Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_12.24.54 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "fab46a1b5bb62d137ef770ad0ae51c68",
"name": "M1_20170603_1927_dsRNA_D1_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "10:25:17"
}
}
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.0103
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.68147e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 50 frames Cell Events: 0 mean_circ: 0.943952
Ran for 550 frames Cell Events: 16 mean_circ: 0.933746
Ran for 1550 frames Cell Events: 60 mean_circ: 0.933563
Ran for 3050 frames Cell Events: 173 mean_circ: 0.935737
Ran for 3550 frames Cell Events: 193 mean_circ: 0.935747
Ran for 4050 frames Cell Events: 225 mean_circ: 0.93502
Ran for 4550 frames Cell Events: 260 mean_circ: 0.93501
Ran for 5050 frames Cell Events: 287 mean_circ: 0.934488
Ran for 6050 frames Cell Events: 329 mean_circ: 0.933696
Ran for 7050 frames Cell Events: 375 mean_circ: 0.932838
Ran for 9050 frames Cell Events: 485 mean_circ: 0.932945
Ran for 9550 frames Cell Events: 522 mean_circ: 0.932637
Ran for 10550 frames Cell Events: 574 mean_circ: 0.93363
Ran for 13050 frames Cell Events: 750 mean_circ: 0.934373
Ran for 14550 frames Cell Events: 848 mean_circ: 0.934293
Ran for 15550 frames Cell Events: 871 mean_circ: 0.93425
Ran for 17050 frames Cell Events: 950 mean_circ: 0.934342
Ran for 17550 frames Cell Events: 967 mean_circ: 0.93427
Ran for 18050 frames Cell Events: 1009 mean_circ: 0.934255
Ran for 18550 frames Cell Events: 1046 mean_circ: 0.934443
Ran for 19050 frames Cell Events: 1072 mean_circ: 0.934377
Ran for 20550 frames Cell Events: 1152 mean_circ: 0.934598
Ran for 21050 frames Cell Events: 1179 mean_circ: 0.934399
Ran for 21550 frames Cell Events: 1205 mean_circ: 0.934399
Ran for 22050 frames Cell Events: 1230 mean_circ: 0.934546
Ran for 22550 frames Cell Events: 1250 mean_circ: 0.934663
Ran for 23050 frames Cell Events: 1270 mean_circ: 0.934461
Ran for 23550 frames Cell Events: 1288 mean_circ: 0.934499
Ran for 24050 frames Cell Events: 1319 mean_circ: 0.934224
Ran for 24550 frames Cell Events: 1347 mean_circ: 0.934054
Ran for 25050 frames Cell Events: 1388 mean_circ: 0.934119
Ran for 26050 frames Cell Events: 1448 mean_circ: 0.933591
Ran for 27050 frames Cell Events: 1509 mean_circ: 0.93373
Ran for 29050 frames Cell Events: 1632 mean_circ: 0.933555
Ran for 29550 frames Cell Events: 1663 mean_circ: 0.933393
Ran for 30050 frames Cell Events: 1685 mean_circ: 0.933567
Ran for 30550 frames Cell Events: 1695 mean_circ: 0.933467
Ran for 32050 frames Cell Events: 1791 mean_circ: 0.933605
Ran for 33050 frames Cell Events: 1830 mean_circ: 0.933575
Ran for 33550 frames Cell Events: 1845 mean_circ: 0.933633
Ran for 35050 frames Cell Events: 1897 mean_circ: 0.933665
Ran for 36050 frames Cell Events: 1949 mean_circ: 0.933837
Ran for 36550 frames Cell Events: 1981 mean_circ: 0.93392
Ran for 37550 frames Cell Events: 2029 mean_circ: 0.934068
Ran for 38550 frames Cell Events: 2079 mean_circ: 0.933935
Ran for 40550 frames Cell Events: 2192 mean_circ: 0.934147
Ran for 41050 frames Cell Events: 2223 mean_circ: 0.934042
Ran for 41550 frames Cell Events: 2254 mean_circ: 0.934098
Ran for 44550 frames Cell Events: 2430 mean_circ: 0.934027
Ran for 45550 frames Cell Events: 2472 mean_circ: 0.934107
Ran for 46050 frames Cell Events: 2501 mean_circ: 0.934126
Ran for 46550 frames Cell Events: 2541 mean_circ: 0.934047
Ran for 47050 frames Cell Events: 2564 mean_circ: 0.934017
Ran for 51050 frames Cell Events: 2766 mean_circ: 0.933432
Ran for 53050 frames Cell Events: 2876 mean_circ: 0.93331
Ran for 53550 frames Cell Events: 2915 mean_circ: 0.933312
Ran for 57050 frames Cell Events: 3118 mean_circ: 0.933527
Ran for 57550 frames Cell Events: 3147 mean_circ: 0.933601
Ran for 61550 frames Cell Events: 3316 mean_circ: 0.93384
Ran for 62050 frames Cell Events: 3347 mean_circ: 0.933853
Ran for 64050 frames Cell Events: 3433 mean_circ: 0.933896
Ran for 64550 frames Cell Events: 3458 mean_circ: 0.933884
Ran for 65550 frames Cell Events: 3524 mean_circ: 0.933815
Ran for 66050 frames Cell Events: 3531 mean_circ: 0.933747
Ran for 68550 frames Cell Events: 3669 mean_circ: 0.933798
Ran for 74050 frames Cell Events: 3949 mean_circ: 0.934077
Ran for 75050 frames Cell Events: 4010 mean_circ: 0.934252
Ran for 75550 frames Cell Events: 4057 mean_circ: 0.934153
Ran for 78050 frames Cell Events: 4181 mean_circ: 0.934118
Ran for 78550 frames Cell Events: 4210 mean_circ: 0.934169
Ran for 79550 frames Cell Events: 4264 mean_circ: 0.934176
Ran for 80550 frames Cell Events: 4305 mean_circ: 0.934099
Ran for 82550 frames Cell Events: 4405 mean_circ: 0.934012
Ran for 83050 frames Cell Events: 4431 mean_circ: 0.93397
Ran for 83550 frames Cell Events: 4444 mean_circ: 0.933899
Ran for 84550 frames Cell Events: 4501 mean_circ: 0.933959
Ran for 85550 frames Cell Events: 4562 mean_circ: 0.934045
Ran for 86550 frames Cell Events: 4611 mean_circ: 0.934033
Ran for 87050 frames Cell Events: 4636 mean_circ: 0.934115
Ran for 87550 frames Cell Events: 4653 mean_circ: 0.934125
Ran for 88550 frames Cell Events: 4705 mean_circ: 0.934162
Ran for 89050 frames Cell Events: 4737 mean_circ: 0.934162
Ran for 90050 frames Cell Events: 4781 mean_circ: 0.93421
Ran for 90550 frames Cell Events: 4818 mean_circ: 0.93421
Ran for 91550 frames Cell Events: 4877 mean_circ: 0.934158
Ran for 94550 frames Cell Events: 5064 mean_circ: 0.934133
Ran for 97050 frames Cell Events: 5209 mean_circ: 0.934082
Ran for 99050 frames Cell Events: 5298 mean_circ: 0.934114
Ran for 102550 frames Cell Events: 5466 mean_circ: 0.933985
Ran for 103550 frames Cell Events: 5518 mean_circ: 0.934015
Ran for 104050 frames Cell Events: 5537 mean_circ: 0.934043
Ran for 106050 frames Cell Events: 5655 mean_circ: 0.934084
Ran for 107550 frames Cell Events: 5720 mean_circ: 0.934058
Ran for 108550 frames Cell Events: 5777 mean_circ: 0.933982
Ran for 109550 frames Cell Events: 5859 mean_circ: 0.933975
Ran for 113050 frames Cell Events: 6057 mean_circ: 0.933952
Ran for 114050 frames Cell Events: 6123 mean_circ: 0.934003
Ran for 115550 frames Cell Events: 6204 mean_circ: 0.934047
Ran for 116050 frames Cell Events: 6228 mean_circ: 0.934011
Ran for 117050 frames Cell Events: 6276 mean_circ: 0.934064
Ran for 121050 frames Cell Events: 6521 mean_circ: 0.933896
Ran for 121550 frames Cell Events: 6552 mean_circ: 0.933859
Ran for 122550 frames Cell Events: 6619 mean_circ: 0.933853
Ran for 123050 frames Cell Events: 6649 mean_circ: 0.933752
Ran for 124050 frames Cell Events: 6721 mean_circ: 0.933758
Ran for 124550 frames Cell Events: 6741 mean_circ: 0.93374
Ran for 126050 frames Cell Events: 6812 mean_circ: 0.93379
Ran for 127050 frames Cell Events: 6876 mean_circ: 0.933778
Ran for 127550 frames Cell Events: 6916 mean_circ: 0.933778
Ran for 128550 frames Cell Events: 6965 mean_circ: 0.933796
Ran for 129550 frames Cell Events: 7006 mean_circ: 0.933755
Ran for 130050 frames Cell Events: 7029 mean_circ: 0.933755
Ran for 131550 frames Cell Events: 7132 mean_circ: 0.933678
Ran for 133550 frames Cell Events: 7231 mean_circ: 0.933687
Ran for 134050 frames Cell Events: 7263 mean_circ: 0.933657
Ran for 134550 frames Cell Events: 7290 mean_circ: 0.933693
Ran for 135050 frames Cell Events: 7322 mean_circ: 0.933663
Ran for 137550 frames Cell Events: 7465 mean_circ: 0.933729
Ran for 138050 frames Cell Events: 7497 mean_circ: 0.93374
Ran for 138550 frames Cell Events: 7520 mean_circ: 0.933753
Ran for 140550 frames Cell Events: 7649 mean_circ: 0.933741
Ran for 142550 frames Cell Events: 7760 mean_circ: 0.933759
Ran for 143550 frames Cell Events: 7828 mean_circ: 0.9337
Ran for 144050 frames Cell Events: 7859 mean_circ: 0.933656
Ran for 144550 frames Cell Events: 7888 mean_circ: 0.93366
Ran for 148550 frames Cell Events: 8142 mean_circ: 0.933646
Ran for 149050 frames Cell Events: 8177 mean_circ: 0.933648
Ran for 149550 frames Cell Events: 8202 mean_circ: 0.933624
Ran for 150050 frames Cell Events: 8231 mean_circ: 0.933598
Ran for 151550 frames Cell Events: 8336 mean_circ: 0.933646
Ran for 153550 frames Cell Events: 8440 mean_circ: 0.933614
Ran for 156050 frames Cell Events: 8596 mean_circ: 0.933528
Ran for 156550 frames Cell Events: 8617 mean_circ: 0.933575
Ran for 157050 frames Cell Events: 8643 mean_circ: 0.933605
Ran for 157550 frames Cell Events: 8682 mean_circ: 0.93359
Ran for 158050 frames Cell Events: 8709 mean_circ: 0.933588
Ran for 158550 frames Cell Events: 8736 mean_circ: 0.933586
Ran for 159050 frames Cell Events: 8753 mean_circ: 0.933576
Ran for 159550 frames Cell Events: 8772 mean_circ: 0.933593
Ran for 160050 frames Cell Events: 8799 mean_circ: 0.933551
Ran for 162050 frames Cell Events: 8901 mean_circ: 0.933537
Ran for 162550 frames Cell Events: 8921 mean_circ: 0.933537
Ran for 163050 frames Cell Events: 8931 mean_circ: 0.933532
Ran for 165050 frames Cell Events: 9021 mean_circ: 0.933481
Ran for 165550 frames Cell Events: 9047 mean_circ: 0.933504
Ran for 166050 frames Cell Events: 9063 mean_circ: 0.933509
Ran for 167050 frames Cell Events: 9121 mean_circ: 0.933503
Ran for 167550 frames Cell Events: 9153 mean_circ: 0.933464
Ran for 168050 frames Cell Events: 9181 mean_circ: 0.933471
Ran for 169050 frames Cell Events: 9228 mean_circ: 0.933441
Ran for 171550 frames Cell Events: 9371 mean_circ: 0.933359
Ran for 172550 frames Cell Events: 9417 mean_circ: 0.933381
Ran for 173050 frames Cell Events: 9452 mean_circ: 0.933407
Ran for 173550 frames Cell Events: 9475 mean_circ: 0.933429
Ran for 174050 frames Cell Events: 9501 mean_circ: 0.933431



End this measurment..



Stopping time: 1.68156e+009
****************************************
Measurement finished

Mean Circularitiy: 0.933456
Mean Area: 650.108
Mean FL-1 intensity: 3704.29
Mean FL-2 intensity: 3810.61
Mean FL-3 intensity: 0

frames processed: 167990
Total Time: 87363.2ms
Number of Runs (new bg img) 0
Frames collected: 174588
Skipped frames: 164470
Rescued Frames: 4570204
Samples DAQ: 43646895
Samples written: 87293706
Samples per Channel: 43646853
FPS: 1998.42

Samples per second: 499602
Expected samples/s: 500000

Events: 9533
Events per second: 109.119
jumped back (PMT): 36
#Frames read, frequency
# 0 frames :279434
# 1 frames :55327
# 2 frames :2917
# 3 frames :1136
# 4 frames :744
# 5 frames :407
# 6 frames :1574
# 7 frames :1305
# 8 frames :1050
# 9 frames :729
# 10 frames :517
# 11 frames :439
# 12 frames :383
# 13 frames :262
# 14 frames :271
# 15 frames :238
# 16 frames :215
# 17 frames :186
# 18 frames :169
# 19 frames :123
# 20 frames :111
# 21 frames :94
# 22 frames :95
# 23 frames :77
# 24 frames :60
# 25 frames :67
# 26 frames :51
# 27 frames :57
# 28 frames :31
# 29 frames :41
# 30 frames :34
# 31 frames :27
# 32 frames :31
# 33 frames :20
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# 42 frames :5
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# 50 frames :7
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# 56 frames :7
# 57 frames :2
# 58 frames :1
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# 82 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.0103
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0