Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_12.21.14 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "6b9ac70b6793fa3390ade6992d2d7386",
"name": "M1_20170603_1839_dsRNA_C8_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "10:21:35"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8202 (max 8203) in '8202'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.8094
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.6786e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 50 frames Cell Events: 3 mean_circ: 0.947573
Ran for 2550 frames Cell Events: 135 mean_circ: 0.937891
Ran for 3050 frames Cell Events: 159 mean_circ: 0.937831
Ran for 5550 frames Cell Events: 293 mean_circ: 0.938222
Ran for 7550 frames Cell Events: 402 mean_circ: 0.937962
Ran for 8550 frames Cell Events: 447 mean_circ: 0.939163
Ran for 9550 frames Cell Events: 497 mean_circ: 0.937523
Ran for 11050 frames Cell Events: 553 mean_circ: 0.938197
Ran for 17050 frames Cell Events: 953 mean_circ: 0.937562
Ran for 19050 frames Cell Events: 1083 mean_circ: 0.936593
Ran for 22550 frames Cell Events: 1260 mean_circ: 0.936755
Ran for 23050 frames Cell Events: 1293 mean_circ: 0.936589
Ran for 23550 frames Cell Events: 1312 mean_circ: 0.936476
Ran for 24550 frames Cell Events: 1372 mean_circ: 0.936504
Ran for 25050 frames Cell Events: 1400 mean_circ: 0.936267
Ran for 27550 frames Cell Events: 1569 mean_circ: 0.935489
Ran for 28050 frames Cell Events: 1607 mean_circ: 0.93559
Ran for 29550 frames Cell Events: 1692 mean_circ: 0.935892
Ran for 31050 frames Cell Events: 1807 mean_circ: 0.93604
Ran for 32050 frames Cell Events: 1875 mean_circ: 0.935727
Ran for 33050 frames Cell Events: 1925 mean_circ: 0.935712
Ran for 35550 frames Cell Events: 2089 mean_circ: 0.936188
Ran for 36050 frames Cell Events: 2114 mean_circ: 0.936078
Ran for 37050 frames Cell Events: 2182 mean_circ: 0.936239
Ran for 37550 frames Cell Events: 2216 mean_circ: 0.936169
Ran for 39550 frames Cell Events: 2314 mean_circ: 0.936227
Ran for 40050 frames Cell Events: 2344 mean_circ: 0.936236
Ran for 40550 frames Cell Events: 2368 mean_circ: 0.936324
Ran for 42050 frames Cell Events: 2461 mean_circ: 0.936386
Ran for 42550 frames Cell Events: 2489 mean_circ: 0.936415
Ran for 45550 frames Cell Events: 2644 mean_circ: 0.936086
Ran for 48550 frames Cell Events: 2789 mean_circ: 0.936327
Ran for 49050 frames Cell Events: 2808 mean_circ: 0.936327
Ran for 50050 frames Cell Events: 2844 mean_circ: 0.936337
Ran for 51050 frames Cell Events: 2913 mean_circ: 0.936337
Ran for 53050 frames Cell Events: 3015 mean_circ: 0.936352
Ran for 54050 frames Cell Events: 3078 mean_circ: 0.936287
Ran for 54550 frames Cell Events: 3097 mean_circ: 0.936256
Ran for 55550 frames Cell Events: 3132 mean_circ: 0.936122
Ran for 56550 frames Cell Events: 3190 mean_circ: 0.936187
Ran for 58550 frames Cell Events: 3278 mean_circ: 0.936035
Ran for 60050 frames Cell Events: 3360 mean_circ: 0.936012
Ran for 61050 frames Cell Events: 3431 mean_circ: 0.935942
Ran for 62050 frames Cell Events: 3506 mean_circ: 0.935875
Ran for 62550 frames Cell Events: 3527 mean_circ: 0.935711
Ran for 63050 frames Cell Events: 3558 mean_circ: 0.935687
Ran for 64550 frames Cell Events: 3669 mean_circ: 0.935567
Ran for 65050 frames Cell Events: 3701 mean_circ: 0.935582
Ran for 67050 frames Cell Events: 3840 mean_circ: 0.935551
Ran for 68050 frames Cell Events: 3882 mean_circ: 0.935583
Ran for 70050 frames Cell Events: 4035 mean_circ: 0.935806
Ran for 70550 frames Cell Events: 4069 mean_circ: 0.93579
Ran for 71050 frames Cell Events: 4093 mean_circ: 0.935715
Ran for 72050 frames Cell Events: 4153 mean_circ: 0.935797
Ran for 72550 frames Cell Events: 4177 mean_circ: 0.935839
Ran for 73050 frames Cell Events: 4205 mean_circ: 0.935782
Ran for 73550 frames Cell Events: 4231 mean_circ: 0.93579
Ran for 74550 frames Cell Events: 4304 mean_circ: 0.935749
Ran for 75550 frames Cell Events: 4363 mean_circ: 0.935757
Ran for 77550 frames Cell Events: 4479 mean_circ: 0.935724
Ran for 78050 frames Cell Events: 4514 mean_circ: 0.935735
Ran for 78550 frames Cell Events: 4543 mean_circ: 0.935831
Ran for 80550 frames Cell Events: 4648 mean_circ: 0.935881
Ran for 81050 frames Cell Events: 4669 mean_circ: 0.935831
Ran for 82050 frames Cell Events: 4730 mean_circ: 0.935975
Ran for 83550 frames Cell Events: 4819 mean_circ: 0.935917
Ran for 85050 frames Cell Events: 4866 mean_circ: 0.935918
Ran for 86550 frames Cell Events: 4938 mean_circ: 0.935874
Ran for 88050 frames Cell Events: 5022 mean_circ: 0.935917
Ran for 89050 frames Cell Events: 5090 mean_circ: 0.935796
Ran for 90050 frames Cell Events: 5155 mean_circ: 0.935753
Ran for 91050 frames Cell Events: 5234 mean_circ: 0.935706
Ran for 91550 frames Cell Events: 5275 mean_circ: 0.935746
Ran for 92050 frames Cell Events: 5304 mean_circ: 0.9357
Ran for 92550 frames Cell Events: 5330 mean_circ: 0.935678
Ran for 93050 frames Cell Events: 5351 mean_circ: 0.935666
Ran for 93550 frames Cell Events: 5386 mean_circ: 0.935675
Ran for 94050 frames Cell Events: 5415 mean_circ: 0.935656
Ran for 95550 frames Cell Events: 5521 mean_circ: 0.935673
Ran for 96050 frames Cell Events: 5543 mean_circ: 0.935687
Ran for 96550 frames Cell Events: 5555 mean_circ: 0.935711
Ran for 97050 frames Cell Events: 5585 mean_circ: 0.935678
Ran for 97550 frames Cell Events: 5622 mean_circ: 0.93564
Ran for 98050 frames Cell Events: 5659 mean_circ: 0.935533
Ran for 98550 frames Cell Events: 5694 mean_circ: 0.935554
Ran for 99050 frames Cell Events: 5736 mean_circ: 0.935624
Ran for 100050 frames Cell Events: 5814 mean_circ: 0.935587
Ran for 100550 frames Cell Events: 5846 mean_circ: 0.93555
Ran for 101550 frames Cell Events: 5929 mean_circ: 0.935392
Ran for 103050 frames Cell Events: 6014 mean_circ: 0.935355
Ran for 103550 frames Cell Events: 6037 mean_circ: 0.935356
Ran for 105050 frames Cell Events: 6124 mean_circ: 0.935334
Ran for 105550 frames Cell Events: 6151 mean_circ: 0.93539
Ran for 106050 frames Cell Events: 6170 mean_circ: 0.935388
Ran for 106550 frames Cell Events: 6205 mean_circ: 0.935437
Ran for 107050 frames Cell Events: 6238 mean_circ: 0.935455
Ran for 107550 frames Cell Events: 6272 mean_circ: 0.93551
Ran for 108050 frames Cell Events: 6304 mean_circ: 0.935491
Ran for 108550 frames Cell Events: 6349 mean_circ: 0.93551
Ran for 109050 frames Cell Events: 6368 mean_circ: 0.935501
Ran for 109550 frames Cell Events: 6391 mean_circ: 0.935476
Ran for 110050 frames Cell Events: 6425 mean_circ: 0.935423
Ran for 111050 frames Cell Events: 6503 mean_circ: 0.935364
Ran for 112550 frames Cell Events: 6592 mean_circ: 0.935354
Ran for 113050 frames Cell Events: 6619 mean_circ: 0.93538
Ran for 115550 frames Cell Events: 6780 mean_circ: 0.935464
Ran for 116050 frames Cell Events: 6790 mean_circ: 0.935466
Ran for 116550 frames Cell Events: 6813 mean_circ: 0.935446
Ran for 118550 frames Cell Events: 6978 mean_circ: 0.935429
Ran for 119550 frames Cell Events: 7047 mean_circ: 0.935468
Ran for 120050 frames Cell Events: 7076 mean_circ: 0.935424
Ran for 120550 frames Cell Events: 7099 mean_circ: 0.935439
Ran for 124050 frames Cell Events: 7316 mean_circ: 0.93542
Ran for 124550 frames Cell Events: 7339 mean_circ: 0.935445
Ran for 127550 frames Cell Events: 7509 mean_circ: 0.935431
Ran for 128050 frames Cell Events: 7528 mean_circ: 0.935469
Ran for 129050 frames Cell Events: 7589 mean_circ: 0.935469
Ran for 130050 frames Cell Events: 7627 mean_circ: 0.935472
Ran for 130550 frames Cell Events: 7647 mean_circ: 0.935464
Ran for 131050 frames Cell Events: 7674 mean_circ: 0.935476
Ran for 132050 frames Cell Events: 7731 mean_circ: 0.935484
Ran for 135050 frames Cell Events: 7932 mean_circ: 0.935575
Ran for 136050 frames Cell Events: 8001 mean_circ: 0.935582



End this measurment..



Stopping time: 1.67867e+009
****************************************
Measurement finished

Mean Circularitiy: 0.935339
Mean Area: 636.047
Mean FL-1 intensity: 3476.93
Mean FL-2 intensity: 4248.47
Mean FL-3 intensity: 0

frames processed: 133109
Total Time: 69476.7ms
Number of Runs (new bg img) 0
Frames collected: 138800
Skipped frames: 143249
Rescued Frames: 3791470
Samples DAQ: 34702002
Samples written: 69399740
Samples per Channel: 34699870
FPS: 1997.79

Samples per second: 499446
Expected samples/s: 500000

Events: 8203
Events per second: 118.068
jumped back (PMT): 32
#Frames read, frequency
# 0 frames :220704
# 1 frames :41828
# 2 frames :2361
# 3 frames :935
# 4 frames :511
# 5 frames :289
# 6 frames :1695
# 7 frames :955
# 8 frames :882
# 9 frames :606
# 10 frames :423
# 11 frames :322
# 12 frames :285
# 13 frames :202
# 14 frames :251
# 15 frames :185
# 16 frames :167
# 17 frames :125
# 18 frames :105
# 19 frames :91
# 20 frames :100
# 21 frames :84
# 22 frames :74
# 23 frames :59
# 24 frames :56
# 25 frames :41
# 26 frames :44
# 27 frames :42
# 28 frames :39
# 29 frames :36
# 30 frames :28
# 31 frames :27
# 32 frames :20
# 33 frames :22
# 34 frames :23
# 35 frames :13
# 36 frames :12
# 37 frames :18
# 38 frames :10
# 39 frames :10
# 40 frames :8
# 41 frames :7
# 42 frames :8
# 43 frames :7
# 44 frames :6
# 45 frames :4
# 46 frames :3
# 47 frames :5
# 48 frames :5
# 49 frames :4
# 50 frames :4
# 51 frames :8
# 52 frames :5
# 53 frames :5
# 54 frames :1
# 55 frames :1
# 57 frames :4
# 59 frames :7
# 61 frames :2
# 62 frames :1
# 63 frames :1
# 65 frames :1
# 67 frames :2
# 70 frames :1
# 73 frames :3
# 75 frames :1
# 76 frames :1
# 80 frames :1
# 94 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.8094
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0