Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_12.08.36 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "179b84a03ca8b8892921c66cfa7b0e14",
"name": "M1_20170603_1606_dsRNA_M2_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "10:08:56"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8150 (max 8151) in '8150'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.3034
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.6694e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 29 mean_circ: 0.955519
Ran for 1550 frames Cell Events: 79 mean_circ: 0.944798
Ran for 2050 frames Cell Events: 102 mean_circ: 0.94711
Ran for 2550 frames Cell Events: 116 mean_circ: 0.948945
Ran for 3550 frames Cell Events: 169 mean_circ: 0.949671
Ran for 7050 frames Cell Events: 367 mean_circ: 0.950125
Ran for 9050 frames Cell Events: 509 mean_circ: 0.947853
Ran for 10050 frames Cell Events: 567 mean_circ: 0.947345
Ran for 11050 frames Cell Events: 627 mean_circ: 0.948004
Ran for 12050 frames Cell Events: 714 mean_circ: 0.948135
Ran for 13050 frames Cell Events: 792 mean_circ: 0.948189
Ran for 13550 frames Cell Events: 819 mean_circ: 0.948512
Ran for 14050 frames Cell Events: 855 mean_circ: 0.948652
Ran for 14550 frames Cell Events: 875 mean_circ: 0.948836
Ran for 15550 frames Cell Events: 916 mean_circ: 0.948617
Ran for 16050 frames Cell Events: 945 mean_circ: 0.948741
Ran for 16550 frames Cell Events: 966 mean_circ: 0.948703
Ran for 17550 frames Cell Events: 1022 mean_circ: 0.948292
Ran for 19550 frames Cell Events: 1141 mean_circ: 0.948004
Ran for 21050 frames Cell Events: 1218 mean_circ: 0.947975
Ran for 22050 frames Cell Events: 1276 mean_circ: 0.948113
Ran for 22550 frames Cell Events: 1302 mean_circ: 0.948002
Ran for 24050 frames Cell Events: 1424 mean_circ: 0.947665
Ran for 25550 frames Cell Events: 1525 mean_circ: 0.947498
Ran for 28050 frames Cell Events: 1652 mean_circ: 0.946456
Ran for 29550 frames Cell Events: 1717 mean_circ: 0.946741
Ran for 31050 frames Cell Events: 1801 mean_circ: 0.946883
Ran for 31550 frames Cell Events: 1832 mean_circ: 0.947055
Ran for 32050 frames Cell Events: 1858 mean_circ: 0.946933
Ran for 32550 frames Cell Events: 1886 mean_circ: 0.946875
Ran for 33050 frames Cell Events: 1905 mean_circ: 0.946816
Ran for 34550 frames Cell Events: 1992 mean_circ: 0.946792
Ran for 35050 frames Cell Events: 2025 mean_circ: 0.946824
Ran for 36550 frames Cell Events: 2131 mean_circ: 0.946799
Ran for 39550 frames Cell Events: 2344 mean_circ: 0.946832
Ran for 42050 frames Cell Events: 2505 mean_circ: 0.946904
Ran for 42550 frames Cell Events: 2527 mean_circ: 0.946918
Ran for 43050 frames Cell Events: 2547 mean_circ: 0.946915
Ran for 43550 frames Cell Events: 2575 mean_circ: 0.946872
Ran for 44050 frames Cell Events: 2610 mean_circ: 0.946808
Ran for 45550 frames Cell Events: 2692 mean_circ: 0.946685
Ran for 48050 frames Cell Events: 2825 mean_circ: 0.946688
Ran for 49050 frames Cell Events: 2890 mean_circ: 0.946647
Ran for 49550 frames Cell Events: 2919 mean_circ: 0.946661
Ran for 50550 frames Cell Events: 2974 mean_circ: 0.946602
Ran for 52550 frames Cell Events: 3107 mean_circ: 0.946775
Ran for 53550 frames Cell Events: 3173 mean_circ: 0.946767
Ran for 54050 frames Cell Events: 3201 mean_circ: 0.946708
Ran for 54550 frames Cell Events: 3228 mean_circ: 0.946738
Ran for 57050 frames Cell Events: 3369 mean_circ: 0.946709
Ran for 58050 frames Cell Events: 3412 mean_circ: 0.946762
Ran for 59050 frames Cell Events: 3476 mean_circ: 0.946739
Ran for 59550 frames Cell Events: 3507 mean_circ: 0.946686
Ran for 60550 frames Cell Events: 3595 mean_circ: 0.946686
Ran for 61050 frames Cell Events: 3617 mean_circ: 0.946642
Ran for 62050 frames Cell Events: 3685 mean_circ: 0.946494
Ran for 64050 frames Cell Events: 3815 mean_circ: 0.946521
Ran for 64550 frames Cell Events: 3845 mean_circ: 0.946441
Ran for 66050 frames Cell Events: 3960 mean_circ: 0.946278
Ran for 67050 frames Cell Events: 4001 mean_circ: 0.946283
Ran for 67550 frames Cell Events: 4032 mean_circ: 0.946322
Ran for 68050 frames Cell Events: 4061 mean_circ: 0.946266
Ran for 69550 frames Cell Events: 4144 mean_circ: 0.946205
Ran for 70050 frames Cell Events: 4178 mean_circ: 0.946205
Ran for 73550 frames Cell Events: 4453 mean_circ: 0.945938
Ran for 74050 frames Cell Events: 4493 mean_circ: 0.945945
Ran for 75550 frames Cell Events: 4605 mean_circ: 0.945938
Ran for 76550 frames Cell Events: 4696 mean_circ: 0.946033
Ran for 77050 frames Cell Events: 4733 mean_circ: 0.945982
Ran for 78550 frames Cell Events: 4852 mean_circ: 0.945845
Ran for 80050 frames Cell Events: 4957 mean_circ: 0.945831
Ran for 81050 frames Cell Events: 5016 mean_circ: 0.94584
Ran for 83550 frames Cell Events: 5176 mean_circ: 0.945901
Ran for 84550 frames Cell Events: 5235 mean_circ: 0.945901
Ran for 85050 frames Cell Events: 5258 mean_circ: 0.945873
Ran for 86050 frames Cell Events: 5325 mean_circ: 0.945871
Ran for 87050 frames Cell Events: 5378 mean_circ: 0.945882
Ran for 89050 frames Cell Events: 5533 mean_circ: 0.945867
Ran for 90550 frames Cell Events: 5628 mean_circ: 0.945824
Ran for 92050 frames Cell Events: 5761 mean_circ: 0.945718
Ran for 94050 frames Cell Events: 5915 mean_circ: 0.945655
Ran for 95550 frames Cell Events: 6039 mean_circ: 0.945774
Ran for 96050 frames Cell Events: 6077 mean_circ: 0.945777
Ran for 96550 frames Cell Events: 6123 mean_circ: 0.945743
Ran for 97050 frames Cell Events: 6168 mean_circ: 0.945782
Ran for 98550 frames Cell Events: 6272 mean_circ: 0.9458
Ran for 99550 frames Cell Events: 6351 mean_circ: 0.9458
Ran for 101050 frames Cell Events: 6446 mean_circ: 0.945762
Ran for 101550 frames Cell Events: 6483 mean_circ: 0.945821
Ran for 102050 frames Cell Events: 6523 mean_circ: 0.945818
Ran for 103550 frames Cell Events: 6632 mean_circ: 0.945748
Ran for 105550 frames Cell Events: 6801 mean_circ: 0.945737
Ran for 107550 frames Cell Events: 6962 mean_circ: 0.945777
Ran for 109050 frames Cell Events: 7079 mean_circ: 0.945787
Ran for 110050 frames Cell Events: 7159 mean_circ: 0.945739
Ran for 114550 frames Cell Events: 7508 mean_circ: 0.945774
Ran for 116050 frames Cell Events: 7621 mean_circ: 0.945715
Ran for 116550 frames Cell Events: 7658 mean_circ: 0.945715
Ran for 118050 frames Cell Events: 7770 mean_circ: 0.945625
Ran for 121050 frames Cell Events: 8013 mean_circ: 0.945517
Ran for 122550 frames Cell Events: 8127 mean_circ: 0.945488



End this measurment..



Stopping time: 1.66946e+009
****************************************
Measurement finished

Mean Circularitiy: 0.945503
Mean Area: 647.575
Mean FL-1 intensity: 5075.99
Mean FL-2 intensity: 3675.64
Mean FL-3 intensity: 0

frames processed: 119772
Total Time: 61536.8ms
Number of Runs (new bg img) 0
Frames collected: 122858
Skipped frames: 40152
Rescued Frames: 1351007
Samples DAQ: 30716948
Samples written: 61428986
Samples per Channel: 30714493
FPS: 1996.5

Samples per second: 499124
Expected samples/s: 500000

Events: 8151
Events per second: 132.457
jumped back (PMT): 2476
#Frames read, frequency
# 0 frames :195190
# 1 frames :31510
# 2 frames :2018
# 3 frames :799
# 4 frames :551
# 5 frames :229
# 6 frames :934
# 7 frames :852
# 8 frames :661
# 9 frames :404
# 10 frames :484
# 11 frames :379
# 12 frames :261
# 13 frames :211
# 14 frames :204
# 15 frames :234
# 16 frames :207
# 17 frames :157
# 18 frames :153
# 19 frames :91
# 20 frames :79
# 21 frames :85
# 22 frames :84
# 23 frames :83
# 24 frames :68
# 25 frames :54
# 26 frames :43
# 27 frames :39
# 28 frames :39
# 29 frames :31
# 30 frames :34
# 31 frames :34
# 32 frames :22
# 33 frames :26
# 34 frames :28
# 35 frames :25
# 36 frames :15
# 37 frames :21
# 38 frames :15
# 39 frames :13
# 40 frames :16
# 41 frames :14
# 42 frames :10
# 43 frames :13
# 44 frames :8
# 45 frames :8
# 46 frames :5
# 47 frames :7
# 48 frames :5
# 49 frames :3
# 50 frames :6
# 51 frames :6
# 52 frames :4
# 53 frames :6
# 54 frames :2
# 55 frames :1
# 56 frames :4
# 57 frames :2
# 58 frames :3
# 59 frames :3
# 60 frames :4
# 61 frames :2
# 62 frames :2
# 64 frames :2
# 66 frames :1
# 67 frames :1
# 69 frames :1
# 71 frames :1
# 73 frames :1
# 80 frames :2
# 81 frames :1
# 96 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.3034
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0