Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_12.03.58 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "023fd25a60fb022645497abe4d1fb632",
"name": "M1_20170603_1511_dsRNA_B5_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "10:04:22"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 10001 (max 10002) in
'10001'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.0895
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.66609e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 41 mean_circ: 0.934579
Ran for 1050 frames Cell Events: 66 mean_circ: 0.93851
Ran for 2550 frames Cell Events: 152 mean_circ: 0.941071
Ran for 3050 frames Cell Events: 193 mean_circ: 0.941071
Ran for 3550 frames Cell Events: 224 mean_circ: 0.939312
Ran for 8550 frames Cell Events: 575 mean_circ: 0.934791
Ran for 9550 frames Cell Events: 647 mean_circ: 0.934331
Ran for 10550 frames Cell Events: 709 mean_circ: 0.933952
Ran for 11050 frames Cell Events: 734 mean_circ: 0.934019
Ran for 12050 frames Cell Events: 793 mean_circ: 0.934388
Ran for 12550 frames Cell Events: 826 mean_circ: 0.934647
Ran for 13550 frames Cell Events: 909 mean_circ: 0.935094
Ran for 14550 frames Cell Events: 982 mean_circ: 0.935027
Ran for 15550 frames Cell Events: 1059 mean_circ: 0.935616
Ran for 16050 frames Cell Events: 1088 mean_circ: 0.935391
Ran for 18550 frames Cell Events: 1268 mean_circ: 0.935585
Ran for 20050 frames Cell Events: 1373 mean_circ: 0.935548
Ran for 22550 frames Cell Events: 1535 mean_circ: 0.935599
Ran for 24050 frames Cell Events: 1626 mean_circ: 0.935986
Ran for 25050 frames Cell Events: 1685 mean_circ: 0.936063
Ran for 26050 frames Cell Events: 1788 mean_circ: 0.936032
Ran for 29050 frames Cell Events: 1964 mean_circ: 0.93655
Ran for 30550 frames Cell Events: 2052 mean_circ: 0.936431
Ran for 32550 frames Cell Events: 2147 mean_circ: 0.93606
Ran for 33050 frames Cell Events: 2162 mean_circ: 0.936068
Ran for 34550 frames Cell Events: 2290 mean_circ: 0.935712
Ran for 35050 frames Cell Events: 2315 mean_circ: 0.935764
Ran for 35550 frames Cell Events: 2341 mean_circ: 0.935827
Ran for 36050 frames Cell Events: 2378 mean_circ: 0.935788
Ran for 36550 frames Cell Events: 2413 mean_circ: 0.935881
Ran for 40050 frames Cell Events: 2738 mean_circ: 0.935742
Ran for 40550 frames Cell Events: 2767 mean_circ: 0.9357
Ran for 41550 frames Cell Events: 2839 mean_circ: 0.935829
Ran for 42050 frames Cell Events: 2869 mean_circ: 0.935795
Ran for 43050 frames Cell Events: 2916 mean_circ: 0.935798
Ran for 43550 frames Cell Events: 2960 mean_circ: 0.935696
Ran for 44550 frames Cell Events: 3024 mean_circ: 0.935858
Ran for 45550 frames Cell Events: 3091 mean_circ: 0.935698
Ran for 47050 frames Cell Events: 3173 mean_circ: 0.935709
Ran for 47550 frames Cell Events: 3209 mean_circ: 0.935562
Ran for 49050 frames Cell Events: 3324 mean_circ: 0.935694
Ran for 50050 frames Cell Events: 3405 mean_circ: 0.935652
Ran for 50550 frames Cell Events: 3451 mean_circ: 0.935682
Ran for 51050 frames Cell Events: 3494 mean_circ: 0.935763
Ran for 52050 frames Cell Events: 3557 mean_circ: 0.935732
Ran for 53050 frames Cell Events: 3622 mean_circ: 0.935683
Ran for 54550 frames Cell Events: 3723 mean_circ: 0.935494
Ran for 55550 frames Cell Events: 3825 mean_circ: 0.935368
Ran for 56550 frames Cell Events: 3922 mean_circ: 0.935412
Ran for 57050 frames Cell Events: 3959 mean_circ: 0.935412
Ran for 58050 frames Cell Events: 4014 mean_circ: 0.935424
Ran for 61550 frames Cell Events: 4298 mean_circ: 0.935254
Ran for 64550 frames Cell Events: 4562 mean_circ: 0.935194
Ran for 65550 frames Cell Events: 4641 mean_circ: 0.935167
Ran for 69550 frames Cell Events: 4939 mean_circ: 0.935424
Ran for 71550 frames Cell Events: 5108 mean_circ: 0.93535
Ran for 72050 frames Cell Events: 5146 mean_circ: 0.935374
Ran for 72550 frames Cell Events: 5175 mean_circ: 0.935392
Ran for 74050 frames Cell Events: 5271 mean_circ: 0.935442
Ran for 74550 frames Cell Events: 5290 mean_circ: 0.935455
Ran for 75050 frames Cell Events: 5334 mean_circ: 0.935455
Ran for 75550 frames Cell Events: 5376 mean_circ: 0.935377
Ran for 76550 frames Cell Events: 5454 mean_circ: 0.935313
Ran for 77050 frames Cell Events: 5486 mean_circ: 0.935353
Ran for 77550 frames Cell Events: 5525 mean_circ: 0.935324
Ran for 78550 frames Cell Events: 5599 mean_circ: 0.935347
Ran for 79050 frames Cell Events: 5628 mean_circ: 0.935287
Ran for 80050 frames Cell Events: 5693 mean_circ: 0.93522
Ran for 80550 frames Cell Events: 5727 mean_circ: 0.93524
Ran for 82550 frames Cell Events: 5890 mean_circ: 0.935209
Ran for 83050 frames Cell Events: 5932 mean_circ: 0.935137
Ran for 83550 frames Cell Events: 5969 mean_circ: 0.935186
Ran for 85050 frames Cell Events: 6074 mean_circ: 0.93519
Ran for 86050 frames Cell Events: 6124 mean_circ: 0.935168
Ran for 87050 frames Cell Events: 6188 mean_circ: 0.935098
Ran for 87550 frames Cell Events: 6222 mean_circ: 0.935116
Ran for 89550 frames Cell Events: 6381 mean_circ: 0.935066
Ran for 90050 frames Cell Events: 6426 mean_circ: 0.935044
Ran for 91550 frames Cell Events: 6541 mean_circ: 0.935054
Ran for 95550 frames Cell Events: 6864 mean_circ: 0.935186
Ran for 96550 frames Cell Events: 6929 mean_circ: 0.935149
Ran for 97050 frames Cell Events: 6952 mean_circ: 0.935179
Ran for 97550 frames Cell Events: 6993 mean_circ: 0.935155
Ran for 98550 frames Cell Events: 7052 mean_circ: 0.935113
Ran for 99050 frames Cell Events: 7080 mean_circ: 0.935122
Ran for 99550 frames Cell Events: 7112 mean_circ: 0.935096
Ran for 102550 frames Cell Events: 7358 mean_circ: 0.935151
Ran for 103550 frames Cell Events: 7437 mean_circ: 0.935144
Ran for 104550 frames Cell Events: 7511 mean_circ: 0.935174
Ran for 105050 frames Cell Events: 7539 mean_circ: 0.93514
Ran for 106550 frames Cell Events: 7650 mean_circ: 0.935106
Ran for 108050 frames Cell Events: 7753 mean_circ: 0.935027
Ran for 110550 frames Cell Events: 7916 mean_circ: 0.935009
Ran for 111550 frames Cell Events: 7995 mean_circ: 0.935058
Ran for 112550 frames Cell Events: 8076 mean_circ: 0.934959
Ran for 113050 frames Cell Events: 8107 mean_circ: 0.934978
Ran for 114050 frames Cell Events: 8189 mean_circ: 0.934797
Ran for 114550 frames Cell Events: 8224 mean_circ: 0.934746
Ran for 116550 frames Cell Events: 8391 mean_circ: 0.934798
Ran for 117050 frames Cell Events: 8433 mean_circ: 0.934783
Ran for 117550 frames Cell Events: 8465 mean_circ: 0.934773
Ran for 118550 frames Cell Events: 8511 mean_circ: 0.93479
Ran for 119550 frames Cell Events: 8578 mean_circ: 0.93473
Ran for 120550 frames Cell Events: 8661 mean_circ: 0.934729
Ran for 122050 frames Cell Events: 8750 mean_circ: 0.93464
Ran for 122550 frames Cell Events: 8788 mean_circ: 0.934678
Ran for 123550 frames Cell Events: 8868 mean_circ: 0.934683
Ran for 126050 frames Cell Events: 9016 mean_circ: 0.93476
Ran for 126550 frames Cell Events: 9042 mean_circ: 0.934769
Ran for 127050 frames Cell Events: 9065 mean_circ: 0.934767
Ran for 128050 frames Cell Events: 9154 mean_circ: 0.934755
Ran for 128550 frames Cell Events: 9179 mean_circ: 0.934763
Ran for 130050 frames Cell Events: 9254 mean_circ: 0.934746
Ran for 130550 frames Cell Events: 9286 mean_circ: 0.934762
Ran for 131550 frames Cell Events: 9348 mean_circ: 0.934779
Ran for 132050 frames Cell Events: 9366 mean_circ: 0.9348
Ran for 134050 frames Cell Events: 9527 mean_circ: 0.934842
Ran for 135050 frames Cell Events: 9612 mean_circ: 0.934858
Ran for 135550 frames Cell Events: 9652 mean_circ: 0.934915
Ran for 136050 frames Cell Events: 9681 mean_circ: 0.934902
Ran for 137550 frames Cell Events: 9819 mean_circ: 0.934868
Ran for 138050 frames Cell Events: 9842 mean_circ: 0.934851
Ran for 138550 frames Cell Events: 9886 mean_circ: 0.934827
Ran for 139550 frames Cell Events: 9954 mean_circ: 0.934836



End this measurment.
because stop condition (time/nr of cells) was met

Stopping time: 1.66616e+009
****************************************
Measurement finished

Mean Circularitiy: 0.934816
Mean Area: 622.825
Mean FL-1 intensity: 3462.87
Mean FL-2 intensity: 3522.46
Mean FL-3 intensity: 0

frames processed: 137524
Total Time: 70122.5ms
Number of Runs (new bg img) 0
Frames collected: 140110
Skipped frames: 15158
Rescued Frames: 516636
Samples DAQ: 35030369
Samples written: 70055036
Samples per Channel: 35027518
FPS: 1998.07

Samples per second: 499519
Expected samples/s: 500000

Events: 10002
Events per second: 142.636
jumped back (PMT): 35
#Frames read, frequency
# 0 frames :225442
# 1 frames :41817
# 2 frames :2340
# 3 frames :942
# 4 frames :454
# 5 frames :221
# 6 frames :2299
# 7 frames :1114
# 8 frames :957
# 9 frames :549
# 10 frames :403
# 11 frames :291
# 12 frames :252
# 13 frames :199
# 14 frames :313
# 15 frames :233
# 16 frames :186
# 17 frames :135
# 18 frames :99
# 19 frames :97
# 20 frames :101
# 21 frames :88
# 22 frames :70
# 23 frames :62
# 24 frames :49
# 25 frames :50
# 26 frames :42
# 27 frames :44
# 28 frames :32
# 29 frames :22
# 30 frames :33
# 31 frames :28
# 32 frames :18
# 33 frames :19
# 34 frames :9
# 35 frames :15
# 36 frames :6
# 37 frames :15
# 38 frames :16
# 39 frames :9
# 40 frames :10
# 41 frames :10
# 42 frames :5
# 43 frames :8
# 44 frames :2
# 45 frames :4
# 46 frames :6
# 47 frames :6
# 48 frames :2
# 49 frames :6
# 50 frames :4
# 51 frames :3
# 52 frames :6
# 53 frames :2
# 55 frames :6
# 56 frames :2
# 57 frames :1
# 60 frames :1
# 62 frames :1
# 65 frames :1
# 67 frames :1
# 68 frames :1
# 69 frames :2
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.0895
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0