Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_12.04.57 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "00f118dbb5e1992c0b615186b80ce01a",
"name": "M1_20170603_1521_dsRNA_M1_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "10:05:17"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8063 (max 8064) in '8063'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.1534
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.66672e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 62 mean_circ: 0.923809
Ran for 1550 frames Cell Events: 82 mean_circ: 0.915366
Ran for 2050 frames Cell Events: 105 mean_circ: 0.915767
Ran for 3050 frames Cell Events: 158 mean_circ: 0.913056
Ran for 4550 frames Cell Events: 241 mean_circ: 0.912476
Ran for 6550 frames Cell Events: 321 mean_circ: 0.915506
Ran for 7050 frames Cell Events: 343 mean_circ: 0.916683
Ran for 10050 frames Cell Events: 493 mean_circ: 0.916703
Ran for 10550 frames Cell Events: 518 mean_circ: 0.916966
Ran for 11050 frames Cell Events: 540 mean_circ: 0.917249
Ran for 13050 frames Cell Events: 646 mean_circ: 0.917754
Ran for 13550 frames Cell Events: 668 mean_circ: 0.917534
Ran for 14050 frames Cell Events: 698 mean_circ: 0.917087
Ran for 15050 frames Cell Events: 751 mean_circ: 0.916697
Ran for 16050 frames Cell Events: 799 mean_circ: 0.916935
Ran for 18050 frames Cell Events: 907 mean_circ: 0.917106
Ran for 19550 frames Cell Events: 991 mean_circ: 0.916864
Ran for 20550 frames Cell Events: 1045 mean_circ: 0.917255
Ran for 21550 frames Cell Events: 1097 mean_circ: 0.917015
Ran for 22050 frames Cell Events: 1126 mean_circ: 0.917075
Ran for 22550 frames Cell Events: 1157 mean_circ: 0.917223
Ran for 23550 frames Cell Events: 1207 mean_circ: 0.917038
Ran for 24050 frames Cell Events: 1225 mean_circ: 0.916804
Ran for 25550 frames Cell Events: 1307 mean_circ: 0.917033
Ran for 27050 frames Cell Events: 1393 mean_circ: 0.917071
Ran for 28050 frames Cell Events: 1452 mean_circ: 0.916919
Ran for 28550 frames Cell Events: 1476 mean_circ: 0.917063
Ran for 29550 frames Cell Events: 1526 mean_circ: 0.917397
Ran for 32050 frames Cell Events: 1619 mean_circ: 0.917896
Ran for 32550 frames Cell Events: 1637 mean_circ: 0.917416
Ran for 33550 frames Cell Events: 1673 mean_circ: 0.917167
Ran for 34050 frames Cell Events: 1704 mean_circ: 0.917302
Ran for 34550 frames Cell Events: 1724 mean_circ: 0.917378
Ran for 35550 frames Cell Events: 1783 mean_circ: 0.917243
Ran for 36550 frames Cell Events: 1831 mean_circ: 0.917393
Ran for 37050 frames Cell Events: 1853 mean_circ: 0.917343
Ran for 37550 frames Cell Events: 1887 mean_circ: 0.917477
Ran for 38050 frames Cell Events: 1916 mean_circ: 0.917567
Ran for 38550 frames Cell Events: 1930 mean_circ: 0.917528
Ran for 39550 frames Cell Events: 1978 mean_circ: 0.917351
Ran for 40050 frames Cell Events: 2006 mean_circ: 0.917419
Ran for 41050 frames Cell Events: 2062 mean_circ: 0.917273
Ran for 41550 frames Cell Events: 2085 mean_circ: 0.917301
Ran for 42050 frames Cell Events: 2117 mean_circ: 0.917197
Ran for 42550 frames Cell Events: 2142 mean_circ: 0.917334
Ran for 43050 frames Cell Events: 2173 mean_circ: 0.917334
Ran for 43550 frames Cell Events: 2195 mean_circ: 0.917364
Ran for 44050 frames Cell Events: 2217 mean_circ: 0.917455
Ran for 48050 frames Cell Events: 2407 mean_circ: 0.917075
Ran for 48550 frames Cell Events: 2425 mean_circ: 0.917004
Ran for 51550 frames Cell Events: 2570 mean_circ: 0.916733
Ran for 52050 frames Cell Events: 2584 mean_circ: 0.916584
Ran for 53050 frames Cell Events: 2638 mean_circ: 0.916587
Ran for 54050 frames Cell Events: 2685 mean_circ: 0.916295
Ran for 54550 frames Cell Events: 2700 mean_circ: 0.916267
Ran for 55550 frames Cell Events: 2766 mean_circ: 0.916442
Ran for 57550 frames Cell Events: 2871 mean_circ: 0.916131
Ran for 58050 frames Cell Events: 2898 mean_circ: 0.916129
Ran for 59050 frames Cell Events: 2943 mean_circ: 0.916051
Ran for 59550 frames Cell Events: 2968 mean_circ: 0.916037
Ran for 62050 frames Cell Events: 3092 mean_circ: 0.915821
Ran for 62550 frames Cell Events: 3128 mean_circ: 0.915797
Ran for 63550 frames Cell Events: 3191 mean_circ: 0.915706
Ran for 64050 frames Cell Events: 3224 mean_circ: 0.915808
Ran for 66050 frames Cell Events: 3324 mean_circ: 0.915827
Ran for 67550 frames Cell Events: 3419 mean_circ: 0.91578
Ran for 68550 frames Cell Events: 3437 mean_circ: 0.915746
Ran for 69050 frames Cell Events: 3463 mean_circ: 0.915896
Ran for 70050 frames Cell Events: 3507 mean_circ: 0.915937
Ran for 70550 frames Cell Events: 3523 mean_circ: 0.915834
Ran for 72050 frames Cell Events: 3625 mean_circ: 0.915984
Ran for 72550 frames Cell Events: 3660 mean_circ: 0.915962
Ran for 73050 frames Cell Events: 3691 mean_circ: 0.915844
Ran for 74050 frames Cell Events: 3751 mean_circ: 0.915963
Ran for 74550 frames Cell Events: 3773 mean_circ: 0.916046
Ran for 76050 frames Cell Events: 3873 mean_circ: 0.916171
Ran for 76550 frames Cell Events: 3888 mean_circ: 0.916208
Ran for 77050 frames Cell Events: 3906 mean_circ: 0.916156
Ran for 78050 frames Cell Events: 3957 mean_circ: 0.916124
Ran for 78550 frames Cell Events: 3993 mean_circ: 0.916047
Ran for 79050 frames Cell Events: 4024 mean_circ: 0.916076
Ran for 79550 frames Cell Events: 4044 mean_circ: 0.916086
Ran for 81550 frames Cell Events: 4154 mean_circ: 0.91596
Ran for 82550 frames Cell Events: 4224 mean_circ: 0.915741
Ran for 83550 frames Cell Events: 4267 mean_circ: 0.915707
Ran for 84050 frames Cell Events: 4289 mean_circ: 0.91582
Ran for 84550 frames Cell Events: 4319 mean_circ: 0.915771
Ran for 86050 frames Cell Events: 4396 mean_circ: 0.915737
Ran for 86550 frames Cell Events: 4423 mean_circ: 0.915778
Ran for 88050 frames Cell Events: 4524 mean_circ: 0.915684
Ran for 88550 frames Cell Events: 4542 mean_circ: 0.915661
Ran for 90550 frames Cell Events: 4675 mean_circ: 0.915662
Ran for 91050 frames Cell Events: 4697 mean_circ: 0.915699
Ran for 91550 frames Cell Events: 4727 mean_circ: 0.915699
Ran for 93050 frames Cell Events: 4832 mean_circ: 0.91594
Ran for 95550 frames Cell Events: 4978 mean_circ: 0.915866
Ran for 96050 frames Cell Events: 4987 mean_circ: 0.915882
Ran for 96550 frames Cell Events: 5001 mean_circ: 0.915874
Ran for 97050 frames Cell Events: 5034 mean_circ: 0.915922
Ran for 98050 frames Cell Events: 5102 mean_circ: 0.915829
Ran for 98550 frames Cell Events: 5129 mean_circ: 0.915863
Ran for 99050 frames Cell Events: 5158 mean_circ: 0.915896
Ran for 99550 frames Cell Events: 5183 mean_circ: 0.915877
Ran for 100050 frames Cell Events: 5212 mean_circ: 0.915787
Ran for 100550 frames Cell Events: 5244 mean_circ: 0.915761
Ran for 101050 frames Cell Events: 5269 mean_circ: 0.915784
Ran for 103550 frames Cell Events: 5414 mean_circ: 0.915745
Ran for 104050 frames Cell Events: 5439 mean_circ: 0.915743
Ran for 104550 frames Cell Events: 5465 mean_circ: 0.915758
Ran for 106050 frames Cell Events: 5558 mean_circ: 0.915806
Ran for 106550 frames Cell Events: 5580 mean_circ: 0.915834
Ran for 107050 frames Cell Events: 5604 mean_circ: 0.915866
Ran for 107550 frames Cell Events: 5631 mean_circ: 0.915821
Ran for 108550 frames Cell Events: 5699 mean_circ: 0.915768
Ran for 109050 frames Cell Events: 5732 mean_circ: 0.915774
Ran for 110050 frames Cell Events: 5776 mean_circ: 0.915819
Ran for 110550 frames Cell Events: 5807 mean_circ: 0.915819
Ran for 111550 frames Cell Events: 5843 mean_circ: 0.915814
Ran for 112550 frames Cell Events: 5889 mean_circ: 0.915794
Ran for 113050 frames Cell Events: 5916 mean_circ: 0.915742
Ran for 113550 frames Cell Events: 5941 mean_circ: 0.915707
Ran for 114550 frames Cell Events: 5994 mean_circ: 0.915713
Ran for 115050 frames Cell Events: 6018 mean_circ: 0.915769
Ran for 115550 frames Cell Events: 6034 mean_circ: 0.915781
Ran for 116050 frames Cell Events: 6058 mean_circ: 0.915758
Ran for 117050 frames Cell Events: 6103 mean_circ: 0.915814
Ran for 118550 frames Cell Events: 6185 mean_circ: 0.915764
Ran for 119550 frames Cell Events: 6245 mean_circ: 0.915723
Ran for 120050 frames Cell Events: 6268 mean_circ: 0.915644
Ran for 120550 frames Cell Events: 6297 mean_circ: 0.915658
Ran for 121050 frames Cell Events: 6319 mean_circ: 0.915698
Ran for 122550 frames Cell Events: 6395 mean_circ: 0.915843
Ran for 123050 frames Cell Events: 6423 mean_circ: 0.915819
Ran for 124050 frames Cell Events: 6469 mean_circ: 0.915765
Ran for 124550 frames Cell Events: 6504 mean_circ: 0.915765
Ran for 125050 frames Cell Events: 6532 mean_circ: 0.915802
Ran for 126050 frames Cell Events: 6589 mean_circ: 0.915859
Ran for 126550 frames Cell Events: 6621 mean_circ: 0.915827
Ran for 127550 frames Cell Events: 6673 mean_circ: 0.91584
Ran for 129050 frames Cell Events: 6762 mean_circ: 0.915909
Ran for 131550 frames Cell Events: 6905 mean_circ: 0.915956
Ran for 133050 frames Cell Events: 7014 mean_circ: 0.915913
Ran for 133550 frames Cell Events: 7043 mean_circ: 0.915884
Ran for 134550 frames Cell Events: 7089 mean_circ: 0.915849
Ran for 135050 frames Cell Events: 7108 mean_circ: 0.915838
Ran for 138050 frames Cell Events: 7293 mean_circ: 0.915884
Ran for 141050 frames Cell Events: 7418 mean_circ: 0.915959
Ran for 142050 frames Cell Events: 7476 mean_circ: 0.915993
Ran for 142550 frames Cell Events: 7511 mean_circ: 0.915968
Ran for 143050 frames Cell Events: 7547 mean_circ: 0.916017
Ran for 144550 frames Cell Events: 7618 mean_circ: 0.915942
Ran for 145050 frames Cell Events: 7650 mean_circ: 0.915869
Ran for 147050 frames Cell Events: 7778 mean_circ: 0.915915
Ran for 147550 frames Cell Events: 7809 mean_circ: 0.915894
Ran for 148550 frames Cell Events: 7887 mean_circ: 0.915868
Ran for 149050 frames Cell Events: 7918 mean_circ: 0.915795
Ran for 150550 frames Cell Events: 8004 mean_circ: 0.915879
Ran for 151550 frames Cell Events: 8062 mean_circ: 0.915914



End this measurment..



Stopping time: 1.6668e+009
****************************************
Measurement finished

Mean Circularitiy: 0.915909
Mean Area: 808.181
Mean FL-1 intensity: 2891.26
Mean FL-2 intensity: 3393.92
Mean FL-3 intensity: 0

frames processed: 148630
Total Time: 75847.2ms
Number of Runs (new bg img) 0
Frames collected: 151575
Skipped frames: 19733
Rescued Frames: 672123
Samples DAQ: 37894233
Samples written: 75787662
Samples per Channel: 37893831
FPS: 1998.43

Samples per second: 499608
Expected samples/s: 500000

Events: 8064
Events per second: 106.319
jumped back (PMT): 16
#Frames read, frequency
# 0 frames :230989
# 1 frames :59259
# 2 frames :3021
# 3 frames :1210
# 4 frames :594
# 5 frames :292
# 6 frames :1968
# 7 frames :1221
# 8 frames :1095
# 9 frames :615
# 10 frames :439
# 11 frames :358
# 12 frames :263
# 13 frames :204
# 14 frames :241
# 15 frames :184
# 16 frames :162
# 17 frames :129
# 18 frames :98
# 19 frames :87
# 20 frames :74
# 21 frames :58
# 22 frames :62
# 23 frames :50
# 24 frames :56
# 25 frames :32
# 26 frames :20
# 27 frames :17
# 28 frames :24
# 29 frames :21
# 30 frames :9
# 31 frames :13
# 32 frames :9
# 33 frames :6
# 34 frames :7
# 35 frames :6
# 36 frames :5
# 37 frames :5
# 38 frames :5
# 39 frames :1
# 40 frames :9
# 41 frames :3
# 42 frames :2
# 43 frames :2
# 44 frames :2
# 45 frames :2
# 46 frames :1
# 47 frames :1
# 51 frames :1
# 52 frames :3
# 53 frames :2
# 55 frames :3
# 56 frames :3
# 59 frames :1
# 61 frames :1
# 70 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.1534
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0