Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_12.42.10 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "9d62f7ca5f27e392ae89eac476556a4f",
"name": "M1_20170604_1620_dsRNA_E6_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "10:42:30"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8130 (max 8131) in '8130'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.2053
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.75664e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 50 frames Cell Events: 1 mean_circ: 0.949117
Ran for 550 frames Cell Events: 19 mean_circ: 0.943498
Ran for 1050 frames Cell Events: 36 mean_circ: 0.945357
Ran for 2550 frames Cell Events: 93 mean_circ: 0.941698
Ran for 3050 frames Cell Events: 112 mean_circ: 0.938983
Ran for 3550 frames Cell Events: 139 mean_circ: 0.940341
Ran for 5050 frames Cell Events: 207 mean_circ: 0.942635
Ran for 6050 frames Cell Events: 258 mean_circ: 0.941401
Ran for 6550 frames Cell Events: 282 mean_circ: 0.941555
Ran for 7050 frames Cell Events: 305 mean_circ: 0.939619
Ran for 7550 frames Cell Events: 334 mean_circ: 0.939773
Ran for 9050 frames Cell Events: 398 mean_circ: 0.940392
Ran for 9550 frames Cell Events: 431 mean_circ: 0.940565
Ran for 11550 frames Cell Events: 485 mean_circ: 0.940548
Ran for 12050 frames Cell Events: 510 mean_circ: 0.940548
Ran for 12550 frames Cell Events: 538 mean_circ: 0.940548
Ran for 13050 frames Cell Events: 555 mean_circ: 0.939416
Ran for 13550 frames Cell Events: 580 mean_circ: 0.939224
Ran for 14050 frames Cell Events: 606 mean_circ: 0.939487
Ran for 15050 frames Cell Events: 650 mean_circ: 0.939955
Ran for 15550 frames Cell Events: 668 mean_circ: 0.940272
Ran for 16050 frames Cell Events: 687 mean_circ: 0.939955
Ran for 16550 frames Cell Events: 719 mean_circ: 0.939955
Ran for 17050 frames Cell Events: 743 mean_circ: 0.939757
Ran for 18550 frames Cell Events: 817 mean_circ: 0.939914
Ran for 19050 frames Cell Events: 846 mean_circ: 0.939767
Ran for 19550 frames Cell Events: 873 mean_circ: 0.939331
Ran for 20050 frames Cell Events: 886 mean_circ: 0.939407
Ran for 21550 frames Cell Events: 967 mean_circ: 0.9398
Ran for 22550 frames Cell Events: 1015 mean_circ: 0.939219
Ran for 24050 frames Cell Events: 1083 mean_circ: 0.939189
Ran for 24550 frames Cell Events: 1112 mean_circ: 0.938977
Ran for 26050 frames Cell Events: 1196 mean_circ: 0.938592
Ran for 27550 frames Cell Events: 1249 mean_circ: 0.938533
Ran for 28050 frames Cell Events: 1268 mean_circ: 0.938619
Ran for 29050 frames Cell Events: 1316 mean_circ: 0.938586
Ran for 29550 frames Cell Events: 1342 mean_circ: 0.938639
Ran for 31550 frames Cell Events: 1445 mean_circ: 0.938963
Ran for 32550 frames Cell Events: 1499 mean_circ: 0.938583
Ran for 33050 frames Cell Events: 1526 mean_circ: 0.938433
Ran for 35050 frames Cell Events: 1625 mean_circ: 0.938516
Ran for 36550 frames Cell Events: 1710 mean_circ: 0.938511
Ran for 37550 frames Cell Events: 1775 mean_circ: 0.937919
Ran for 38550 frames Cell Events: 1814 mean_circ: 0.937837
Ran for 39050 frames Cell Events: 1841 mean_circ: 0.937749
Ran for 39550 frames Cell Events: 1867 mean_circ: 0.937855
Ran for 40050 frames Cell Events: 1898 mean_circ: 0.937626
Ran for 42050 frames Cell Events: 2000 mean_circ: 0.937488
Ran for 42550 frames Cell Events: 2023 mean_circ: 0.937549
Ran for 44050 frames Cell Events: 2104 mean_circ: 0.937475
Ran for 44550 frames Cell Events: 2131 mean_circ: 0.937594
Ran for 46050 frames Cell Events: 2206 mean_circ: 0.937555
Ran for 46550 frames Cell Events: 2228 mean_circ: 0.937569
Ran for 48050 frames Cell Events: 2297 mean_circ: 0.9375
Ran for 48550 frames Cell Events: 2327 mean_circ: 0.937381
Ran for 49050 frames Cell Events: 2355 mean_circ: 0.937393
Ran for 49550 frames Cell Events: 2378 mean_circ: 0.937402
Ran for 50050 frames Cell Events: 2400 mean_circ: 0.937323
Ran for 50550 frames Cell Events: 2431 mean_circ: 0.937294
Ran for 51050 frames Cell Events: 2449 mean_circ: 0.937218
Ran for 52050 frames Cell Events: 2501 mean_circ: 0.937515
Ran for 52550 frames Cell Events: 2524 mean_circ: 0.937448
Ran for 53050 frames Cell Events: 2542 mean_circ: 0.937456
Ran for 53550 frames Cell Events: 2561 mean_circ: 0.937478
Ran for 55550 frames Cell Events: 2661 mean_circ: 0.937598
Ran for 56550 frames Cell Events: 2705 mean_circ: 0.937468
Ran for 57050 frames Cell Events: 2729 mean_circ: 0.937418
Ran for 59550 frames Cell Events: 2865 mean_circ: 0.937528
Ran for 61550 frames Cell Events: 2978 mean_circ: 0.937715
Ran for 62050 frames Cell Events: 3009 mean_circ: 0.937715
Ran for 64050 frames Cell Events: 3121 mean_circ: 0.937771
Ran for 64550 frames Cell Events: 3146 mean_circ: 0.937779
Ran for 65050 frames Cell Events: 3162 mean_circ: 0.93781
Ran for 65550 frames Cell Events: 3185 mean_circ: 0.937829
Ran for 67050 frames Cell Events: 3249 mean_circ: 0.937956
Ran for 68050 frames Cell Events: 3309 mean_circ: 0.93804
Ran for 69550 frames Cell Events: 3389 mean_circ: 0.938143
Ran for 70050 frames Cell Events: 3422 mean_circ: 0.938171
Ran for 70550 frames Cell Events: 3438 mean_circ: 0.938165
Ran for 72050 frames Cell Events: 3472 mean_circ: 0.938194
Ran for 72550 frames Cell Events: 3503 mean_circ: 0.938178
Ran for 73050 frames Cell Events: 3528 mean_circ: 0.93816
Ran for 74550 frames Cell Events: 3583 mean_circ: 0.938264
Ran for 75550 frames Cell Events: 3600 mean_circ: 0.93821
Ran for 76050 frames Cell Events: 3632 mean_circ: 0.938098
Ran for 77550 frames Cell Events: 3724 mean_circ: 0.938185
Ran for 78050 frames Cell Events: 3744 mean_circ: 0.938212
Ran for 78550 frames Cell Events: 3784 mean_circ: 0.938252
Ran for 79050 frames Cell Events: 3806 mean_circ: 0.93825
Ran for 80550 frames Cell Events: 3877 mean_circ: 0.938219
Ran for 82550 frames Cell Events: 3975 mean_circ: 0.938396
Ran for 83550 frames Cell Events: 4035 mean_circ: 0.938387
Ran for 84050 frames Cell Events: 4054 mean_circ: 0.938388
Ran for 84550 frames Cell Events: 4078 mean_circ: 0.938399
Ran for 85550 frames Cell Events: 4119 mean_circ: 0.938384
Ran for 86050 frames Cell Events: 4140 mean_circ: 0.938419
Ran for 86550 frames Cell Events: 4156 mean_circ: 0.938385
Ran for 87050 frames Cell Events: 4186 mean_circ: 0.938405
Ran for 88050 frames Cell Events: 4219 mean_circ: 0.938326
Ran for 88550 frames Cell Events: 4240 mean_circ: 0.938312
Ran for 89550 frames Cell Events: 4271 mean_circ: 0.938296
Ran for 90050 frames Cell Events: 4295 mean_circ: 0.938342
Ran for 90550 frames Cell Events: 4320 mean_circ: 0.93827
Ran for 91550 frames Cell Events: 4365 mean_circ: 0.938256
Ran for 94050 frames Cell Events: 4457 mean_circ: 0.938196
Ran for 94550 frames Cell Events: 4497 mean_circ: 0.938235
Ran for 95050 frames Cell Events: 4523 mean_circ: 0.938247
Ran for 95550 frames Cell Events: 4547 mean_circ: 0.938223
Ran for 96050 frames Cell Events: 4559 mean_circ: 0.938213
Ran for 96550 frames Cell Events: 4582 mean_circ: 0.938251
Ran for 97550 frames Cell Events: 4645 mean_circ: 0.938219
Ran for 98050 frames Cell Events: 4668 mean_circ: 0.938284
Ran for 99550 frames Cell Events: 4750 mean_circ: 0.938275
Ran for 100550 frames Cell Events: 4807 mean_circ: 0.938279
Ran for 101050 frames Cell Events: 4828 mean_circ: 0.938237
Ran for 102550 frames Cell Events: 4900 mean_circ: 0.93819
Ran for 105550 frames Cell Events: 5041 mean_circ: 0.938229
Ran for 107550 frames Cell Events: 5137 mean_circ: 0.938029
Ran for 108050 frames Cell Events: 5171 mean_circ: 0.938029
Ran for 108550 frames Cell Events: 5197 mean_circ: 0.938032
Ran for 113050 frames Cell Events: 5441 mean_circ: 0.938079
Ran for 113550 frames Cell Events: 5479 mean_circ: 0.938045
Ran for 114550 frames Cell Events: 5534 mean_circ: 0.938082
Ran for 115050 frames Cell Events: 5568 mean_circ: 0.938082
Ran for 116050 frames Cell Events: 5606 mean_circ: 0.93806
Ran for 116550 frames Cell Events: 5635 mean_circ: 0.938019
Ran for 118550 frames Cell Events: 5734 mean_circ: 0.937925
Ran for 119050 frames Cell Events: 5760 mean_circ: 0.937859
Ran for 119550 frames Cell Events: 5778 mean_circ: 0.937869
Ran for 121050 frames Cell Events: 5866 mean_circ: 0.937953
Ran for 122050 frames Cell Events: 5920 mean_circ: 0.937931
Ran for 123050 frames Cell Events: 5970 mean_circ: 0.937877
Ran for 123550 frames Cell Events: 5987 mean_circ: 0.937894
Ran for 124050 frames Cell Events: 6014 mean_circ: 0.937943
Ran for 124550 frames Cell Events: 6035 mean_circ: 0.937982
Ran for 125050 frames Cell Events: 6059 mean_circ: 0.937976
Ran for 126050 frames Cell Events: 6107 mean_circ: 0.93799
Ran for 126550 frames Cell Events: 6128 mean_circ: 0.938002
Ran for 127050 frames Cell Events: 6154 mean_circ: 0.937987
Ran for 127550 frames Cell Events: 6181 mean_circ: 0.937921
Ran for 128050 frames Cell Events: 6215 mean_circ: 0.937921
Ran for 128550 frames Cell Events: 6241 mean_circ: 0.938035
Ran for 129050 frames Cell Events: 6267 mean_circ: 0.938091
Ran for 130050 frames Cell Events: 6314 mean_circ: 0.938163
Ran for 130550 frames Cell Events: 6343 mean_circ: 0.93815
Ran for 131550 frames Cell Events: 6378 mean_circ: 0.938168
Ran for 134050 frames Cell Events: 6525 mean_circ: 0.938135
Ran for 135050 frames Cell Events: 6570 mean_circ: 0.938184
Ran for 136550 frames Cell Events: 6612 mean_circ: 0.938214
Ran for 139050 frames Cell Events: 6734 mean_circ: 0.938026
Ran for 140050 frames Cell Events: 6785 mean_circ: 0.937989
Ran for 141050 frames Cell Events: 6831 mean_circ: 0.93798
Ran for 142050 frames Cell Events: 6859 mean_circ: 0.937957
Ran for 143550 frames Cell Events: 6936 mean_circ: 0.937904
Ran for 144050 frames Cell Events: 6967 mean_circ: 0.937941
Ran for 145050 frames Cell Events: 7005 mean_circ: 0.937919
Ran for 146550 frames Cell Events: 7066 mean_circ: 0.937874
Ran for 147050 frames Cell Events: 7085 mean_circ: 0.937874
Ran for 147550 frames Cell Events: 7107 mean_circ: 0.937847
Ran for 148550 frames Cell Events: 7164 mean_circ: 0.937765
Ran for 151550 frames Cell Events: 7314 mean_circ: 0.937668
Ran for 153050 frames Cell Events: 7376 mean_circ: 0.937662
Ran for 153550 frames Cell Events: 7397 mean_circ: 0.937662
Ran for 154550 frames Cell Events: 7449 mean_circ: 0.937667
Ran for 155050 frames Cell Events: 7480 mean_circ: 0.937667
Ran for 156050 frames Cell Events: 7527 mean_circ: 0.937601
Ran for 157050 frames Cell Events: 7590 mean_circ: 0.937623
Ran for 158050 frames Cell Events: 7638 mean_circ: 0.937618
Ran for 158550 frames Cell Events: 7662 mean_circ: 0.937576
Ran for 162550 frames Cell Events: 7902 mean_circ: 0.937639
Ran for 163050 frames Cell Events: 7928 mean_circ: 0.93756
Ran for 164550 frames Cell Events: 8000 mean_circ: 0.937559
Ran for 165050 frames Cell Events: 8026 mean_circ: 0.937537
Ran for 165550 frames Cell Events: 8052 mean_circ: 0.937551
Ran for 166550 frames Cell Events: 8085 mean_circ: 0.937606
Ran for 167050 frames Cell Events: 8102 mean_circ: 0.937612
Ran for 167550 frames Cell Events: 8131 mean_circ: 0.937617



End this measurment..



Stopping time: 1.75672e+009
****************************************
Measurement finished

Mean Circularitiy: 0.937621
Mean Area: 592.049
Mean FL-1 intensity: 3321.45
Mean FL-2 intensity: 3533.2
Mean FL-3 intensity: 0

frames processed: 162276
Total Time: 83843.2ms
Number of Runs (new bg img) 0
Frames collected: 167566
Skipped frames: 76133
Rescued Frames: 2245829
Samples DAQ: 41891467
Samples written: 83782724
Samples per Channel: 41891362
FPS: 1998.56

Samples per second: 499639
Expected samples/s: 500000

Events: 8131
Events per second: 96.9786
jumped back (PMT): 35
#Frames read, frequency
# 0 frames :261783
# 1 frames :59564
# 2 frames :3136
# 3 frames :1272
# 4 frames :728
# 5 frames :414
# 6 frames :1393
# 7 frames :1242
# 8 frames :1116
# 9 frames :746
# 10 frames :620
# 11 frames :410
# 12 frames :347
# 13 frames :254
# 14 frames :233
# 15 frames :223
# 16 frames :201
# 17 frames :143
# 18 frames :137
# 19 frames :125
# 20 frames :82
# 21 frames :97
# 22 frames :71
# 23 frames :56
# 24 frames :56
# 25 frames :45
# 26 frames :30
# 27 frames :34
# 28 frames :40
# 29 frames :24
# 30 frames :31
# 31 frames :22
# 32 frames :18
# 33 frames :19
# 34 frames :21
# 35 frames :7
# 36 frames :11
# 37 frames :7
# 38 frames :13
# 39 frames :3
# 40 frames :11
# 41 frames :6
# 42 frames :5
# 43 frames :7
# 44 frames :6
# 45 frames :2
# 46 frames :3
# 47 frames :1
# 48 frames :3
# 49 frames :3
# 50 frames :2
# 51 frames :3
# 52 frames :1
# 53 frames :4
# 54 frames :3
# 55 frames :2
# 56 frames :1
# 57 frames :1
# 59 frames :1
# 60 frames :1
# 62 frames :1
# 64 frames :1
# 66 frames :2
# 67 frames :1
# 69 frames :1
# 72 frames :1
# 82 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.2053
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0