Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_12.33.39 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "b37459d42c39753514501d100f0ef26a",
"name": "M1_20170604_1433_dsRNA_E1_faster_aft_clogging.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "10:34:04"
}
}
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.6692
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.75021e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 50 frames Cell Events: 0 mean_circ: 0.944625
Ran for 1050 frames Cell Events: 29 mean_circ: 0.935089
Ran for 1550 frames Cell Events: 47 mean_circ: 0.930842
Ran for 2050 frames Cell Events: 70 mean_circ: 0.930858
Ran for 2550 frames Cell Events: 91 mean_circ: 0.93688
Ran for 5550 frames Cell Events: 209 mean_circ: 0.941861
Ran for 8050 frames Cell Events: 312 mean_circ: 0.940547
Ran for 8550 frames Cell Events: 332 mean_circ: 0.94084
Ran for 9550 frames Cell Events: 375 mean_circ: 0.94179
Ran for 11550 frames Cell Events: 461 mean_circ: 0.942125
Ran for 12050 frames Cell Events: 485 mean_circ: 0.942125
Ran for 12550 frames Cell Events: 509 mean_circ: 0.941698
Ran for 13550 frames Cell Events: 566 mean_circ: 0.942593
Ran for 18050 frames Cell Events: 800 mean_circ: 0.942188
Ran for 18550 frames Cell Events: 823 mean_circ: 0.942187
Ran for 19550 frames Cell Events: 877 mean_circ: 0.942247
Ran for 20050 frames Cell Events: 902 mean_circ: 0.942328
Ran for 20550 frames Cell Events: 921 mean_circ: 0.942201
Ran for 21050 frames Cell Events: 944 mean_circ: 0.942045
Ran for 22050 frames Cell Events: 1000 mean_circ: 0.941968
Ran for 22550 frames Cell Events: 1028 mean_circ: 0.941985
Ran for 23050 frames Cell Events: 1058 mean_circ: 0.941771
Ran for 23550 frames Cell Events: 1072 mean_circ: 0.941613
Ran for 25050 frames Cell Events: 1137 mean_circ: 0.941171
Ran for 26550 frames Cell Events: 1212 mean_circ: 0.941613
Ran for 27550 frames Cell Events: 1265 mean_circ: 0.941713
Ran for 30050 frames Cell Events: 1407 mean_circ: 0.941673
Ran for 32050 frames Cell Events: 1505 mean_circ: 0.941326
Ran for 33550 frames Cell Events: 1559 mean_circ: 0.941107
Ran for 36050 frames Cell Events: 1657 mean_circ: 0.94074
Ran for 38050 frames Cell Events: 1756 mean_circ: 0.940832
Ran for 40050 frames Cell Events: 1828 mean_circ: 0.940745
Ran for 41050 frames Cell Events: 1871 mean_circ: 0.94061
Ran for 44050 frames Cell Events: 2043 mean_circ: 0.940548
Ran for 45550 frames Cell Events: 2131 mean_circ: 0.940784
Ran for 46050 frames Cell Events: 2150 mean_circ: 0.940723
Ran for 49050 frames Cell Events: 2335 mean_circ: 0.940466
Ran for 49550 frames Cell Events: 2365 mean_circ: 0.94034
Ran for 51550 frames Cell Events: 2469 mean_circ: 0.940711
Ran for 52050 frames Cell Events: 2484 mean_circ: 0.940722
Ran for 53050 frames Cell Events: 2520 mean_circ: 0.940753
Ran for 53550 frames Cell Events: 2543 mean_circ: 0.940836
Ran for 54550 frames Cell Events: 2595 mean_circ: 0.940902
Ran for 55550 frames Cell Events: 2647 mean_circ: 0.940729
Ran for 57550 frames Cell Events: 2743 mean_circ: 0.940401
Ran for 58550 frames Cell Events: 2799 mean_circ: 0.940306
Ran for 59050 frames Cell Events: 2822 mean_circ: 0.940199
Ran for 61550 frames Cell Events: 2944 mean_circ: 0.940267
Ran for 62550 frames Cell Events: 2980 mean_circ: 0.940317
Ran for 63550 frames Cell Events: 3032 mean_circ: 0.940147
Ran for 64550 frames Cell Events: 3088 mean_circ: 0.940087
Ran for 65050 frames Cell Events: 3111 mean_circ: 0.939935
Ran for 65550 frames Cell Events: 3135 mean_circ: 0.939958
Ran for 66550 frames Cell Events: 3187 mean_circ: 0.93993
Ran for 67050 frames Cell Events: 3205 mean_circ: 0.939951
Ran for 68050 frames Cell Events: 3267 mean_circ: 0.939903
Ran for 68550 frames Cell Events: 3287 mean_circ: 0.939836
Ran for 70550 frames Cell Events: 3400 mean_circ: 0.9399
Ran for 71550 frames Cell Events: 3442 mean_circ: 0.939793
Ran for 72550 frames Cell Events: 3484 mean_circ: 0.939765
Ran for 73050 frames Cell Events: 3508 mean_circ: 0.939791
Ran for 73550 frames Cell Events: 3525 mean_circ: 0.939778
Ran for 75050 frames Cell Events: 3606 mean_circ: 0.939691
Ran for 76550 frames Cell Events: 3686 mean_circ: 0.939469
Ran for 77550 frames Cell Events: 3740 mean_circ: 0.939291
Ran for 79550 frames Cell Events: 3855 mean_circ: 0.939206
Ran for 81550 frames Cell Events: 3959 mean_circ: 0.93919
Ran for 83050 frames Cell Events: 4024 mean_circ: 0.939222
Ran for 83550 frames Cell Events: 4037 mean_circ: 0.939254
Ran for 84550 frames Cell Events: 4090 mean_circ: 0.939174
Ran for 85050 frames Cell Events: 4130 mean_circ: 0.939177
Ran for 87050 frames Cell Events: 4233 mean_circ: 0.939173
Ran for 91050 frames Cell Events: 4457 mean_circ: 0.938928
Ran for 91550 frames Cell Events: 4484 mean_circ: 0.938966
Ran for 93050 frames Cell Events: 4569 mean_circ: 0.938855
Ran for 93550 frames Cell Events: 4593 mean_circ: 0.93893
Ran for 94050 frames Cell Events: 4622 mean_circ: 0.938916
Ran for 94550 frames Cell Events: 4652 mean_circ: 0.938926
Ran for 95050 frames Cell Events: 4671 mean_circ: 0.938925
Ran for 96550 frames Cell Events: 4765 mean_circ: 0.938976
Ran for 97050 frames Cell Events: 4790 mean_circ: 0.939
Ran for 102050 frames Cell Events: 5081 mean_circ: 0.938764
Ran for 103550 frames Cell Events: 5155 mean_circ: 0.938659
Ran for 105050 frames Cell Events: 5256 mean_circ: 0.938616
Ran for 105550 frames Cell Events: 5286 mean_circ: 0.938501
Ran for 108050 frames Cell Events: 5414 mean_circ: 0.938511
Ran for 111050 frames Cell Events: 5591 mean_circ: 0.938415
Ran for 113050 frames Cell Events: 5688 mean_circ: 0.938399
Ran for 113550 frames Cell Events: 5711 mean_circ: 0.938481
Ran for 114050 frames Cell Events: 5733 mean_circ: 0.938472
Ran for 114550 frames Cell Events: 5756 mean_circ: 0.938505
Ran for 115050 frames Cell Events: 5777 mean_circ: 0.938503
Ran for 116050 frames Cell Events: 5835 mean_circ: 0.938529
Ran for 117550 frames Cell Events: 5911 mean_circ: 0.938433
Ran for 119050 frames Cell Events: 6010 mean_circ: 0.938382
Ran for 119550 frames Cell Events: 6038 mean_circ: 0.938426
Ran for 120550 frames Cell Events: 6095 mean_circ: 0.938431
Ran for 121550 frames Cell Events: 6149 mean_circ: 0.938468
Ran for 122050 frames Cell Events: 6176 mean_circ: 0.938452
Ran for 123550 frames Cell Events: 6267 mean_circ: 0.938488
Ran for 124550 frames Cell Events: 6328 mean_circ: 0.938495
Ran for 125050 frames Cell Events: 6358 mean_circ: 0.938413
Ran for 126050 frames Cell Events: 6413 mean_circ: 0.938367
Ran for 127550 frames Cell Events: 6493 mean_circ: 0.938389
Ran for 131550 frames Cell Events: 6753 mean_circ: 0.938377
Ran for 132050 frames Cell Events: 6777 mean_circ: 0.938393
Ran for 133050 frames Cell Events: 6829 mean_circ: 0.938401
Ran for 134550 frames Cell Events: 6912 mean_circ: 0.938392
Ran for 137550 frames Cell Events: 7100 mean_circ: 0.938344
Ran for 138050 frames Cell Events: 7125 mean_circ: 0.938325
Ran for 139050 frames Cell Events: 7169 mean_circ: 0.938312
Ran for 140550 frames Cell Events: 7234 mean_circ: 0.938331
Ran for 144050 frames Cell Events: 7434 mean_circ: 0.938343
Ran for 146050 frames Cell Events: 7564 mean_circ: 0.938314
Ran for 146550 frames Cell Events: 7596 mean_circ: 0.938319
Ran for 147550 frames Cell Events: 7636 mean_circ: 0.938284
Ran for 150050 frames Cell Events: 7794 mean_circ: 0.938147
Ran for 151550 frames Cell Events: 7861 mean_circ: 0.938084
Ran for 153050 frames Cell Events: 7947 mean_circ: 0.938038
Ran for 155550 frames Cell Events: 8101 mean_circ: 0.937904
Ran for 156550 frames Cell Events: 8149 mean_circ: 0.937898
Ran for 159550 frames Cell Events: 8330 mean_circ: 0.937933
Ran for 161050 frames Cell Events: 8424 mean_circ: 0.937887
Ran for 163550 frames Cell Events: 8581 mean_circ: 0.937778
Ran for 165550 frames Cell Events: 8703 mean_circ: 0.937775
Ran for 166050 frames Cell Events: 8738 mean_circ: 0.937701
Ran for 169050 frames Cell Events: 8914 mean_circ: 0.937752
Ran for 171550 frames Cell Events: 9055 mean_circ: 0.937798
Ran for 172550 frames Cell Events: 9117 mean_circ: 0.937803
Ran for 176050 frames Cell Events: 9290 mean_circ: 0.937746
Ran for 176550 frames Cell Events: 9323 mean_circ: 0.937727
Ran for 177550 frames Cell Events: 9354 mean_circ: 0.937721
Ran for 178050 frames Cell Events: 9383 mean_circ: 0.937702
Ran for 179550 frames Cell Events: 9470 mean_circ: 0.937659
Ran for 181550 frames Cell Events: 9574 mean_circ: 0.937604
Ran for 184050 frames Cell Events: 9730 mean_circ: 0.937636
Ran for 186050 frames Cell Events: 9881 mean_circ: 0.937566
Ran for 187550 frames Cell Events: 9973 mean_circ: 0.937492



End this measurment.
because stop condition (time/nr of cells) was met

Stopping time: 1.7503e+009
****************************************
Measurement finished

Mean Circularitiy: 0.937476
Mean Area: 599.78
Mean FL-1 intensity: 3131.85
Mean FL-2 intensity: 3774.21
Mean FL-3 intensity: 0

frames processed: 178293
Total Time: 94051.1ms
Number of Runs (new bg img) 0
Frames collected: 187966
Skipped frames: 24681
Rescued Frames: 471768
Samples DAQ: 46994879
Samples written: 93982758
Samples per Channel: 46991379
FPS: 1998.55

Samples per second: 499636
Expected samples/s: 500000

Events: 10005
Events per second: 106.378
jumped back (PMT): 56
#Frames read, frequency
# 0 frames :296005
# 1 frames :45774
# 2 frames :2273
# 3 frames :1292
# 4 frames :731
# 5 frames :500
# 6 frames :563
# 7 frames :973
# 8 frames :863
# 9 frames :740
# 10 frames :594
# 11 frames :563
# 12 frames :493
# 13 frames :360
# 14 frames :299
# 15 frames :247
# 16 frames :192
# 17 frames :201
# 18 frames :176
# 19 frames :167
# 20 frames :165
# 21 frames :168
# 22 frames :153
# 23 frames :140
# 24 frames :113
# 25 frames :104
# 26 frames :66
# 27 frames :62
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M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.6692
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0