Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_12.10.02 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "5e6af8231888f073032a32d7c41768dd",
"name": "M1_20170603_1618_dsRNA_C5_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "10:10:23"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8554 (max 8555) in '8554'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.333
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.67015e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 49 mean_circ: 0.930844
Ran for 1550 frames Cell Events: 80 mean_circ: 0.932292
Ran for 2550 frames Cell Events: 144 mean_circ: 0.936712
Ran for 3050 frames Cell Events: 167 mean_circ: 0.937403
Ran for 3550 frames Cell Events: 191 mean_circ: 0.93839
Ran for 5050 frames Cell Events: 253 mean_circ: 0.940105
Ran for 6550 frames Cell Events: 336 mean_circ: 0.941201
Ran for 7050 frames Cell Events: 364 mean_circ: 0.941295
Ran for 7550 frames Cell Events: 396 mean_circ: 0.940807
Ran for 9050 frames Cell Events: 485 mean_circ: 0.94083
Ran for 9550 frames Cell Events: 528 mean_circ: 0.939941
Ran for 12050 frames Cell Events: 704 mean_circ: 0.939694
Ran for 13550 frames Cell Events: 789 mean_circ: 0.939335
Ran for 14550 frames Cell Events: 852 mean_circ: 0.939152
Ran for 15050 frames Cell Events: 880 mean_circ: 0.939429
Ran for 16050 frames Cell Events: 949 mean_circ: 0.939433
Ran for 16550 frames Cell Events: 971 mean_circ: 0.939476
Ran for 17050 frames Cell Events: 995 mean_circ: 0.939163
Ran for 17550 frames Cell Events: 1031 mean_circ: 0.939134
Ran for 18050 frames Cell Events: 1054 mean_circ: 0.938712
Ran for 19050 frames Cell Events: 1101 mean_circ: 0.938901
Ran for 19550 frames Cell Events: 1128 mean_circ: 0.93864
Ran for 21050 frames Cell Events: 1232 mean_circ: 0.9388
Ran for 21550 frames Cell Events: 1255 mean_circ: 0.938734
Ran for 22050 frames Cell Events: 1276 mean_circ: 0.938638
Ran for 25050 frames Cell Events: 1461 mean_circ: 0.93865
Ran for 26050 frames Cell Events: 1535 mean_circ: 0.938677
Ran for 26550 frames Cell Events: 1559 mean_circ: 0.938459
Ran for 27050 frames Cell Events: 1588 mean_circ: 0.938524
Ran for 27550 frames Cell Events: 1625 mean_circ: 0.938392
Ran for 28550 frames Cell Events: 1693 mean_circ: 0.938227
Ran for 29050 frames Cell Events: 1734 mean_circ: 0.938268
Ran for 29550 frames Cell Events: 1757 mean_circ: 0.938406
Ran for 31050 frames Cell Events: 1861 mean_circ: 0.938311
Ran for 33050 frames Cell Events: 2018 mean_circ: 0.938275
Ran for 33550 frames Cell Events: 2036 mean_circ: 0.938256
Ran for 34550 frames Cell Events: 2108 mean_circ: 0.938169
Ran for 35550 frames Cell Events: 2175 mean_circ: 0.938254
Ran for 36550 frames Cell Events: 2225 mean_circ: 0.938268
Ran for 37050 frames Cell Events: 2271 mean_circ: 0.938305
Ran for 39550 frames Cell Events: 2440 mean_circ: 0.938192
Ran for 42050 frames Cell Events: 2561 mean_circ: 0.93802
Ran for 42550 frames Cell Events: 2585 mean_circ: 0.938039
Ran for 44050 frames Cell Events: 2690 mean_circ: 0.938178
Ran for 45050 frames Cell Events: 2762 mean_circ: 0.938078
Ran for 46550 frames Cell Events: 2877 mean_circ: 0.937922
Ran for 47050 frames Cell Events: 2915 mean_circ: 0.937929
Ran for 49050 frames Cell Events: 3069 mean_circ: 0.938088
Ran for 50550 frames Cell Events: 3187 mean_circ: 0.937909
Ran for 51050 frames Cell Events: 3218 mean_circ: 0.937846
Ran for 51550 frames Cell Events: 3260 mean_circ: 0.937884
Ran for 53050 frames Cell Events: 3348 mean_circ: 0.937789
Ran for 57050 frames Cell Events: 3623 mean_circ: 0.938129
Ran for 58050 frames Cell Events: 3689 mean_circ: 0.938026
Ran for 58550 frames Cell Events: 3728 mean_circ: 0.938017
Ran for 59050 frames Cell Events: 3761 mean_circ: 0.938076
Ran for 59550 frames Cell Events: 3791 mean_circ: 0.9381
Ran for 61550 frames Cell Events: 3886 mean_circ: 0.938201
Ran for 62050 frames Cell Events: 3921 mean_circ: 0.938245
Ran for 62550 frames Cell Events: 3940 mean_circ: 0.93824
Ran for 63050 frames Cell Events: 3967 mean_circ: 0.93818
Ran for 64050 frames Cell Events: 4037 mean_circ: 0.93827
Ran for 66050 frames Cell Events: 4136 mean_circ: 0.938335
Ran for 67550 frames Cell Events: 4232 mean_circ: 0.938312
Ran for 68550 frames Cell Events: 4292 mean_circ: 0.938374
Ran for 69050 frames Cell Events: 4324 mean_circ: 0.938445
Ran for 69550 frames Cell Events: 4375 mean_circ: 0.938471
Ran for 72050 frames Cell Events: 4517 mean_circ: 0.938514
Ran for 72550 frames Cell Events: 4558 mean_circ: 0.93859
Ran for 73050 frames Cell Events: 4588 mean_circ: 0.938651
Ran for 73550 frames Cell Events: 4607 mean_circ: 0.938642
Ran for 74050 frames Cell Events: 4638 mean_circ: 0.938705
Ran for 74550 frames Cell Events: 4662 mean_circ: 0.938673
Ran for 75050 frames Cell Events: 4701 mean_circ: 0.938603
Ran for 75550 frames Cell Events: 4727 mean_circ: 0.938564
Ran for 76550 frames Cell Events: 4772 mean_circ: 0.938561
Ran for 77550 frames Cell Events: 4842 mean_circ: 0.938575
Ran for 79050 frames Cell Events: 4936 mean_circ: 0.938556
Ran for 80050 frames Cell Events: 4982 mean_circ: 0.938592
Ran for 83050 frames Cell Events: 5212 mean_circ: 0.938598
Ran for 85550 frames Cell Events: 5349 mean_circ: 0.938506
Ran for 86050 frames Cell Events: 5380 mean_circ: 0.938559
Ran for 86550 frames Cell Events: 5407 mean_circ: 0.938565
Ran for 89550 frames Cell Events: 5595 mean_circ: 0.938457
Ran for 90550 frames Cell Events: 5688 mean_circ: 0.938535
Ran for 91050 frames Cell Events: 5722 mean_circ: 0.938509
Ran for 92050 frames Cell Events: 5769 mean_circ: 0.938561
Ran for 93550 frames Cell Events: 5876 mean_circ: 0.938625
Ran for 94550 frames Cell Events: 5962 mean_circ: 0.938637
Ran for 95050 frames Cell Events: 5989 mean_circ: 0.938648
Ran for 96050 frames Cell Events: 6047 mean_circ: 0.93866
Ran for 97050 frames Cell Events: 6118 mean_circ: 0.938714
Ran for 97550 frames Cell Events: 6154 mean_circ: 0.938722
Ran for 98050 frames Cell Events: 6183 mean_circ: 0.938739
Ran for 100050 frames Cell Events: 6308 mean_circ: 0.93871
Ran for 100550 frames Cell Events: 6342 mean_circ: 0.938692
Ran for 102050 frames Cell Events: 6456 mean_circ: 0.938779
Ran for 103550 frames Cell Events: 6565 mean_circ: 0.938878
Ran for 104050 frames Cell Events: 6590 mean_circ: 0.938916
Ran for 105550 frames Cell Events: 6676 mean_circ: 0.938946
Ran for 106050 frames Cell Events: 6713 mean_circ: 0.938946
Ran for 106550 frames Cell Events: 6736 mean_circ: 0.938955
Ran for 108550 frames Cell Events: 6857 mean_circ: 0.938952
Ran for 110050 frames Cell Events: 6959 mean_circ: 0.938911
Ran for 111550 frames Cell Events: 7047 mean_circ: 0.938909
Ran for 112050 frames Cell Events: 7074 mean_circ: 0.938851
Ran for 112550 frames Cell Events: 7108 mean_circ: 0.938836
Ran for 113050 frames Cell Events: 7147 mean_circ: 0.938836
Ran for 114050 frames Cell Events: 7209 mean_circ: 0.938873
Ran for 114550 frames Cell Events: 7245 mean_circ: 0.938868
Ran for 115550 frames Cell Events: 7304 mean_circ: 0.93894
Ran for 117050 frames Cell Events: 7389 mean_circ: 0.938827
Ran for 117550 frames Cell Events: 7422 mean_circ: 0.938796
Ran for 118050 frames Cell Events: 7448 mean_circ: 0.938785
Ran for 119550 frames Cell Events: 7557 mean_circ: 0.938826
Ran for 121050 frames Cell Events: 7651 mean_circ: 0.938795
Ran for 122050 frames Cell Events: 7695 mean_circ: 0.938734
Ran for 122550 frames Cell Events: 7731 mean_circ: 0.938734
Ran for 123050 frames Cell Events: 7759 mean_circ: 0.938758
Ran for 124050 frames Cell Events: 7853 mean_circ: 0.938713
Ran for 126050 frames Cell Events: 7991 mean_circ: 0.938724
Ran for 126550 frames Cell Events: 8042 mean_circ: 0.938724
Ran for 127550 frames Cell Events: 8105 mean_circ: 0.938734
Ran for 128050 frames Cell Events: 8135 mean_circ: 0.93874
Ran for 129050 frames Cell Events: 8203 mean_circ: 0.938728
Ran for 130550 frames Cell Events: 8256 mean_circ: 0.938757
Ran for 133050 frames Cell Events: 8434 mean_circ: 0.938849
Ran for 134550 frames Cell Events: 8538 mean_circ: 0.938804



End this measurment..



Stopping time: 1.67022e+009
****************************************
Measurement finished

Mean Circularitiy: 0.93877
Mean Area: 636.534
Mean FL-1 intensity: 3191.49
Mean FL-2 intensity: 3395.84
Mean FL-3 intensity: 0

frames processed: 131542
Total Time: 67425.5ms
Number of Runs (new bg img) 0
Frames collected: 134690
Skipped frames: 51901
Rescued Frames: 1591291
Samples DAQ: 33674317
Samples written: 67344714
Samples per Channel: 33672357
FPS: 1997.61

Samples per second: 499401
Expected samples/s: 500000

Events: 8555
Events per second: 126.881
jumped back (PMT): 26
#Frames read, frequency
# 0 frames :212486
# 1 frames :45035
# 2 frames :2390
# 3 frames :1007
# 4 frames :470
# 5 frames :209
# 6 frames :2157
# 7 frames :1072
# 8 frames :940
# 9 frames :593
# 10 frames :396
# 11 frames :310
# 12 frames :225
# 13 frames :200
# 14 frames :260
# 15 frames :180
# 16 frames :157
# 17 frames :123
# 18 frames :80
# 19 frames :78
# 20 frames :79
# 21 frames :72
# 22 frames :57
# 23 frames :51
# 24 frames :38
# 25 frames :37
# 26 frames :33
# 27 frames :32
# 28 frames :19
# 29 frames :23
# 30 frames :21
# 31 frames :10
# 32 frames :18
# 33 frames :11
# 34 frames :9
# 35 frames :13
# 36 frames :7
# 37 frames :5
# 38 frames :10
# 39 frames :7
# 40 frames :6
# 41 frames :10
# 42 frames :4
# 43 frames :3
# 44 frames :2
# 45 frames :5
# 46 frames :3
# 47 frames :1
# 48 frames :3
# 49 frames :3
# 50 frames :4
# 51 frames :2
# 52 frames :2
# 53 frames :1
# 54 frames :1
# 55 frames :2
# 57 frames :2
# 59 frames :2
# 60 frames :1
# 61 frames :3
# 68 frames :1
# 78 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.333
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0