Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_12.15.27 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "38bc3f257d76f3dbf07db5ea8cf1441b",
"name": "M1_20170603_1723_dsRNA_B2_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "10:15:47"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8131 (max 8132) in '8131'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.4251
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.674e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 20 mean_circ: 0.94875
Ran for 1050 frames Cell Events: 45 mean_circ: 0.942552
Ran for 2550 frames Cell Events: 125 mean_circ: 0.948491
Ran for 3550 frames Cell Events: 162 mean_circ: 0.94763
Ran for 4550 frames Cell Events: 207 mean_circ: 0.946838
Ran for 6050 frames Cell Events: 263 mean_circ: 0.944195
Ran for 7050 frames Cell Events: 308 mean_circ: 0.943454
Ran for 8550 frames Cell Events: 397 mean_circ: 0.942576
Ran for 9550 frames Cell Events: 458 mean_circ: 0.942086
Ran for 11050 frames Cell Events: 522 mean_circ: 0.942239
Ran for 13050 frames Cell Events: 635 mean_circ: 0.941334
Ran for 15550 frames Cell Events: 780 mean_circ: 0.940901
Ran for 16050 frames Cell Events: 804 mean_circ: 0.941232
Ran for 18050 frames Cell Events: 905 mean_circ: 0.941472
Ran for 18550 frames Cell Events: 928 mean_circ: 0.941105
Ran for 20050 frames Cell Events: 1010 mean_circ: 0.940982
Ran for 21050 frames Cell Events: 1053 mean_circ: 0.941502
Ran for 22550 frames Cell Events: 1138 mean_circ: 0.941368
Ran for 23550 frames Cell Events: 1192 mean_circ: 0.94143
Ran for 26050 frames Cell Events: 1323 mean_circ: 0.94012
Ran for 26550 frames Cell Events: 1352 mean_circ: 0.94018
Ran for 27550 frames Cell Events: 1414 mean_circ: 0.940326
Ran for 28050 frames Cell Events: 1434 mean_circ: 0.940489
Ran for 30550 frames Cell Events: 1549 mean_circ: 0.940763
Ran for 31550 frames Cell Events: 1608 mean_circ: 0.940915
Ran for 32050 frames Cell Events: 1632 mean_circ: 0.940765
Ran for 33050 frames Cell Events: 1660 mean_circ: 0.940577
Ran for 34050 frames Cell Events: 1725 mean_circ: 0.940488
Ran for 34550 frames Cell Events: 1747 mean_circ: 0.940485
Ran for 37050 frames Cell Events: 1899 mean_circ: 0.940662
Ran for 40050 frames Cell Events: 2092 mean_circ: 0.940656
Ran for 41050 frames Cell Events: 2146 mean_circ: 0.940523
Ran for 41550 frames Cell Events: 2163 mean_circ: 0.940596
Ran for 42550 frames Cell Events: 2215 mean_circ: 0.940374
Ran for 44050 frames Cell Events: 2291 mean_circ: 0.940416
Ran for 44550 frames Cell Events: 2302 mean_circ: 0.940461
Ran for 45050 frames Cell Events: 2323 mean_circ: 0.940588
Ran for 47550 frames Cell Events: 2471 mean_circ: 0.940524
Ran for 48050 frames Cell Events: 2484 mean_circ: 0.940567
Ran for 51050 frames Cell Events: 2658 mean_circ: 0.940087
Ran for 51550 frames Cell Events: 2690 mean_circ: 0.940167
Ran for 53050 frames Cell Events: 2774 mean_circ: 0.940105
Ran for 53550 frames Cell Events: 2801 mean_circ: 0.940188
Ran for 55050 frames Cell Events: 2926 mean_circ: 0.940461
Ran for 56550 frames Cell Events: 3035 mean_circ: 0.940513
Ran for 59050 frames Cell Events: 3166 mean_circ: 0.940465
Ran for 59550 frames Cell Events: 3191 mean_circ: 0.940302
Ran for 60550 frames Cell Events: 3255 mean_circ: 0.940223
Ran for 61550 frames Cell Events: 3318 mean_circ: 0.940195
Ran for 63550 frames Cell Events: 3445 mean_circ: 0.94027
Ran for 64550 frames Cell Events: 3503 mean_circ: 0.94014
Ran for 68550 frames Cell Events: 3763 mean_circ: 0.940013
Ran for 69050 frames Cell Events: 3789 mean_circ: 0.939976
Ran for 69550 frames Cell Events: 3820 mean_circ: 0.939949
Ran for 72050 frames Cell Events: 3970 mean_circ: 0.940064
Ran for 73050 frames Cell Events: 4026 mean_circ: 0.940123
Ran for 75550 frames Cell Events: 4164 mean_circ: 0.940162
Ran for 76550 frames Cell Events: 4209 mean_circ: 0.940081
Ran for 78050 frames Cell Events: 4302 mean_circ: 0.940113
Ran for 80550 frames Cell Events: 4462 mean_circ: 0.940074
Ran for 81050 frames Cell Events: 4480 mean_circ: 0.940118
Ran for 82050 frames Cell Events: 4530 mean_circ: 0.940156
Ran for 82550 frames Cell Events: 4555 mean_circ: 0.940155
Ran for 84050 frames Cell Events: 4656 mean_circ: 0.940123
Ran for 86550 frames Cell Events: 4795 mean_circ: 0.940166
Ran for 88050 frames Cell Events: 4881 mean_circ: 0.940232
Ran for 88550 frames Cell Events: 4894 mean_circ: 0.940266
Ran for 89550 frames Cell Events: 4946 mean_circ: 0.940233
Ran for 91050 frames Cell Events: 5043 mean_circ: 0.940247
Ran for 92050 frames Cell Events: 5101 mean_circ: 0.940282
Ran for 92550 frames Cell Events: 5124 mean_circ: 0.940301
Ran for 95550 frames Cell Events: 5292 mean_circ: 0.940364
Ran for 96050 frames Cell Events: 5316 mean_circ: 0.940361
Ran for 98550 frames Cell Events: 5452 mean_circ: 0.940514
Ran for 100550 frames Cell Events: 5568 mean_circ: 0.9405
Ran for 102050 frames Cell Events: 5653 mean_circ: 0.940566
Ran for 102550 frames Cell Events: 5672 mean_circ: 0.940498
Ran for 103050 frames Cell Events: 5702 mean_circ: 0.940498
Ran for 104050 frames Cell Events: 5750 mean_circ: 0.940505
Ran for 105050 frames Cell Events: 5808 mean_circ: 0.940533
Ran for 106050 frames Cell Events: 5860 mean_circ: 0.940568
Ran for 106550 frames Cell Events: 5881 mean_circ: 0.940494
Ran for 107050 frames Cell Events: 5903 mean_circ: 0.940483
Ran for 108050 frames Cell Events: 5954 mean_circ: 0.940452
Ran for 109050 frames Cell Events: 5982 mean_circ: 0.940444
Ran for 113050 frames Cell Events: 6206 mean_circ: 0.940405
Ran for 113550 frames Cell Events: 6230 mean_circ: 0.940371
Ran for 114550 frames Cell Events: 6305 mean_circ: 0.940247
Ran for 117550 frames Cell Events: 6463 mean_circ: 0.940269
Ran for 118050 frames Cell Events: 6493 mean_circ: 0.940283
Ran for 118550 frames Cell Events: 6516 mean_circ: 0.940304
Ran for 119050 frames Cell Events: 6540 mean_circ: 0.940251
Ran for 120050 frames Cell Events: 6598 mean_circ: 0.940363
Ran for 124050 frames Cell Events: 6821 mean_circ: 0.940351
Ran for 125050 frames Cell Events: 6878 mean_circ: 0.940362
Ran for 126050 frames Cell Events: 6931 mean_circ: 0.940289
Ran for 126550 frames Cell Events: 6962 mean_circ: 0.94028
Ran for 128550 frames Cell Events: 7087 mean_circ: 0.940212
Ran for 129550 frames Cell Events: 7155 mean_circ: 0.940289
Ran for 130550 frames Cell Events: 7200 mean_circ: 0.940339
Ran for 135050 frames Cell Events: 7483 mean_circ: 0.940481
Ran for 135550 frames Cell Events: 7513 mean_circ: 0.940481
Ran for 137550 frames Cell Events: 7630 mean_circ: 0.940482
Ran for 139050 frames Cell Events: 7732 mean_circ: 0.94045
Ran for 141050 frames Cell Events: 7847 mean_circ: 0.94044
Ran for 142550 frames Cell Events: 7931 mean_circ: 0.940429
Ran for 143050 frames Cell Events: 7957 mean_circ: 0.940344
Ran for 143550 frames Cell Events: 7979 mean_circ: 0.940322



End this measurment..



Stopping time: 1.67408e+009
****************************************
Measurement finished

Mean Circularitiy: 0.940328
Mean Area: 628.129
Mean FL-1 intensity: 2832.81
Mean FL-2 intensity: 2925.15
Mean FL-3 intensity: 0

frames processed: 140513
Total Time: 72725.1ms
Number of Runs (new bg img) 0
Frames collected: 145309
Skipped frames: 92358
Rescued Frames: 2525200
Samples DAQ: 36327753
Samples written: 72654176
Samples per Channel: 36327088
FPS: 1998.06

Samples per second: 499513
Expected samples/s: 500000

Events: 8132
Events per second: 111.818
jumped back (PMT): 2520
#Frames read, frequency
# 0 frames :237211
# 1 frames :39335
# 2 frames :2180
# 3 frames :917
# 4 frames :569
# 5 frames :360
# 6 frames :793
# 7 frames :870
# 8 frames :727
# 9 frames :481
# 10 frames :571
# 11 frames :386
# 12 frames :315
# 13 frames :229
# 14 frames :225
# 15 frames :218
# 16 frames :199
# 17 frames :181
# 18 frames :142
# 19 frames :127
# 20 frames :130
# 21 frames :112
# 22 frames :101
# 23 frames :81
# 24 frames :88
# 25 frames :83
# 26 frames :65
# 27 frames :57
# 28 frames :63
# 29 frames :50
# 30 frames :46
# 31 frames :35
# 32 frames :35
# 33 frames :31
# 34 frames :29
# 35 frames :28
# 36 frames :22
# 37 frames :22
# 38 frames :13
# 39 frames :25
# 40 frames :17
# 41 frames :13
# 42 frames :15
# 43 frames :7
# 44 frames :13
# 45 frames :8
# 46 frames :9
# 47 frames :13
# 48 frames :12
# 49 frames :8
# 50 frames :5
# 51 frames :10
# 52 frames :4
# 53 frames :4
# 54 frames :6
# 55 frames :5
# 56 frames :3
# 57 frames :2
# 58 frames :3
# 59 frames :1
# 60 frames :2
# 61 frames :1
# 62 frames :2
# 63 frames :4
# 64 frames :1
# 65 frames :5
# 66 frames :1
# 67 frames :2
# 68 frames :3
# 69 frames :1
# 70 frames :2
# 71 frames :1
# 72 frames :2
# 75 frames :1
# 76 frames :2
# 79 frames :1
# 82 frames :1
# 84 frames :1
# 87 frames :1
# 95 frames :1
# 96 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.4251
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0