Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_12.08.00 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "bd3cbce28fd95daf3bcb12daf1d8d98c",
"name": "M1_20170603_1557_NK1_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "10:08:24"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 9851 (max 9852) in '9851'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.2853
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.66889e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 28 mean_circ: 0.949246
Ran for 2050 frames Cell Events: 117 mean_circ: 0.94007
Ran for 3550 frames Cell Events: 233 mean_circ: 0.934002
Ran for 4550 frames Cell Events: 317 mean_circ: 0.93315
Ran for 6550 frames Cell Events: 456 mean_circ: 0.933012
Ran for 7050 frames Cell Events: 475 mean_circ: 0.933341
Ran for 8050 frames Cell Events: 533 mean_circ: 0.932156
Ran for 10550 frames Cell Events: 685 mean_circ: 0.932754
Ran for 11550 frames Cell Events: 766 mean_circ: 0.933362
Ran for 12050 frames Cell Events: 796 mean_circ: 0.933493
Ran for 13550 frames Cell Events: 903 mean_circ: 0.933076
Ran for 15050 frames Cell Events: 997 mean_circ: 0.933887
Ran for 16550 frames Cell Events: 1091 mean_circ: 0.933663
Ran for 18550 frames Cell Events: 1244 mean_circ: 0.933817
Ran for 19050 frames Cell Events: 1283 mean_circ: 0.933957
Ran for 19550 frames Cell Events: 1318 mean_circ: 0.934027
Ran for 20050 frames Cell Events: 1349 mean_circ: 0.933829
Ran for 23550 frames Cell Events: 1599 mean_circ: 0.933875
Ran for 25050 frames Cell Events: 1711 mean_circ: 0.933773
Ran for 28050 frames Cell Events: 1935 mean_circ: 0.934584
Ran for 28550 frames Cell Events: 1970 mean_circ: 0.934362
Ran for 31550 frames Cell Events: 2194 mean_circ: 0.934383
Ran for 32050 frames Cell Events: 2241 mean_circ: 0.934501
Ran for 33550 frames Cell Events: 2355 mean_circ: 0.934529
Ran for 35050 frames Cell Events: 2480 mean_circ: 0.9342
Ran for 39550 frames Cell Events: 2850 mean_circ: 0.934702
Ran for 45050 frames Cell Events: 3270 mean_circ: 0.934687
Ran for 45550 frames Cell Events: 3303 mean_circ: 0.93475
Ran for 46550 frames Cell Events: 3385 mean_circ: 0.934796
Ran for 47050 frames Cell Events: 3407 mean_circ: 0.934955
Ran for 48050 frames Cell Events: 3472 mean_circ: 0.934999
Ran for 53050 frames Cell Events: 3877 mean_circ: 0.934594
Ran for 54050 frames Cell Events: 3949 mean_circ: 0.93465
Ran for 56050 frames Cell Events: 4108 mean_circ: 0.93459
Ran for 57050 frames Cell Events: 4178 mean_circ: 0.934682
Ran for 60050 frames Cell Events: 4375 mean_circ: 0.934808
Ran for 64050 frames Cell Events: 4682 mean_circ: 0.934757
Ran for 65050 frames Cell Events: 4779 mean_circ: 0.934612
Ran for 65550 frames Cell Events: 4818 mean_circ: 0.9347
Ran for 67050 frames Cell Events: 4930 mean_circ: 0.934653
Ran for 68050 frames Cell Events: 5016 mean_circ: 0.934692
Ran for 69050 frames Cell Events: 5090 mean_circ: 0.934566
Ran for 71050 frames Cell Events: 5227 mean_circ: 0.934762
Ran for 73050 frames Cell Events: 5371 mean_circ: 0.934572
Ran for 75050 frames Cell Events: 5539 mean_circ: 0.934376
Ran for 76550 frames Cell Events: 5657 mean_circ: 0.93439
Ran for 77050 frames Cell Events: 5691 mean_circ: 0.934396
Ran for 77550 frames Cell Events: 5717 mean_circ: 0.9344
Ran for 78050 frames Cell Events: 5770 mean_circ: 0.9344
Ran for 78550 frames Cell Events: 5805 mean_circ: 0.934255
Ran for 79550 frames Cell Events: 5875 mean_circ: 0.934146
Ran for 80550 frames Cell Events: 5963 mean_circ: 0.934111
Ran for 83050 frames Cell Events: 6158 mean_circ: 0.933992
Ran for 86050 frames Cell Events: 6396 mean_circ: 0.933941
Ran for 87550 frames Cell Events: 6498 mean_circ: 0.933918
Ran for 89050 frames Cell Events: 6596 mean_circ: 0.933999
Ran for 89550 frames Cell Events: 6638 mean_circ: 0.934019
Ran for 90550 frames Cell Events: 6717 mean_circ: 0.933927
Ran for 91050 frames Cell Events: 6758 mean_circ: 0.933927
Ran for 93050 frames Cell Events: 6908 mean_circ: 0.933834
Ran for 94050 frames Cell Events: 6989 mean_circ: 0.933898
Ran for 95050 frames Cell Events: 7052 mean_circ: 0.933809
Ran for 95550 frames Cell Events: 7083 mean_circ: 0.933825
Ran for 97550 frames Cell Events: 7219 mean_circ: 0.93382
Ran for 98050 frames Cell Events: 7236 mean_circ: 0.933833
Ran for 99050 frames Cell Events: 7326 mean_circ: 0.933819
Ran for 99550 frames Cell Events: 7358 mean_circ: 0.933777
Ran for 102050 frames Cell Events: 7584 mean_circ: 0.933668
Ran for 104050 frames Cell Events: 7730 mean_circ: 0.933628
Ran for 104550 frames Cell Events: 7761 mean_circ: 0.933645
Ran for 105050 frames Cell Events: 7784 mean_circ: 0.933678
Ran for 106050 frames Cell Events: 7842 mean_circ: 0.933763
Ran for 108050 frames Cell Events: 7956 mean_circ: 0.933811
Ran for 109550 frames Cell Events: 8070 mean_circ: 0.933814
Ran for 110550 frames Cell Events: 8119 mean_circ: 0.933793
Ran for 111050 frames Cell Events: 8154 mean_circ: 0.933793
Ran for 113550 frames Cell Events: 8330 mean_circ: 0.933726
Ran for 114550 frames Cell Events: 8379 mean_circ: 0.933681
Ran for 119050 frames Cell Events: 8717 mean_circ: 0.933717
Ran for 119550 frames Cell Events: 8752 mean_circ: 0.93373
Ran for 120050 frames Cell Events: 8797 mean_circ: 0.933713
Ran for 120550 frames Cell Events: 8850 mean_circ: 0.933713
Ran for 121550 frames Cell Events: 8908 mean_circ: 0.93368
Ran for 123050 frames Cell Events: 9006 mean_circ: 0.933688
Ran for 123550 frames Cell Events: 9045 mean_circ: 0.933684
Ran for 125050 frames Cell Events: 9170 mean_circ: 0.933593
Ran for 126550 frames Cell Events: 9281 mean_circ: 0.933541
Ran for 128050 frames Cell Events: 9400 mean_circ: 0.933549
Ran for 128550 frames Cell Events: 9439 mean_circ: 0.933564
Ran for 133050 frames Cell Events: 9801 mean_circ: 0.933676



End this measurment..



Stopping time: 1.66895e+009
****************************************
Measurement finished

Mean Circularitiy: 0.933686
Mean Area: 635.081
Mean FL-1 intensity: 3222.05
Mean FL-2 intensity: 3376.95
Mean FL-3 intensity: 0

frames processed: 129767
Total Time: 66891.4ms
Number of Runs (new bg img) 0
Frames collected: 133597
Skipped frames: 37066
Rescued Frames: 1272507
Samples DAQ: 33402031
Samples written: 66798654
Samples per Channel: 33399327
FPS: 1997.22

Samples per second: 499307
Expected samples/s: 500000

Events: 9852
Events per second: 147.283
jumped back (PMT): 36
#Frames read, frequency
# 0 frames :211675
# 1 frames :28253
# 2 frames :1892
# 3 frames :788
# 4 frames :477
# 5 frames :253
# 6 frames :1263
# 7 frames :793
# 8 frames :749
# 9 frames :508
# 10 frames :548
# 11 frames :400
# 12 frames :276
# 13 frames :234
# 14 frames :219
# 15 frames :247
# 16 frames :238
# 17 frames :179
# 18 frames :164
# 19 frames :113
# 20 frames :106
# 21 frames :128
# 22 frames :103
# 23 frames :98
# 24 frames :69
# 25 frames :62
# 26 frames :65
# 27 frames :62
# 28 frames :53
# 29 frames :47
# 30 frames :39
# 31 frames :34
# 32 frames :34
# 33 frames :40
# 34 frames :27
# 35 frames :29
# 36 frames :22
# 37 frames :12
# 38 frames :21
# 39 frames :18
# 40 frames :24
# 41 frames :11
# 42 frames :7
# 43 frames :17
# 44 frames :16
# 45 frames :10
# 46 frames :14
# 47 frames :4
# 48 frames :9
# 49 frames :5
# 50 frames :8
# 51 frames :6
# 52 frames :3
# 53 frames :4
# 54 frames :5
# 55 frames :7
# 56 frames :3
# 57 frames :2
# 58 frames :2
# 59 frames :4
# 60 frames :2
# 61 frames :3
# 62 frames :3
# 64 frames :2
# 66 frames :2
# 68 frames :4
# 69 frames :1
# 71 frames :2
# 73 frames :1
# 75 frames :1
# 84 frames :1
# 98 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.2853
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0