Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_12.36.03 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "2fc77a15aae44d867167f68bf277c5c7",
"name": "M1_20170604_1505_dsRNA_E5_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "10:36:23"
}
}
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.9217
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.75215e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 50 frames Cell Events: 0 mean_circ: 0.940018
Ran for 550 frames Cell Events: 8 mean_circ: 0.952862
Ran for 1550 frames Cell Events: 42 mean_circ: 0.942684
Ran for 2050 frames Cell Events: 69 mean_circ: 0.943061
Ran for 3050 frames Cell Events: 101 mean_circ: 0.939741
Ran for 4550 frames Cell Events: 145 mean_circ: 0.941537
Ran for 5050 frames Cell Events: 164 mean_circ: 0.94165
Ran for 6050 frames Cell Events: 189 mean_circ: 0.941763
Ran for 7050 frames Cell Events: 230 mean_circ: 0.942393
Ran for 8550 frames Cell Events: 284 mean_circ: 0.944683
Ran for 9550 frames Cell Events: 321 mean_circ: 0.943618
Ran for 10050 frames Cell Events: 336 mean_circ: 0.943899
Ran for 10550 frames Cell Events: 356 mean_circ: 0.943468
Ran for 11050 frames Cell Events: 371 mean_circ: 0.943347
Ran for 11550 frames Cell Events: 390 mean_circ: 0.942908
Ran for 12550 frames Cell Events: 426 mean_circ: 0.942605
Ran for 13050 frames Cell Events: 442 mean_circ: 0.943083
Ran for 13550 frames Cell Events: 455 mean_circ: 0.942119
Ran for 14050 frames Cell Events: 473 mean_circ: 0.94226
Ran for 15050 frames Cell Events: 506 mean_circ: 0.94178
Ran for 15550 frames Cell Events: 525 mean_circ: 0.941934
Ran for 17050 frames Cell Events: 566 mean_circ: 0.941891
Ran for 18550 frames Cell Events: 618 mean_circ: 0.941414
Ran for 19050 frames Cell Events: 637 mean_circ: 0.941227
Ran for 20050 frames Cell Events: 666 mean_circ: 0.940642
Ran for 20550 frames Cell Events: 686 mean_circ: 0.940982
Ran for 21550 frames Cell Events: 721 mean_circ: 0.941067
Ran for 23050 frames Cell Events: 756 mean_circ: 0.941285
Ran for 23550 frames Cell Events: 782 mean_circ: 0.941475
Ran for 24050 frames Cell Events: 790 mean_circ: 0.941539
Ran for 24550 frames Cell Events: 804 mean_circ: 0.941155
Ran for 25050 frames Cell Events: 824 mean_circ: 0.94086
Ran for 25550 frames Cell Events: 839 mean_circ: 0.940932
Ran for 29550 frames Cell Events: 1009 mean_circ: 0.940406
Ran for 30050 frames Cell Events: 1034 mean_circ: 0.940255
Ran for 30550 frames Cell Events: 1048 mean_circ: 0.940366
Ran for 32550 frames Cell Events: 1119 mean_circ: 0.940211
Ran for 33050 frames Cell Events: 1136 mean_circ: 0.9401
Ran for 34050 frames Cell Events: 1179 mean_circ: 0.940065
Ran for 35050 frames Cell Events: 1207 mean_circ: 0.940087
Ran for 36050 frames Cell Events: 1260 mean_circ: 0.939829
Ran for 37050 frames Cell Events: 1308 mean_circ: 0.940402
Ran for 37550 frames Cell Events: 1323 mean_circ: 0.940366
Ran for 38050 frames Cell Events: 1339 mean_circ: 0.940299
Ran for 38550 frames Cell Events: 1354 mean_circ: 0.940459
Ran for 39550 frames Cell Events: 1399 mean_circ: 0.939902
Ran for 41550 frames Cell Events: 1472 mean_circ: 0.940054
Ran for 42050 frames Cell Events: 1491 mean_circ: 0.940194
Ran for 42550 frames Cell Events: 1506 mean_circ: 0.940155
Ran for 44550 frames Cell Events: 1574 mean_circ: 0.940253
Ran for 45550 frames Cell Events: 1614 mean_circ: 0.94038
Ran for 46050 frames Cell Events: 1624 mean_circ: 0.940374
Ran for 46550 frames Cell Events: 1642 mean_circ: 0.940476
Ran for 47050 frames Cell Events: 1658 mean_circ: 0.94043
Ran for 52550 frames Cell Events: 1914 mean_circ: 0.940337
Ran for 54050 frames Cell Events: 1970 mean_circ: 0.940544
Ran for 55050 frames Cell Events: 2001 mean_circ: 0.940537
Ran for 55550 frames Cell Events: 2018 mean_circ: 0.940434
Ran for 56550 frames Cell Events: 2071 mean_circ: 0.940522
Ran for 57550 frames Cell Events: 2104 mean_circ: 0.940137
Ran for 58050 frames Cell Events: 2123 mean_circ: 0.940047
Ran for 59050 frames Cell Events: 2160 mean_circ: 0.940316
Ran for 60050 frames Cell Events: 2195 mean_circ: 0.940498
Ran for 61050 frames Cell Events: 2236 mean_circ: 0.940475
Ran for 62050 frames Cell Events: 2258 mean_circ: 0.940374
Ran for 63550 frames Cell Events: 2321 mean_circ: 0.940225
Ran for 64050 frames Cell Events: 2338 mean_circ: 0.940286
Ran for 64550 frames Cell Events: 2356 mean_circ: 0.940337
Ran for 65050 frames Cell Events: 2371 mean_circ: 0.940344
Ran for 67050 frames Cell Events: 2454 mean_circ: 0.940173
Ran for 67550 frames Cell Events: 2469 mean_circ: 0.940203
Ran for 69050 frames Cell Events: 2518 mean_circ: 0.940163
Ran for 70050 frames Cell Events: 2560 mean_circ: 0.94018
Ran for 71550 frames Cell Events: 2621 mean_circ: 0.940129
Ran for 72550 frames Cell Events: 2667 mean_circ: 0.939798
Ran for 73050 frames Cell Events: 2696 mean_circ: 0.939645
Ran for 74550 frames Cell Events: 2737 mean_circ: 0.939569
Ran for 76050 frames Cell Events: 2791 mean_circ: 0.939702
Ran for 76550 frames Cell Events: 2807 mean_circ: 0.939711
Ran for 77550 frames Cell Events: 2859 mean_circ: 0.939742
Ran for 78550 frames Cell Events: 2894 mean_circ: 0.939669
Ran for 79050 frames Cell Events: 2920 mean_circ: 0.93974
Ran for 79550 frames Cell Events: 2939 mean_circ: 0.939747
Ran for 80050 frames Cell Events: 2953 mean_circ: 0.939768
Ran for 81050 frames Cell Events: 2996 mean_circ: 0.939494
Ran for 81550 frames Cell Events: 3012 mean_circ: 0.939499
Ran for 82050 frames Cell Events: 3030 mean_circ: 0.939486
Ran for 83550 frames Cell Events: 3095 mean_circ: 0.939431
Ran for 84550 frames Cell Events: 3140 mean_circ: 0.939567
Ran for 85550 frames Cell Events: 3194 mean_circ: 0.939699
Ran for 86050 frames Cell Events: 3219 mean_circ: 0.939633
Ran for 90550 frames Cell Events: 3421 mean_circ: 0.939674
Ran for 91050 frames Cell Events: 3436 mean_circ: 0.939706
Ran for 91550 frames Cell Events: 3457 mean_circ: 0.939771
Ran for 92050 frames Cell Events: 3474 mean_circ: 0.939733
Ran for 93050 frames Cell Events: 3517 mean_circ: 0.939775
Ran for 93550 frames Cell Events: 3533 mean_circ: 0.93981
Ran for 96050 frames Cell Events: 3633 mean_circ: 0.939873
Ran for 96550 frames Cell Events: 3652 mean_circ: 0.939805
Ran for 97550 frames Cell Events: 3697 mean_circ: 0.939661
Ran for 98050 frames Cell Events: 3717 mean_circ: 0.939624
Ran for 99050 frames Cell Events: 3762 mean_circ: 0.93963
Ran for 100050 frames Cell Events: 3804 mean_circ: 0.939581
Ran for 100550 frames Cell Events: 3821 mean_circ: 0.939572
Ran for 102050 frames Cell Events: 3864 mean_circ: 0.939524
Ran for 103050 frames Cell Events: 3914 mean_circ: 0.939516
Ran for 104050 frames Cell Events: 3981 mean_circ: 0.939375
Ran for 104550 frames Cell Events: 4001 mean_circ: 0.93936
Ran for 105050 frames Cell Events: 4026 mean_circ: 0.939345
Ran for 106050 frames Cell Events: 4060 mean_circ: 0.939403
Ran for 109050 frames Cell Events: 4184 mean_circ: 0.939392
Ran for 109550 frames Cell Events: 4208 mean_circ: 0.939341
Ran for 110050 frames Cell Events: 4231 mean_circ: 0.939413
Ran for 110550 frames Cell Events: 4244 mean_circ: 0.939355
Ran for 111050 frames Cell Events: 4264 mean_circ: 0.939387
Ran for 111550 frames Cell Events: 4284 mean_circ: 0.939419
Ran for 112050 frames Cell Events: 4304 mean_circ: 0.93942
Ran for 113050 frames Cell Events: 4330 mean_circ: 0.939394
Ran for 113550 frames Cell Events: 4341 mean_circ: 0.939447
Ran for 114550 frames Cell Events: 4399 mean_circ: 0.939476
Ran for 115050 frames Cell Events: 4419 mean_circ: 0.939532
Ran for 115550 frames Cell Events: 4435 mean_circ: 0.939585
Ran for 117050 frames Cell Events: 4495 mean_circ: 0.93956
Ran for 117550 frames Cell Events: 4513 mean_circ: 0.939571
Ran for 118550 frames Cell Events: 4559 mean_circ: 0.939447
Ran for 119050 frames Cell Events: 4577 mean_circ: 0.939445
Ran for 119550 frames Cell Events: 4603 mean_circ: 0.939405
Ran for 120050 frames Cell Events: 4621 mean_circ: 0.939379
Ran for 121050 frames Cell Events: 4668 mean_circ: 0.939407
Ran for 123550 frames Cell Events: 4777 mean_circ: 0.939153
Ran for 124050 frames Cell Events: 4793 mean_circ: 0.93913
Ran for 125050 frames Cell Events: 4822 mean_circ: 0.939147
Ran for 125550 frames Cell Events: 4835 mean_circ: 0.939139
Ran for 127050 frames Cell Events: 4900 mean_circ: 0.938961
Ran for 127550 frames Cell Events: 4919 mean_circ: 0.938936
Ran for 128050 frames Cell Events: 4939 mean_circ: 0.938892
Ran for 128550 frames Cell Events: 4958 mean_circ: 0.938846
Ran for 129550 frames Cell Events: 4996 mean_circ: 0.938793
Ran for 130050 frames Cell Events: 5009 mean_circ: 0.938806
Ran for 131550 frames Cell Events: 5067 mean_circ: 0.938629
Ran for 132050 frames Cell Events: 5092 mean_circ: 0.938641
Ran for 132550 frames Cell Events: 5110 mean_circ: 0.938699
Ran for 135050 frames Cell Events: 5218 mean_circ: 0.938559
Ran for 135550 frames Cell Events: 5231 mean_circ: 0.938547
Ran for 138050 frames Cell Events: 5312 mean_circ: 0.938503
Ran for 138550 frames Cell Events: 5331 mean_circ: 0.938402
Ran for 139550 frames Cell Events: 5389 mean_circ: 0.938391
Ran for 140550 frames Cell Events: 5428 mean_circ: 0.938229
Ran for 142050 frames Cell Events: 5489 mean_circ: 0.93832
Ran for 143050 frames Cell Events: 5519 mean_circ: 0.938281
Ran for 143550 frames Cell Events: 5541 mean_circ: 0.938288
Ran for 144050 frames Cell Events: 5559 mean_circ: 0.9383
Ran for 144550 frames Cell Events: 5575 mean_circ: 0.938279
Ran for 145550 frames Cell Events: 5613 mean_circ: 0.938294
Ran for 146050 frames Cell Events: 5632 mean_circ: 0.938324
Ran for 146550 frames Cell Events: 5659 mean_circ: 0.93828
Ran for 147050 frames Cell Events: 5672 mean_circ: 0.938297
Ran for 147550 frames Cell Events: 5686 mean_circ: 0.938298
Ran for 148050 frames Cell Events: 5707 mean_circ: 0.938249
Ran for 148550 frames Cell Events: 5726 mean_circ: 0.938278
Ran for 149050 frames Cell Events: 5741 mean_circ: 0.938308
Ran for 149550 frames Cell Events: 5755 mean_circ: 0.938338
Ran for 150050 frames Cell Events: 5778 mean_circ: 0.938338
Ran for 151050 frames Cell Events: 5824 mean_circ: 0.938262
Ran for 152050 frames Cell Events: 5861 mean_circ: 0.938248
Ran for 152550 frames Cell Events: 5890 mean_circ: 0.93825
Ran for 153050 frames Cell Events: 5911 mean_circ: 0.938251
Ran for 154050 frames Cell Events: 5952 mean_circ: 0.938348
Ran for 156550 frames Cell Events: 6057 mean_circ: 0.938414
Ran for 158550 frames Cell Events: 6125 mean_circ: 0.938375
Ran for 162050 frames Cell Events: 6265 mean_circ: 0.938308
Ran for 162550 frames Cell Events: 6285 mean_circ: 0.938266
Ran for 163050 frames Cell Events: 6297 mean_circ: 0.938257
Ran for 163550 frames Cell Events: 6324 mean_circ: 0.938292
Ran for 164050 frames Cell Events: 6352 mean_circ: 0.938284
Ran for 165550 frames Cell Events: 6426 mean_circ: 0.93829
Ran for 168050 frames Cell Events: 6519 mean_circ: 0.938218
Ran for 168550 frames Cell Events: 6541 mean_circ: 0.938177
Ran for 170050 frames Cell Events: 6614 mean_circ: 0.938059
Ran for 170550 frames Cell Events: 6623 mean_circ: 0.938027
Ran for 171050 frames Cell Events: 6641 mean_circ: 0.938057
Ran for 172050 frames Cell Events: 6681 mean_circ: 0.938066
Ran for 173550 frames Cell Events: 6746 mean_circ: 0.938038
Ran for 174050 frames Cell Events: 6767 mean_circ: 0.937982
Ran for 175050 frames Cell Events: 6801 mean_circ: 0.937921
Ran for 175550 frames Cell Events: 6816 mean_circ: 0.937929
Ran for 177550 frames Cell Events: 6897 mean_circ: 0.937825
Ran for 178050 frames Cell Events: 6916 mean_circ: 0.93785
Ran for 179050 frames Cell Events: 6947 mean_circ: 0.937844
Ran for 180050 frames Cell Events: 6989 mean_circ: 0.93782
Ran for 180550 frames Cell Events: 7007 mean_circ: 0.937791
Ran for 181050 frames Cell Events: 7021 mean_circ: 0.937822
Ran for 181550 frames Cell Events: 7043 mean_circ: 0.937831
Ran for 182050 frames Cell Events: 7055 mean_circ: 0.937811
Ran for 182550 frames Cell Events: 7079 mean_circ: 0.937819
Ran for 183050 frames Cell Events: 7096 mean_circ: 0.937831
Ran for 184050 frames Cell Events: 7147 mean_circ: 0.93777
Ran for 184550 frames Cell Events: 7166 mean_circ: 0.937751
Ran for 185050 frames Cell Events: 7183 mean_circ: 0.937776
Ran for 185550 frames Cell Events: 7201 mean_circ: 0.937756
Ran for 186050 frames Cell Events: 7214 mean_circ: 0.937755
Ran for 186550 frames Cell Events: 7230 mean_circ: 0.937741
Ran for 188550 frames Cell Events: 7314 mean_circ: 0.937752
Ran for 190050 frames Cell Events: 7393 mean_circ: 0.937728
Ran for 193050 frames Cell Events: 7517 mean_circ: 0.937678
Ran for 193550 frames Cell Events: 7535 mean_circ: 0.937686
Ran for 194050 frames Cell Events: 7541 mean_circ: 0.937698
Ran for 195550 frames Cell Events: 7614 mean_circ: 0.93768
Ran for 196050 frames Cell Events: 7644 mean_circ: 0.93768
Ran for 196550 frames Cell Events: 7662 mean_circ: 0.93768
Ran for 198550 frames Cell Events: 7753 mean_circ: 0.937713
Ran for 199050 frames Cell Events: 7771 mean_circ: 0.937724
Ran for 201050 frames Cell Events: 7867 mean_circ: 0.937728
Ran for 202050 frames Cell Events: 7897 mean_circ: 0.937717
Ran for 204550 frames Cell Events: 7983 mean_circ: 0.937703
Ran for 206050 frames Cell Events: 8056 mean_circ: 0.937754
Ran for 206550 frames Cell Events: 8073 mean_circ: 0.937735



End this measurment..



Stopping time: 1.75225e+009
****************************************
Measurement finished

Mean Circularitiy: 0.937733
Mean Area: 610.771
Mean FL-1 intensity: 2744.59
Mean FL-2 intensity: 3221.87
Mean FL-3 intensity: 0

frames processed: 204206
Total Time: 103560ms
Number of Runs (new bg img) 0
Frames collected: 206975
Skipped frames: 42326
Rescued Frames: 928050
Samples DAQ: 51747526
Samples written: 103487228
Samples per Channel: 51743614
FPS: 1998.61

Samples per second: 499651
Expected samples/s: 500000

Events: 8094
Events per second: 78.1579
jumped back (PMT): 3375
#Frames read, frequency
# 0 frames :320805
# 1 frames :77333
# 2 frames :3371
# 3 frames :1714
# 4 frames :913
# 5 frames :663
# 6 frames :656
# 7 frames :1178
# 8 frames :1065
# 9 frames :851
# 10 frames :740
# 11 frames :680
# 12 frames :596
# 13 frames :410
# 14 frames :303
# 15 frames :248
# 16 frames :201
# 17 frames :204
# 18 frames :167
# 19 frames :145
# 20 frames :143
# 21 frames :149
# 22 frames :129
# 23 frames :111
# 24 frames :80
# 25 frames :62
# 26 frames :63
# 27 frames :47
# 28 frames :53
# 29 frames :39
# 30 frames :37
# 31 frames :31
# 32 frames :29
# 33 frames :23
# 34 frames :22
# 35 frames :21
# 36 frames :16
# 37 frames :22
# 38 frames :11
# 39 frames :13
# 40 frames :15
# 41 frames :8
# 42 frames :8
# 43 frames :8
# 44 frames :13
# 45 frames :9
# 46 frames :9
# 47 frames :8
# 48 frames :4
# 49 frames :3
# 50 frames :5
# 51 frames :2
# 52 frames :1
# 53 frames :3
# 55 frames :2
# 56 frames :2
# 58 frames :2
# 59 frames :4
# 60 frames :3
# 61 frames :2
# 62 frames :2
# 64 frames :1
# 65 frames :4
# 66 frames :3
# 67 frames :2
# 69 frames :3
# 70 frames :1
# 72 frames :1
# 77 frames :1
# 78 frames :1
# 85 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.9217
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0