Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_12.09.34 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "bc6956bb1c10ecc73cb8041e8484f16d",
"name": "M1_20170603_1616_dsRNA_C5_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "10:09:55"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8048 (max 8049) in '8048'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.3237
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.66998e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 42 mean_circ: 0.94964
Ran for 1550 frames Cell Events: 61 mean_circ: 0.94799
Ran for 2050 frames Cell Events: 84 mean_circ: 0.947693
Ran for 3050 frames Cell Events: 123 mean_circ: 0.94846
Ran for 5050 frames Cell Events: 221 mean_circ: 0.949856
Ran for 5550 frames Cell Events: 249 mean_circ: 0.949829
Ran for 7050 frames Cell Events: 350 mean_circ: 0.950223
Ran for 8050 frames Cell Events: 397 mean_circ: 0.949098
Ran for 8550 frames Cell Events: 416 mean_circ: 0.949418
Ran for 9050 frames Cell Events: 439 mean_circ: 0.949266
Ran for 9550 frames Cell Events: 451 mean_circ: 0.949276
Ran for 11550 frames Cell Events: 533 mean_circ: 0.948645
Ran for 12050 frames Cell Events: 557 mean_circ: 0.949009
Ran for 15050 frames Cell Events: 669 mean_circ: 0.949653
Ran for 15550 frames Cell Events: 686 mean_circ: 0.949826
Ran for 16050 frames Cell Events: 702 mean_circ: 0.949584
Ran for 17050 frames Cell Events: 766 mean_circ: 0.949561
Ran for 17550 frames Cell Events: 785 mean_circ: 0.949634
Ran for 18050 frames Cell Events: 811 mean_circ: 0.949495
Ran for 20550 frames Cell Events: 923 mean_circ: 0.949069
Ran for 22550 frames Cell Events: 1002 mean_circ: 0.949402
Ran for 23550 frames Cell Events: 1042 mean_circ: 0.949212
Ran for 24050 frames Cell Events: 1066 mean_circ: 0.949156
Ran for 24550 frames Cell Events: 1079 mean_circ: 0.949082
Ran for 26050 frames Cell Events: 1144 mean_circ: 0.949231
Ran for 27050 frames Cell Events: 1193 mean_circ: 0.94941
Ran for 28050 frames Cell Events: 1228 mean_circ: 0.94942
Ran for 28550 frames Cell Events: 1244 mean_circ: 0.949457
Ran for 29050 frames Cell Events: 1268 mean_circ: 0.949407
Ran for 30550 frames Cell Events: 1338 mean_circ: 0.949697
Ran for 31050 frames Cell Events: 1355 mean_circ: 0.949675
Ran for 32050 frames Cell Events: 1389 mean_circ: 0.949553
Ran for 33050 frames Cell Events: 1423 mean_circ: 0.949552
Ran for 33550 frames Cell Events: 1442 mean_circ: 0.949538
Ran for 34050 frames Cell Events: 1459 mean_circ: 0.949695
Ran for 35050 frames Cell Events: 1504 mean_circ: 0.949636
Ran for 36050 frames Cell Events: 1554 mean_circ: 0.949596
Ran for 37550 frames Cell Events: 1612 mean_circ: 0.949619
Ran for 38050 frames Cell Events: 1627 mean_circ: 0.949575
Ran for 39050 frames Cell Events: 1674 mean_circ: 0.949812
Ran for 39550 frames Cell Events: 1697 mean_circ: 0.949728
Ran for 40550 frames Cell Events: 1731 mean_circ: 0.949666
Ran for 41050 frames Cell Events: 1744 mean_circ: 0.949625
Ran for 41550 frames Cell Events: 1765 mean_circ: 0.949691
Ran for 42050 frames Cell Events: 1783 mean_circ: 0.949702
Ran for 42550 frames Cell Events: 1807 mean_circ: 0.949482
Ran for 43050 frames Cell Events: 1828 mean_circ: 0.949465
Ran for 44550 frames Cell Events: 1908 mean_circ: 0.949536
Ran for 47050 frames Cell Events: 2029 mean_circ: 0.949355
Ran for 47550 frames Cell Events: 2051 mean_circ: 0.949359
Ran for 49050 frames Cell Events: 2127 mean_circ: 0.949354
Ran for 51050 frames Cell Events: 2236 mean_circ: 0.949168
Ran for 51550 frames Cell Events: 2249 mean_circ: 0.949113
Ran for 52050 frames Cell Events: 2266 mean_circ: 0.948954
Ran for 53050 frames Cell Events: 2311 mean_circ: 0.948984
Ran for 54050 frames Cell Events: 2354 mean_circ: 0.94908
Ran for 55550 frames Cell Events: 2418 mean_circ: 0.949081
Ran for 57050 frames Cell Events: 2496 mean_circ: 0.949075
Ran for 58050 frames Cell Events: 2529 mean_circ: 0.94898
Ran for 59550 frames Cell Events: 2587 mean_circ: 0.949025
Ran for 60050 frames Cell Events: 2607 mean_circ: 0.949013
Ran for 60550 frames Cell Events: 2635 mean_circ: 0.94897
Ran for 61050 frames Cell Events: 2662 mean_circ: 0.949041
Ran for 61550 frames Cell Events: 2681 mean_circ: 0.949044
Ran for 62550 frames Cell Events: 2732 mean_circ: 0.948985
Ran for 63050 frames Cell Events: 2750 mean_circ: 0.948981
Ran for 63550 frames Cell Events: 2771 mean_circ: 0.949044
Ran for 65550 frames Cell Events: 2846 mean_circ: 0.94903
Ran for 66550 frames Cell Events: 2877 mean_circ: 0.949068
Ran for 67550 frames Cell Events: 2927 mean_circ: 0.949076
Ran for 68050 frames Cell Events: 2958 mean_circ: 0.949119
Ran for 68550 frames Cell Events: 2986 mean_circ: 0.9491
Ran for 69550 frames Cell Events: 3036 mean_circ: 0.949155
Ran for 70050 frames Cell Events: 3062 mean_circ: 0.949086
Ran for 71550 frames Cell Events: 3124 mean_circ: 0.949031
Ran for 72050 frames Cell Events: 3156 mean_circ: 0.94901
Ran for 73050 frames Cell Events: 3189 mean_circ: 0.948888
Ran for 74550 frames Cell Events: 3258 mean_circ: 0.948819
Ran for 75050 frames Cell Events: 3274 mean_circ: 0.948824
Ran for 75550 frames Cell Events: 3295 mean_circ: 0.948816
Ran for 77050 frames Cell Events: 3421 mean_circ: 0.948698
Ran for 78550 frames Cell Events: 3531 mean_circ: 0.948419
Ran for 81550 frames Cell Events: 3777 mean_circ: 0.948317
Ran for 83050 frames Cell Events: 3852 mean_circ: 0.948289
Ran for 84550 frames Cell Events: 3945 mean_circ: 0.948255
Ran for 85050 frames Cell Events: 3977 mean_circ: 0.948255
Ran for 85550 frames Cell Events: 3996 mean_circ: 0.94835
Ran for 87050 frames Cell Events: 4072 mean_circ: 0.948456
Ran for 87550 frames Cell Events: 4097 mean_circ: 0.948443
Ran for 88050 frames Cell Events: 4114 mean_circ: 0.948444
Ran for 89050 frames Cell Events: 4168 mean_circ: 0.948468
Ran for 89550 frames Cell Events: 4187 mean_circ: 0.948417
Ran for 92050 frames Cell Events: 4293 mean_circ: 0.948465
Ran for 92550 frames Cell Events: 4318 mean_circ: 0.948456
Ran for 93050 frames Cell Events: 4347 mean_circ: 0.948428
Ran for 95550 frames Cell Events: 4473 mean_circ: 0.948474
Ran for 96050 frames Cell Events: 4497 mean_circ: 0.948462
Ran for 96550 frames Cell Events: 4526 mean_circ: 0.948429
Ran for 98050 frames Cell Events: 4618 mean_circ: 0.94849
Ran for 99050 frames Cell Events: 4668 mean_circ: 0.948514
Ran for 100550 frames Cell Events: 4761 mean_circ: 0.948487
Ran for 101050 frames Cell Events: 4786 mean_circ: 0.948501
Ran for 102550 frames Cell Events: 4912 mean_circ: 0.94848
Ran for 104550 frames Cell Events: 5082 mean_circ: 0.948563
Ran for 105050 frames Cell Events: 5114 mean_circ: 0.9486
Ran for 106550 frames Cell Events: 5208 mean_circ: 0.948694
Ran for 107050 frames Cell Events: 5233 mean_circ: 0.948745
Ran for 111050 frames Cell Events: 5456 mean_circ: 0.94864
Ran for 112050 frames Cell Events: 5508 mean_circ: 0.948605
Ran for 112550 frames Cell Events: 5535 mean_circ: 0.948586
Ran for 113050 frames Cell Events: 5563 mean_circ: 0.948611
Ran for 113550 frames Cell Events: 5591 mean_circ: 0.948611
Ran for 114550 frames Cell Events: 5659 mean_circ: 0.948645
Ran for 116050 frames Cell Events: 5774 mean_circ: 0.948626
Ran for 116550 frames Cell Events: 5798 mean_circ: 0.948636
Ran for 117050 frames Cell Events: 5829 mean_circ: 0.948583
Ran for 117550 frames Cell Events: 5860 mean_circ: 0.948576
Ran for 121050 frames Cell Events: 6117 mean_circ: 0.948488
Ran for 121550 frames Cell Events: 6147 mean_circ: 0.948508
Ran for 122050 frames Cell Events: 6175 mean_circ: 0.94852
Ran for 123050 frames Cell Events: 6233 mean_circ: 0.948504
Ran for 123550 frames Cell Events: 6262 mean_circ: 0.948503
Ran for 127550 frames Cell Events: 6475 mean_circ: 0.948476
Ran for 130050 frames Cell Events: 6638 mean_circ: 0.948389
Ran for 130550 frames Cell Events: 6657 mean_circ: 0.948441
Ran for 131050 frames Cell Events: 6681 mean_circ: 0.948441
Ran for 133050 frames Cell Events: 6803 mean_circ: 0.948496
Ran for 133550 frames Cell Events: 6836 mean_circ: 0.948498
Ran for 134050 frames Cell Events: 6855 mean_circ: 0.948498
Ran for 134550 frames Cell Events: 6877 mean_circ: 0.948506
Ran for 135550 frames Cell Events: 6930 mean_circ: 0.948527
Ran for 136050 frames Cell Events: 6953 mean_circ: 0.948516
Ran for 138050 frames Cell Events: 7082 mean_circ: 0.948466
Ran for 138550 frames Cell Events: 7112 mean_circ: 0.948457
Ran for 140050 frames Cell Events: 7194 mean_circ: 0.948432
Ran for 140550 frames Cell Events: 7221 mean_circ: 0.948488
Ran for 141050 frames Cell Events: 7244 mean_circ: 0.948496
Ran for 141550 frames Cell Events: 7263 mean_circ: 0.948474
Ran for 142050 frames Cell Events: 7295 mean_circ: 0.948492
Ran for 145050 frames Cell Events: 7471 mean_circ: 0.948567
Ran for 145550 frames Cell Events: 7494 mean_circ: 0.94857
Ran for 148050 frames Cell Events: 7652 mean_circ: 0.948515
Ran for 148550 frames Cell Events: 7683 mean_circ: 0.948499
Ran for 149050 frames Cell Events: 7720 mean_circ: 0.948485
Ran for 150550 frames Cell Events: 7807 mean_circ: 0.948534
Ran for 151550 frames Cell Events: 7866 mean_circ: 0.948574
Ran for 152050 frames Cell Events: 7888 mean_circ: 0.948578
Ran for 152550 frames Cell Events: 7905 mean_circ: 0.94858
Ran for 153050 frames Cell Events: 7935 mean_circ: 0.948586
Ran for 154550 frames Cell Events: 7996 mean_circ: 0.948611
Ran for 155050 frames Cell Events: 8017 mean_circ: 0.948633



End this measurment..



Stopping time: 1.67006e+009
****************************************
Measurement finished

Mean Circularitiy: 0.948629
Mean Area: 635.22
Mean FL-1 intensity: 3660.57
Mean FL-2 intensity: 3287.3
Mean FL-3 intensity: 0

frames processed: 153981
Total Time: 77961.7ms
Number of Runs (new bg img) 0
Frames collected: 155747
Skipped frames: 48753
Rescued Frames: 1516227
Samples DAQ: 38936694
Samples written: 77873250
Samples per Channel: 38936625
FPS: 1997.74

Samples per second: 499433
Expected samples/s: 500000

Events: 8049
Events per second: 103.243
jumped back (PMT): 3345
#Frames read, frequency
# 0 frames :243341
# 1 frames :55410
# 2 frames :3121
# 3 frames :1100
# 4 frames :717
# 5 frames :364
# 6 frames :1081
# 7 frames :1236
# 8 frames :836
# 9 frames :599
# 10 frames :593
# 11 frames :499
# 12 frames :393
# 13 frames :266
# 14 frames :235
# 15 frames :271
# 16 frames :194
# 17 frames :181
# 18 frames :134
# 19 frames :131
# 20 frames :91
# 21 frames :86
# 22 frames :58
# 23 frames :54
# 24 frames :46
# 25 frames :55
# 26 frames :45
# 27 frames :27
# 28 frames :39
# 29 frames :25
# 30 frames :25
# 31 frames :19
# 32 frames :28
# 33 frames :12
# 34 frames :16
# 35 frames :8
# 36 frames :16
# 37 frames :9
# 38 frames :10
# 39 frames :7
# 40 frames :9
# 41 frames :5
# 42 frames :3
# 43 frames :3
# 44 frames :8
# 45 frames :3
# 46 frames :2
# 47 frames :2
# 48 frames :2
# 49 frames :1
# 50 frames :2
# 51 frames :5
# 52 frames :5
# 53 frames :3
# 54 frames :1
# 55 frames :4
# 56 frames :2
# 58 frames :3
# 59 frames :1
# 60 frames :1
# 64 frames :1
# 65 frames :1
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# 69 frames :1
# 73 frames :1
# 75 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.3237
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0