Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_12.35.07 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "46a3d0f4a5f9ec7e5340db373356ea19",
"name": "M1_20170604_1454_dsRNA_F3_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "10:35:27"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8107 (max 8108) in '8107'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.9275
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.75149e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 39 mean_circ: 0.948534
Ran for 1550 frames Cell Events: 60 mean_circ: 0.949917
Ran for 2050 frames Cell Events: 68 mean_circ: 0.949725
Ran for 3550 frames Cell Events: 113 mean_circ: 0.952336
Ran for 7550 frames Cell Events: 269 mean_circ: 0.953408
Ran for 9050 frames Cell Events: 318 mean_circ: 0.953233
Ran for 9550 frames Cell Events: 335 mean_circ: 0.953603
Ran for 10050 frames Cell Events: 356 mean_circ: 0.953603
Ran for 11050 frames Cell Events: 394 mean_circ: 0.953278
Ran for 13050 frames Cell Events: 470 mean_circ: 0.952882
Ran for 13550 frames Cell Events: 496 mean_circ: 0.952747
Ran for 14050 frames Cell Events: 530 mean_circ: 0.952506
Ran for 14550 frames Cell Events: 555 mean_circ: 0.952728
Ran for 15050 frames Cell Events: 583 mean_circ: 0.952728
Ran for 15550 frames Cell Events: 607 mean_circ: 0.952542
Ran for 16050 frames Cell Events: 631 mean_circ: 0.952587
Ran for 16550 frames Cell Events: 658 mean_circ: 0.952608
Ran for 17050 frames Cell Events: 683 mean_circ: 0.952626
Ran for 17550 frames Cell Events: 699 mean_circ: 0.952866
Ran for 19050 frames Cell Events: 763 mean_circ: 0.952792
Ran for 19550 frames Cell Events: 788 mean_circ: 0.952789
Ran for 20050 frames Cell Events: 809 mean_circ: 0.953086
Ran for 20550 frames Cell Events: 829 mean_circ: 0.953255
Ran for 22550 frames Cell Events: 916 mean_circ: 0.952837
Ran for 23050 frames Cell Events: 939 mean_circ: 0.952916
Ran for 24550 frames Cell Events: 1015 mean_circ: 0.953062
Ran for 25050 frames Cell Events: 1036 mean_circ: 0.952926
Ran for 25550 frames Cell Events: 1051 mean_circ: 0.952779
Ran for 26550 frames Cell Events: 1088 mean_circ: 0.952773
Ran for 27050 frames Cell Events: 1110 mean_circ: 0.952675
Ran for 28550 frames Cell Events: 1155 mean_circ: 0.952688
Ran for 29550 frames Cell Events: 1189 mean_circ: 0.952751
Ran for 30050 frames Cell Events: 1209 mean_circ: 0.95284
Ran for 32050 frames Cell Events: 1292 mean_circ: 0.952919
Ran for 34550 frames Cell Events: 1405 mean_circ: 0.952795
Ran for 35050 frames Cell Events: 1438 mean_circ: 0.952776
Ran for 35550 frames Cell Events: 1470 mean_circ: 0.95268
Ran for 36050 frames Cell Events: 1482 mean_circ: 0.952706
Ran for 37050 frames Cell Events: 1521 mean_circ: 0.952699
Ran for 38050 frames Cell Events: 1569 mean_circ: 0.952661
Ran for 38550 frames Cell Events: 1589 mean_circ: 0.952559
Ran for 39550 frames Cell Events: 1631 mean_circ: 0.952505
Ran for 40050 frames Cell Events: 1654 mean_circ: 0.952498
Ran for 40550 frames Cell Events: 1669 mean_circ: 0.952501
Ran for 41550 frames Cell Events: 1707 mean_circ: 0.952599
Ran for 42550 frames Cell Events: 1760 mean_circ: 0.952711
Ran for 43550 frames Cell Events: 1798 mean_circ: 0.952793
Ran for 44050 frames Cell Events: 1818 mean_circ: 0.952805
Ran for 45050 frames Cell Events: 1837 mean_circ: 0.952823
Ran for 46050 frames Cell Events: 1884 mean_circ: 0.952717
Ran for 46550 frames Cell Events: 1906 mean_circ: 0.952538
Ran for 47050 frames Cell Events: 1930 mean_circ: 0.952551
Ran for 47550 frames Cell Events: 1940 mean_circ: 0.952378
Ran for 49050 frames Cell Events: 1997 mean_circ: 0.952215
Ran for 49550 frames Cell Events: 2022 mean_circ: 0.952095
Ran for 50550 frames Cell Events: 2058 mean_circ: 0.952015
Ran for 51550 frames Cell Events: 2103 mean_circ: 0.951973
Ran for 52050 frames Cell Events: 2121 mean_circ: 0.952061
Ran for 52550 frames Cell Events: 2148 mean_circ: 0.952097
Ran for 53550 frames Cell Events: 2185 mean_circ: 0.951776
Ran for 54050 frames Cell Events: 2201 mean_circ: 0.95176
Ran for 56050 frames Cell Events: 2286 mean_circ: 0.951828
Ran for 56550 frames Cell Events: 2299 mean_circ: 0.951913
Ran for 57050 frames Cell Events: 2319 mean_circ: 0.951901
Ran for 57550 frames Cell Events: 2347 mean_circ: 0.951977
Ran for 58050 frames Cell Events: 2368 mean_circ: 0.951785
Ran for 58550 frames Cell Events: 2393 mean_circ: 0.951701
Ran for 61050 frames Cell Events: 2516 mean_circ: 0.951783
Ran for 62050 frames Cell Events: 2577 mean_circ: 0.95169
Ran for 62550 frames Cell Events: 2593 mean_circ: 0.951651
Ran for 63050 frames Cell Events: 2609 mean_circ: 0.951667
Ran for 64550 frames Cell Events: 2651 mean_circ: 0.951692
Ran for 66050 frames Cell Events: 2723 mean_circ: 0.951681
Ran for 66550 frames Cell Events: 2752 mean_circ: 0.951705
Ran for 67050 frames Cell Events: 2767 mean_circ: 0.951729
Ran for 68550 frames Cell Events: 2838 mean_circ: 0.951636
Ran for 69550 frames Cell Events: 2885 mean_circ: 0.951714
Ran for 70550 frames Cell Events: 2920 mean_circ: 0.951715
Ran for 71050 frames Cell Events: 2933 mean_circ: 0.951756
Ran for 71550 frames Cell Events: 2950 mean_circ: 0.951742
Ran for 72050 frames Cell Events: 2972 mean_circ: 0.9517
Ran for 72550 frames Cell Events: 2992 mean_circ: 0.951712
Ran for 73050 frames Cell Events: 3011 mean_circ: 0.951675
Ran for 74050 frames Cell Events: 3052 mean_circ: 0.951635
Ran for 74550 frames Cell Events: 3070 mean_circ: 0.951647
Ran for 75050 frames Cell Events: 3092 mean_circ: 0.951615
Ran for 75550 frames Cell Events: 3111 mean_circ: 0.95159
Ran for 76050 frames Cell Events: 3130 mean_circ: 0.951573
Ran for 77050 frames Cell Events: 3161 mean_circ: 0.951544
Ran for 77550 frames Cell Events: 3186 mean_circ: 0.951576
Ran for 78050 frames Cell Events: 3204 mean_circ: 0.951535
Ran for 78550 frames Cell Events: 3219 mean_circ: 0.951545
Ran for 79050 frames Cell Events: 3230 mean_circ: 0.951532
Ran for 79550 frames Cell Events: 3245 mean_circ: 0.951528
Ran for 80050 frames Cell Events: 3267 mean_circ: 0.951524
Ran for 80550 frames Cell Events: 3286 mean_circ: 0.951443
Ran for 81050 frames Cell Events: 3312 mean_circ: 0.951407
Ran for 82050 frames Cell Events: 3353 mean_circ: 0.951588
Ran for 82550 frames Cell Events: 3383 mean_circ: 0.951588
Ran for 83050 frames Cell Events: 3403 mean_circ: 0.951649
Ran for 84050 frames Cell Events: 3443 mean_circ: 0.951694
Ran for 84550 frames Cell Events: 3456 mean_circ: 0.951699
Ran for 85050 frames Cell Events: 3480 mean_circ: 0.951682
Ran for 85550 frames Cell Events: 3497 mean_circ: 0.951679
Ran for 86050 frames Cell Events: 3524 mean_circ: 0.951748
Ran for 86550 frames Cell Events: 3544 mean_circ: 0.951798
Ran for 87050 frames Cell Events: 3572 mean_circ: 0.951822
Ran for 88550 frames Cell Events: 3642 mean_circ: 0.951806
Ran for 90550 frames Cell Events: 3720 mean_circ: 0.951832
Ran for 91550 frames Cell Events: 3762 mean_circ: 0.951806
Ran for 92550 frames Cell Events: 3801 mean_circ: 0.951802
Ran for 93050 frames Cell Events: 3828 mean_circ: 0.951791
Ran for 93550 frames Cell Events: 3848 mean_circ: 0.951808
Ran for 94050 frames Cell Events: 3868 mean_circ: 0.951834
Ran for 94550 frames Cell Events: 3882 mean_circ: 0.951853
Ran for 95550 frames Cell Events: 3940 mean_circ: 0.951906
Ran for 96550 frames Cell Events: 3977 mean_circ: 0.951925
Ran for 98550 frames Cell Events: 4042 mean_circ: 0.951932
Ran for 99050 frames Cell Events: 4055 mean_circ: 0.95198
Ran for 99550 frames Cell Events: 4083 mean_circ: 0.951944
Ran for 100050 frames Cell Events: 4118 mean_circ: 0.951963
Ran for 100550 frames Cell Events: 4137 mean_circ: 0.952015
Ran for 102050 frames Cell Events: 4211 mean_circ: 0.951741
Ran for 102550 frames Cell Events: 4232 mean_circ: 0.951724
Ran for 103050 frames Cell Events: 4254 mean_circ: 0.951766
Ran for 104050 frames Cell Events: 4294 mean_circ: 0.951691
Ran for 106050 frames Cell Events: 4363 mean_circ: 0.951731
Ran for 107050 frames Cell Events: 4417 mean_circ: 0.951705
Ran for 108550 frames Cell Events: 4471 mean_circ: 0.951657
Ran for 114550 frames Cell Events: 4751 mean_circ: 0.951607
Ran for 115050 frames Cell Events: 4768 mean_circ: 0.95163
Ran for 115550 frames Cell Events: 4792 mean_circ: 0.951639
Ran for 117050 frames Cell Events: 4851 mean_circ: 0.951657
Ran for 118550 frames Cell Events: 4907 mean_circ: 0.951703
Ran for 121050 frames Cell Events: 5010 mean_circ: 0.951763
Ran for 121550 frames Cell Events: 5031 mean_circ: 0.951792
Ran for 122050 frames Cell Events: 5045 mean_circ: 0.951768
Ran for 123050 frames Cell Events: 5083 mean_circ: 0.951808
Ran for 124050 frames Cell Events: 5138 mean_circ: 0.951842
Ran for 124550 frames Cell Events: 5155 mean_circ: 0.951811
Ran for 125550 frames Cell Events: 5196 mean_circ: 0.951797
Ran for 127050 frames Cell Events: 5248 mean_circ: 0.95177
Ran for 129050 frames Cell Events: 5320 mean_circ: 0.95168
Ran for 132050 frames Cell Events: 5472 mean_circ: 0.951708
Ran for 132550 frames Cell Events: 5491 mean_circ: 0.951674
Ran for 134050 frames Cell Events: 5565 mean_circ: 0.951704
Ran for 134550 frames Cell Events: 5586 mean_circ: 0.951665
Ran for 135550 frames Cell Events: 5629 mean_circ: 0.951703
Ran for 136050 frames Cell Events: 5655 mean_circ: 0.951694
Ran for 137050 frames Cell Events: 5707 mean_circ: 0.951699
Ran for 138550 frames Cell Events: 5793 mean_circ: 0.951698
Ran for 139050 frames Cell Events: 5818 mean_circ: 0.951638
Ran for 139550 frames Cell Events: 5841 mean_circ: 0.951651
Ran for 140050 frames Cell Events: 5864 mean_circ: 0.951656
Ran for 142550 frames Cell Events: 5961 mean_circ: 0.951619
Ran for 144050 frames Cell Events: 6026 mean_circ: 0.951613
Ran for 145050 frames Cell Events: 6070 mean_circ: 0.951555
Ran for 145550 frames Cell Events: 6092 mean_circ: 0.951592
Ran for 146050 frames Cell Events: 6110 mean_circ: 0.951628
Ran for 147550 frames Cell Events: 6168 mean_circ: 0.951643
Ran for 148050 frames Cell Events: 6189 mean_circ: 0.951632
Ran for 148550 frames Cell Events: 6207 mean_circ: 0.951643
Ran for 150550 frames Cell Events: 6322 mean_circ: 0.95161
Ran for 151050 frames Cell Events: 6345 mean_circ: 0.951636
Ran for 152050 frames Cell Events: 6396 mean_circ: 0.951546
Ran for 153050 frames Cell Events: 6438 mean_circ: 0.951562
Ran for 154550 frames Cell Events: 6511 mean_circ: 0.951574
Ran for 155550 frames Cell Events: 6558 mean_circ: 0.951493
Ran for 156550 frames Cell Events: 6609 mean_circ: 0.951522
Ran for 157050 frames Cell Events: 6636 mean_circ: 0.951463
Ran for 157550 frames Cell Events: 6654 mean_circ: 0.951449
Ran for 158050 frames Cell Events: 6679 mean_circ: 0.951454
Ran for 158550 frames Cell Events: 6691 mean_circ: 0.951435
Ran for 159050 frames Cell Events: 6708 mean_circ: 0.951434
Ran for 159550 frames Cell Events: 6727 mean_circ: 0.951422
Ran for 160050 frames Cell Events: 6755 mean_circ: 0.951422
Ran for 161050 frames Cell Events: 6806 mean_circ: 0.951398
Ran for 162050 frames Cell Events: 6854 mean_circ: 0.951334
Ran for 162550 frames Cell Events: 6874 mean_circ: 0.951307
Ran for 163050 frames Cell Events: 6900 mean_circ: 0.951318
Ran for 164050 frames Cell Events: 6942 mean_circ: 0.951335
Ran for 165050 frames Cell Events: 6987 mean_circ: 0.951306
Ran for 165550 frames Cell Events: 7005 mean_circ: 0.951295
Ran for 166550 frames Cell Events: 7043 mean_circ: 0.95129
Ran for 167050 frames Cell Events: 7061 mean_circ: 0.951285
Ran for 169050 frames Cell Events: 7161 mean_circ: 0.951257
Ran for 170050 frames Cell Events: 7202 mean_circ: 0.951278
Ran for 172050 frames Cell Events: 7278 mean_circ: 0.951308
Ran for 173050 frames Cell Events: 7316 mean_circ: 0.951291
Ran for 175050 frames Cell Events: 7402 mean_circ: 0.951225
Ran for 177050 frames Cell Events: 7491 mean_circ: 0.951301
Ran for 179550 frames Cell Events: 7590 mean_circ: 0.951224
Ran for 181050 frames Cell Events: 7652 mean_circ: 0.951177
Ran for 181550 frames Cell Events: 7670 mean_circ: 0.95119
Ran for 183550 frames Cell Events: 7750 mean_circ: 0.951182
Ran for 184050 frames Cell Events: 7771 mean_circ: 0.95123
Ran for 184550 frames Cell Events: 7787 mean_circ: 0.951247
Ran for 186050 frames Cell Events: 7866 mean_circ: 0.951249
Ran for 186550 frames Cell Events: 7892 mean_circ: 0.951244
Ran for 187050 frames Cell Events: 7936 mean_circ: 0.951223
Ran for 188550 frames Cell Events: 8027 mean_circ: 0.951219
Ran for 189050 frames Cell Events: 8046 mean_circ: 0.951226



End this measurment..



Stopping time: 1.75158e+009
****************************************
Measurement finished

Mean Circularitiy: 0.95123
Mean Area: 568.142
Mean FL-1 intensity: 3162.85
Mean FL-2 intensity: 3568.46
Mean FL-3 intensity: 0

frames processed: 187813
Total Time: 95233.5ms
Number of Runs (new bg img) 0
Frames collected: 190334
Skipped frames: 37227
Rescued Frames: 733408
Samples DAQ: 47583458
Samples written: 95166570
Samples per Channel: 47583285
FPS: 1998.6

Samples per second: 499649
Expected samples/s: 500000

Events: 8108
Events per second: 85.1381
jumped back (PMT): 3795
#Frames read, frequency
# 0 frames :288538
# 1 frames :77315
# 2 frames :3536
# 3 frames :1519
# 4 frames :865
# 5 frames :498
# 6 frames :1275
# 7 frames :1411
# 8 frames :1160
# 9 frames :755
# 10 frames :630
# 11 frames :501
# 12 frames :418
# 13 frames :296
# 14 frames :285
# 15 frames :226
# 16 frames :219
# 17 frames :183
# 18 frames :123
# 19 frames :123
# 20 frames :93
# 21 frames :85
# 22 frames :89
# 23 frames :56
# 24 frames :55
# 25 frames :54
# 26 frames :37
# 27 frames :29
# 28 frames :22
# 29 frames :33
# 30 frames :27
# 31 frames :20
# 32 frames :13
# 33 frames :13
# 34 frames :15
# 35 frames :14
# 36 frames :15
# 37 frames :10
# 38 frames :5
# 39 frames :10
# 40 frames :9
# 41 frames :8
# 42 frames :6
# 43 frames :5
# 44 frames :7
# 45 frames :4
# 46 frames :6
# 47 frames :2
# 48 frames :4
# 49 frames :3
# 50 frames :2
# 51 frames :1
# 52 frames :2
# 53 frames :4
# 54 frames :2
# 56 frames :1
# 57 frames :2
# 58 frames :2
# 59 frames :2
# 62 frames :1
# 64 frames :1
# 65 frames :1
# 67 frames :1
# 68 frames :1
# 74 frames :1
# 91 frames :1
# 94 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.9275
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0