Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_12.13.00 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "34e0953a9e0608451b4d2960c69e28fe",
"name": "M1_20170603_1650_dsRNA_M3_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "10:13:20"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8225 (max 8226) in '8225'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.432
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.67208e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 76 mean_circ: 0.933224
Ran for 2050 frames Cell Events: 171 mean_circ: 0.937143
Ran for 3550 frames Cell Events: 291 mean_circ: 0.936055
Ran for 5050 frames Cell Events: 400 mean_circ: 0.936407
Ran for 5550 frames Cell Events: 438 mean_circ: 0.936407
Ran for 7550 frames Cell Events: 581 mean_circ: 0.937272
Ran for 10050 frames Cell Events: 748 mean_circ: 0.937053
Ran for 10550 frames Cell Events: 781 mean_circ: 0.93723
Ran for 11050 frames Cell Events: 806 mean_circ: 0.937326
Ran for 11550 frames Cell Events: 828 mean_circ: 0.93715
Ran for 12050 frames Cell Events: 859 mean_circ: 0.93731
Ran for 12550 frames Cell Events: 886 mean_circ: 0.937632
Ran for 13050 frames Cell Events: 927 mean_circ: 0.937743
Ran for 16050 frames Cell Events: 1136 mean_circ: 0.938463
Ran for 18550 frames Cell Events: 1289 mean_circ: 0.938728
Ran for 19050 frames Cell Events: 1324 mean_circ: 0.938791
Ran for 19550 frames Cell Events: 1356 mean_circ: 0.93894
Ran for 20050 frames Cell Events: 1405 mean_circ: 0.93894
Ran for 20550 frames Cell Events: 1437 mean_circ: 0.93908
Ran for 21050 frames Cell Events: 1470 mean_circ: 0.93919
Ran for 22050 frames Cell Events: 1531 mean_circ: 0.939441
Ran for 22550 frames Cell Events: 1553 mean_circ: 0.939458
Ran for 23050 frames Cell Events: 1588 mean_circ: 0.93955
Ran for 24550 frames Cell Events: 1715 mean_circ: 0.93952
Ran for 25050 frames Cell Events: 1752 mean_circ: 0.939591
Ran for 26050 frames Cell Events: 1815 mean_circ: 0.939284
Ran for 26550 frames Cell Events: 1844 mean_circ: 0.939317
Ran for 27050 frames Cell Events: 1877 mean_circ: 0.938906
Ran for 28050 frames Cell Events: 1957 mean_circ: 0.938953
Ran for 29550 frames Cell Events: 2057 mean_circ: 0.938806
Ran for 30050 frames Cell Events: 2078 mean_circ: 0.938928
Ran for 30550 frames Cell Events: 2101 mean_circ: 0.938871
Ran for 33550 frames Cell Events: 2339 mean_circ: 0.938621
Ran for 35550 frames Cell Events: 2493 mean_circ: 0.938829
Ran for 37550 frames Cell Events: 2614 mean_circ: 0.938428
Ran for 38050 frames Cell Events: 2652 mean_circ: 0.938273
Ran for 43050 frames Cell Events: 2930 mean_circ: 0.937879
Ran for 43550 frames Cell Events: 2963 mean_circ: 0.937851
Ran for 44050 frames Cell Events: 2996 mean_circ: 0.937749
Ran for 45050 frames Cell Events: 3061 mean_circ: 0.937733
Ran for 46550 frames Cell Events: 3166 mean_circ: 0.9378
Ran for 47550 frames Cell Events: 3229 mean_circ: 0.937852
Ran for 48050 frames Cell Events: 3268 mean_circ: 0.937829
Ran for 49550 frames Cell Events: 3348 mean_circ: 0.937831
Ran for 52050 frames Cell Events: 3525 mean_circ: 0.937625
Ran for 52550 frames Cell Events: 3567 mean_circ: 0.937547
Ran for 55050 frames Cell Events: 3747 mean_circ: 0.937361
Ran for 56050 frames Cell Events: 3831 mean_circ: 0.937393
Ran for 56550 frames Cell Events: 3872 mean_circ: 0.937504
Ran for 57050 frames Cell Events: 3899 mean_circ: 0.937446
Ran for 57550 frames Cell Events: 3931 mean_circ: 0.937307
Ran for 58050 frames Cell Events: 3961 mean_circ: 0.937346
Ran for 61050 frames Cell Events: 4152 mean_circ: 0.937391
Ran for 61550 frames Cell Events: 4185 mean_circ: 0.937391
Ran for 63050 frames Cell Events: 4303 mean_circ: 0.937278
Ran for 64050 frames Cell Events: 4383 mean_circ: 0.93723
Ran for 64550 frames Cell Events: 4428 mean_circ: 0.937175
Ran for 66050 frames Cell Events: 4529 mean_circ: 0.937135
Ran for 67550 frames Cell Events: 4611 mean_circ: 0.937186
Ran for 70550 frames Cell Events: 4845 mean_circ: 0.937299
Ran for 71050 frames Cell Events: 4891 mean_circ: 0.937299
Ran for 72550 frames Cell Events: 4996 mean_circ: 0.937431
Ran for 73050 frames Cell Events: 5035 mean_circ: 0.937431
Ran for 73550 frames Cell Events: 5071 mean_circ: 0.937352
Ran for 74050 frames Cell Events: 5105 mean_circ: 0.937352
Ran for 75550 frames Cell Events: 5220 mean_circ: 0.937317
Ran for 76550 frames Cell Events: 5300 mean_circ: 0.937306
Ran for 77050 frames Cell Events: 5341 mean_circ: 0.937281
Ran for 77550 frames Cell Events: 5381 mean_circ: 0.937384
Ran for 81050 frames Cell Events: 5637 mean_circ: 0.937366
Ran for 81550 frames Cell Events: 5663 mean_circ: 0.937396
Ran for 82550 frames Cell Events: 5716 mean_circ: 0.937395
Ran for 84550 frames Cell Events: 5884 mean_circ: 0.937299
Ran for 86550 frames Cell Events: 6003 mean_circ: 0.937405
Ran for 87050 frames Cell Events: 6029 mean_circ: 0.937401
Ran for 87550 frames Cell Events: 6049 mean_circ: 0.937397
Ran for 88550 frames Cell Events: 6107 mean_circ: 0.937432
Ran for 90050 frames Cell Events: 6234 mean_circ: 0.937395
Ran for 91050 frames Cell Events: 6299 mean_circ: 0.937348
Ran for 91550 frames Cell Events: 6335 mean_circ: 0.937353
Ran for 92550 frames Cell Events: 6405 mean_circ: 0.937268
Ran for 94050 frames Cell Events: 6491 mean_circ: 0.937314
Ran for 95050 frames Cell Events: 6564 mean_circ: 0.937325
Ran for 97550 frames Cell Events: 6798 mean_circ: 0.937178
Ran for 98050 frames Cell Events: 6832 mean_circ: 0.937159
Ran for 99050 frames Cell Events: 6916 mean_circ: 0.937165
Ran for 99550 frames Cell Events: 6948 mean_circ: 0.937137
Ran for 100550 frames Cell Events: 7028 mean_circ: 0.937076
Ran for 101050 frames Cell Events: 7073 mean_circ: 0.937076
Ran for 101550 frames Cell Events: 7093 mean_circ: 0.937021
Ran for 103050 frames Cell Events: 7223 mean_circ: 0.93691
Ran for 103550 frames Cell Events: 7261 mean_circ: 0.936916
Ran for 104050 frames Cell Events: 7293 mean_circ: 0.936925
Ran for 106550 frames Cell Events: 7495 mean_circ: 0.936909
Ran for 107050 frames Cell Events: 7541 mean_circ: 0.936921
Ran for 111550 frames Cell Events: 7888 mean_circ: 0.937029
Ran for 112050 frames Cell Events: 7919 mean_circ: 0.936944
Ran for 112550 frames Cell Events: 7954 mean_circ: 0.936931



End this measurment..



Stopping time: 1.67213e+009
****************************************
Measurement finished

Mean Circularitiy: 0.936849
Mean Area: 622.805
Mean FL-1 intensity: 3392.86
Mean FL-2 intensity: 3629.67
Mean FL-3 intensity: 0

frames processed: 113163
Total Time: 58040.4ms
Number of Runs (new bg img) 0
Frames collected: 115947
Skipped frames: 69157
Rescued Frames: 2094127
Samples DAQ: 28990444
Samples written: 57973372
Samples per Channel: 28986686
FPS: 1997.69

Samples per second: 499422
Expected samples/s: 500000

Events: 8226
Events per second: 141.729
jumped back (PMT): 20
#Frames read, frequency
# 0 frames :186461
# 1 frames :30505
# 2 frames :1798
# 3 frames :791
# 4 frames :388
# 5 frames :223
# 6 frames :1634
# 7 frames :808
# 8 frames :714
# 9 frames :499
# 10 frames :433
# 11 frames :297
# 12 frames :228
# 13 frames :188
# 14 frames :220
# 15 frames :181
# 16 frames :183
# 17 frames :158
# 18 frames :111
# 19 frames :100
# 20 frames :95
# 21 frames :70
# 22 frames :73
# 23 frames :62
# 24 frames :51
# 25 frames :43
# 26 frames :40
# 27 frames :36
# 28 frames :34
# 29 frames :18
# 30 frames :22
# 31 frames :19
# 32 frames :15
# 33 frames :19
# 34 frames :22
# 35 frames :13
# 36 frames :13
# 37 frames :11
# 38 frames :9
# 39 frames :12
# 40 frames :11
# 41 frames :10
# 42 frames :7
# 43 frames :8
# 44 frames :4
# 45 frames :4
# 46 frames :6
# 47 frames :6
# 48 frames :2
# 49 frames :4
# 50 frames :3
# 51 frames :3
# 52 frames :3
# 53 frames :2
# 55 frames :2
# 56 frames :1
# 57 frames :1
# 59 frames :3
# 60 frames :1
# 61 frames :2
# 62 frames :1
# 63 frames :1
# 65 frames :2
# 66 frames :1
# 72 frames :1
# 73 frames :2
# 74 frames :2
# 75 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.432
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0