Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_12.05.58 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "8611cdb17bef05d48cd49a431edab372",
"name": "M1_20170603_1533_dsRNA_C4_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "10:06:22"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 10000 (max 10001) in
'10000'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.1991
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.66742e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 58 mean_circ: 0.94114
Ran for 2550 frames Cell Events: 154 mean_circ: 0.94011
Ran for 3050 frames Cell Events: 189 mean_circ: 0.938903
Ran for 7050 frames Cell Events: 399 mean_circ: 0.939502
Ran for 7550 frames Cell Events: 435 mean_circ: 0.939803
Ran for 8050 frames Cell Events: 462 mean_circ: 0.939871
Ran for 8550 frames Cell Events: 490 mean_circ: 0.940087
Ran for 9050 frames Cell Events: 518 mean_circ: 0.940593
Ran for 13050 frames Cell Events: 789 mean_circ: 0.940608
Ran for 14550 frames Cell Events: 881 mean_circ: 0.9402
Ran for 15050 frames Cell Events: 918 mean_circ: 0.94028
Ran for 16550 frames Cell Events: 1022 mean_circ: 0.940657
Ran for 17050 frames Cell Events: 1055 mean_circ: 0.940648
Ran for 18050 frames Cell Events: 1119 mean_circ: 0.940721
Ran for 19050 frames Cell Events: 1179 mean_circ: 0.940943
Ran for 19550 frames Cell Events: 1207 mean_circ: 0.940721
Ran for 20050 frames Cell Events: 1239 mean_circ: 0.940462
Ran for 20550 frames Cell Events: 1264 mean_circ: 0.940463
Ran for 21050 frames Cell Events: 1300 mean_circ: 0.940666
Ran for 21550 frames Cell Events: 1335 mean_circ: 0.940669
Ran for 22550 frames Cell Events: 1401 mean_circ: 0.940492
Ran for 23050 frames Cell Events: 1430 mean_circ: 0.940133
Ran for 25050 frames Cell Events: 1542 mean_circ: 0.939938
Ran for 25550 frames Cell Events: 1568 mean_circ: 0.939998
Ran for 27550 frames Cell Events: 1684 mean_circ: 0.940419
Ran for 28550 frames Cell Events: 1745 mean_circ: 0.940456
Ran for 29050 frames Cell Events: 1779 mean_circ: 0.940549
Ran for 30550 frames Cell Events: 1884 mean_circ: 0.940864
Ran for 31050 frames Cell Events: 1903 mean_circ: 0.940883
Ran for 32550 frames Cell Events: 1992 mean_circ: 0.940796
Ran for 33050 frames Cell Events: 2018 mean_circ: 0.940727
Ran for 33550 frames Cell Events: 2037 mean_circ: 0.940711
Ran for 34550 frames Cell Events: 2095 mean_circ: 0.940603
Ran for 37050 frames Cell Events: 2241 mean_circ: 0.940386
Ran for 37550 frames Cell Events: 2264 mean_circ: 0.9404
Ran for 39550 frames Cell Events: 2394 mean_circ: 0.940267
Ran for 40050 frames Cell Events: 2422 mean_circ: 0.940316
Ran for 40550 frames Cell Events: 2456 mean_circ: 0.940369
Ran for 41050 frames Cell Events: 2484 mean_circ: 0.940436
Ran for 41550 frames Cell Events: 2513 mean_circ: 0.940485
Ran for 42550 frames Cell Events: 2534 mean_circ: 0.940331
Ran for 43050 frames Cell Events: 2573 mean_circ: 0.940189
Ran for 43550 frames Cell Events: 2607 mean_circ: 0.940208
Ran for 44050 frames Cell Events: 2634 mean_circ: 0.940243
Ran for 44550 frames Cell Events: 2672 mean_circ: 0.940147
Ran for 46050 frames Cell Events: 2748 mean_circ: 0.94027
Ran for 47050 frames Cell Events: 2805 mean_circ: 0.940183
Ran for 47550 frames Cell Events: 2832 mean_circ: 0.939974
Ran for 48050 frames Cell Events: 2866 mean_circ: 0.939932
Ran for 48550 frames Cell Events: 2907 mean_circ: 0.939964
Ran for 50550 frames Cell Events: 3031 mean_circ: 0.940172
Ran for 52050 frames Cell Events: 3118 mean_circ: 0.940183
Ran for 52550 frames Cell Events: 3145 mean_circ: 0.940117
Ran for 53050 frames Cell Events: 3168 mean_circ: 0.940172
Ran for 53550 frames Cell Events: 3195 mean_circ: 0.940212
Ran for 55050 frames Cell Events: 3256 mean_circ: 0.940079
Ran for 56550 frames Cell Events: 3338 mean_circ: 0.939984
Ran for 57050 frames Cell Events: 3364 mean_circ: 0.93984
Ran for 57550 frames Cell Events: 3399 mean_circ: 0.939809
Ran for 58050 frames Cell Events: 3433 mean_circ: 0.939826
Ran for 58550 frames Cell Events: 3470 mean_circ: 0.939856
Ran for 59050 frames Cell Events: 3488 mean_circ: 0.939864
Ran for 59550 frames Cell Events: 3527 mean_circ: 0.939896
Ran for 62050 frames Cell Events: 3643 mean_circ: 0.93989
Ran for 62550 frames Cell Events: 3672 mean_circ: 0.939923
Ran for 63550 frames Cell Events: 3739 mean_circ: 0.940052
Ran for 65550 frames Cell Events: 3847 mean_circ: 0.939987
Ran for 66550 frames Cell Events: 3911 mean_circ: 0.940031
Ran for 67050 frames Cell Events: 3948 mean_circ: 0.940061
Ran for 68550 frames Cell Events: 4001 mean_circ: 0.939984
Ran for 69050 frames Cell Events: 4027 mean_circ: 0.940027
Ran for 69550 frames Cell Events: 4060 mean_circ: 0.94004
Ran for 70550 frames Cell Events: 4124 mean_circ: 0.940075
Ran for 72050 frames Cell Events: 4216 mean_circ: 0.940208
Ran for 72550 frames Cell Events: 4233 mean_circ: 0.940181
Ran for 73050 frames Cell Events: 4278 mean_circ: 0.940142
Ran for 74050 frames Cell Events: 4327 mean_circ: 0.940132
Ran for 74550 frames Cell Events: 4357 mean_circ: 0.940207
Ran for 75550 frames Cell Events: 4411 mean_circ: 0.940272
Ran for 76550 frames Cell Events: 4476 mean_circ: 0.940121
Ran for 77550 frames Cell Events: 4540 mean_circ: 0.940157
Ran for 78050 frames Cell Events: 4572 mean_circ: 0.940173
Ran for 79050 frames Cell Events: 4637 mean_circ: 0.940209
Ran for 79550 frames Cell Events: 4671 mean_circ: 0.940186
Ran for 82050 frames Cell Events: 4825 mean_circ: 0.940129
Ran for 82550 frames Cell Events: 4868 mean_circ: 0.940035
Ran for 83050 frames Cell Events: 4901 mean_circ: 0.940078
Ran for 85050 frames Cell Events: 5048 mean_circ: 0.940039
Ran for 87050 frames Cell Events: 5184 mean_circ: 0.939869
Ran for 88550 frames Cell Events: 5284 mean_circ: 0.939886
Ran for 89550 frames Cell Events: 5361 mean_circ: 0.939939
Ran for 90050 frames Cell Events: 5404 mean_circ: 0.939903
Ran for 92050 frames Cell Events: 5521 mean_circ: 0.940017
Ran for 92550 frames Cell Events: 5559 mean_circ: 0.940018
Ran for 94550 frames Cell Events: 5678 mean_circ: 0.940116
Ran for 95550 frames Cell Events: 5741 mean_circ: 0.94015
Ran for 96050 frames Cell Events: 5768 mean_circ: 0.940132
Ran for 96550 frames Cell Events: 5796 mean_circ: 0.940126
Ran for 98550 frames Cell Events: 5921 mean_circ: 0.940234
Ran for 99050 frames Cell Events: 5949 mean_circ: 0.940264
Ran for 100550 frames Cell Events: 6050 mean_circ: 0.940236
Ran for 101050 frames Cell Events: 6070 mean_circ: 0.940202
Ran for 101550 frames Cell Events: 6099 mean_circ: 0.940189
Ran for 103050 frames Cell Events: 6192 mean_circ: 0.940205
Ran for 103550 frames Cell Events: 6226 mean_circ: 0.940181
Ran for 104050 frames Cell Events: 6252 mean_circ: 0.940242
Ran for 105050 frames Cell Events: 6308 mean_circ: 0.940263
Ran for 106050 frames Cell Events: 6370 mean_circ: 0.940246
Ran for 106550 frames Cell Events: 6406 mean_circ: 0.940218
Ran for 107050 frames Cell Events: 6438 mean_circ: 0.940206
Ran for 109550 frames Cell Events: 6604 mean_circ: 0.940245
Ran for 110050 frames Cell Events: 6640 mean_circ: 0.940193
Ran for 110550 frames Cell Events: 6674 mean_circ: 0.940121
Ran for 111550 frames Cell Events: 6726 mean_circ: 0.940111
Ran for 112050 frames Cell Events: 6766 mean_circ: 0.940108
Ran for 113550 frames Cell Events: 6858 mean_circ: 0.940121
Ran for 114050 frames Cell Events: 6890 mean_circ: 0.940132
Ran for 115550 frames Cell Events: 6973 mean_circ: 0.940137
Ran for 116050 frames Cell Events: 7005 mean_circ: 0.940051
Ran for 116550 frames Cell Events: 7035 mean_circ: 0.940079
Ran for 117050 frames Cell Events: 7075 mean_circ: 0.940124
Ran for 117550 frames Cell Events: 7102 mean_circ: 0.940174
Ran for 120050 frames Cell Events: 7227 mean_circ: 0.940038
Ran for 120550 frames Cell Events: 7250 mean_circ: 0.940076
Ran for 121050 frames Cell Events: 7280 mean_circ: 0.940066
Ran for 122050 frames Cell Events: 7338 mean_circ: 0.940051
Ran for 123050 frames Cell Events: 7406 mean_circ: 0.939942
Ran for 123550 frames Cell Events: 7428 mean_circ: 0.939943
Ran for 125050 frames Cell Events: 7548 mean_circ: 0.939912
Ran for 125550 frames Cell Events: 7574 mean_circ: 0.939934
Ran for 126550 frames Cell Events: 7650 mean_circ: 0.939935
Ran for 127050 frames Cell Events: 7679 mean_circ: 0.93985
Ran for 127550 frames Cell Events: 7710 mean_circ: 0.939845
Ran for 128050 frames Cell Events: 7744 mean_circ: 0.939817
Ran for 129050 frames Cell Events: 7794 mean_circ: 0.939841
Ran for 130550 frames Cell Events: 7886 mean_circ: 0.939868
Ran for 131050 frames Cell Events: 7918 mean_circ: 0.939794
Ran for 132050 frames Cell Events: 7979 mean_circ: 0.939809
Ran for 133050 frames Cell Events: 8054 mean_circ: 0.939838
Ran for 134550 frames Cell Events: 8144 mean_circ: 0.939813
Ran for 135050 frames Cell Events: 8174 mean_circ: 0.939829
Ran for 135550 frames Cell Events: 8210 mean_circ: 0.939841
Ran for 137050 frames Cell Events: 8285 mean_circ: 0.939758
Ran for 137550 frames Cell Events: 8315 mean_circ: 0.939775
Ran for 139550 frames Cell Events: 8459 mean_circ: 0.939803
Ran for 140050 frames Cell Events: 8491 mean_circ: 0.939831
Ran for 140550 frames Cell Events: 8518 mean_circ: 0.939783
Ran for 142550 frames Cell Events: 8643 mean_circ: 0.939809
Ran for 143050 frames Cell Events: 8663 mean_circ: 0.939754
Ran for 144550 frames Cell Events: 8786 mean_circ: 0.939778
Ran for 146050 frames Cell Events: 8882 mean_circ: 0.939828
Ran for 146550 frames Cell Events: 8918 mean_circ: 0.939846
Ran for 150550 frames Cell Events: 9190 mean_circ: 0.939945
Ran for 152050 frames Cell Events: 9296 mean_circ: 0.93998
Ran for 153050 frames Cell Events: 9359 mean_circ: 0.939914
Ran for 153550 frames Cell Events: 9384 mean_circ: 0.939914
Ran for 154550 frames Cell Events: 9458 mean_circ: 0.93987
Ran for 155050 frames Cell Events: 9496 mean_circ: 0.939883
Ran for 155550 frames Cell Events: 9527 mean_circ: 0.939895
Ran for 156050 frames Cell Events: 9551 mean_circ: 0.939909
Ran for 156550 frames Cell Events: 9563 mean_circ: 0.93986
Ran for 157050 frames Cell Events: 9599 mean_circ: 0.939883
Ran for 157550 frames Cell Events: 9629 mean_circ: 0.939897
Ran for 158550 frames Cell Events: 9712 mean_circ: 0.93991
Ran for 159050 frames Cell Events: 9741 mean_circ: 0.939924
Ran for 159550 frames Cell Events: 9775 mean_circ: 0.93994
Ran for 160550 frames Cell Events: 9871 mean_circ: 0.939948
Ran for 161050 frames Cell Events: 9902 mean_circ: 0.939958
Ran for 161550 frames Cell Events: 9929 mean_circ: 0.93997
Ran for 162550 frames Cell Events: 9982 mean_circ: 0.939987



End this measurment.
because stop condition (time/nr of cells) was met

Stopping time: 1.6675e+009
****************************************
Measurement finished

Mean Circularitiy: 0.939995
Mean Area: 602.044
Mean FL-1 intensity: 3407.07
Mean FL-2 intensity: 3466.13
Mean FL-3 intensity: 0

frames processed: 159675
Total Time: 81524.6ms
Number of Runs (new bg img) 0
Frames collected: 162900
Skipped frames: 24617
Rescued Frames: 861119
Samples DAQ: 40727104
Samples written: 81449854
Samples per Channel: 40724927
FPS: 1998.17

Samples per second: 499541
Expected samples/s: 500000

Events: 10001
Events per second: 122.675
jumped back (PMT): 31
#Frames read, frequency
# 0 frames :255801
# 1 frames :55563
# 2 frames :3092
# 3 frames :1289
# 4 frames :609
# 5 frames :313
# 6 frames :2625
# 7 frames :1321
# 8 frames :1302
# 9 frames :757
# 10 frames :470
# 11 frames :348
# 12 frames :301
# 13 frames :221
# 14 frames :314
# 15 frames :209
# 16 frames :173
# 17 frames :154
# 18 frames :128
# 19 frames :93
# 20 frames :93
# 21 frames :88
# 22 frames :69
# 23 frames :54
# 24 frames :54
# 25 frames :24
# 26 frames :33
# 27 frames :29
# 28 frames :22
# 29 frames :21
# 30 frames :16
# 31 frames :13
# 32 frames :10
# 33 frames :19
# 34 frames :13
# 35 frames :10
# 36 frames :9
# 37 frames :4
# 38 frames :8
# 39 frames :8
# 40 frames :7
# 41 frames :3
# 42 frames :2
# 43 frames :1
# 44 frames :3
# 45 frames :3
# 47 frames :1
# 49 frames :1
# 51 frames :1
# 52 frames :1
# 54 frames :2
# 56 frames :1
# 61 frames :2
# 91 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.1991
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0