Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_12.34.40 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "efe99ba10d8007ea568032551bc0b187",
"name": "M1_20170604_1444_dsRNA_G1_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "10:35:00"
}
}
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.8059
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.75091e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 50 frames Cell Events: 0 mean_circ: 0.947623
Ran for 1050 frames Cell Events: 54 mean_circ: 0.936796
Ran for 3550 frames Cell Events: 250 mean_circ: 0.935466
Ran for 6550 frames Cell Events: 462 mean_circ: 0.935174
Ran for 7550 frames Cell Events: 528 mean_circ: 0.935669
Ran for 9050 frames Cell Events: 589 mean_circ: 0.936389
Ran for 9550 frames Cell Events: 613 mean_circ: 0.936781
Ran for 10050 frames Cell Events: 637 mean_circ: 0.936912
Ran for 10550 frames Cell Events: 661 mean_circ: 0.93644
Ran for 11050 frames Cell Events: 682 mean_circ: 0.936589
Ran for 12550 frames Cell Events: 780 mean_circ: 0.936539
Ran for 13550 frames Cell Events: 836 mean_circ: 0.93719
Ran for 15050 frames Cell Events: 879 mean_circ: 0.937297
Ran for 16050 frames Cell Events: 939 mean_circ: 0.936873
Ran for 16550 frames Cell Events: 971 mean_circ: 0.936568
Ran for 17050 frames Cell Events: 1007 mean_circ: 0.936624
Ran for 17550 frames Cell Events: 1030 mean_circ: 0.936624
Ran for 18050 frames Cell Events: 1061 mean_circ: 0.936872
Ran for 19050 frames Cell Events: 1113 mean_circ: 0.93664
Ran for 20550 frames Cell Events: 1202 mean_circ: 0.93714
Ran for 21050 frames Cell Events: 1230 mean_circ: 0.937168
Ran for 22050 frames Cell Events: 1299 mean_circ: 0.936764
Ran for 22550 frames Cell Events: 1323 mean_circ: 0.936957
Ran for 23550 frames Cell Events: 1389 mean_circ: 0.936909
Ran for 25050 frames Cell Events: 1470 mean_circ: 0.936974
Ran for 31050 frames Cell Events: 1804 mean_circ: 0.936786
Ran for 31550 frames Cell Events: 1843 mean_circ: 0.936711
Ran for 33550 frames Cell Events: 1967 mean_circ: 0.936515
Ran for 34050 frames Cell Events: 1986 mean_circ: 0.936669
Ran for 37550 frames Cell Events: 2233 mean_circ: 0.936439
Ran for 39050 frames Cell Events: 2312 mean_circ: 0.936394
Ran for 42050 frames Cell Events: 2490 mean_circ: 0.936407
Ran for 45050 frames Cell Events: 2692 mean_circ: 0.936231
Ran for 46050 frames Cell Events: 2757 mean_circ: 0.936133
Ran for 46550 frames Cell Events: 2775 mean_circ: 0.936059
Ran for 47050 frames Cell Events: 2813 mean_circ: 0.936082
Ran for 48050 frames Cell Events: 2883 mean_circ: 0.936166
Ran for 50050 frames Cell Events: 2993 mean_circ: 0.936229
Ran for 51050 frames Cell Events: 3073 mean_circ: 0.936329
Ran for 52050 frames Cell Events: 3150 mean_circ: 0.936497
Ran for 52550 frames Cell Events: 3182 mean_circ: 0.936609
Ran for 54050 frames Cell Events: 3257 mean_circ: 0.936585
Ran for 54550 frames Cell Events: 3281 mean_circ: 0.936609
Ran for 55050 frames Cell Events: 3308 mean_circ: 0.936616
Ran for 55550 frames Cell Events: 3321 mean_circ: 0.936653
Ran for 61550 frames Cell Events: 3773 mean_circ: 0.936871
Ran for 62050 frames Cell Events: 3790 mean_circ: 0.936918
Ran for 63050 frames Cell Events: 3849 mean_circ: 0.936951
Ran for 64550 frames Cell Events: 3955 mean_circ: 0.936909
Ran for 65550 frames Cell Events: 4040 mean_circ: 0.936894
Ran for 69050 frames Cell Events: 4302 mean_circ: 0.936965
Ran for 70050 frames Cell Events: 4386 mean_circ: 0.936984
Ran for 70550 frames Cell Events: 4438 mean_circ: 0.936984
Ran for 78050 frames Cell Events: 5015 mean_circ: 0.937077
Ran for 78550 frames Cell Events: 5045 mean_circ: 0.937069
Ran for 79550 frames Cell Events: 5105 mean_circ: 0.937063
Ran for 81550 frames Cell Events: 5233 mean_circ: 0.93716
Ran for 84550 frames Cell Events: 5395 mean_circ: 0.936841
Ran for 85050 frames Cell Events: 5416 mean_circ: 0.936863
Ran for 86550 frames Cell Events: 5505 mean_circ: 0.936904
Ran for 87050 frames Cell Events: 5540 mean_circ: 0.936904
Ran for 87550 frames Cell Events: 5575 mean_circ: 0.936893
Ran for 88050 frames Cell Events: 5598 mean_circ: 0.936899
Ran for 90050 frames Cell Events: 5729 mean_circ: 0.936968
Ran for 93550 frames Cell Events: 5976 mean_circ: 0.936974
Ran for 95550 frames Cell Events: 6117 mean_circ: 0.936986
Ran for 96050 frames Cell Events: 6161 mean_circ: 0.937063
Ran for 96550 frames Cell Events: 6195 mean_circ: 0.93706
Ran for 104550 frames Cell Events: 6817 mean_circ: 0.936935
Ran for 105550 frames Cell Events: 6893 mean_circ: 0.936728
Ran for 109050 frames Cell Events: 7146 mean_circ: 0.936656
Ran for 111050 frames Cell Events: 7267 mean_circ: 0.936605
Ran for 112050 frames Cell Events: 7315 mean_circ: 0.936607
Ran for 116550 frames Cell Events: 7598 mean_circ: 0.936545
Ran for 117050 frames Cell Events: 7629 mean_circ: 0.936545
Ran for 117550 frames Cell Events: 7657 mean_circ: 0.936517
Ran for 118050 frames Cell Events: 7680 mean_circ: 0.936507
Ran for 118550 frames Cell Events: 7708 mean_circ: 0.936471
Ran for 119050 frames Cell Events: 7723 mean_circ: 0.936396
Ran for 120050 frames Cell Events: 7766 mean_circ: 0.936359
Ran for 122550 frames Cell Events: 7905 mean_circ: 0.936307
Ran for 123050 frames Cell Events: 7945 mean_circ: 0.936324



End this measurment..



Stopping time: 1.75097e+009
****************************************
Measurement finished

Mean Circularitiy: 0.936344
Mean Area: 628.61
Mean FL-1 intensity: 3544.53
Mean FL-2 intensity: 4528.91
Mean FL-3 intensity: 0

frames processed: 117098
Total Time: 62691.9ms
Number of Runs (new bg img) 0
Frames collected: 125241
Skipped frames: 34706
Rescued Frames: 637684
Samples DAQ: 31317766
Samples written: 62620152
Samples per Channel: 31310076
FPS: 1997.72

Samples per second: 499428
Expected samples/s: 500000

Events: 8082
Events per second: 128.916
jumped back (PMT): 29
#Frames read, frequency
# 0 frames :180785
# 1 frames :24767
# 2 frames :1571
# 3 frames :711
# 4 frames :484
# 5 frames :278
# 6 frames :611
# 7 frames :702
# 8 frames :611
# 9 frames :483
# 10 frames :424
# 11 frames :342
# 12 frames :277
# 13 frames :208
# 14 frames :173
# 15 frames :161
# 16 frames :157
# 17 frames :158
# 18 frames :126
# 19 frames :141
# 20 frames :110
# 21 frames :107
# 22 frames :80
# 23 frames :79
# 24 frames :75
# 25 frames :71
# 26 frames :67
# 27 frames :55
# 28 frames :56
# 29 frames :48
# 30 frames :52
# 31 frames :39
# 32 frames :44
# 33 frames :29
# 34 frames :33
# 35 frames :28
# 36 frames :28
# 37 frames :24
# 38 frames :22
# 39 frames :20
# 40 frames :33
# 41 frames :18
# 42 frames :13
# 43 frames :19
# 44 frames :19
# 45 frames :15
# 46 frames :16
# 47 frames :17
# 48 frames :17
# 49 frames :14
# 50 frames :8
# 51 frames :9
# 52 frames :7
# 53 frames :5
# 54 frames :5
# 55 frames :10
# 56 frames :3
# 57 frames :6
# 58 frames :4
# 59 frames :3
# 60 frames :6
# 61 frames :3
# 62 frames :4
# 63 frames :5
# 64 frames :3
# 65 frames :2
# 66 frames :3
# 67 frames :3
# 68 frames :1
# 70 frames :2
# 71 frames :1
# 73 frames :1
# 74 frames :1
# 75 frames :1
# 76 frames :1
# 77 frames :1
# 78 frames :3
# 82 frames :2
# 83 frames :1
# 86 frames :1
# 87 frames :1
# 88 frames :1
# 89 frames :1
# 96 frames :1
# 97 frames :1
# 98 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.8059
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0