Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_12.32.35 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "2f5c94e7d389c49bdd006270ecdbf214",
"name": "M1_20170604_1416_NK1_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "10:32:56"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8187 (max 8188) in '8187'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.5657
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.74922e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 46 mean_circ: 0.94001
Ran for 1550 frames Cell Events: 62 mean_circ: 0.935639
Ran for 2050 frames Cell Events: 87 mean_circ: 0.937477
Ran for 2550 frames Cell Events: 105 mean_circ: 0.937629
Ran for 5050 frames Cell Events: 246 mean_circ: 0.934869
Ran for 5550 frames Cell Events: 282 mean_circ: 0.933729
Ran for 6050 frames Cell Events: 309 mean_circ: 0.933129
Ran for 7550 frames Cell Events: 390 mean_circ: 0.933527
Ran for 8050 frames Cell Events: 409 mean_circ: 0.933213
Ran for 8550 frames Cell Events: 427 mean_circ: 0.933375
Ran for 9050 frames Cell Events: 442 mean_circ: 0.933299
Ran for 10550 frames Cell Events: 508 mean_circ: 0.933237
Ran for 11050 frames Cell Events: 534 mean_circ: 0.933221
Ran for 12050 frames Cell Events: 576 mean_circ: 0.933705
Ran for 12550 frames Cell Events: 606 mean_circ: 0.933462
Ran for 13550 frames Cell Events: 656 mean_circ: 0.933315
Ran for 15550 frames Cell Events: 749 mean_circ: 0.932826
Ran for 17050 frames Cell Events: 794 mean_circ: 0.932457
Ran for 18550 frames Cell Events: 875 mean_circ: 0.931897
Ran for 19050 frames Cell Events: 898 mean_circ: 0.931857
Ran for 19550 frames Cell Events: 916 mean_circ: 0.931821
Ran for 20050 frames Cell Events: 931 mean_circ: 0.931968
Ran for 20550 frames Cell Events: 944 mean_circ: 0.932067
Ran for 22050 frames Cell Events: 1005 mean_circ: 0.932025
Ran for 22550 frames Cell Events: 1026 mean_circ: 0.932076
Ran for 23550 frames Cell Events: 1074 mean_circ: 0.93188
Ran for 25050 frames Cell Events: 1140 mean_circ: 0.931263
Ran for 26050 frames Cell Events: 1175 mean_circ: 0.930857
Ran for 26550 frames Cell Events: 1206 mean_circ: 0.930681
Ran for 27050 frames Cell Events: 1236 mean_circ: 0.930507
Ran for 27550 frames Cell Events: 1251 mean_circ: 0.930646
Ran for 28050 frames Cell Events: 1275 mean_circ: 0.930526
Ran for 28550 frames Cell Events: 1294 mean_circ: 0.930711
Ran for 29050 frames Cell Events: 1324 mean_circ: 0.930649
Ran for 29550 frames Cell Events: 1347 mean_circ: 0.930663
Ran for 30050 frames Cell Events: 1369 mean_circ: 0.930559
Ran for 30550 frames Cell Events: 1390 mean_circ: 0.930366
Ran for 33050 frames Cell Events: 1489 mean_circ: 0.930571
Ran for 34050 frames Cell Events: 1526 mean_circ: 0.930674
Ran for 34550 frames Cell Events: 1544 mean_circ: 0.930577
Ran for 35550 frames Cell Events: 1587 mean_circ: 0.930717
Ran for 36550 frames Cell Events: 1630 mean_circ: 0.930691
Ran for 37550 frames Cell Events: 1686 mean_circ: 0.930559
Ran for 38050 frames Cell Events: 1703 mean_circ: 0.930512
Ran for 38550 frames Cell Events: 1728 mean_circ: 0.930586
Ran for 39050 frames Cell Events: 1748 mean_circ: 0.930465
Ran for 39550 frames Cell Events: 1769 mean_circ: 0.930432
Ran for 40550 frames Cell Events: 1808 mean_circ: 0.930579
Ran for 41550 frames Cell Events: 1855 mean_circ: 0.9307
Ran for 42050 frames Cell Events: 1874 mean_circ: 0.930597
Ran for 42550 frames Cell Events: 1900 mean_circ: 0.930597
Ran for 43050 frames Cell Events: 1919 mean_circ: 0.930712
Ran for 43550 frames Cell Events: 1936 mean_circ: 0.930655
Ran for 44050 frames Cell Events: 1956 mean_circ: 0.930695
Ran for 44550 frames Cell Events: 1983 mean_circ: 0.930598
Ran for 47050 frames Cell Events: 2094 mean_circ: 0.93047
Ran for 48550 frames Cell Events: 2178 mean_circ: 0.93021
Ran for 49050 frames Cell Events: 2192 mean_circ: 0.930219
Ran for 49550 frames Cell Events: 2216 mean_circ: 0.930191
Ran for 53050 frames Cell Events: 2382 mean_circ: 0.930157
Ran for 53550 frames Cell Events: 2411 mean_circ: 0.930254
Ran for 54550 frames Cell Events: 2466 mean_circ: 0.92999
Ran for 55050 frames Cell Events: 2481 mean_circ: 0.929942
Ran for 57550 frames Cell Events: 2609 mean_circ: 0.929798
Ran for 59050 frames Cell Events: 2677 mean_circ: 0.929768
Ran for 60550 frames Cell Events: 2767 mean_circ: 0.929576
Ran for 61050 frames Cell Events: 2794 mean_circ: 0.929536
Ran for 62550 frames Cell Events: 2889 mean_circ: 0.929578
Ran for 63550 frames Cell Events: 2937 mean_circ: 0.929527
Ran for 64050 frames Cell Events: 2959 mean_circ: 0.929493
Ran for 64550 frames Cell Events: 2980 mean_circ: 0.929413
Ran for 66050 frames Cell Events: 3048 mean_circ: 0.929363
Ran for 66550 frames Cell Events: 3064 mean_circ: 0.929351
Ran for 68050 frames Cell Events: 3145 mean_circ: 0.929363
Ran for 69550 frames Cell Events: 3227 mean_circ: 0.929531
Ran for 70050 frames Cell Events: 3242 mean_circ: 0.929483
Ran for 70550 frames Cell Events: 3276 mean_circ: 0.929541
Ran for 71550 frames Cell Events: 3319 mean_circ: 0.929519
Ran for 72050 frames Cell Events: 3337 mean_circ: 0.929513
Ran for 73550 frames Cell Events: 3396 mean_circ: 0.929479
Ran for 75550 frames Cell Events: 3500 mean_circ: 0.929677
Ran for 76550 frames Cell Events: 3537 mean_circ: 0.929765
Ran for 77550 frames Cell Events: 3573 mean_circ: 0.92971
Ran for 78050 frames Cell Events: 3595 mean_circ: 0.929725
Ran for 79050 frames Cell Events: 3637 mean_circ: 0.929641
Ran for 81550 frames Cell Events: 3751 mean_circ: 0.929567
Ran for 83050 frames Cell Events: 3830 mean_circ: 0.929595
Ran for 85050 frames Cell Events: 3928 mean_circ: 0.929604
Ran for 85550 frames Cell Events: 3947 mean_circ: 0.929664
Ran for 86550 frames Cell Events: 3982 mean_circ: 0.929643
Ran for 87550 frames Cell Events: 4032 mean_circ: 0.929797
Ran for 89550 frames Cell Events: 4135 mean_circ: 0.929672
Ran for 90050 frames Cell Events: 4165 mean_circ: 0.929769
Ran for 91050 frames Cell Events: 4209 mean_circ: 0.929771
Ran for 91550 frames Cell Events: 4230 mean_circ: 0.929726
Ran for 92050 frames Cell Events: 4252 mean_circ: 0.92973
Ran for 92550 frames Cell Events: 4286 mean_circ: 0.929714
Ran for 93050 frames Cell Events: 4304 mean_circ: 0.929711
Ran for 94050 frames Cell Events: 4359 mean_circ: 0.929818
Ran for 94550 frames Cell Events: 4399 mean_circ: 0.929779
Ran for 96550 frames Cell Events: 4492 mean_circ: 0.929825
Ran for 98050 frames Cell Events: 4551 mean_circ: 0.929762
Ran for 98550 frames Cell Events: 4575 mean_circ: 0.929759
Ran for 99550 frames Cell Events: 4619 mean_circ: 0.929697
Ran for 101550 frames Cell Events: 4746 mean_circ: 0.92979
Ran for 103050 frames Cell Events: 4829 mean_circ: 0.929639
Ran for 105050 frames Cell Events: 4917 mean_circ: 0.929706
Ran for 105550 frames Cell Events: 4944 mean_circ: 0.929665
Ran for 106050 frames Cell Events: 4970 mean_circ: 0.929554
Ran for 106550 frames Cell Events: 4984 mean_circ: 0.929544
Ran for 107050 frames Cell Events: 5016 mean_circ: 0.929534
Ran for 108050 frames Cell Events: 5069 mean_circ: 0.929619
Ran for 112050 frames Cell Events: 5291 mean_circ: 0.929411
Ran for 112550 frames Cell Events: 5308 mean_circ: 0.929436
Ran for 113050 frames Cell Events: 5333 mean_circ: 0.929428
Ran for 113550 frames Cell Events: 5361 mean_circ: 0.929444
Ran for 114550 frames Cell Events: 5417 mean_circ: 0.929483
Ran for 115050 frames Cell Events: 5432 mean_circ: 0.929504
Ran for 115550 frames Cell Events: 5464 mean_circ: 0.929533
Ran for 116550 frames Cell Events: 5504 mean_circ: 0.929611
Ran for 117050 frames Cell Events: 5535 mean_circ: 0.929651
Ran for 118050 frames Cell Events: 5577 mean_circ: 0.929698
Ran for 118550 frames Cell Events: 5603 mean_circ: 0.929616
Ran for 119550 frames Cell Events: 5653 mean_circ: 0.929685
Ran for 120550 frames Cell Events: 5701 mean_circ: 0.929676
Ran for 121050 frames Cell Events: 5725 mean_circ: 0.929682
Ran for 121550 frames Cell Events: 5737 mean_circ: 0.929702
Ran for 122050 frames Cell Events: 5750 mean_circ: 0.929671
Ran for 125050 frames Cell Events: 5908 mean_circ: 0.929621
Ran for 125550 frames Cell Events: 5937 mean_circ: 0.929575
Ran for 127050 frames Cell Events: 6004 mean_circ: 0.929621
Ran for 129550 frames Cell Events: 6126 mean_circ: 0.92963
Ran for 131550 frames Cell Events: 6240 mean_circ: 0.929494
Ran for 134050 frames Cell Events: 6362 mean_circ: 0.9296
Ran for 136050 frames Cell Events: 6468 mean_circ: 0.929545
Ran for 137050 frames Cell Events: 6510 mean_circ: 0.929556
Ran for 139050 frames Cell Events: 6618 mean_circ: 0.929506
Ran for 141050 frames Cell Events: 6715 mean_circ: 0.929491
Ran for 142550 frames Cell Events: 6787 mean_circ: 0.929469
Ran for 145050 frames Cell Events: 6936 mean_circ: 0.92932
Ran for 146550 frames Cell Events: 7006 mean_circ: 0.929309
Ran for 147550 frames Cell Events: 7053 mean_circ: 0.929292
Ran for 150550 frames Cell Events: 7201 mean_circ: 0.929173
Ran for 151550 frames Cell Events: 7260 mean_circ: 0.929199
Ran for 152550 frames Cell Events: 7309 mean_circ: 0.929155
Ran for 153050 frames Cell Events: 7329 mean_circ: 0.929175
Ran for 153550 frames Cell Events: 7353 mean_circ: 0.929177
Ran for 154050 frames Cell Events: 7379 mean_circ: 0.929116
Ran for 154550 frames Cell Events: 7396 mean_circ: 0.929116
Ran for 155050 frames Cell Events: 7426 mean_circ: 0.929112
Ran for 155550 frames Cell Events: 7450 mean_circ: 0.929122
Ran for 156550 frames Cell Events: 7481 mean_circ: 0.929077
Ran for 157050 frames Cell Events: 7501 mean_circ: 0.929084
Ran for 158050 frames Cell Events: 7543 mean_circ: 0.929105
Ran for 160550 frames Cell Events: 7690 mean_circ: 0.929088
Ran for 161050 frames Cell Events: 7714 mean_circ: 0.929102
Ran for 161550 frames Cell Events: 7730 mean_circ: 0.929093
Ran for 162050 frames Cell Events: 7764 mean_circ: 0.929093
Ran for 163050 frames Cell Events: 7809 mean_circ: 0.929068
Ran for 163550 frames Cell Events: 7838 mean_circ: 0.929033
Ran for 164550 frames Cell Events: 7884 mean_circ: 0.929069
Ran for 165050 frames Cell Events: 7918 mean_circ: 0.929049
Ran for 165550 frames Cell Events: 7946 mean_circ: 0.929011
Ran for 166050 frames Cell Events: 7962 mean_circ: 0.928993
Ran for 167550 frames Cell Events: 8035 mean_circ: 0.928994
Ran for 169550 frames Cell Events: 8130 mean_circ: 0.929146
Ran for 170050 frames Cell Events: 8148 mean_circ: 0.929127



End this measurment..



Stopping time: 1.7493e+009
****************************************
Measurement finished

Mean Circularitiy: 0.929125
Mean Area: 621.467
Mean FL-1 intensity: 3071.31
Mean FL-2 intensity: 4383.13
Mean FL-3 intensity: 0

frames processed: 167640
Total Time: 85369.8ms
Number of Runs (new bg img) 0
Frames collected: 170614
Skipped frames: 7874
Rescued Frames: 216291
Samples DAQ: 42657092
Samples written: 85307074
Samples per Channel: 42653537
FPS: 1998.53

Samples per second: 499633
Expected samples/s: 500000

Events: 8188
Events per second: 95.9122
jumped back (PMT): 19
#Frames read, frequency
# 0 frames :269499
# 1 frames :57866
# 2 frames :2996
# 3 frames :1220
# 4 frames :748
# 5 frames :442
# 6 frames :1007
# 7 frames :1227
# 8 frames :1000
# 9 frames :679
# 10 frames :609
# 11 frames :526
# 12 frames :404
# 13 frames :298
# 14 frames :260
# 15 frames :201
# 16 frames :190
# 17 frames :162
# 18 frames :163
# 19 frames :144
# 20 frames :142
# 21 frames :113
# 22 frames :103
# 23 frames :91
# 24 frames :65
# 25 frames :54
# 26 frames :39
# 27 frames :51
# 28 frames :39
# 29 frames :40
# 30 frames :31
# 31 frames :30
# 32 frames :27
# 33 frames :24
# 34 frames :10
# 35 frames :22
# 36 frames :16
# 37 frames :20
# 38 frames :7
# 39 frames :11
# 40 frames :15
# 41 frames :7
# 42 frames :11
# 43 frames :9
# 44 frames :7
# 45 frames :4
# 46 frames :7
# 47 frames :10
# 48 frames :7
# 49 frames :3
# 50 frames :4
# 51 frames :2
# 52 frames :3
# 53 frames :3
# 54 frames :5
# 55 frames :1
# 56 frames :1
# 57 frames :3
# 58 frames :2
# 59 frames :2
# 60 frames :5
# 61 frames :1
# 62 frames :1
# 68 frames :1
# 72 frames :1
# 74 frames :1
# 84 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.5657
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0