Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_12.52.12 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "84a42c87bf2535c50cd78d79e7e4ab8a",
"name": "M1_20170604_1840_dsRNA_E3_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "10:52:32"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8141 (max 8142) in '8141'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.2459
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.76503e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 64 mean_circ: 0.933603
Ran for 2050 frames Cell Events: 121 mean_circ: 0.933223
Ran for 3550 frames Cell Events: 215 mean_circ: 0.930706
Ran for 4550 frames Cell Events: 294 mean_circ: 0.927985
Ran for 6550 frames Cell Events: 420 mean_circ: 0.927911
Ran for 7550 frames Cell Events: 479 mean_circ: 0.928572
Ran for 9050 frames Cell Events: 578 mean_circ: 0.929531
Ran for 10550 frames Cell Events: 685 mean_circ: 0.930845
Ran for 12050 frames Cell Events: 782 mean_circ: 0.93171
Ran for 12550 frames Cell Events: 812 mean_circ: 0.931165
Ran for 13050 frames Cell Events: 843 mean_circ: 0.930867
Ran for 13550 frames Cell Events: 884 mean_circ: 0.931218
Ran for 14050 frames Cell Events: 917 mean_circ: 0.931218
Ran for 15050 frames Cell Events: 970 mean_circ: 0.931158
Ran for 16050 frames Cell Events: 997 mean_circ: 0.931269
Ran for 16550 frames Cell Events: 1036 mean_circ: 0.931467
Ran for 17550 frames Cell Events: 1081 mean_circ: 0.931805
Ran for 18550 frames Cell Events: 1139 mean_circ: 0.931933
Ran for 19550 frames Cell Events: 1198 mean_circ: 0.931828
Ran for 20550 frames Cell Events: 1244 mean_circ: 0.931518
Ran for 23550 frames Cell Events: 1471 mean_circ: 0.931015
Ran for 25050 frames Cell Events: 1583 mean_circ: 0.930994
Ran for 25550 frames Cell Events: 1619 mean_circ: 0.93095
Ran for 26550 frames Cell Events: 1688 mean_circ: 0.931301
Ran for 27050 frames Cell Events: 1710 mean_circ: 0.931316
Ran for 31550 frames Cell Events: 2076 mean_circ: 0.931743
Ran for 32550 frames Cell Events: 2141 mean_circ: 0.931718
Ran for 33550 frames Cell Events: 2205 mean_circ: 0.931614
Ran for 34050 frames Cell Events: 2225 mean_circ: 0.931678
Ran for 34550 frames Cell Events: 2243 mean_circ: 0.931718
Ran for 35550 frames Cell Events: 2293 mean_circ: 0.931853
Ran for 36550 frames Cell Events: 2345 mean_circ: 0.931823
Ran for 37050 frames Cell Events: 2368 mean_circ: 0.93181
Ran for 38050 frames Cell Events: 2413 mean_circ: 0.931598
Ran for 39050 frames Cell Events: 2457 mean_circ: 0.931543
Ran for 39550 frames Cell Events: 2495 mean_circ: 0.931438
Ran for 41550 frames Cell Events: 2625 mean_circ: 0.931544
Ran for 42050 frames Cell Events: 2653 mean_circ: 0.931635
Ran for 43550 frames Cell Events: 2737 mean_circ: 0.931629
Ran for 44050 frames Cell Events: 2758 mean_circ: 0.931631
Ran for 45050 frames Cell Events: 2810 mean_circ: 0.931581
Ran for 47050 frames Cell Events: 2930 mean_circ: 0.931509
Ran for 49050 frames Cell Events: 3045 mean_circ: 0.931848
Ran for 49550 frames Cell Events: 3075 mean_circ: 0.931898
Ran for 50050 frames Cell Events: 3112 mean_circ: 0.93203
Ran for 50550 frames Cell Events: 3137 mean_circ: 0.932022
Ran for 51050 frames Cell Events: 3171 mean_circ: 0.932064
Ran for 51550 frames Cell Events: 3209 mean_circ: 0.932116
Ran for 52050 frames Cell Events: 3245 mean_circ: 0.932139
Ran for 53550 frames Cell Events: 3337 mean_circ: 0.931862
Ran for 54050 frames Cell Events: 3369 mean_circ: 0.931859
Ran for 54550 frames Cell Events: 3409 mean_circ: 0.931757
Ran for 56550 frames Cell Events: 3535 mean_circ: 0.93176
Ran for 57050 frames Cell Events: 3569 mean_circ: 0.931785
Ran for 57550 frames Cell Events: 3603 mean_circ: 0.931754
Ran for 59050 frames Cell Events: 3663 mean_circ: 0.931767
Ran for 60050 frames Cell Events: 3722 mean_circ: 0.931798
Ran for 62050 frames Cell Events: 3830 mean_circ: 0.931784
Ran for 63550 frames Cell Events: 3902 mean_circ: 0.931772
Ran for 64050 frames Cell Events: 3942 mean_circ: 0.931854
Ran for 64550 frames Cell Events: 3975 mean_circ: 0.931809
Ran for 65050 frames Cell Events: 3999 mean_circ: 0.931824
Ran for 66050 frames Cell Events: 4056 mean_circ: 0.931938
Ran for 66550 frames Cell Events: 4099 mean_circ: 0.931975
Ran for 67050 frames Cell Events: 4129 mean_circ: 0.932009
Ran for 70550 frames Cell Events: 4390 mean_circ: 0.931908
Ran for 71050 frames Cell Events: 4413 mean_circ: 0.931828
Ran for 71550 frames Cell Events: 4431 mean_circ: 0.931885
Ran for 73550 frames Cell Events: 4573 mean_circ: 0.931819
Ran for 74550 frames Cell Events: 4628 mean_circ: 0.931964
Ran for 75050 frames Cell Events: 4650 mean_circ: 0.931982
Ran for 75550 frames Cell Events: 4681 mean_circ: 0.932082
Ran for 77050 frames Cell Events: 4786 mean_circ: 0.932181
Ran for 78050 frames Cell Events: 4852 mean_circ: 0.932121
Ran for 78550 frames Cell Events: 4890 mean_circ: 0.932121
Ran for 79050 frames Cell Events: 4935 mean_circ: 0.932162
Ran for 81550 frames Cell Events: 5101 mean_circ: 0.931926
Ran for 82050 frames Cell Events: 5129 mean_circ: 0.931859
Ran for 82550 frames Cell Events: 5161 mean_circ: 0.93182
Ran for 83550 frames Cell Events: 5194 mean_circ: 0.931884
Ran for 87050 frames Cell Events: 5436 mean_circ: 0.931986
Ran for 88050 frames Cell Events: 5522 mean_circ: 0.932009
Ran for 89550 frames Cell Events: 5607 mean_circ: 0.932063
Ran for 90050 frames Cell Events: 5635 mean_circ: 0.932077
Ran for 90550 frames Cell Events: 5663 mean_circ: 0.932058
Ran for 92050 frames Cell Events: 5761 mean_circ: 0.932086
Ran for 92550 frames Cell Events: 5793 mean_circ: 0.932076
Ran for 93550 frames Cell Events: 5860 mean_circ: 0.931996
Ran for 94050 frames Cell Events: 5896 mean_circ: 0.931941
Ran for 95050 frames Cell Events: 5952 mean_circ: 0.931894
Ran for 96550 frames Cell Events: 6053 mean_circ: 0.93179
Ran for 98050 frames Cell Events: 6149 mean_circ: 0.931872
Ran for 98550 frames Cell Events: 6167 mean_circ: 0.931805
Ran for 99050 frames Cell Events: 6200 mean_circ: 0.931739
Ran for 100050 frames Cell Events: 6269 mean_circ: 0.931705
Ran for 101550 frames Cell Events: 6381 mean_circ: 0.931635
Ran for 102050 frames Cell Events: 6416 mean_circ: 0.931623
Ran for 102550 frames Cell Events: 6445 mean_circ: 0.931582
Ran for 106550 frames Cell Events: 6680 mean_circ: 0.931521
Ran for 107550 frames Cell Events: 6749 mean_circ: 0.93162
Ran for 108550 frames Cell Events: 6812 mean_circ: 0.931627
Ran for 109550 frames Cell Events: 6869 mean_circ: 0.93159
Ran for 110050 frames Cell Events: 6889 mean_circ: 0.931652
Ran for 110550 frames Cell Events: 6922 mean_circ: 0.931723
Ran for 111050 frames Cell Events: 6963 mean_circ: 0.93171
Ran for 119050 frames Cell Events: 7465 mean_circ: 0.931574
Ran for 120050 frames Cell Events: 7519 mean_circ: 0.931508
Ran for 121550 frames Cell Events: 7633 mean_circ: 0.931449
Ran for 125050 frames Cell Events: 7891 mean_circ: 0.93145
Ran for 125550 frames Cell Events: 7919 mean_circ: 0.93145
Ran for 126550 frames Cell Events: 7989 mean_circ: 0.93145
Ran for 127050 frames Cell Events: 8024 mean_circ: 0.931489
Ran for 128550 frames Cell Events: 8108 mean_circ: 0.93151



End this measurment..



Stopping time: 1.76509e+009
****************************************
Measurement finished

Mean Circularitiy: 0.931484
Mean Area: 629.86
Mean FL-1 intensity: 5149.39
Mean FL-2 intensity: 6140.64
Mean FL-3 intensity: 0

frames processed: 125423
Total Time: 64568.5ms
Number of Runs (new bg img) 0
Frames collected: 129042
Skipped frames: 133805
Rescued Frames: 4596881
Samples DAQ: 32261156
Samples written: 64520918
Samples per Channel: 32260459
FPS: 1998.53

Samples per second: 499631
Expected samples/s: 500000

Events: 8142
Events per second: 126.099
jumped back (PMT): 25
#Frames read, frequency
# 0 frames :199697
# 1 frames :38527
# 2 frames :2228
# 3 frames :866
# 4 frames :473
# 5 frames :234
# 6 frames :1606
# 7 frames :975
# 8 frames :836
# 9 frames :571
# 10 frames :448
# 11 frames :306
# 12 frames :235
# 13 frames :200
# 14 frames :204
# 15 frames :203
# 16 frames :145
# 17 frames :134
# 18 frames :100
# 19 frames :75
# 20 frames :80
# 21 frames :71
# 22 frames :62
# 23 frames :50
# 24 frames :57
# 25 frames :40
# 26 frames :43
# 27 frames :41
# 28 frames :34
# 29 frames :34
# 30 frames :34
# 31 frames :17
# 32 frames :25
# 33 frames :12
# 34 frames :19
# 35 frames :18
# 36 frames :14
# 37 frames :13
# 38 frames :12
# 39 frames :20
# 40 frames :13
# 41 frames :7
# 42 frames :10
# 43 frames :8
# 44 frames :5
# 45 frames :3
# 46 frames :5
# 47 frames :6
# 48 frames :4
# 49 frames :4
# 50 frames :6
# 51 frames :3
# 52 frames :3
# 53 frames :4
# 54 frames :1
# 55 frames :1
# 56 frames :2
# 57 frames :3
# 58 frames :1
# 60 frames :2
# 61 frames :1
# 62 frames :1
# 63 frames :3
# 64 frames :2
# 65 frames :2
# 67 frames :2
# 70 frames :1
# 71 frames :1
# 72 frames :2
# 73 frames :1
# 74 frames :2
# 78 frames :1
# 84 frames :2
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.2459
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0