Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_12.40.22 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "66f3d11142c23e596421d58b91875a81",
"name": "M1_20170604_1556_dsRNA_G2_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "10:40:44"
}
}
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.056
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.75522e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 50 frames Cell Events: 0 mean_circ: 0.948463
Ran for 550 frames Cell Events: 24 mean_circ: 0.937026
Ran for 2050 frames Cell Events: 93 mean_circ: 0.933495
Ran for 2550 frames Cell Events: 124 mean_circ: 0.931699
Ran for 4050 frames Cell Events: 228 mean_circ: 0.934414
Ran for 5050 frames Cell Events: 250 mean_circ: 0.935333
Ran for 5550 frames Cell Events: 266 mean_circ: 0.935274
Ran for 6050 frames Cell Events: 289 mean_circ: 0.934406
Ran for 6550 frames Cell Events: 316 mean_circ: 0.934941
Ran for 8050 frames Cell Events: 381 mean_circ: 0.93446
Ran for 9050 frames Cell Events: 430 mean_circ: 0.935319
Ran for 10550 frames Cell Events: 499 mean_circ: 0.935043
Ran for 11050 frames Cell Events: 521 mean_circ: 0.93515
Ran for 11550 frames Cell Events: 541 mean_circ: 0.935947
Ran for 12050 frames Cell Events: 560 mean_circ: 0.936631
Ran for 12550 frames Cell Events: 580 mean_circ: 0.936318
Ran for 14050 frames Cell Events: 658 mean_circ: 0.936774
Ran for 15550 frames Cell Events: 719 mean_circ: 0.937571
Ran for 16050 frames Cell Events: 739 mean_circ: 0.938061
Ran for 16550 frames Cell Events: 761 mean_circ: 0.938341
Ran for 18050 frames Cell Events: 836 mean_circ: 0.937741
Ran for 18550 frames Cell Events: 856 mean_circ: 0.937716
Ran for 19050 frames Cell Events: 883 mean_circ: 0.937626
Ran for 20050 frames Cell Events: 922 mean_circ: 0.937813
Ran for 20550 frames Cell Events: 939 mean_circ: 0.937998
Ran for 21050 frames Cell Events: 960 mean_circ: 0.938011
Ran for 21550 frames Cell Events: 984 mean_circ: 0.938054
Ran for 22050 frames Cell Events: 1010 mean_circ: 0.937527
Ran for 23550 frames Cell Events: 1084 mean_circ: 0.937806
Ran for 24050 frames Cell Events: 1106 mean_circ: 0.938011
Ran for 24550 frames Cell Events: 1131 mean_circ: 0.938112
Ran for 25050 frames Cell Events: 1157 mean_circ: 0.93789
Ran for 25550 frames Cell Events: 1200 mean_circ: 0.937665
Ran for 26050 frames Cell Events: 1226 mean_circ: 0.937487
Ran for 27050 frames Cell Events: 1276 mean_circ: 0.937581
Ran for 28050 frames Cell Events: 1331 mean_circ: 0.9377
Ran for 28550 frames Cell Events: 1373 mean_circ: 0.937344
Ran for 29550 frames Cell Events: 1423 mean_circ: 0.937404
Ran for 32050 frames Cell Events: 1539 mean_circ: 0.937034
Ran for 37050 frames Cell Events: 1769 mean_circ: 0.936993
Ran for 37550 frames Cell Events: 1786 mean_circ: 0.93683
Ran for 38050 frames Cell Events: 1813 mean_circ: 0.936811
Ran for 40550 frames Cell Events: 1947 mean_circ: 0.936988
Ran for 41050 frames Cell Events: 1971 mean_circ: 0.937009
Ran for 42050 frames Cell Events: 2034 mean_circ: 0.937034
Ran for 42550 frames Cell Events: 2053 mean_circ: 0.93717
Ran for 43050 frames Cell Events: 2073 mean_circ: 0.93718
Ran for 43550 frames Cell Events: 2089 mean_circ: 0.937127
Ran for 45050 frames Cell Events: 2162 mean_circ: 0.936863
Ran for 45550 frames Cell Events: 2183 mean_circ: 0.936908
Ran for 46050 frames Cell Events: 2196 mean_circ: 0.936985
Ran for 48050 frames Cell Events: 2289 mean_circ: 0.936805
Ran for 49050 frames Cell Events: 2329 mean_circ: 0.936994
Ran for 49550 frames Cell Events: 2360 mean_circ: 0.936977
Ran for 50050 frames Cell Events: 2382 mean_circ: 0.937
Ran for 51050 frames Cell Events: 2430 mean_circ: 0.936895
Ran for 52550 frames Cell Events: 2504 mean_circ: 0.936767
Ran for 53050 frames Cell Events: 2528 mean_circ: 0.936702
Ran for 54050 frames Cell Events: 2576 mean_circ: 0.936892
Ran for 55050 frames Cell Events: 2608 mean_circ: 0.936765
Ran for 55550 frames Cell Events: 2642 mean_circ: 0.936734
Ran for 57550 frames Cell Events: 2707 mean_circ: 0.936576
Ran for 58050 frames Cell Events: 2720 mean_circ: 0.936607
Ran for 58550 frames Cell Events: 2748 mean_circ: 0.93671
Ran for 60550 frames Cell Events: 2840 mean_circ: 0.936586
Ran for 61050 frames Cell Events: 2874 mean_circ: 0.936636
Ran for 61550 frames Cell Events: 2889 mean_circ: 0.936693
Ran for 62050 frames Cell Events: 2909 mean_circ: 0.936689
Ran for 62550 frames Cell Events: 2940 mean_circ: 0.936746
Ran for 63050 frames Cell Events: 2962 mean_circ: 0.936786
Ran for 63550 frames Cell Events: 2987 mean_circ: 0.936769
Ran for 65550 frames Cell Events: 3092 mean_circ: 0.936886
Ran for 66550 frames Cell Events: 3135 mean_circ: 0.936923
Ran for 67050 frames Cell Events: 3157 mean_circ: 0.936909
Ran for 67550 frames Cell Events: 3182 mean_circ: 0.936822
Ran for 70050 frames Cell Events: 3283 mean_circ: 0.936782
Ran for 70550 frames Cell Events: 3309 mean_circ: 0.936812
Ran for 71050 frames Cell Events: 3332 mean_circ: 0.936803
Ran for 72050 frames Cell Events: 3382 mean_circ: 0.936773
Ran for 74550 frames Cell Events: 3504 mean_circ: 0.936578
Ran for 75550 frames Cell Events: 3580 mean_circ: 0.936658
Ran for 76550 frames Cell Events: 3622 mean_circ: 0.936578
Ran for 77050 frames Cell Events: 3646 mean_circ: 0.93662
Ran for 77550 frames Cell Events: 3680 mean_circ: 0.936545
Ran for 78050 frames Cell Events: 3701 mean_circ: 0.93649
Ran for 78550 frames Cell Events: 3717 mean_circ: 0.936509
Ran for 79050 frames Cell Events: 3738 mean_circ: 0.936476
Ran for 82050 frames Cell Events: 3847 mean_circ: 0.936324
Ran for 82550 frames Cell Events: 3865 mean_circ: 0.936307
Ran for 85550 frames Cell Events: 3984 mean_circ: 0.936127
Ran for 86050 frames Cell Events: 4002 mean_circ: 0.936118
Ran for 86550 frames Cell Events: 4018 mean_circ: 0.936102
Ran for 87050 frames Cell Events: 4045 mean_circ: 0.936126
Ran for 88550 frames Cell Events: 4119 mean_circ: 0.936223
Ran for 89050 frames Cell Events: 4145 mean_circ: 0.936148
Ran for 90050 frames Cell Events: 4186 mean_circ: 0.936222
Ran for 91550 frames Cell Events: 4254 mean_circ: 0.936161
Ran for 92050 frames Cell Events: 4272 mean_circ: 0.936207
Ran for 94050 frames Cell Events: 4364 mean_circ: 0.936214
Ran for 94550 frames Cell Events: 4386 mean_circ: 0.936236
Ran for 95550 frames Cell Events: 4439 mean_circ: 0.936239
Ran for 96050 frames Cell Events: 4457 mean_circ: 0.936211
Ran for 96550 frames Cell Events: 4475 mean_circ: 0.936216
Ran for 98050 frames Cell Events: 4529 mean_circ: 0.936159
Ran for 98550 frames Cell Events: 4547 mean_circ: 0.936124
Ran for 99550 frames Cell Events: 4608 mean_circ: 0.93613
Ran for 101050 frames Cell Events: 4692 mean_circ: 0.936145
Ran for 101550 frames Cell Events: 4717 mean_circ: 0.93614
Ran for 102550 frames Cell Events: 4772 mean_circ: 0.936081
Ran for 103050 frames Cell Events: 4804 mean_circ: 0.936016
Ran for 104050 frames Cell Events: 4871 mean_circ: 0.936063
Ran for 107550 frames Cell Events: 5071 mean_circ: 0.936129
Ran for 109050 frames Cell Events: 5154 mean_circ: 0.936104
Ran for 110050 frames Cell Events: 5202 mean_circ: 0.936155
Ran for 111050 frames Cell Events: 5264 mean_circ: 0.936121
Ran for 112050 frames Cell Events: 5320 mean_circ: 0.936112
Ran for 113550 frames Cell Events: 5402 mean_circ: 0.936134
Ran for 114050 frames Cell Events: 5429 mean_circ: 0.936167
Ran for 114550 frames Cell Events: 5451 mean_circ: 0.936197
Ran for 115550 frames Cell Events: 5501 mean_circ: 0.936254
Ran for 118050 frames Cell Events: 5636 mean_circ: 0.936269
Ran for 118550 frames Cell Events: 5667 mean_circ: 0.936284
Ran for 119050 frames Cell Events: 5689 mean_circ: 0.936316
Ran for 121050 frames Cell Events: 5777 mean_circ: 0.936244
Ran for 123550 frames Cell Events: 5921 mean_circ: 0.93632
Ran for 124050 frames Cell Events: 5943 mean_circ: 0.936297
Ran for 124550 frames Cell Events: 5959 mean_circ: 0.936267
Ran for 125550 frames Cell Events: 6003 mean_circ: 0.936318
Ran for 128550 frames Cell Events: 6173 mean_circ: 0.936414
Ran for 129050 frames Cell Events: 6203 mean_circ: 0.936414
Ran for 129550 frames Cell Events: 6220 mean_circ: 0.936446
Ran for 130050 frames Cell Events: 6248 mean_circ: 0.93637
Ran for 134550 frames Cell Events: 6455 mean_circ: 0.936332
Ran for 135550 frames Cell Events: 6490 mean_circ: 0.936387
Ran for 136050 frames Cell Events: 6509 mean_circ: 0.936401
Ran for 136550 frames Cell Events: 6531 mean_circ: 0.936397
Ran for 137050 frames Cell Events: 6546 mean_circ: 0.936413
Ran for 137550 frames Cell Events: 6571 mean_circ: 0.936398
Ran for 139050 frames Cell Events: 6651 mean_circ: 0.93642
Ran for 139550 frames Cell Events: 6683 mean_circ: 0.936373
Ran for 140050 frames Cell Events: 6706 mean_circ: 0.936375
Ran for 140550 frames Cell Events: 6738 mean_circ: 0.936402
Ran for 144050 frames Cell Events: 6962 mean_circ: 0.936238
Ran for 145050 frames Cell Events: 7033 mean_circ: 0.936208
Ran for 146550 frames Cell Events: 7125 mean_circ: 0.936177
Ran for 147550 frames Cell Events: 7176 mean_circ: 0.936172
Ran for 148050 frames Cell Events: 7207 mean_circ: 0.936153
Ran for 149050 frames Cell Events: 7258 mean_circ: 0.936178
Ran for 149550 frames Cell Events: 7269 mean_circ: 0.93618
Ran for 151050 frames Cell Events: 7341 mean_circ: 0.93612
Ran for 152050 frames Cell Events: 7398 mean_circ: 0.936146
Ran for 154050 frames Cell Events: 7491 mean_circ: 0.936168
Ran for 155050 frames Cell Events: 7555 mean_circ: 0.936194
Ran for 155550 frames Cell Events: 7583 mean_circ: 0.936197
Ran for 157050 frames Cell Events: 7652 mean_circ: 0.936206
Ran for 157550 frames Cell Events: 7684 mean_circ: 0.936147
Ran for 158050 frames Cell Events: 7706 mean_circ: 0.936143
Ran for 158550 frames Cell Events: 7733 mean_circ: 0.93615
Ran for 159050 frames Cell Events: 7756 mean_circ: 0.936154
Ran for 160550 frames Cell Events: 7831 mean_circ: 0.936184
Ran for 161050 frames Cell Events: 7852 mean_circ: 0.936185
Ran for 161550 frames Cell Events: 7881 mean_circ: 0.936208
Ran for 162050 frames Cell Events: 7906 mean_circ: 0.9362
Ran for 163550 frames Cell Events: 7996 mean_circ: 0.936257
Ran for 165550 frames Cell Events: 8117 mean_circ: 0.936247
Ran for 166550 frames Cell Events: 8175 mean_circ: 0.936196
Ran for 167050 frames Cell Events: 8207 mean_circ: 0.936209
Ran for 170050 frames Cell Events: 8392 mean_circ: 0.936086
Ran for 171550 frames Cell Events: 8442 mean_circ: 0.936138
Ran for 172550 frames Cell Events: 8488 mean_circ: 0.9362
Ran for 173050 frames Cell Events: 8507 mean_circ: 0.93615



End this measurment..



Stopping time: 1.75531e+009
****************************************
Measurement finished

Mean Circularitiy: 0.936093
Mean Area: 617.865
Mean FL-1 intensity: 1613.29
Mean FL-2 intensity: 2466.08
Mean FL-3 intensity: 0

frames processed: 172195
Total Time: 87341.1ms
Number of Runs (new bg img) 0
Frames collected: 174531
Skipped frames: 66061
Rescued Frames: 1873874
Samples DAQ: 43640418
Samples written: 87265614
Samples per Channel: 43632807
FPS: 1998.27

Samples per second: 499568
Expected samples/s: 500000

Events: 8590
Events per second: 98.35
jumped back (PMT): 30
#Frames read, frequency
# 0 frames :267148
# 1 frames :67174
# 2 frames :3555
# 3 frames :1366
# 4 frames :815
# 5 frames :469
# 6 frames :1636
# 7 frames :1426
# 8 frames :1381
# 9 frames :840
# 10 frames :573
# 11 frames :390
# 12 frames :313
# 13 frames :240
# 14 frames :210
# 15 frames :208
# 16 frames :174
# 17 frames :168
# 18 frames :126
# 19 frames :113
# 20 frames :91
# 21 frames :74
# 22 frames :55
# 23 frames :55
# 24 frames :50
# 25 frames :49
# 26 frames :37
# 27 frames :37
# 28 frames :24
# 29 frames :31
# 30 frames :13
# 31 frames :17
# 32 frames :18
# 33 frames :14
# 34 frames :3
# 35 frames :11
# 36 frames :15
# 37 frames :8
# 38 frames :7
# 39 frames :6
# 40 frames :8
# 41 frames :6
# 42 frames :1
# 43 frames :2
# 44 frames :2
# 45 frames :3
# 47 frames :2
# 48 frames :1
# 49 frames :2
# 50 frames :1
# 51 frames :3
# 53 frames :2
# 57 frames :2
# 58 frames :1
# 59 frames :1
# 61 frames :1
# 67 frames :1
# 68 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.056
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0