Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_12.10.30 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "9d3b9b10a19dec75547c25c3cfab3423",
"name": "M1_20170603_1626_dsRNA_b7_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "10:10:54"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 9446 (max 9447) in '9446'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.3571
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.67064e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 28 mean_circ: 0.953956
Ran for 3550 frames Cell Events: 206 mean_circ: 0.946449
Ran for 4050 frames Cell Events: 251 mean_circ: 0.94742
Ran for 5550 frames Cell Events: 324 mean_circ: 0.945971
Ran for 8550 frames Cell Events: 515 mean_circ: 0.945392
Ran for 9050 frames Cell Events: 546 mean_circ: 0.945533
Ran for 10050 frames Cell Events: 603 mean_circ: 0.945375
Ran for 11050 frames Cell Events: 669 mean_circ: 0.945748
Ran for 11550 frames Cell Events: 697 mean_circ: 0.946038
Ran for 13550 frames Cell Events: 842 mean_circ: 0.946609
Ran for 14050 frames Cell Events: 869 mean_circ: 0.946642
Ran for 15050 frames Cell Events: 927 mean_circ: 0.94664
Ran for 16050 frames Cell Events: 996 mean_circ: 0.946698
Ran for 17550 frames Cell Events: 1086 mean_circ: 0.946602
Ran for 18550 frames Cell Events: 1148 mean_circ: 0.946422
Ran for 19550 frames Cell Events: 1201 mean_circ: 0.946629
Ran for 20050 frames Cell Events: 1231 mean_circ: 0.946561
Ran for 22050 frames Cell Events: 1333 mean_circ: 0.94603
Ran for 22550 frames Cell Events: 1363 mean_circ: 0.945913
Ran for 23550 frames Cell Events: 1426 mean_circ: 0.946041
Ran for 24050 frames Cell Events: 1444 mean_circ: 0.945937
Ran for 26550 frames Cell Events: 1602 mean_circ: 0.94627
Ran for 27050 frames Cell Events: 1617 mean_circ: 0.946221
Ran for 28050 frames Cell Events: 1668 mean_circ: 0.946324
Ran for 30050 frames Cell Events: 1789 mean_circ: 0.946303
Ran for 31550 frames Cell Events: 1894 mean_circ: 0.945798
Ran for 33050 frames Cell Events: 1989 mean_circ: 0.945985
Ran for 35550 frames Cell Events: 2164 mean_circ: 0.945941
Ran for 38050 frames Cell Events: 2338 mean_circ: 0.945635
Ran for 39550 frames Cell Events: 2440 mean_circ: 0.945569
Ran for 40050 frames Cell Events: 2470 mean_circ: 0.945551
Ran for 41550 frames Cell Events: 2569 mean_circ: 0.945622
Ran for 43550 frames Cell Events: 2727 mean_circ: 0.945626
Ran for 44050 frames Cell Events: 2761 mean_circ: 0.945784
Ran for 45050 frames Cell Events: 2840 mean_circ: 0.945709
Ran for 46050 frames Cell Events: 2907 mean_circ: 0.945822
Ran for 47050 frames Cell Events: 2986 mean_circ: 0.94604
Ran for 47550 frames Cell Events: 3008 mean_circ: 0.946164
Ran for 48550 frames Cell Events: 3077 mean_circ: 0.946135
Ran for 50550 frames Cell Events: 3214 mean_circ: 0.946298
Ran for 51550 frames Cell Events: 3276 mean_circ: 0.946112
Ran for 52050 frames Cell Events: 3304 mean_circ: 0.946057
Ran for 53050 frames Cell Events: 3368 mean_circ: 0.946067
Ran for 54050 frames Cell Events: 3436 mean_circ: 0.946115
Ran for 54550 frames Cell Events: 3469 mean_circ: 0.946147
Ran for 58550 frames Cell Events: 3704 mean_circ: 0.946229
Ran for 59550 frames Cell Events: 3780 mean_circ: 0.946274
Ran for 60050 frames Cell Events: 3809 mean_circ: 0.946235
Ran for 61050 frames Cell Events: 3885 mean_circ: 0.946252
Ran for 62050 frames Cell Events: 3948 mean_circ: 0.946196
Ran for 64050 frames Cell Events: 4090 mean_circ: 0.946337
Ran for 64550 frames Cell Events: 4122 mean_circ: 0.946349
Ran for 65550 frames Cell Events: 4169 mean_circ: 0.946199
Ran for 66550 frames Cell Events: 4227 mean_circ: 0.946217
Ran for 67050 frames Cell Events: 4271 mean_circ: 0.946274
Ran for 67550 frames Cell Events: 4300 mean_circ: 0.946246
Ran for 69550 frames Cell Events: 4434 mean_circ: 0.946281
Ran for 70050 frames Cell Events: 4465 mean_circ: 0.946301
Ran for 71550 frames Cell Events: 4560 mean_circ: 0.946101
Ran for 72550 frames Cell Events: 4619 mean_circ: 0.946113
Ran for 74050 frames Cell Events: 4701 mean_circ: 0.946191
Ran for 74550 frames Cell Events: 4715 mean_circ: 0.946291
Ran for 76050 frames Cell Events: 4814 mean_circ: 0.946288
Ran for 76550 frames Cell Events: 4840 mean_circ: 0.946288
Ran for 77550 frames Cell Events: 4901 mean_circ: 0.946238
Ran for 78550 frames Cell Events: 4972 mean_circ: 0.946344
Ran for 79550 frames Cell Events: 5032 mean_circ: 0.946176
Ran for 80050 frames Cell Events: 5057 mean_circ: 0.946129
Ran for 81050 frames Cell Events: 5121 mean_circ: 0.946194
Ran for 81550 frames Cell Events: 5150 mean_circ: 0.946165
Ran for 82050 frames Cell Events: 5176 mean_circ: 0.946135
Ran for 83550 frames Cell Events: 5285 mean_circ: 0.946149
Ran for 87550 frames Cell Events: 5589 mean_circ: 0.946216
Ran for 90550 frames Cell Events: 5840 mean_circ: 0.945975
Ran for 91550 frames Cell Events: 5924 mean_circ: 0.945975
Ran for 92050 frames Cell Events: 5949 mean_circ: 0.946053
Ran for 93050 frames Cell Events: 6021 mean_circ: 0.946073
Ran for 96050 frames Cell Events: 6235 mean_circ: 0.945964
Ran for 96550 frames Cell Events: 6264 mean_circ: 0.945973
Ran for 98050 frames Cell Events: 6369 mean_circ: 0.945948
Ran for 98550 frames Cell Events: 6388 mean_circ: 0.945929
Ran for 100050 frames Cell Events: 6507 mean_circ: 0.945939
Ran for 100550 frames Cell Events: 6536 mean_circ: 0.945864
Ran for 101050 frames Cell Events: 6561 mean_circ: 0.9458
Ran for 101550 frames Cell Events: 6585 mean_circ: 0.945783
Ran for 103050 frames Cell Events: 6662 mean_circ: 0.945818
Ran for 104550 frames Cell Events: 6779 mean_circ: 0.945813
Ran for 106550 frames Cell Events: 6896 mean_circ: 0.945795
Ran for 108050 frames Cell Events: 7018 mean_circ: 0.945824
Ran for 108550 frames Cell Events: 7039 mean_circ: 0.94575
Ran for 109050 frames Cell Events: 7066 mean_circ: 0.945735
Ran for 109550 frames Cell Events: 7096 mean_circ: 0.945761
Ran for 110550 frames Cell Events: 7173 mean_circ: 0.945733
Ran for 111550 frames Cell Events: 7250 mean_circ: 0.945778
Ran for 112050 frames Cell Events: 7272 mean_circ: 0.945794
Ran for 112550 frames Cell Events: 7310 mean_circ: 0.945804
Ran for 113050 frames Cell Events: 7340 mean_circ: 0.945817
Ran for 116550 frames Cell Events: 7565 mean_circ: 0.945763
Ran for 118050 frames Cell Events: 7650 mean_circ: 0.94577
Ran for 119050 frames Cell Events: 7736 mean_circ: 0.945734
Ran for 121050 frames Cell Events: 7884 mean_circ: 0.94573
Ran for 122050 frames Cell Events: 7944 mean_circ: 0.945655
Ran for 122550 frames Cell Events: 7983 mean_circ: 0.945671
Ran for 123050 frames Cell Events: 8018 mean_circ: 0.945681
Ran for 123550 frames Cell Events: 8065 mean_circ: 0.945658
Ran for 124050 frames Cell Events: 8090 mean_circ: 0.945682
Ran for 124550 frames Cell Events: 8127 mean_circ: 0.945659
Ran for 125050 frames Cell Events: 8155 mean_circ: 0.945621
Ran for 125550 frames Cell Events: 8187 mean_circ: 0.945633
Ran for 126550 frames Cell Events: 8250 mean_circ: 0.945546
Ran for 128550 frames Cell Events: 8375 mean_circ: 0.945397
Ran for 129050 frames Cell Events: 8408 mean_circ: 0.945413
Ran for 131550 frames Cell Events: 8564 mean_circ: 0.945358
Ran for 132050 frames Cell Events: 8610 mean_circ: 0.945377
Ran for 133550 frames Cell Events: 8732 mean_circ: 0.945425
Ran for 134550 frames Cell Events: 8802 mean_circ: 0.94542
Ran for 135550 frames Cell Events: 8865 mean_circ: 0.945381
Ran for 137050 frames Cell Events: 8988 mean_circ: 0.945333
Ran for 137550 frames Cell Events: 9027 mean_circ: 0.945319
Ran for 138550 frames Cell Events: 9082 mean_circ: 0.945293
Ran for 141550 frames Cell Events: 9287 mean_circ: 0.945234



End this measurment..



Stopping time: 1.67071e+009
****************************************
Measurement finished

Mean Circularitiy: 0.945225
Mean Area: 635.031
Mean FL-1 intensity: 3788.18
Mean FL-2 intensity: 3445.94
Mean FL-3 intensity: 0

frames processed: 141127
Total Time: 71799.9ms
Number of Runs (new bg img) 0
Frames collected: 143406
Skipped frames: 54180
Rescued Frames: 1681131
Samples DAQ: 35856401
Samples written: 71703058
Samples per Channel: 35851529
FPS: 1997.3

Samples per second: 499325
Expected samples/s: 500000

Events: 9447
Events per second: 131.574
jumped back (PMT): 3046
#Frames read, frequency
# 0 frames :234628
# 1 frames :39571
# 2 frames :2535
# 3 frames :952
# 4 frames :533
# 5 frames :320
# 6 frames :1406
# 7 frames :921
# 8 frames :819
# 9 frames :507
# 10 frames :603
# 11 frames :396
# 12 frames :328
# 13 frames :210
# 14 frames :258
# 15 frames :228
# 16 frames :238
# 17 frames :186
# 18 frames :166
# 19 frames :97
# 20 frames :105
# 21 frames :103
# 22 frames :97
# 23 frames :66
# 24 frames :65
# 25 frames :60
# 26 frames :60
# 27 frames :52
# 28 frames :44
# 29 frames :41
# 30 frames :36
# 31 frames :37
# 32 frames :33
# 33 frames :20
# 34 frames :21
# 35 frames :15
# 36 frames :12
# 37 frames :18
# 38 frames :28
# 39 frames :10
# 40 frames :11
# 41 frames :17
# 42 frames :11
# 43 frames :10
# 44 frames :12
# 45 frames :7
# 46 frames :6
# 47 frames :6
# 48 frames :4
# 49 frames :1
# 50 frames :5
# 51 frames :3
# 52 frames :4
# 53 frames :4
# 54 frames :6
# 55 frames :2
# 57 frames :5
# 58 frames :3
# 61 frames :2
# 62 frames :4
# 65 frames :1
# 67 frames :1
# 72 frames :1
# 75 frames :1
# 81 frames :1
# 83 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.3571
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0