Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_12.38.04 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "94fde6f2b1bbc0b78812c553fec71121",
"name": "M1_20170604_1530_dsRNA_F2_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "10:38:24"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8062 (max 8063) in '8062'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.0378
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.75365e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 28 mean_circ: 0.971263
Ran for 2050 frames Cell Events: 85 mean_circ: 0.959537
Ran for 2550 frames Cell Events: 110 mean_circ: 0.95484
Ran for 3050 frames Cell Events: 133 mean_circ: 0.954981
Ran for 3550 frames Cell Events: 153 mean_circ: 0.956351
Ran for 4050 frames Cell Events: 181 mean_circ: 0.953907
Ran for 5050 frames Cell Events: 230 mean_circ: 0.955283
Ran for 5550 frames Cell Events: 249 mean_circ: 0.956182
Ran for 6050 frames Cell Events: 274 mean_circ: 0.956283
Ran for 6550 frames Cell Events: 298 mean_circ: 0.956082
Ran for 7050 frames Cell Events: 318 mean_circ: 0.955674
Ran for 10550 frames Cell Events: 468 mean_circ: 0.954022
Ran for 12050 frames Cell Events: 544 mean_circ: 0.953876
Ran for 15050 frames Cell Events: 709 mean_circ: 0.953447
Ran for 17550 frames Cell Events: 844 mean_circ: 0.953705
Ran for 18550 frames Cell Events: 893 mean_circ: 0.953697
Ran for 22050 frames Cell Events: 1044 mean_circ: 0.95356
Ran for 23050 frames Cell Events: 1084 mean_circ: 0.953231
Ran for 25050 frames Cell Events: 1184 mean_circ: 0.953459
Ran for 26050 frames Cell Events: 1237 mean_circ: 0.953412
Ran for 27050 frames Cell Events: 1276 mean_circ: 0.95345
Ran for 31050 frames Cell Events: 1456 mean_circ: 0.953593
Ran for 32050 frames Cell Events: 1503 mean_circ: 0.953663
Ran for 32550 frames Cell Events: 1525 mean_circ: 0.953817
Ran for 33550 frames Cell Events: 1568 mean_circ: 0.953887
Ran for 34550 frames Cell Events: 1608 mean_circ: 0.954026
Ran for 36550 frames Cell Events: 1682 mean_circ: 0.953639
Ran for 37050 frames Cell Events: 1711 mean_circ: 0.953607
Ran for 39050 frames Cell Events: 1803 mean_circ: 0.953597
Ran for 40550 frames Cell Events: 1847 mean_circ: 0.953425
Ran for 42050 frames Cell Events: 1926 mean_circ: 0.953225
Ran for 44550 frames Cell Events: 2002 mean_circ: 0.953132
Ran for 46050 frames Cell Events: 2088 mean_circ: 0.953172
Ran for 46550 frames Cell Events: 2105 mean_circ: 0.952988
Ran for 48050 frames Cell Events: 2166 mean_circ: 0.952748
Ran for 48550 frames Cell Events: 2188 mean_circ: 0.952746
Ran for 49550 frames Cell Events: 2237 mean_circ: 0.952705
Ran for 50050 frames Cell Events: 2271 mean_circ: 0.952705
Ran for 51550 frames Cell Events: 2339 mean_circ: 0.952331
Ran for 52050 frames Cell Events: 2362 mean_circ: 0.952159
Ran for 52550 frames Cell Events: 2377 mean_circ: 0.952167
Ran for 53050 frames Cell Events: 2398 mean_circ: 0.952131
Ran for 53550 frames Cell Events: 2417 mean_circ: 0.952101
Ran for 54550 frames Cell Events: 2447 mean_circ: 0.952138
Ran for 57050 frames Cell Events: 2534 mean_circ: 0.952284
Ran for 58550 frames Cell Events: 2595 mean_circ: 0.952315
Ran for 59050 frames Cell Events: 2606 mean_circ: 0.952362
Ran for 60550 frames Cell Events: 2666 mean_circ: 0.952254
Ran for 62050 frames Cell Events: 2743 mean_circ: 0.95224
Ran for 65550 frames Cell Events: 2894 mean_circ: 0.952099
Ran for 66550 frames Cell Events: 2940 mean_circ: 0.952135
Ran for 67550 frames Cell Events: 2984 mean_circ: 0.952052
Ran for 69550 frames Cell Events: 3055 mean_circ: 0.952061
Ran for 70550 frames Cell Events: 3103 mean_circ: 0.952078
Ran for 71050 frames Cell Events: 3128 mean_circ: 0.9521
Ran for 71550 frames Cell Events: 3150 mean_circ: 0.952102
Ran for 72050 frames Cell Events: 3175 mean_circ: 0.952102
Ran for 72550 frames Cell Events: 3197 mean_circ: 0.952187
Ran for 73550 frames Cell Events: 3242 mean_circ: 0.952142
Ran for 75550 frames Cell Events: 3343 mean_circ: 0.952177
Ran for 76550 frames Cell Events: 3389 mean_circ: 0.952149
Ran for 77050 frames Cell Events: 3420 mean_circ: 0.952237
Ran for 78050 frames Cell Events: 3469 mean_circ: 0.952219
Ran for 78550 frames Cell Events: 3498 mean_circ: 0.95222
Ran for 80550 frames Cell Events: 3588 mean_circ: 0.952126
Ran for 81050 frames Cell Events: 3613 mean_circ: 0.952102
Ran for 82050 frames Cell Events: 3669 mean_circ: 0.952111
Ran for 83550 frames Cell Events: 3737 mean_circ: 0.952201
Ran for 84550 frames Cell Events: 3791 mean_circ: 0.952239
Ran for 85050 frames Cell Events: 3818 mean_circ: 0.952255
Ran for 86550 frames Cell Events: 3900 mean_circ: 0.952334
Ran for 87550 frames Cell Events: 3936 mean_circ: 0.952242
Ran for 88050 frames Cell Events: 3961 mean_circ: 0.952242
Ran for 88550 frames Cell Events: 3975 mean_circ: 0.952244
Ran for 89050 frames Cell Events: 3999 mean_circ: 0.952251
Ran for 89550 frames Cell Events: 4024 mean_circ: 0.952283
Ran for 91050 frames Cell Events: 4089 mean_circ: 0.952274
Ran for 91550 frames Cell Events: 4109 mean_circ: 0.952284
Ran for 92050 frames Cell Events: 4124 mean_circ: 0.952322
Ran for 92550 frames Cell Events: 4147 mean_circ: 0.952321
Ran for 93550 frames Cell Events: 4205 mean_circ: 0.952292
Ran for 94050 frames Cell Events: 4238 mean_circ: 0.952292
Ran for 94550 frames Cell Events: 4269 mean_circ: 0.952326
Ran for 95050 frames Cell Events: 4311 mean_circ: 0.952361
Ran for 100050 frames Cell Events: 4536 mean_circ: 0.952171
Ran for 101550 frames Cell Events: 4613 mean_circ: 0.952065
Ran for 102050 frames Cell Events: 4641 mean_circ: 0.952063
Ran for 102550 frames Cell Events: 4665 mean_circ: 0.952078
Ran for 103050 frames Cell Events: 4691 mean_circ: 0.952002
Ran for 104550 frames Cell Events: 4747 mean_circ: 0.952041
Ran for 105050 frames Cell Events: 4763 mean_circ: 0.952059
Ran for 106550 frames Cell Events: 4826 mean_circ: 0.952063
Ran for 107550 frames Cell Events: 4861 mean_circ: 0.952081
Ran for 109050 frames Cell Events: 4926 mean_circ: 0.952119
Ran for 109550 frames Cell Events: 4951 mean_circ: 0.952171
Ran for 110550 frames Cell Events: 4992 mean_circ: 0.952149
Ran for 114050 frames Cell Events: 5118 mean_circ: 0.952228
Ran for 115550 frames Cell Events: 5200 mean_circ: 0.952194
Ran for 116050 frames Cell Events: 5219 mean_circ: 0.952145
Ran for 117050 frames Cell Events: 5266 mean_circ: 0.952169
Ran for 117550 frames Cell Events: 5298 mean_circ: 0.952198
Ran for 118050 frames Cell Events: 5320 mean_circ: 0.952189
Ran for 119550 frames Cell Events: 5385 mean_circ: 0.952151
Ran for 120050 frames Cell Events: 5412 mean_circ: 0.952138
Ran for 121050 frames Cell Events: 5459 mean_circ: 0.95218
Ran for 122050 frames Cell Events: 5509 mean_circ: 0.95218
Ran for 122550 frames Cell Events: 5537 mean_circ: 0.952207
Ran for 123050 frames Cell Events: 5554 mean_circ: 0.952187
Ran for 123550 frames Cell Events: 5580 mean_circ: 0.952202
Ran for 125050 frames Cell Events: 5661 mean_circ: 0.95225
Ran for 126050 frames Cell Events: 5698 mean_circ: 0.952263
Ran for 127550 frames Cell Events: 5749 mean_circ: 0.952297
Ran for 128550 frames Cell Events: 5798 mean_circ: 0.952325
Ran for 129050 frames Cell Events: 5816 mean_circ: 0.95228
Ran for 129550 frames Cell Events: 5847 mean_circ: 0.952297
Ran for 131050 frames Cell Events: 5919 mean_circ: 0.952293
Ran for 132050 frames Cell Events: 5964 mean_circ: 0.952343
Ran for 133050 frames Cell Events: 5998 mean_circ: 0.952356
Ran for 134050 frames Cell Events: 6045 mean_circ: 0.952336
Ran for 134550 frames Cell Events: 6065 mean_circ: 0.95232
Ran for 135050 frames Cell Events: 6081 mean_circ: 0.952301
Ran for 135550 frames Cell Events: 6107 mean_circ: 0.952301
Ran for 137050 frames Cell Events: 6171 mean_circ: 0.952345
Ran for 140050 frames Cell Events: 6305 mean_circ: 0.952315
Ran for 140550 frames Cell Events: 6336 mean_circ: 0.952308
Ran for 141550 frames Cell Events: 6388 mean_circ: 0.952355
Ran for 142050 frames Cell Events: 6410 mean_circ: 0.952369
Ran for 142550 frames Cell Events: 6440 mean_circ: 0.952317
Ran for 145550 frames Cell Events: 6591 mean_circ: 0.952248
Ran for 146050 frames Cell Events: 6613 mean_circ: 0.952248
Ran for 147050 frames Cell Events: 6669 mean_circ: 0.952168
Ran for 147550 frames Cell Events: 6689 mean_circ: 0.95219
Ran for 148050 frames Cell Events: 6707 mean_circ: 0.952184
Ran for 148550 frames Cell Events: 6733 mean_circ: 0.952185
Ran for 149050 frames Cell Events: 6751 mean_circ: 0.952176
Ran for 150050 frames Cell Events: 6792 mean_circ: 0.95217
Ran for 150550 frames Cell Events: 6807 mean_circ: 0.952164
Ran for 151550 frames Cell Events: 6847 mean_circ: 0.952199
Ran for 152050 frames Cell Events: 6876 mean_circ: 0.952189
Ran for 153050 frames Cell Events: 6919 mean_circ: 0.952188
Ran for 155050 frames Cell Events: 6979 mean_circ: 0.952195
Ran for 156550 frames Cell Events: 7050 mean_circ: 0.952195
Ran for 158050 frames Cell Events: 7120 mean_circ: 0.952233
Ran for 158550 frames Cell Events: 7146 mean_circ: 0.952233
Ran for 160050 frames Cell Events: 7210 mean_circ: 0.952246
Ran for 163050 frames Cell Events: 7351 mean_circ: 0.95219
Ran for 164550 frames Cell Events: 7416 mean_circ: 0.95216
Ran for 165550 frames Cell Events: 7449 mean_circ: 0.952156
Ran for 166050 frames Cell Events: 7473 mean_circ: 0.952194
Ran for 168050 frames Cell Events: 7585 mean_circ: 0.95216
Ran for 168550 frames Cell Events: 7604 mean_circ: 0.95216
Ran for 170050 frames Cell Events: 7675 mean_circ: 0.952125
Ran for 170550 frames Cell Events: 7701 mean_circ: 0.95212
Ran for 172550 frames Cell Events: 7806 mean_circ: 0.952127
Ran for 173050 frames Cell Events: 7831 mean_circ: 0.952155
Ran for 174550 frames Cell Events: 7889 mean_circ: 0.952188
Ran for 175550 frames Cell Events: 7930 mean_circ: 0.952221
Ran for 176050 frames Cell Events: 7954 mean_circ: 0.952241
Ran for 178550 frames Cell Events: 8063 mean_circ: 0.952228



End this measurment..



Stopping time: 1.75374e+009
****************************************
Measurement finished

Mean Circularitiy: 0.952105
Mean Area: 571.665
Mean FL-1 intensity: 3380.2
Mean FL-2 intensity: 3781.91
Mean FL-3 intensity: 0

frames processed: 175017
Total Time: 89340.6ms
Number of Runs (new bg img) 0
Frames collected: 178562
Skipped frames: 54121
Rescued Frames: 1394678
Samples DAQ: 44640853
Samples written: 89280748
Samples per Channel: 44640374
FPS: 1998.67

Samples per second: 499665
Expected samples/s: 500000

Events: 8064
Events per second: 90.2614
jumped back (PMT): 3138
#Frames read, frequency
# 0 frames :284599
# 1 frames :58772
# 2 frames :2862
# 3 frames :1412
# 4 frames :816
# 5 frames :510
# 6 frames :763
# 7 frames :1112
# 8 frames :1073
# 9 frames :775
# 10 frames :578
# 11 frames :469
# 12 frames :368
# 13 frames :310
# 14 frames :271
# 15 frames :252
# 16 frames :237
# 17 frames :236
# 18 frames :173
# 19 frames :163
# 20 frames :135
# 21 frames :136
# 22 frames :98
# 23 frames :99
# 24 frames :63
# 25 frames :75
# 26 frames :56
# 27 frames :52
# 28 frames :59
# 29 frames :35
# 30 frames :38
# 31 frames :32
# 32 frames :42
# 33 frames :26
# 34 frames :31
# 35 frames :22
# 36 frames :28
# 37 frames :18
# 38 frames :11
# 39 frames :22
# 40 frames :22
# 41 frames :6
# 42 frames :7
# 43 frames :9
# 44 frames :7
# 45 frames :8
# 46 frames :9
# 47 frames :5
# 48 frames :4
# 49 frames :1
# 50 frames :4
# 51 frames :8
# 52 frames :1
# 53 frames :3
# 54 frames :2
# 55 frames :4
# 56 frames :1
# 57 frames :4
# 58 frames :2
# 59 frames :2
# 60 frames :1
# 61 frames :1
# 62 frames :1
# 63 frames :1
# 64 frames :3
# 67 frames :3
# 69 frames :1
# 78 frames :2
# 79 frames :1
# 89 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.0378
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0