Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_12.15.54 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "360f8f02e1331a1270056f62a76f69ed",
"name": "M1_20170603_1725_dsRNA_B2_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "10:16:14"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8062 (max 8064) in '8062'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.427
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.67417e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 36 mean_circ: 0.871244
Ran for 1050 frames Cell Events: 63 mean_circ: 0.906316
Ran for 2050 frames Cell Events: 130 mean_circ: 0.924436
Ran for 2550 frames Cell Events: 176 mean_circ: 0.925401
Ran for 4050 frames Cell Events: 293 mean_circ: 0.922441
Ran for 6550 frames Cell Events: 458 mean_circ: 0.926658
Ran for 7050 frames Cell Events: 492 mean_circ: 0.927993
Ran for 7550 frames Cell Events: 520 mean_circ: 0.928009
Ran for 8050 frames Cell Events: 548 mean_circ: 0.928635
Ran for 8550 frames Cell Events: 579 mean_circ: 0.928492
Ran for 9050 frames Cell Events: 611 mean_circ: 0.928925
Ran for 10050 frames Cell Events: 673 mean_circ: 0.929045
Ran for 11550 frames Cell Events: 776 mean_circ: 0.928994
Ran for 12050 frames Cell Events: 810 mean_circ: 0.928245
Ran for 12550 frames Cell Events: 846 mean_circ: 0.928323
Ran for 13550 frames Cell Events: 897 mean_circ: 0.928041
Ran for 15050 frames Cell Events: 1005 mean_circ: 0.928928
Ran for 15550 frames Cell Events: 1024 mean_circ: 0.928929
Ran for 16050 frames Cell Events: 1057 mean_circ: 0.928953
Ran for 17050 frames Cell Events: 1114 mean_circ: 0.92957
Ran for 17550 frames Cell Events: 1143 mean_circ: 0.929476
Ran for 18050 frames Cell Events: 1176 mean_circ: 0.929425
Ran for 18550 frames Cell Events: 1214 mean_circ: 0.929425
Ran for 19050 frames Cell Events: 1248 mean_circ: 0.92918
Ran for 19550 frames Cell Events: 1273 mean_circ: 0.929042
Ran for 20050 frames Cell Events: 1312 mean_circ: 0.928638
Ran for 21050 frames Cell Events: 1374 mean_circ: 0.928729
Ran for 22050 frames Cell Events: 1448 mean_circ: 0.929198
Ran for 23550 frames Cell Events: 1532 mean_circ: 0.929182
Ran for 24550 frames Cell Events: 1597 mean_circ: 0.928985
Ran for 25550 frames Cell Events: 1662 mean_circ: 0.929152
Ran for 27050 frames Cell Events: 1766 mean_circ: 0.929074
Ran for 27550 frames Cell Events: 1803 mean_circ: 0.929211
Ran for 28050 frames Cell Events: 1835 mean_circ: 0.929258
Ran for 30050 frames Cell Events: 1969 mean_circ: 0.929267
Ran for 30550 frames Cell Events: 2002 mean_circ: 0.929159
Ran for 32550 frames Cell Events: 2147 mean_circ: 0.928981
Ran for 33050 frames Cell Events: 2169 mean_circ: 0.929091
Ran for 33550 frames Cell Events: 2201 mean_circ: 0.929027
Ran for 35050 frames Cell Events: 2311 mean_circ: 0.929176
Ran for 36050 frames Cell Events: 2358 mean_circ: 0.929025
Ran for 36550 frames Cell Events: 2379 mean_circ: 0.929268
Ran for 38050 frames Cell Events: 2494 mean_circ: 0.929168
Ran for 41550 frames Cell Events: 2715 mean_circ: 0.92919
Ran for 42050 frames Cell Events: 2742 mean_circ: 0.929406
Ran for 47050 frames Cell Events: 3050 mean_circ: 0.929866
Ran for 48050 frames Cell Events: 3119 mean_circ: 0.929993
Ran for 49050 frames Cell Events: 3175 mean_circ: 0.929878
Ran for 49550 frames Cell Events: 3196 mean_circ: 0.929747
Ran for 50050 frames Cell Events: 3221 mean_circ: 0.929754
Ran for 50550 frames Cell Events: 3252 mean_circ: 0.929775
Ran for 51050 frames Cell Events: 3289 mean_circ: 0.929936
Ran for 51550 frames Cell Events: 3320 mean_circ: 0.929951
Ran for 52050 frames Cell Events: 3362 mean_circ: 0.930142
Ran for 53550 frames Cell Events: 3462 mean_circ: 0.930232
Ran for 54550 frames Cell Events: 3527 mean_circ: 0.930049
Ran for 55050 frames Cell Events: 3561 mean_circ: 0.930039
Ran for 57050 frames Cell Events: 3716 mean_circ: 0.930025
Ran for 57550 frames Cell Events: 3744 mean_circ: 0.930075
Ran for 58050 frames Cell Events: 3786 mean_circ: 0.930075
Ran for 59050 frames Cell Events: 3838 mean_circ: 0.929839
Ran for 60550 frames Cell Events: 3916 mean_circ: 0.929696
Ran for 61550 frames Cell Events: 3982 mean_circ: 0.929733
Ran for 62050 frames Cell Events: 4008 mean_circ: 0.929633
Ran for 63550 frames Cell Events: 4103 mean_circ: 0.929579
Ran for 65050 frames Cell Events: 4192 mean_circ: 0.929617
Ran for 66550 frames Cell Events: 4271 mean_circ: 0.929587
Ran for 67050 frames Cell Events: 4294 mean_circ: 0.929598
Ran for 69550 frames Cell Events: 4421 mean_circ: 0.929815
Ran for 71050 frames Cell Events: 4521 mean_circ: 0.929774
Ran for 71550 frames Cell Events: 4553 mean_circ: 0.929761
Ran for 72050 frames Cell Events: 4595 mean_circ: 0.929761
Ran for 75550 frames Cell Events: 4814 mean_circ: 0.929388
Ran for 78050 frames Cell Events: 4963 mean_circ: 0.929427
Ran for 78550 frames Cell Events: 4987 mean_circ: 0.929494
Ran for 79550 frames Cell Events: 5053 mean_circ: 0.929367
Ran for 81550 frames Cell Events: 5135 mean_circ: 0.929283
Ran for 82050 frames Cell Events: 5162 mean_circ: 0.929209
Ran for 82550 frames Cell Events: 5186 mean_circ: 0.929192
Ran for 84050 frames Cell Events: 5300 mean_circ: 0.929113
Ran for 85050 frames Cell Events: 5365 mean_circ: 0.929102
Ran for 88050 frames Cell Events: 5543 mean_circ: 0.929074
Ran for 89550 frames Cell Events: 5639 mean_circ: 0.929051
Ran for 90050 frames Cell Events: 5666 mean_circ: 0.929107
Ran for 93550 frames Cell Events: 5892 mean_circ: 0.929036
Ran for 94050 frames Cell Events: 5918 mean_circ: 0.928967
Ran for 95550 frames Cell Events: 6024 mean_circ: 0.928814
Ran for 96050 frames Cell Events: 6060 mean_circ: 0.928841
Ran for 96550 frames Cell Events: 6095 mean_circ: 0.928849
Ran for 99050 frames Cell Events: 6266 mean_circ: 0.928912
Ran for 99550 frames Cell Events: 6307 mean_circ: 0.928939
Ran for 100050 frames Cell Events: 6337 mean_circ: 0.928965
Ran for 101550 frames Cell Events: 6432 mean_circ: 0.929051
Ran for 102050 frames Cell Events: 6455 mean_circ: 0.929008
Ran for 103050 frames Cell Events: 6524 mean_circ: 0.928942
Ran for 103550 frames Cell Events: 6549 mean_circ: 0.928957
Ran for 105050 frames Cell Events: 6653 mean_circ: 0.928834
Ran for 106050 frames Cell Events: 6718 mean_circ: 0.928873
Ran for 107050 frames Cell Events: 6790 mean_circ: 0.928933
Ran for 110550 frames Cell Events: 7030 mean_circ: 0.928776
Ran for 111550 frames Cell Events: 7086 mean_circ: 0.928804
Ran for 112050 frames Cell Events: 7128 mean_circ: 0.928797
Ran for 116550 frames Cell Events: 7429 mean_circ: 0.928705
Ran for 118050 frames Cell Events: 7527 mean_circ: 0.928688
Ran for 118550 frames Cell Events: 7572 mean_circ: 0.928662
Ran for 119050 frames Cell Events: 7603 mean_circ: 0.928687
Ran for 120050 frames Cell Events: 7667 mean_circ: 0.928526
Ran for 121050 frames Cell Events: 7736 mean_circ: 0.928508
Ran for 121550 frames Cell Events: 7754 mean_circ: 0.928425
Ran for 124050 frames Cell Events: 7904 mean_circ: 0.928464
Ran for 124550 frames Cell Events: 7928 mean_circ: 0.928462
Ran for 125550 frames Cell Events: 7984 mean_circ: 0.928477
Ran for 126050 frames Cell Events: 8006 mean_circ: 0.928489
Ran for 126550 frames Cell Events: 8046 mean_circ: 0.928464



End this measurment..



Stopping time: 1.67423e+009
****************************************
Measurement finished

Mean Circularitiy: 0.928525
Mean Area: 624.196
Mean FL-1 intensity: 2668.22
Mean FL-2 intensity: 3145.19
Mean FL-3 intensity: 0

frames processed: 123079
Total Time: 63534.2ms
Number of Runs (new bg img) 0
Frames collected: 126943
Skipped frames: 96222
Rescued Frames: 2599742
Samples DAQ: 31736294
Samples written: 63471282
Samples per Channel: 31735641
FPS: 1998.02

Samples per second: 499505
Expected samples/s: 500000

Events: 8064
Events per second: 126.924
jumped back (PMT): 20
#Frames read, frequency
# 0 frames :205289
# 1 frames :38273
# 2 frames :2147
# 3 frames :821
# 4 frames :462
# 5 frames :240
# 6 frames :1480
# 7 frames :935
# 8 frames :730
# 9 frames :489
# 10 frames :444
# 11 frames :370
# 12 frames :287
# 13 frames :168
# 14 frames :222
# 15 frames :186
# 16 frames :180
# 17 frames :163
# 18 frames :116
# 19 frames :88
# 20 frames :102
# 21 frames :84
# 22 frames :72
# 23 frames :48
# 24 frames :54
# 25 frames :38
# 26 frames :33
# 27 frames :42
# 28 frames :26
# 29 frames :20
# 30 frames :22
# 31 frames :22
# 32 frames :29
# 33 frames :14
# 34 frames :16
# 35 frames :18
# 36 frames :14
# 37 frames :10
# 38 frames :8
# 39 frames :6
# 40 frames :10
# 41 frames :6
# 42 frames :9
# 43 frames :11
# 44 frames :1
# 45 frames :4
# 46 frames :6
# 47 frames :1
# 48 frames :4
# 49 frames :4
# 50 frames :2
# 51 frames :3
# 52 frames :2
# 53 frames :1
# 54 frames :2
# 55 frames :3
# 56 frames :1
# 57 frames :4
# 58 frames :1
# 61 frames :1
# 63 frames :1
# 64 frames :2
# 65 frames :2
# 66 frames :2
# 67 frames :1
# 71 frames :1
# 77 frames :1
# 86 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.427
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0