Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_12.11.02 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "b79c04fe65f576e28f6d2af911f9d039",
"name": "M1_20170603_1629_dsRNA_B7_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "10:11:26"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 9999 (max 10000) in
'9999'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.37
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.6708e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 23 mean_circ: 0.930522
Ran for 1050 frames Cell Events: 57 mean_circ: 0.935902
Ran for 2050 frames Cell Events: 133 mean_circ: 0.935622
Ran for 3050 frames Cell Events: 193 mean_circ: 0.934298
Ran for 4050 frames Cell Events: 270 mean_circ: 0.935327
Ran for 4550 frames Cell Events: 303 mean_circ: 0.9353
Ran for 5550 frames Cell Events: 369 mean_circ: 0.935058
Ran for 7550 frames Cell Events: 497 mean_circ: 0.936021
Ran for 8550 frames Cell Events: 578 mean_circ: 0.936629
Ran for 10050 frames Cell Events: 688 mean_circ: 0.936923
Ran for 10550 frames Cell Events: 716 mean_circ: 0.936821
Ran for 11050 frames Cell Events: 751 mean_circ: 0.936661
Ran for 14050 frames Cell Events: 953 mean_circ: 0.936728
Ran for 15050 frames Cell Events: 1024 mean_circ: 0.936646
Ran for 15550 frames Cell Events: 1060 mean_circ: 0.936577
Ran for 16550 frames Cell Events: 1121 mean_circ: 0.936318
Ran for 17050 frames Cell Events: 1157 mean_circ: 0.936063
Ran for 17550 frames Cell Events: 1196 mean_circ: 0.93618
Ran for 18550 frames Cell Events: 1260 mean_circ: 0.936061
Ran for 20550 frames Cell Events: 1429 mean_circ: 0.935475
Ran for 21050 frames Cell Events: 1467 mean_circ: 0.935919
Ran for 21550 frames Cell Events: 1507 mean_circ: 0.935693
Ran for 23550 frames Cell Events: 1676 mean_circ: 0.935458
Ran for 26550 frames Cell Events: 1924 mean_circ: 0.935194
Ran for 27050 frames Cell Events: 1971 mean_circ: 0.935321
Ran for 28050 frames Cell Events: 2050 mean_circ: 0.935428
Ran for 29050 frames Cell Events: 2138 mean_circ: 0.935347
Ran for 29550 frames Cell Events: 2177 mean_circ: 0.935397
Ran for 33050 frames Cell Events: 2433 mean_circ: 0.935016
Ran for 33550 frames Cell Events: 2472 mean_circ: 0.935016
Ran for 34050 frames Cell Events: 2515 mean_circ: 0.935131
Ran for 34550 frames Cell Events: 2561 mean_circ: 0.935118
Ran for 36550 frames Cell Events: 2710 mean_circ: 0.935063
Ran for 37050 frames Cell Events: 2758 mean_circ: 0.935061
Ran for 38050 frames Cell Events: 2817 mean_circ: 0.93504
Ran for 38550 frames Cell Events: 2861 mean_circ: 0.935056
Ran for 41050 frames Cell Events: 3083 mean_circ: 0.93486
Ran for 44050 frames Cell Events: 3325 mean_circ: 0.935031
Ran for 44550 frames Cell Events: 3365 mean_circ: 0.934951
Ran for 45550 frames Cell Events: 3426 mean_circ: 0.934968
Ran for 46050 frames Cell Events: 3452 mean_circ: 0.934915
Ran for 47550 frames Cell Events: 3538 mean_circ: 0.934871
Ran for 48050 frames Cell Events: 3576 mean_circ: 0.934762
Ran for 49050 frames Cell Events: 3654 mean_circ: 0.93466
Ran for 51050 frames Cell Events: 3790 mean_circ: 0.934684
Ran for 52050 frames Cell Events: 3820 mean_circ: 0.934752
Ran for 52550 frames Cell Events: 3848 mean_circ: 0.934655
Ran for 54550 frames Cell Events: 4016 mean_circ: 0.934685
Ran for 55550 frames Cell Events: 4090 mean_circ: 0.934841
Ran for 58050 frames Cell Events: 4263 mean_circ: 0.934753
Ran for 58550 frames Cell Events: 4306 mean_circ: 0.93468
Ran for 59050 frames Cell Events: 4339 mean_circ: 0.934416
Ran for 59550 frames Cell Events: 4370 mean_circ: 0.934344
Ran for 60550 frames Cell Events: 4430 mean_circ: 0.934273
Ran for 63050 frames Cell Events: 4600 mean_circ: 0.934129
Ran for 64050 frames Cell Events: 4674 mean_circ: 0.93418
Ran for 66550 frames Cell Events: 4886 mean_circ: 0.934256
Ran for 67550 frames Cell Events: 4957 mean_circ: 0.93425
Ran for 70050 frames Cell Events: 5162 mean_circ: 0.934097
Ran for 72050 frames Cell Events: 5310 mean_circ: 0.933994
Ran for 73050 frames Cell Events: 5381 mean_circ: 0.933989
Ran for 73550 frames Cell Events: 5421 mean_circ: 0.934011
Ran for 74050 frames Cell Events: 5479 mean_circ: 0.933977
Ran for 74550 frames Cell Events: 5517 mean_circ: 0.934028
Ran for 76050 frames Cell Events: 5635 mean_circ: 0.934044
Ran for 76550 frames Cell Events: 5663 mean_circ: 0.934032
Ran for 77050 frames Cell Events: 5708 mean_circ: 0.934056
Ran for 78050 frames Cell Events: 5775 mean_circ: 0.934057
Ran for 78550 frames Cell Events: 5817 mean_circ: 0.93401
Ran for 80550 frames Cell Events: 5982 mean_circ: 0.933907
Ran for 81050 frames Cell Events: 6031 mean_circ: 0.933907
Ran for 82550 frames Cell Events: 6167 mean_circ: 0.933902
Ran for 84050 frames Cell Events: 6277 mean_circ: 0.93397
Ran for 86050 frames Cell Events: 6407 mean_circ: 0.933844
Ran for 86550 frames Cell Events: 6452 mean_circ: 0.933876
Ran for 91050 frames Cell Events: 6831 mean_circ: 0.93376
Ran for 92050 frames Cell Events: 6919 mean_circ: 0.933798
Ran for 92550 frames Cell Events: 6946 mean_circ: 0.933787
Ran for 95050 frames Cell Events: 7177 mean_circ: 0.933741
Ran for 96050 frames Cell Events: 7252 mean_circ: 0.933762
Ran for 96550 frames Cell Events: 7287 mean_circ: 0.933737
Ran for 97050 frames Cell Events: 7326 mean_circ: 0.933773
Ran for 97550 frames Cell Events: 7369 mean_circ: 0.933746
Ran for 98550 frames Cell Events: 7453 mean_circ: 0.933674
Ran for 99050 frames Cell Events: 7489 mean_circ: 0.933624
Ran for 99550 frames Cell Events: 7520 mean_circ: 0.933582
Ran for 101550 frames Cell Events: 7674 mean_circ: 0.933552
Ran for 102550 frames Cell Events: 7750 mean_circ: 0.933543
Ran for 103050 frames Cell Events: 7781 mean_circ: 0.933552
Ran for 104550 frames Cell Events: 7904 mean_circ: 0.93354
Ran for 106550 frames Cell Events: 8070 mean_circ: 0.933564
Ran for 108050 frames Cell Events: 8183 mean_circ: 0.933637
Ran for 108550 frames Cell Events: 8216 mean_circ: 0.93359
Ran for 111550 frames Cell Events: 8480 mean_circ: 0.933665
Ran for 113550 frames Cell Events: 8620 mean_circ: 0.933583
Ran for 114550 frames Cell Events: 8700 mean_circ: 0.933568
Ran for 115050 frames Cell Events: 8732 mean_circ: 0.933546
Ran for 117550 frames Cell Events: 8950 mean_circ: 0.933522
Ran for 118050 frames Cell Events: 8987 mean_circ: 0.933513
Ran for 119050 frames Cell Events: 9075 mean_circ: 0.933509
Ran for 120550 frames Cell Events: 9178 mean_circ: 0.933488
Ran for 121050 frames Cell Events: 9203 mean_circ: 0.9335
Ran for 121550 frames Cell Events: 9231 mean_circ: 0.933498
Ran for 122050 frames Cell Events: 9272 mean_circ: 0.933525
Ran for 122550 frames Cell Events: 9307 mean_circ: 0.933515
Ran for 123050 frames Cell Events: 9344 mean_circ: 0.933514
Ran for 123550 frames Cell Events: 9388 mean_circ: 0.933548
Ran for 125050 frames Cell Events: 9501 mean_circ: 0.933622
Ran for 125550 frames Cell Events: 9538 mean_circ: 0.933634
Ran for 127050 frames Cell Events: 9691 mean_circ: 0.933605
Ran for 128550 frames Cell Events: 9806 mean_circ: 0.933589



End this measurment.
because stop condition (time/nr of cells) was met

Stopping time: 1.67086e+009
****************************************
Measurement finished

Mean Circularitiy: 0.933608
Mean Area: 637.699
Mean FL-1 intensity: 3616.91
Mean FL-2 intensity: 3688.36
Mean FL-3 intensity: 0

frames processed: 127088
Total Time: 65467.2ms
Number of Runs (new bg img) 0
Frames collected: 130767
Skipped frames: 57859
Rescued Frames: 1764153
Samples DAQ: 32691865
Samples written: 65383648
Samples per Channel: 32691824
FPS: 1997.44

Samples per second: 499362
Expected samples/s: 500000

Events: 10000
Events per second: 152.748
jumped back (PMT): 27
#Frames read, frequency
# 0 frames :216873
# 1 frames :33326
# 2 frames :1833
# 3 frames :856
# 4 frames :396
# 5 frames :200
# 6 frames :2037
# 7 frames :962
# 8 frames :864
# 9 frames :524
# 10 frames :366
# 11 frames :270
# 12 frames :245
# 13 frames :228
# 14 frames :274
# 15 frames :221
# 16 frames :171
# 17 frames :146
# 18 frames :107
# 19 frames :120
# 20 frames :89
# 21 frames :99
# 22 frames :94
# 23 frames :77
# 24 frames :60
# 25 frames :48
# 26 frames :45
# 27 frames :42
# 28 frames :42
# 29 frames :39
# 30 frames :36
# 31 frames :34
# 32 frames :25
# 33 frames :23
# 34 frames :15
# 35 frames :22
# 36 frames :19
# 37 frames :15
# 38 frames :12
# 39 frames :13
# 40 frames :10
# 41 frames :12
# 42 frames :7
# 43 frames :6
# 44 frames :9
# 45 frames :4
# 46 frames :4
# 47 frames :3
# 48 frames :6
# 49 frames :6
# 50 frames :4
# 51 frames :2
# 52 frames :4
# 53 frames :2
# 54 frames :6
# 55 frames :3
# 58 frames :3
# 59 frames :2
# 60 frames :1
# 61 frames :2
# 62 frames :3
# 63 frames :1
# 65 frames :1
# 66 frames :1
# 70 frames :2
# 75 frames :1
# 89 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.37
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0