Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_12.09.03 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "0b95794724c4e22fe56a0149f40fccc7",
"name": "M1_20170603_1608_dsRNA_M2_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "10:09:27"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 10003 (max 10005) in
'10003'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.3046
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.66954e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 5050 frames Cell Events: 433 mean_circ: 0.93042
Ran for 5550 frames Cell Events: 475 mean_circ: 0.93042
Ran for 6050 frames Cell Events: 518 mean_circ: 0.93042
Ran for 10550 frames Cell Events: 902 mean_circ: 0.931898
Ran for 11550 frames Cell Events: 978 mean_circ: 0.931591
Ran for 16550 frames Cell Events: 1413 mean_circ: 0.931666
Ran for 18550 frames Cell Events: 1576 mean_circ: 0.931671
Ran for 21550 frames Cell Events: 1837 mean_circ: 0.932029
Ran for 22550 frames Cell Events: 1936 mean_circ: 0.932127
Ran for 25050 frames Cell Events: 2171 mean_circ: 0.931958
Ran for 29550 frames Cell Events: 2600 mean_circ: 0.932001
Ran for 37050 frames Cell Events: 3247 mean_circ: 0.931987
Ran for 38550 frames Cell Events: 3387 mean_circ: 0.932053
Ran for 39550 frames Cell Events: 3471 mean_circ: 0.932287
Ran for 42050 frames Cell Events: 3696 mean_circ: 0.932381
Ran for 43050 frames Cell Events: 3782 mean_circ: 0.93236
Ran for 43550 frames Cell Events: 3812 mean_circ: 0.932308
Ran for 45050 frames Cell Events: 3929 mean_circ: 0.932375
Ran for 47550 frames Cell Events: 4152 mean_circ: 0.932361
Ran for 48050 frames Cell Events: 4193 mean_circ: 0.932337
Ran for 52550 frames Cell Events: 4570 mean_circ: 0.932217
Ran for 53550 frames Cell Events: 4652 mean_circ: 0.93215
Ran for 54550 frames Cell Events: 4750 mean_circ: 0.932074
Ran for 55550 frames Cell Events: 4827 mean_circ: 0.932191
Ran for 56550 frames Cell Events: 4925 mean_circ: 0.932177
Ran for 58550 frames Cell Events: 5119 mean_circ: 0.93211
Ran for 62050 frames Cell Events: 5466 mean_circ: 0.931983
Ran for 65050 frames Cell Events: 5782 mean_circ: 0.931844
Ran for 67550 frames Cell Events: 5969 mean_circ: 0.931728
Ran for 68550 frames Cell Events: 6058 mean_circ: 0.931682
Ran for 70550 frames Cell Events: 6243 mean_circ: 0.931814
Ran for 73550 frames Cell Events: 6502 mean_circ: 0.93186
Ran for 76550 frames Cell Events: 6772 mean_circ: 0.931872
Ran for 77050 frames Cell Events: 6811 mean_circ: 0.931891
Ran for 83050 frames Cell Events: 7379 mean_circ: 0.932017
Ran for 84050 frames Cell Events: 7467 mean_circ: 0.931982
Ran for 85550 frames Cell Events: 7614 mean_circ: 0.931948
Ran for 87050 frames Cell Events: 7752 mean_circ: 0.931882
Ran for 90050 frames Cell Events: 8044 mean_circ: 0.931876
Ran for 91050 frames Cell Events: 8130 mean_circ: 0.931869
Ran for 91550 frames Cell Events: 8183 mean_circ: 0.931852
Ran for 96550 frames Cell Events: 8627 mean_circ: 0.9319
Ran for 100050 frames Cell Events: 8991 mean_circ: 0.932046
Ran for 103050 frames Cell Events: 9301 mean_circ: 0.932012
Ran for 105050 frames Cell Events: 9495 mean_circ: 0.932013
Ran for 107550 frames Cell Events: 9728 mean_circ: 0.931894
Ran for 109550 frames Cell Events: 9912 mean_circ: 0.931873



End this measurment.
because stop condition (time/nr of cells) was met

Stopping time: 1.6696e+009
****************************************
Measurement finished

Mean Circularitiy: 0.93189
Mean Area: 642.232
Mean FL-1 intensity: 4861.78
Mean FL-2 intensity: 3990.96
Mean FL-3 intensity: 0

frames processed: 103656
Total Time: 55354.7ms
Number of Runs (new bg img) 0
Frames collected: 110491
Skipped frames: 46987
Rescued Frames: 1430323
Samples DAQ: 27633065
Samples written: 55245238
Samples per Channel: 27622619
FPS: 1996.05

Samples per second: 499011
Expected samples/s: 500000

Events: 10005
Events per second: 180.743
jumped back (PMT): 41
#Frames read, frequency
# 0 frames :124459
# 1 frames :16315
# 2 frames :1127
# 3 frames :554
# 4 frames :272
# 5 frames :169
# 6 frames :1205
# 7 frames :605
# 8 frames :627
# 9 frames :403
# 10 frames :281
# 11 frames :220
# 12 frames :155
# 13 frames :166
# 14 frames :228
# 15 frames :215
# 16 frames :181
# 17 frames :130
# 18 frames :122
# 19 frames :112
# 20 frames :105
# 21 frames :119
# 22 frames :106
# 23 frames :71
# 24 frames :78
# 25 frames :69
# 26 frames :62
# 27 frames :57
# 28 frames :49
# 29 frames :48
# 30 frames :56
# 31 frames :43
# 32 frames :41
# 33 frames :31
# 34 frames :33
# 35 frames :25
# 36 frames :24
# 37 frames :32
# 38 frames :15
# 39 frames :28
# 40 frames :15
# 41 frames :15
# 42 frames :21
# 43 frames :17
# 44 frames :19
# 45 frames :13
# 46 frames :20
# 47 frames :10
# 48 frames :10
# 49 frames :11
# 50 frames :10
# 51 frames :11
# 52 frames :14
# 53 frames :8
# 54 frames :8
# 55 frames :7
# 56 frames :4
# 57 frames :5
# 58 frames :4
# 59 frames :2
# 60 frames :4
# 61 frames :1
# 62 frames :6
# 63 frames :2
# 64 frames :4
# 65 frames :1
# 67 frames :1
# 69 frames :1
# 70 frames :2
# 72 frames :2
# 73 frames :2
# 76 frames :2
# 77 frames :1
# 78 frames :1
# 79 frames :1
# 82 frames :1
# 84 frames :1
# 85 frames :3
# 89 frames :1
# 93 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.3046
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0