 8976 mean_circ: 0.948676
Ran for 156050 frames Cell Events: 9009 mean_circ: 0.948676
Ran for 158550 frames Cell Events: 9140 mean_circ: 0.948672
Ran for 160050 frames Cell Events: 9193 mean_circ: 0.94868



End this measurment..



Stopping time: 1.66597e+009
****************************************
Measurement finished

Mean Circularitiy: 0.948643
Mean Area: 621.95
Mean FL-1 intensity: 3647.75
Mean FL-2 intensity: 3319.5
Mean FL-3 intensity: 0

frames processed: 159553
Total Time: 81539.8ms
Number of Runs (new bg img) 0
Frames collected: 162900
Skipped frames: 12572
Rescued Frames: 432804
Samples DAQ: 40726750
Samples written: 81450042
Samples per Channel: 40725021
FPS: 1997.8

Samples per second: 499450
Expected samples/s: 500000

Events: 9379
Events per second: 115.024
jumped back (PMT): 4387
#Frames read, frequency
# 0 frames :249553
# 1 frames :62344
# 2 frames :3471
# 3 frames :1111
# 4 frames :618
# 5 frames :297
# 6 frames :2345
# 7 frames :1443
# 8 frames :1154
# 9 frames :590
# 10 frames :487
# 11 frames :277
# 12 frames :269
# 13 frames :221
# 14 frames :289
# 15 frames :220
# 16 frames :166
# 17 frames :141
# 18 frames :79
# 19 frames :75
# 20 frames :87
# 21 frames :61
# 22 frames :76
# 23 frames :58
# 24 frames :34
# 25 frames :22
# 26 frames :24
# 27 frames :33
# 28 frames :22
# 29 frames :22
# 30 frames :14
# 31 frames :18
# 32 frames :13
# 33 frames :11
# 34 frames :9
# 35 frames :11
# 36 frames :7
# 37 frames :10
# 38 frames :6
# 39 frames :7
# 40 frames :5
# 41 frames :1
# 42 frames :3
# 43 frames :5
# 44 frames :3
# 45 frames :5
# 46 frames :3
# 47 frames :3
# 49 frames :1
# 51 frames :1
# 53 frames :3
# 54 frames :3
# 55 frames :1
# 57 frames :1
# 58 frames :2
# 64 frames :1
# 71 frames :1
# 76 frames :1
# 77 frames :1
# 80 frames :1
# 81 frames :1
# 82 frames :1
# 84 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.0783
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0