Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_12.12.31 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "a1af42b5cf606b6dc35ca9db4fedbfe3",
"name": "M1_20170603_1648_dsRNA_M3_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "10:12:52"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8217 (max 8218) in '8217'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.428
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.67192e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1550 frames Cell Events: 89 mean_circ: 0.948537
Ran for 2050 frames Cell Events: 113 mean_circ: 0.948022
Ran for 4050 frames Cell Events: 238 mean_circ: 0.949347
Ran for 5050 frames Cell Events: 302 mean_circ: 0.949256
Ran for 7050 frames Cell Events: 411 mean_circ: 0.948227
Ran for 8050 frames Cell Events: 464 mean_circ: 0.948107
Ran for 12550 frames Cell Events: 743 mean_circ: 0.948227
Ran for 13550 frames Cell Events: 802 mean_circ: 0.948129
Ran for 14050 frames Cell Events: 826 mean_circ: 0.948015
Ran for 14550 frames Cell Events: 852 mean_circ: 0.948129
Ran for 15550 frames Cell Events: 922 mean_circ: 0.947728
Ran for 16050 frames Cell Events: 935 mean_circ: 0.947619
Ran for 16550 frames Cell Events: 965 mean_circ: 0.947573
Ran for 17050 frames Cell Events: 990 mean_circ: 0.947476
Ran for 17550 frames Cell Events: 1026 mean_circ: 0.947651
Ran for 18550 frames Cell Events: 1075 mean_circ: 0.947655
Ran for 19550 frames Cell Events: 1126 mean_circ: 0.947897
Ran for 20050 frames Cell Events: 1138 mean_circ: 0.947799
Ran for 21550 frames Cell Events: 1219 mean_circ: 0.947479
Ran for 26050 frames Cell Events: 1499 mean_circ: 0.947586
Ran for 26550 frames Cell Events: 1521 mean_circ: 0.947328
Ran for 28550 frames Cell Events: 1632 mean_circ: 0.947345
Ran for 29050 frames Cell Events: 1657 mean_circ: 0.947373
Ran for 31550 frames Cell Events: 1789 mean_circ: 0.947698
Ran for 33050 frames Cell Events: 1875 mean_circ: 0.947384
Ran for 33550 frames Cell Events: 1901 mean_circ: 0.947425
Ran for 36050 frames Cell Events: 2018 mean_circ: 0.947506
Ran for 38050 frames Cell Events: 2119 mean_circ: 0.947508
Ran for 40050 frames Cell Events: 2244 mean_circ: 0.947644
Ran for 41050 frames Cell Events: 2321 mean_circ: 0.94755
Ran for 41550 frames Cell Events: 2358 mean_circ: 0.94755
Ran for 42050 frames Cell Events: 2386 mean_circ: 0.947342
Ran for 43050 frames Cell Events: 2441 mean_circ: 0.947346
Ran for 45050 frames Cell Events: 2559 mean_circ: 0.947601
Ran for 46050 frames Cell Events: 2614 mean_circ: 0.947662
Ran for 48050 frames Cell Events: 2737 mean_circ: 0.947777
Ran for 50550 frames Cell Events: 2905 mean_circ: 0.947833
Ran for 51050 frames Cell Events: 2929 mean_circ: 0.947731
Ran for 52050 frames Cell Events: 2983 mean_circ: 0.947893
Ran for 53050 frames Cell Events: 3036 mean_circ: 0.947957
Ran for 55550 frames Cell Events: 3178 mean_circ: 0.947896
Ran for 56550 frames Cell Events: 3222 mean_circ: 0.947939
Ran for 57050 frames Cell Events: 3255 mean_circ: 0.947919
Ran for 58550 frames Cell Events: 3344 mean_circ: 0.947958
Ran for 60050 frames Cell Events: 3408 mean_circ: 0.947928
Ran for 60550 frames Cell Events: 3439 mean_circ: 0.947928
Ran for 61050 frames Cell Events: 3462 mean_circ: 0.947838
Ran for 61550 frames Cell Events: 3492 mean_circ: 0.947838
Ran for 62050 frames Cell Events: 3526 mean_circ: 0.947886
Ran for 63050 frames Cell Events: 3590 mean_circ: 0.947873
Ran for 64050 frames Cell Events: 3644 mean_circ: 0.947934
Ran for 65550 frames Cell Events: 3748 mean_circ: 0.948
Ran for 66050 frames Cell Events: 3782 mean_circ: 0.947996
Ran for 68050 frames Cell Events: 3923 mean_circ: 0.948089
Ran for 69050 frames Cell Events: 3978 mean_circ: 0.947998
Ran for 69550 frames Cell Events: 4004 mean_circ: 0.947995
Ran for 70550 frames Cell Events: 4068 mean_circ: 0.948008
Ran for 71050 frames Cell Events: 4092 mean_circ: 0.948013
Ran for 72050 frames Cell Events: 4152 mean_circ: 0.947938
Ran for 72550 frames Cell Events: 4177 mean_circ: 0.947956
Ran for 73550 frames Cell Events: 4236 mean_circ: 0.947883
Ran for 74050 frames Cell Events: 4260 mean_circ: 0.947684
Ran for 74550 frames Cell Events: 4287 mean_circ: 0.947713
Ran for 75550 frames Cell Events: 4358 mean_circ: 0.947771
Ran for 76050 frames Cell Events: 4405 mean_circ: 0.947776
Ran for 77050 frames Cell Events: 4472 mean_circ: 0.947793
Ran for 80550 frames Cell Events: 4699 mean_circ: 0.947679
Ran for 84050 frames Cell Events: 4918 mean_circ: 0.947661
Ran for 84550 frames Cell Events: 4933 mean_circ: 0.947657
Ran for 85050 frames Cell Events: 4967 mean_circ: 0.94766
Ran for 85550 frames Cell Events: 4993 mean_circ: 0.947665
Ran for 87050 frames Cell Events: 5103 mean_circ: 0.947742
Ran for 87550 frames Cell Events: 5125 mean_circ: 0.947794
Ran for 89550 frames Cell Events: 5234 mean_circ: 0.947783
Ran for 90050 frames Cell Events: 5269 mean_circ: 0.947807
Ran for 91050 frames Cell Events: 5325 mean_circ: 0.947791
Ran for 91550 frames Cell Events: 5349 mean_circ: 0.947705
Ran for 94050 frames Cell Events: 5521 mean_circ: 0.947703
Ran for 94550 frames Cell Events: 5548 mean_circ: 0.947721
Ran for 95050 frames Cell Events: 5570 mean_circ: 0.947732
Ran for 96050 frames Cell Events: 5626 mean_circ: 0.947659
Ran for 100550 frames Cell Events: 5925 mean_circ: 0.947564
Ran for 101050 frames Cell Events: 5949 mean_circ: 0.947569
Ran for 101550 frames Cell Events: 5980 mean_circ: 0.947551
Ran for 102050 frames Cell Events: 6005 mean_circ: 0.947567
Ran for 102550 frames Cell Events: 6034 mean_circ: 0.947595
Ran for 105050 frames Cell Events: 6187 mean_circ: 0.947506
Ran for 105550 frames Cell Events: 6210 mean_circ: 0.947477
Ran for 106550 frames Cell Events: 6268 mean_circ: 0.947509
Ran for 107050 frames Cell Events: 6302 mean_circ: 0.947505
Ran for 108550 frames Cell Events: 6412 mean_circ: 0.947444
Ran for 109550 frames Cell Events: 6458 mean_circ: 0.947502
Ran for 111050 frames Cell Events: 6548 mean_circ: 0.947465
Ran for 112050 frames Cell Events: 6604 mean_circ: 0.947463
Ran for 112550 frames Cell Events: 6625 mean_circ: 0.947479
Ran for 113550 frames Cell Events: 6668 mean_circ: 0.947518
Ran for 114050 frames Cell Events: 6698 mean_circ: 0.947523
Ran for 115050 frames Cell Events: 6746 mean_circ: 0.94749
Ran for 117050 frames Cell Events: 6857 mean_circ: 0.947534
Ran for 120050 frames Cell Events: 6982 mean_circ: 0.947599
Ran for 120550 frames Cell Events: 6998 mean_circ: 0.947621
Ran for 123050 frames Cell Events: 7126 mean_circ: 0.947722
Ran for 123550 frames Cell Events: 7147 mean_circ: 0.947665
Ran for 124550 frames Cell Events: 7207 mean_circ: 0.9477
Ran for 126550 frames Cell Events: 7356 mean_circ: 0.947589
Ran for 127050 frames Cell Events: 7382 mean_circ: 0.947584
Ran for 127550 frames Cell Events: 7418 mean_circ: 0.947626
Ran for 128050 frames Cell Events: 7440 mean_circ: 0.947643
Ran for 129550 frames Cell Events: 7523 mean_circ: 0.947694
Ran for 130050 frames Cell Events: 7551 mean_circ: 0.947695
Ran for 131050 frames Cell Events: 7618 mean_circ: 0.947604
Ran for 133050 frames Cell Events: 7763 mean_circ: 0.947591
Ran for 133550 frames Cell Events: 7802 mean_circ: 0.947575
Ran for 134550 frames Cell Events: 7859 mean_circ: 0.947506
Ran for 135050 frames Cell Events: 7880 mean_circ: 0.947494
Ran for 135550 frames Cell Events: 7919 mean_circ: 0.947492
Ran for 137050 frames Cell Events: 8027 mean_circ: 0.947363
Ran for 138050 frames Cell Events: 8092 mean_circ: 0.947406
Ran for 139550 frames Cell Events: 8205 mean_circ: 0.947444



End this measurment..



Stopping time: 1.67199e+009
****************************************
Measurement finished

Mean Circularitiy: 0.947385
Mean Area: 627.485
Mean FL-1 intensity: 3628.01
Mean FL-2 intensity: 3442.02
Mean FL-3 intensity: 0

frames processed: 136206
Total Time: 69943.4ms
Number of Runs (new bg img) 0
Frames collected: 139749
Skipped frames: 66373
Rescued Frames: 2021165
Samples DAQ: 34938439
Samples written: 69874640
Samples per Channel: 34937320
FPS: 1998.03

Samples per second: 499508
Expected samples/s: 500000

Events: 8218
Events per second: 117.495
jumped back (PMT): 3013
#Frames read, frequency
# 0 frames :225354
# 1 frames :42437
# 2 frames :2565
# 3 frames :909
# 4 frames :569
# 5 frames :291
# 6 frames :1311
# 7 frames :1047
# 8 frames :860
# 9 frames :555
# 10 frames :485
# 11 frames :364
# 12 frames :285
# 13 frames :199
# 14 frames :241
# 15 frames :199
# 16 frames :197
# 17 frames :163
# 18 frames :124
# 19 frames :105
# 20 frames :93
# 21 frames :90
# 22 frames :75
# 23 frames :66
# 24 frames :65
# 25 frames :51
# 26 frames :34
# 27 frames :46
# 28 frames :32
# 29 frames :30
# 30 frames :27
# 31 frames :29
# 32 frames :24
# 33 frames :21
# 34 frames :20
# 35 frames :15
# 36 frames :18
# 37 frames :14
# 38 frames :15
# 39 frames :5
# 40 frames :7
# 41 frames :10
# 42 frames :11
# 43 frames :13
# 44 frames :4
# 45 frames :4
# 46 frames :5
# 47 frames :13
# 48 frames :6
# 49 frames :6
# 50 frames :6
# 51 frames :3
# 52 frames :3
# 53 frames :3
# 54 frames :3
# 55 frames :2
# 56 frames :2
# 57 frames :1
# 58 frames :2
# 59 frames :2
# 61 frames :1
# 62 frames :1
# 63 frames :1
# 64 frames :1
# 65 frames :2
# 68 frames :1
# 69 frames :3
# 70 frames :3
# 71 frames :1
# 74 frames :1
# 76 frames :1
# 85 frames :1
# 87 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.428
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0