Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_12.13.27 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "702f24fed1e0767acbf5ec3f5b01c9e1",
"name": "M1_20170603_1658_dsRNA_C6_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "10:13:48"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8333 (max 8334) in '8333'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.433
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.67255e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 27 mean_circ: 0.936849
Ran for 4550 frames Cell Events: 313 mean_circ: 0.94422
Ran for 5050 frames Cell Events: 345 mean_circ: 0.944581
Ran for 7550 frames Cell Events: 546 mean_circ: 0.9445
Ran for 14050 frames Cell Events: 1002 mean_circ: 0.947164
Ran for 15050 frames Cell Events: 1074 mean_circ: 0.947128
Ran for 15550 frames Cell Events: 1108 mean_circ: 0.947056
Ran for 16550 frames Cell Events: 1170 mean_circ: 0.946847
Ran for 21050 frames Cell Events: 1486 mean_circ: 0.947409
Ran for 22050 frames Cell Events: 1542 mean_circ: 0.947617
Ran for 23050 frames Cell Events: 1604 mean_circ: 0.947887
Ran for 23550 frames Cell Events: 1628 mean_circ: 0.947876
Ran for 25050 frames Cell Events: 1728 mean_circ: 0.947858
Ran for 26050 frames Cell Events: 1809 mean_circ: 0.948193
Ran for 28550 frames Cell Events: 2007 mean_circ: 0.948242
Ran for 29050 frames Cell Events: 2029 mean_circ: 0.948324
Ran for 31050 frames Cell Events: 2168 mean_circ: 0.948402
Ran for 32550 frames Cell Events: 2269 mean_circ: 0.948333
Ran for 33550 frames Cell Events: 2339 mean_circ: 0.948641
Ran for 35050 frames Cell Events: 2441 mean_circ: 0.948788
Ran for 35550 frames Cell Events: 2477 mean_circ: 0.948725
Ran for 39050 frames Cell Events: 2729 mean_circ: 0.948629
Ran for 40550 frames Cell Events: 2820 mean_circ: 0.948769
Ran for 41550 frames Cell Events: 2885 mean_circ: 0.948947
Ran for 43550 frames Cell Events: 3042 mean_circ: 0.948835
Ran for 45050 frames Cell Events: 3126 mean_circ: 0.948771
Ran for 45550 frames Cell Events: 3149 mean_circ: 0.948739
Ran for 47050 frames Cell Events: 3231 mean_circ: 0.948644
Ran for 47550 frames Cell Events: 3272 mean_circ: 0.948724
Ran for 52550 frames Cell Events: 3606 mean_circ: 0.949131
Ran for 53050 frames Cell Events: 3628 mean_circ: 0.949018
Ran for 54550 frames Cell Events: 3742 mean_circ: 0.949166
Ran for 56050 frames Cell Events: 3836 mean_circ: 0.949142
Ran for 57050 frames Cell Events: 3880 mean_circ: 0.949187
Ran for 57550 frames Cell Events: 3905 mean_circ: 0.949211
Ran for 58050 frames Cell Events: 3939 mean_circ: 0.949279
Ran for 60050 frames Cell Events: 4089 mean_circ: 0.949215
Ran for 60550 frames Cell Events: 4115 mean_circ: 0.949267
Ran for 61550 frames Cell Events: 4172 mean_circ: 0.949286
Ran for 64050 frames Cell Events: 4310 mean_circ: 0.949209
Ran for 65550 frames Cell Events: 4438 mean_circ: 0.949198
Ran for 66550 frames Cell Events: 4505 mean_circ: 0.949238
Ran for 68550 frames Cell Events: 4645 mean_circ: 0.949153
Ran for 70050 frames Cell Events: 4750 mean_circ: 0.949169
Ran for 71050 frames Cell Events: 4770 mean_circ: 0.949169
Ran for 72550 frames Cell Events: 4882 mean_circ: 0.949254
Ran for 73050 frames Cell Events: 4920 mean_circ: 0.949309
Ran for 73550 frames Cell Events: 4948 mean_circ: 0.949308
Ran for 76050 frames Cell Events: 5099 mean_circ: 0.949339
Ran for 77550 frames Cell Events: 5195 mean_circ: 0.949415
Ran for 78550 frames Cell Events: 5278 mean_circ: 0.949415
Ran for 83050 frames Cell Events: 5544 mean_circ: 0.94958
Ran for 83550 frames Cell Events: 5586 mean_circ: 0.949563
Ran for 84550 frames Cell Events: 5669 mean_circ: 0.949571
Ran for 88050 frames Cell Events: 5927 mean_circ: 0.94969
Ran for 89050 frames Cell Events: 5999 mean_circ: 0.9496
Ran for 90050 frames Cell Events: 6072 mean_circ: 0.949572
Ran for 90550 frames Cell Events: 6106 mean_circ: 0.949585
Ran for 91550 frames Cell Events: 6192 mean_circ: 0.949545
Ran for 92550 frames Cell Events: 6251 mean_circ: 0.949489
Ran for 95050 frames Cell Events: 6436 mean_circ: 0.949611
Ran for 96050 frames Cell Events: 6491 mean_circ: 0.949673
Ran for 97050 frames Cell Events: 6566 mean_circ: 0.949687
Ran for 98550 frames Cell Events: 6670 mean_circ: 0.949677
Ran for 99050 frames Cell Events: 6705 mean_circ: 0.949735
Ran for 100550 frames Cell Events: 6829 mean_circ: 0.949763
Ran for 101550 frames Cell Events: 6912 mean_circ: 0.949771
Ran for 102550 frames Cell Events: 6984 mean_circ: 0.949804
Ran for 105550 frames Cell Events: 7184 mean_circ: 0.949828
Ran for 106050 frames Cell Events: 7216 mean_circ: 0.949883
Ran for 110050 frames Cell Events: 7526 mean_circ: 0.949839
Ran for 112550 frames Cell Events: 7719 mean_circ: 0.949707
Ran for 116050 frames Cell Events: 7993 mean_circ: 0.949813
Ran for 117050 frames Cell Events: 8065 mean_circ: 0.949813



End this measurment..



Stopping time: 1.67261e+009
****************************************
Measurement finished

Mean Circularitiy: 0.949729
Mean Area: 626.036
Mean FL-1 intensity: 3651.57
Mean FL-2 intensity: 3220.96
Mean FL-3 intensity: 0

frames processed: 114892
Total Time: 60309.3ms
Number of Runs (new bg img) 0
Frames collected: 120475
Skipped frames: 74740
Rescued Frames: 2173591
Samples DAQ: 30120575
Samples written: 60237566
Samples per Channel: 30118783
FPS: 1997.62

Samples per second: 499405
Expected samples/s: 500000

Events: 8334
Events per second: 138.188
jumped back (PMT): 2336
#Frames read, frequency
# 0 frames :191978
# 1 frames :25988
# 2 frames :1894
# 3 frames :686
# 4 frames :514
# 5 frames :285
# 6 frames :840
# 7 frames :828
# 8 frames :631
# 9 frames :515
# 10 frames :500
# 11 frames :349
# 12 frames :245
# 13 frames :171
# 14 frames :186
# 15 frames :166
# 16 frames :174
# 17 frames :160
# 18 frames :125
# 19 frames :102
# 20 frames :99
# 21 frames :115
# 22 frames :92
# 23 frames :80
# 24 frames :63
# 25 frames :62
# 26 frames :51
# 27 frames :37
# 28 frames :46
# 29 frames :34
# 30 frames :43
# 31 frames :35
# 32 frames :40
# 33 frames :28
# 34 frames :23
# 35 frames :20
# 36 frames :26
# 37 frames :28
# 38 frames :15
# 39 frames :10
# 40 frames :16
# 41 frames :18
# 42 frames :12
# 43 frames :15
# 44 frames :17
# 45 frames :10
# 46 frames :7
# 47 frames :7
# 48 frames :7
# 49 frames :7
# 50 frames :5
# 51 frames :7
# 52 frames :9
# 53 frames :4
# 54 frames :4
# 55 frames :6
# 56 frames :5
# 57 frames :4
# 58 frames :2
# 59 frames :3
# 60 frames :3
# 61 frames :2
# 62 frames :4
# 63 frames :1
# 64 frames :2
# 65 frames :2
# 66 frames :3
# 67 frames :2
# 68 frames :1
# 69 frames :1
# 70 frames :1
# 71 frames :1
# 73 frames :1
# 74 frames :2
# 75 frames :2
# 76 frames :1
# 83 frames :1
# 85 frames :1
# 93 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.433
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0