Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_12.25.54 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "6588417774be623ba986bd9ddfb32318",
"name": "M1_20170603_1939_dsRNA_D7_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "10:26:18"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 10000 (max 10001) in
'10000'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.0652
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.68218e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 64 mean_circ: 0.933829
Ran for 1550 frames Cell Events: 94 mean_circ: 0.937526
Ran for 2050 frames Cell Events: 125 mean_circ: 0.938636
Ran for 2550 frames Cell Events: 134 mean_circ: 0.939609
Ran for 3050 frames Cell Events: 156 mean_circ: 0.938809
Ran for 6050 frames Cell Events: 337 mean_circ: 0.936702
Ran for 7050 frames Cell Events: 365 mean_circ: 0.937171
Ran for 7550 frames Cell Events: 398 mean_circ: 0.936067
Ran for 8050 frames Cell Events: 433 mean_circ: 0.936134
Ran for 8550 frames Cell Events: 451 mean_circ: 0.936367
Ran for 9050 frames Cell Events: 475 mean_circ: 0.936582
Ran for 10550 frames Cell Events: 564 mean_circ: 0.936724
Ran for 13050 frames Cell Events: 729 mean_circ: 0.93635
Ran for 13550 frames Cell Events: 765 mean_circ: 0.936577
Ran for 14050 frames Cell Events: 801 mean_circ: 0.936577
Ran for 14550 frames Cell Events: 841 mean_circ: 0.936295
Ran for 15550 frames Cell Events: 905 mean_circ: 0.936667
Ran for 17050 frames Cell Events: 1006 mean_circ: 0.93715
Ran for 18050 frames Cell Events: 1062 mean_circ: 0.936742
Ran for 19050 frames Cell Events: 1125 mean_circ: 0.93695
Ran for 20050 frames Cell Events: 1204 mean_circ: 0.936841
Ran for 20550 frames Cell Events: 1238 mean_circ: 0.93688
Ran for 22050 frames Cell Events: 1345 mean_circ: 0.936883
Ran for 22550 frames Cell Events: 1377 mean_circ: 0.937092
Ran for 24550 frames Cell Events: 1512 mean_circ: 0.937106
Ran for 26550 frames Cell Events: 1635 mean_circ: 0.93673
Ran for 27050 frames Cell Events: 1666 mean_circ: 0.93675
Ran for 27550 frames Cell Events: 1696 mean_circ: 0.936738
Ran for 28550 frames Cell Events: 1757 mean_circ: 0.936783
Ran for 29050 frames Cell Events: 1794 mean_circ: 0.936998
Ran for 29550 frames Cell Events: 1812 mean_circ: 0.937031
Ran for 31050 frames Cell Events: 1912 mean_circ: 0.936988
Ran for 31550 frames Cell Events: 1940 mean_circ: 0.93709
Ran for 32050 frames Cell Events: 1966 mean_circ: 0.937088
Ran for 32550 frames Cell Events: 2012 mean_circ: 0.937088
Ran for 33050 frames Cell Events: 2045 mean_circ: 0.937272
Ran for 33550 frames Cell Events: 2079 mean_circ: 0.937316
Ran for 34050 frames Cell Events: 2113 mean_circ: 0.937477
Ran for 34550 frames Cell Events: 2145 mean_circ: 0.937489
Ran for 38550 frames Cell Events: 2367 mean_circ: 0.937498
Ran for 39550 frames Cell Events: 2403 mean_circ: 0.93764
Ran for 40550 frames Cell Events: 2472 mean_circ: 0.937519
Ran for 41550 frames Cell Events: 2537 mean_circ: 0.937532
Ran for 42050 frames Cell Events: 2552 mean_circ: 0.937537
Ran for 43050 frames Cell Events: 2610 mean_circ: 0.93757
Ran for 44050 frames Cell Events: 2671 mean_circ: 0.93769
Ran for 44550 frames Cell Events: 2700 mean_circ: 0.93756
Ran for 45550 frames Cell Events: 2741 mean_circ: 0.937676
Ran for 46050 frames Cell Events: 2766 mean_circ: 0.937687
Ran for 49050 frames Cell Events: 2960 mean_circ: 0.937389
Ran for 49550 frames Cell Events: 3002 mean_circ: 0.937356
Ran for 50050 frames Cell Events: 3040 mean_circ: 0.93728
Ran for 51550 frames Cell Events: 3126 mean_circ: 0.93722
Ran for 52550 frames Cell Events: 3165 mean_circ: 0.937104
Ran for 53050 frames Cell Events: 3198 mean_circ: 0.937042
Ran for 53550 frames Cell Events: 3239 mean_circ: 0.937091
Ran for 55550 frames Cell Events: 3362 mean_circ: 0.936846
Ran for 56550 frames Cell Events: 3425 mean_circ: 0.936838
Ran for 57050 frames Cell Events: 3464 mean_circ: 0.936911
Ran for 57550 frames Cell Events: 3491 mean_circ: 0.936903
Ran for 58050 frames Cell Events: 3519 mean_circ: 0.936918
Ran for 58550 frames Cell Events: 3546 mean_circ: 0.936907
Ran for 59050 frames Cell Events: 3584 mean_circ: 0.936894
Ran for 60050 frames Cell Events: 3642 mean_circ: 0.936811
Ran for 60550 frames Cell Events: 3676 mean_circ: 0.93685
Ran for 62050 frames Cell Events: 3780 mean_circ: 0.936567
Ran for 63550 frames Cell Events: 3864 mean_circ: 0.936624
Ran for 64050 frames Cell Events: 3894 mean_circ: 0.936654
Ran for 64550 frames Cell Events: 3913 mean_circ: 0.936723
Ran for 67050 frames Cell Events: 4048 mean_circ: 0.936635
Ran for 68550 frames Cell Events: 4139 mean_circ: 0.936606
Ran for 70550 frames Cell Events: 4248 mean_circ: 0.936639
Ran for 71050 frames Cell Events: 4268 mean_circ: 0.936626
Ran for 72050 frames Cell Events: 4334 mean_circ: 0.936656
Ran for 72550 frames Cell Events: 4356 mean_circ: 0.936655
Ran for 74050 frames Cell Events: 4456 mean_circ: 0.936515
Ran for 75050 frames Cell Events: 4514 mean_circ: 0.936515
Ran for 76550 frames Cell Events: 4622 mean_circ: 0.936537
Ran for 77050 frames Cell Events: 4654 mean_circ: 0.936516
Ran for 78050 frames Cell Events: 4712 mean_circ: 0.936586
Ran for 79050 frames Cell Events: 4769 mean_circ: 0.93666
Ran for 80050 frames Cell Events: 4831 mean_circ: 0.936712
Ran for 81550 frames Cell Events: 4897 mean_circ: 0.936631
Ran for 82550 frames Cell Events: 4944 mean_circ: 0.93661
Ran for 83050 frames Cell Events: 4982 mean_circ: 0.93661
Ran for 84050 frames Cell Events: 5046 mean_circ: 0.93657
Ran for 84550 frames Cell Events: 5069 mean_circ: 0.936555
Ran for 85550 frames Cell Events: 5110 mean_circ: 0.936561
Ran for 86050 frames Cell Events: 5138 mean_circ: 0.936562
Ran for 87550 frames Cell Events: 5202 mean_circ: 0.936531
Ran for 88550 frames Cell Events: 5253 mean_circ: 0.936532
Ran for 89550 frames Cell Events: 5298 mean_circ: 0.9365
Ran for 90550 frames Cell Events: 5356 mean_circ: 0.936406
Ran for 91050 frames Cell Events: 5390 mean_circ: 0.93642
Ran for 91550 frames Cell Events: 5422 mean_circ: 0.936448
Ran for 92550 frames Cell Events: 5474 mean_circ: 0.936408
Ran for 93050 frames Cell Events: 5493 mean_circ: 0.93644
Ran for 93550 frames Cell Events: 5520 mean_circ: 0.936489
Ran for 94050 frames Cell Events: 5554 mean_circ: 0.936532
Ran for 96550 frames Cell Events: 5708 mean_circ: 0.936513
Ran for 97550 frames Cell Events: 5763 mean_circ: 0.936546
Ran for 98050 frames Cell Events: 5780 mean_circ: 0.936593
Ran for 99050 frames Cell Events: 5841 mean_circ: 0.93654
Ran for 100550 frames Cell Events: 5937 mean_circ: 0.936569
Ran for 101050 frames Cell Events: 5964 mean_circ: 0.936565
Ran for 101550 frames Cell Events: 5989 mean_circ: 0.936567
Ran for 102050 frames Cell Events: 6013 mean_circ: 0.936544
Ran for 102550 frames Cell Events: 6045 mean_circ: 0.936563
Ran for 103050 frames Cell Events: 6070 mean_circ: 0.936564
Ran for 103550 frames Cell Events: 6106 mean_circ: 0.936563
Ran for 104050 frames Cell Events: 6139 mean_circ: 0.936525
Ran for 104550 frames Cell Events: 6177 mean_circ: 0.936525
Ran for 105050 frames Cell Events: 6208 mean_circ: 0.936457
Ran for 106050 frames Cell Events: 6238 mean_circ: 0.936528
Ran for 108550 frames Cell Events: 6400 mean_circ: 0.936414
Ran for 109050 frames Cell Events: 6425 mean_circ: 0.936407
Ran for 109550 frames Cell Events: 6449 mean_circ: 0.936433
Ran for 111050 frames Cell Events: 6515 mean_circ: 0.936469
Ran for 111550 frames Cell Events: 6543 mean_circ: 0.936526
Ran for 112050 frames Cell Events: 6567 mean_circ: 0.93654
Ran for 112550 frames Cell Events: 6607 mean_circ: 0.936532
Ran for 113050 frames Cell Events: 6629 mean_circ: 0.936478
Ran for 113550 frames Cell Events: 6657 mean_circ: 0.936511
Ran for 114050 frames Cell Events: 6690 mean_circ: 0.936511
Ran for 114550 frames Cell Events: 6709 mean_circ: 0.936441
Ran for 118050 frames Cell Events: 6908 mean_circ: 0.936321
Ran for 118550 frames Cell Events: 6934 mean_circ: 0.936323
Ran for 119050 frames Cell Events: 6966 mean_circ: 0.936384
Ran for 120050 frames Cell Events: 7044 mean_circ: 0.936384
Ran for 121050 frames Cell Events: 7105 mean_circ: 0.93629
Ran for 122550 frames Cell Events: 7191 mean_circ: 0.936206
Ran for 126050 frames Cell Events: 7428 mean_circ: 0.93611
Ran for 126550 frames Cell Events: 7461 mean_circ: 0.936126
Ran for 127050 frames Cell Events: 7489 mean_circ: 0.936159
Ran for 127550 frames Cell Events: 7521 mean_circ: 0.936178
Ran for 128050 frames Cell Events: 7549 mean_circ: 0.936178
Ran for 129550 frames Cell Events: 7651 mean_circ: 0.936153
Ran for 131050 frames Cell Events: 7768 mean_circ: 0.936165
Ran for 132550 frames Cell Events: 7862 mean_circ: 0.936227
Ran for 133050 frames Cell Events: 7900 mean_circ: 0.93618
Ran for 134050 frames Cell Events: 7967 mean_circ: 0.936229
Ran for 135550 frames Cell Events: 8041 mean_circ: 0.936201
Ran for 139050 frames Cell Events: 8307 mean_circ: 0.936098
Ran for 139550 frames Cell Events: 8323 mean_circ: 0.936068
Ran for 140050 frames Cell Events: 8340 mean_circ: 0.936089
Ran for 140550 frames Cell Events: 8388 mean_circ: 0.936089
Ran for 141050 frames Cell Events: 8422 mean_circ: 0.936067
Ran for 141550 frames Cell Events: 8452 mean_circ: 0.936105
Ran for 142050 frames Cell Events: 8477 mean_circ: 0.936119
Ran for 143050 frames Cell Events: 8546 mean_circ: 0.936172
Ran for 143550 frames Cell Events: 8588 mean_circ: 0.936172
Ran for 145050 frames Cell Events: 8649 mean_circ: 0.936226
Ran for 145550 frames Cell Events: 8675 mean_circ: 0.936158
Ran for 146050 frames Cell Events: 8710 mean_circ: 0.93615
Ran for 146550 frames Cell Events: 8745 mean_circ: 0.936141
Ran for 147550 frames Cell Events: 8835 mean_circ: 0.93615
Ran for 149050 frames Cell Events: 8925 mean_circ: 0.936165
Ran for 149550 frames Cell Events: 8955 mean_circ: 0.936166
Ran for 150050 frames Cell Events: 8981 mean_circ: 0.936195
Ran for 150550 frames Cell Events: 9007 mean_circ: 0.936168
Ran for 152050 frames Cell Events: 9109 mean_circ: 0.93621
Ran for 153550 frames Cell Events: 9149 mean_circ: 0.936191
Ran for 155550 frames Cell Events: 9288 mean_circ: 0.936188
Ran for 157550 frames Cell Events: 9442 mean_circ: 0.936232
Ran for 158550 frames Cell Events: 9489 mean_circ: 0.936254
Ran for 159050 frames Cell Events: 9521 mean_circ: 0.936259
Ran for 159550 frames Cell Events: 9538 mean_circ: 0.93629
Ran for 160050 frames Cell Events: 9549 mean_circ: 0.936317
Ran for 160550 frames Cell Events: 9580 mean_circ: 0.93632
Ran for 161050 frames Cell Events: 9612 mean_circ: 0.936297
Ran for 161550 frames Cell Events: 9636 mean_circ: 0.936279
Ran for 162050 frames Cell Events: 9655 mean_circ: 0.936253
Ran for 163050 frames Cell Events: 9714 mean_circ: 0.936222
Ran for 163550 frames Cell Events: 9738 mean_circ: 0.936199
Ran for 164550 frames Cell Events: 9807 mean_circ: 0.936226
Ran for 165050 frames Cell Events: 9836 mean_circ: 0.936258
Ran for 165550 frames Cell Events: 9854 mean_circ: 0.936219
Ran for 167050 frames Cell Events: 9952 mean_circ: 0.936212



End this measurment.
because stop condition (time/nr of cells) was met

Stopping time: 1.68226e+009
****************************************
Measurement finished

Mean Circularitiy: 0.936247
Mean Area: 605.06
Mean FL-1 intensity: 3568.47
Mean FL-2 intensity: 3297.77
Mean FL-3 intensity: 0

frames processed: 163159
Total Time: 84019.8ms
Number of Runs (new bg img) 0
Frames collected: 167896
Skipped frames: 171736
Rescued Frames: 4736601
Samples DAQ: 41975075
Samples written: 83947782
Samples per Channel: 41973891
FPS: 1998.29

Samples per second: 499572
Expected samples/s: 500000

Events: 10001
Events per second: 119.031
jumped back (PMT): 20
#Frames read, frequency
# 0 frames :257884
# 1 frames :63747
# 2 frames :3106
# 3 frames :1191
# 4 frames :534
# 5 frames :270
# 6 frames :3086
# 7 frames :1343
# 8 frames :1232
# 9 frames :642
# 10 frames :445
# 11 frames :306
# 12 frames :262
# 13 frames :213
# 14 frames :322
# 15 frames :201
# 16 frames :152
# 17 frames :116
# 18 frames :98
# 19 frames :59
# 20 frames :76
# 21 frames :63
# 22 frames :50
# 23 frames :49
# 24 frames :39
# 25 frames :40
# 26 frames :25
# 27 frames :21
# 28 frames :30
# 29 frames :15
# 30 frames :16
# 31 frames :15
# 32 frames :14
# 33 frames :11
# 34 frames :16
# 35 frames :6
# 36 frames :8
# 37 frames :2
# 38 frames :7
# 39 frames :3
# 40 frames :4
# 41 frames :7
# 42 frames :7
# 43 frames :2
# 44 frames :1
# 45 frames :1
# 47 frames :6
# 48 frames :1
# 50 frames :1
# 51 frames :4
# 52 frames :3
# 53 frames :4
# 62 frames :1
# 63 frames :1
# 67 frames :1
# 68 frames :1
# 72 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.0652
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0