Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_12.33.03 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "01d1cbfce02949a1ceb8e7f8d1a8b2fa",
"name": "M1_20170604_1424_dsRNA_E1_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "10:33:28"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 10000 (max 10001) in
'10000'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.5951
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.74972e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 27 mean_circ: 0.947169
Ran for 1050 frames Cell Events: 44 mean_circ: 0.950244
Ran for 2050 frames Cell Events: 85 mean_circ: 0.944639
Ran for 2550 frames Cell Events: 114 mean_circ: 0.942686
Ran for 3050 frames Cell Events: 143 mean_circ: 0.942686
Ran for 3550 frames Cell Events: 155 mean_circ: 0.941559
Ran for 5550 frames Cell Events: 230 mean_circ: 0.941403
Ran for 6050 frames Cell Events: 255 mean_circ: 0.942106
Ran for 7050 frames Cell Events: 318 mean_circ: 0.94172
Ran for 7550 frames Cell Events: 358 mean_circ: 0.941834
Ran for 10550 frames Cell Events: 463 mean_circ: 0.942805
Ran for 11050 frames Cell Events: 489 mean_circ: 0.942384
Ran for 11550 frames Cell Events: 503 mean_circ: 0.942423
Ran for 12050 frames Cell Events: 520 mean_circ: 0.942905
Ran for 12550 frames Cell Events: 548 mean_circ: 0.943328
Ran for 13050 frames Cell Events: 572 mean_circ: 0.942979
Ran for 14050 frames Cell Events: 624 mean_circ: 0.942
Ran for 14550 frames Cell Events: 652 mean_circ: 0.942249
Ran for 15050 frames Cell Events: 666 mean_circ: 0.941598
Ran for 15550 frames Cell Events: 687 mean_circ: 0.941727
Ran for 16550 frames Cell Events: 716 mean_circ: 0.941829
Ran for 17050 frames Cell Events: 742 mean_circ: 0.941944
Ran for 17550 frames Cell Events: 761 mean_circ: 0.94227
Ran for 18050 frames Cell Events: 787 mean_circ: 0.942344
Ran for 18550 frames Cell Events: 795 mean_circ: 0.942547
Ran for 19550 frames Cell Events: 856 mean_circ: 0.942246
Ran for 20050 frames Cell Events: 880 mean_circ: 0.942416
Ran for 21550 frames Cell Events: 951 mean_circ: 0.942617
Ran for 22550 frames Cell Events: 1003 mean_circ: 0.942023
Ran for 23550 frames Cell Events: 1045 mean_circ: 0.942608
Ran for 24550 frames Cell Events: 1093 mean_circ: 0.942735
Ran for 25050 frames Cell Events: 1114 mean_circ: 0.942994
Ran for 25550 frames Cell Events: 1135 mean_circ: 0.942966
Ran for 26050 frames Cell Events: 1158 mean_circ: 0.942966
Ran for 27050 frames Cell Events: 1208 mean_circ: 0.942994
Ran for 28050 frames Cell Events: 1260 mean_circ: 0.943159
Ran for 30050 frames Cell Events: 1375 mean_circ: 0.944043
Ran for 31050 frames Cell Events: 1407 mean_circ: 0.943929
Ran for 31550 frames Cell Events: 1423 mean_circ: 0.943921
Ran for 32050 frames Cell Events: 1443 mean_circ: 0.943921
Ran for 33550 frames Cell Events: 1522 mean_circ: 0.944083
Ran for 36550 frames Cell Events: 1655 mean_circ: 0.944502
Ran for 37550 frames Cell Events: 1694 mean_circ: 0.944473
Ran for 40050 frames Cell Events: 1838 mean_circ: 0.944703
Ran for 40550 frames Cell Events: 1863 mean_circ: 0.944766
Ran for 43550 frames Cell Events: 1970 mean_circ: 0.94466
Ran for 44050 frames Cell Events: 1997 mean_circ: 0.944534
Ran for 45550 frames Cell Events: 2056 mean_circ: 0.944558
Ran for 46550 frames Cell Events: 2095 mean_circ: 0.944697
Ran for 47550 frames Cell Events: 2142 mean_circ: 0.944854
Ran for 48550 frames Cell Events: 2191 mean_circ: 0.945068
Ran for 49550 frames Cell Events: 2223 mean_circ: 0.945212
Ran for 52050 frames Cell Events: 2340 mean_circ: 0.945333
Ran for 52550 frames Cell Events: 2357 mean_circ: 0.9454
Ran for 53050 frames Cell Events: 2380 mean_circ: 0.94551
Ran for 54050 frames Cell Events: 2426 mean_circ: 0.945498
Ran for 54550 frames Cell Events: 2445 mean_circ: 0.945538
Ran for 55050 frames Cell Events: 2468 mean_circ: 0.945645
Ran for 56550 frames Cell Events: 2528 mean_circ: 0.945819
Ran for 58050 frames Cell Events: 2606 mean_circ: 0.945751
Ran for 58550 frames Cell Events: 2625 mean_circ: 0.945694
Ran for 59550 frames Cell Events: 2663 mean_circ: 0.945722
Ran for 63050 frames Cell Events: 2856 mean_circ: 0.945979
Ran for 63550 frames Cell Events: 2879 mean_circ: 0.945992
Ran for 65050 frames Cell Events: 2960 mean_circ: 0.946008
Ran for 65550 frames Cell Events: 2983 mean_circ: 0.946033
Ran for 66550 frames Cell Events: 3042 mean_circ: 0.946036
Ran for 67050 frames Cell Events: 3059 mean_circ: 0.946054
Ran for 69050 frames Cell Events: 3127 mean_circ: 0.94593
Ran for 69550 frames Cell Events: 3151 mean_circ: 0.946001
Ran for 71050 frames Cell Events: 3223 mean_circ: 0.946097
Ran for 74550 frames Cell Events: 3408 mean_circ: 0.946015
Ran for 76550 frames Cell Events: 3519 mean_circ: 0.946103
Ran for 79550 frames Cell Events: 3701 mean_circ: 0.94624
Ran for 80050 frames Cell Events: 3733 mean_circ: 0.946228
Ran for 81550 frames Cell Events: 3813 mean_circ: 0.946382
Ran for 82050 frames Cell Events: 3845 mean_circ: 0.946326
Ran for 82550 frames Cell Events: 3870 mean_circ: 0.946213
Ran for 83550 frames Cell Events: 3917 mean_circ: 0.946233
Ran for 84050 frames Cell Events: 3932 mean_circ: 0.946208
Ran for 85050 frames Cell Events: 3985 mean_circ: 0.946206
Ran for 86050 frames Cell Events: 4034 mean_circ: 0.946153
Ran for 87550 frames Cell Events: 4091 mean_circ: 0.946184
Ran for 89550 frames Cell Events: 4179 mean_circ: 0.946371
Ran for 90050 frames Cell Events: 4198 mean_circ: 0.946371
Ran for 91550 frames Cell Events: 4290 mean_circ: 0.946386
Ran for 92050 frames Cell Events: 4308 mean_circ: 0.946335
Ran for 92550 frames Cell Events: 4345 mean_circ: 0.946205
Ran for 93050 frames Cell Events: 4380 mean_circ: 0.946118
Ran for 93550 frames Cell Events: 4413 mean_circ: 0.946201
Ran for 95050 frames Cell Events: 4479 mean_circ: 0.946163
Ran for 95550 frames Cell Events: 4509 mean_circ: 0.946151
Ran for 96550 frames Cell Events: 4537 mean_circ: 0.946148
Ran for 98050 frames Cell Events: 4604 mean_circ: 0.946244
Ran for 99050 frames Cell Events: 4658 mean_circ: 0.94625
Ran for 99550 frames Cell Events: 4678 mean_circ: 0.94622
Ran for 101550 frames Cell Events: 4769 mean_circ: 0.946229
Ran for 102550 frames Cell Events: 4810 mean_circ: 0.946249
Ran for 103550 frames Cell Events: 4850 mean_circ: 0.946293
Ran for 105550 frames Cell Events: 4938 mean_circ: 0.946327
Ran for 108050 frames Cell Events: 5044 mean_circ: 0.946178
Ran for 109050 frames Cell Events: 5093 mean_circ: 0.94616
Ran for 111550 frames Cell Events: 5230 mean_circ: 0.946132
Ran for 112550 frames Cell Events: 5276 mean_circ: 0.946147
Ran for 115050 frames Cell Events: 5425 mean_circ: 0.946134
Ran for 115550 frames Cell Events: 5447 mean_circ: 0.946173
Ran for 116550 frames Cell Events: 5494 mean_circ: 0.946213
Ran for 118050 frames Cell Events: 5551 mean_circ: 0.946242
Ran for 118550 frames Cell Events: 5571 mean_circ: 0.946242
Ran for 120550 frames Cell Events: 5680 mean_circ: 0.946141
Ran for 121550 frames Cell Events: 5712 mean_circ: 0.946164
Ran for 122050 frames Cell Events: 5743 mean_circ: 0.946223
Ran for 122550 frames Cell Events: 5779 mean_circ: 0.946244
Ran for 124050 frames Cell Events: 5847 mean_circ: 0.946315
Ran for 125050 frames Cell Events: 5886 mean_circ: 0.946343
Ran for 126050 frames Cell Events: 5929 mean_circ: 0.946338
Ran for 128550 frames Cell Events: 6039 mean_circ: 0.946255
Ran for 129050 frames Cell Events: 6074 mean_circ: 0.946208
Ran for 129550 frames Cell Events: 6097 mean_circ: 0.94624
Ran for 130050 frames Cell Events: 6113 mean_circ: 0.946222
Ran for 130550 frames Cell Events: 6132 mean_circ: 0.946227
Ran for 132050 frames Cell Events: 6198 mean_circ: 0.946173
Ran for 133050 frames Cell Events: 6251 mean_circ: 0.946149
Ran for 134050 frames Cell Events: 6299 mean_circ: 0.946052
Ran for 134550 frames Cell Events: 6320 mean_circ: 0.946004
Ran for 135050 frames Cell Events: 6343 mean_circ: 0.946007
Ran for 135550 frames Cell Events: 6360 mean_circ: 0.946051
Ran for 136550 frames Cell Events: 6409 mean_circ: 0.946031
Ran for 137050 frames Cell Events: 6425 mean_circ: 0.946052
Ran for 137550 frames Cell Events: 6447 mean_circ: 0.946033
Ran for 139050 frames Cell Events: 6512 mean_circ: 0.946047
Ran for 140050 frames Cell Events: 6554 mean_circ: 0.946018
Ran for 140550 frames Cell Events: 6574 mean_circ: 0.94607
Ran for 141050 frames Cell Events: 6599 mean_circ: 0.946056
Ran for 141550 frames Cell Events: 6630 mean_circ: 0.946066
Ran for 142050 frames Cell Events: 6664 mean_circ: 0.946096
Ran for 143050 frames Cell Events: 6706 mean_circ: 0.94608
Ran for 144550 frames Cell Events: 6790 mean_circ: 0.94605
Ran for 145050 frames Cell Events: 6816 mean_circ: 0.946074
Ran for 147050 frames Cell Events: 6931 mean_circ: 0.946102
Ran for 147550 frames Cell Events: 6952 mean_circ: 0.946136
Ran for 148050 frames Cell Events: 6983 mean_circ: 0.946098
Ran for 150050 frames Cell Events: 7109 mean_circ: 0.946071
Ran for 150550 frames Cell Events: 7137 mean_circ: 0.946059
Ran for 153050 frames Cell Events: 7262 mean_circ: 0.946071
Ran for 154050 frames Cell Events: 7317 mean_circ: 0.946032
Ran for 156050 frames Cell Events: 7422 mean_circ: 0.946052
Ran for 156550 frames Cell Events: 7443 mean_circ: 0.946056
Ran for 157550 frames Cell Events: 7496 mean_circ: 0.946038
Ran for 158050 frames Cell Events: 7519 mean_circ: 0.946006
Ran for 159050 frames Cell Events: 7579 mean_circ: 0.94607
Ran for 159550 frames Cell Events: 7608 mean_circ: 0.946066
Ran for 160050 frames Cell Events: 7635 mean_circ: 0.946099
Ran for 162550 frames Cell Events: 7778 mean_circ: 0.946086
Ran for 166050 frames Cell Events: 7971 mean_circ: 0.946145
Ran for 166550 frames Cell Events: 7989 mean_circ: 0.946154
Ran for 167550 frames Cell Events: 8038 mean_circ: 0.946183
Ran for 168550 frames Cell Events: 8097 mean_circ: 0.946192
Ran for 169050 frames Cell Events: 8122 mean_circ: 0.946229
Ran for 171050 frames Cell Events: 8202 mean_circ: 0.946259
Ran for 172050 frames Cell Events: 8259 mean_circ: 0.946261
Ran for 173550 frames Cell Events: 8346 mean_circ: 0.946334
Ran for 177050 frames Cell Events: 8536 mean_circ: 0.946324
Ran for 179550 frames Cell Events: 8638 mean_circ: 0.946266
Ran for 180550 frames Cell Events: 8694 mean_circ: 0.946277
Ran for 181050 frames Cell Events: 8718 mean_circ: 0.946273
Ran for 183550 frames Cell Events: 8862 mean_circ: 0.946242
Ran for 184550 frames Cell Events: 8921 mean_circ: 0.946326
Ran for 185050 frames Cell Events: 8942 mean_circ: 0.946312
Ran for 188050 frames Cell Events: 9091 mean_circ: 0.946315
Ran for 190550 frames Cell Events: 9217 mean_circ: 0.946243
Ran for 191050 frames Cell Events: 9233 mean_circ: 0.946241
Ran for 192050 frames Cell Events: 9287 mean_circ: 0.946238
Ran for 192550 frames Cell Events: 9308 mean_circ: 0.946242
Ran for 195050 frames Cell Events: 9447 mean_circ: 0.946308
Ran for 197050 frames Cell Events: 9553 mean_circ: 0.946262
Ran for 199550 frames Cell Events: 9668 mean_circ: 0.946394
Ran for 200550 frames Cell Events: 9728 mean_circ: 0.946377
Ran for 201050 frames Cell Events: 9758 mean_circ: 0.946367
Ran for 203050 frames Cell Events: 9874 mean_circ: 0.946356



End this measurment.
because stop condition (time/nr of cells) was met

Stopping time: 1.74982e+009
****************************************
Measurement finished

Mean Circularitiy: 0.946369
Mean Area: 589.858
Mean FL-1 intensity: 3261.99
Mean FL-2 intensity: 3529.58
Mean FL-3 intensity: 0

frames processed: 198408
Total Time: 102839ms
Number of Runs (new bg img) 0
Frames collected: 205542
Skipped frames: 15008
Rescued Frames: 340935
Samples DAQ: 51386702
Samples written: 102770824
Samples per Channel: 51385412
FPS: 1998.67

Samples per second: 499667
Expected samples/s: 500000

Events: 10001
Events per second: 97.2488
jumped back (PMT): 3532
#Frames read, frequency
# 0 frames :333929
# 1 frames :58541
# 2 frames :3108
# 3 frames :1426
# 4 frames :929
# 5 frames :586
# 6 frames :848
# 7 frames :1209
# 8 frames :1012
# 9 frames :784
# 10 frames :697
# 11 frames :663
# 12 frames :508
# 13 frames :368
# 14 frames :339
# 15 frames :256
# 16 frames :221
# 17 frames :249
# 18 frames :208
# 19 frames :175
# 20 frames :193
# 21 frames :154
# 22 frames :136
# 23 frames :136
# 24 frames :114
# 25 frames :86
# 26 frames :75
# 27 frames :84
# 28 frames :53
# 29 frames :73
# 30 frames :52
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# 32 frames :36
# 33 frames :42
# 34 frames :47
# 35 frames :36
# 36 frames :25
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# 55 frames :5
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# 60 frames :6
# 61 frames :2
# 62 frames :5
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# 67 frames :3
# 69 frames :1
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# 99 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.5951
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0