Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_12.56.55 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "1226f56b19ab2c045f5929d3606461d2",
"name": "M1_20170604_1937_dsRNA_H4_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "10:57:14"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8065 (max 8066) in '8065'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.2974
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.76844e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 49 mean_circ: 0.935381
Ran for 1550 frames Cell Events: 64 mean_circ: 0.935973
Ran for 2550 frames Cell Events: 124 mean_circ: 0.938717
Ran for 4550 frames Cell Events: 246 mean_circ: 0.938621
Ran for 5050 frames Cell Events: 267 mean_circ: 0.938879
Ran for 6550 frames Cell Events: 363 mean_circ: 0.936237
Ran for 7050 frames Cell Events: 394 mean_circ: 0.935954
Ran for 7550 frames Cell Events: 416 mean_circ: 0.936544
Ran for 9050 frames Cell Events: 499 mean_circ: 0.93575
Ran for 10050 frames Cell Events: 553 mean_circ: 0.936327
Ran for 11550 frames Cell Events: 645 mean_circ: 0.937058
Ran for 12550 frames Cell Events: 721 mean_circ: 0.936213
Ran for 13050 frames Cell Events: 765 mean_circ: 0.935884
Ran for 13550 frames Cell Events: 784 mean_circ: 0.935781
Ran for 16550 frames Cell Events: 996 mean_circ: 0.936214
Ran for 17050 frames Cell Events: 1020 mean_circ: 0.936518
Ran for 22550 frames Cell Events: 1376 mean_circ: 0.936533
Ran for 24050 frames Cell Events: 1482 mean_circ: 0.936742
Ran for 25550 frames Cell Events: 1577 mean_circ: 0.936743
Ran for 26550 frames Cell Events: 1641 mean_circ: 0.936921
Ran for 27550 frames Cell Events: 1691 mean_circ: 0.937015
Ran for 28050 frames Cell Events: 1720 mean_circ: 0.937301
Ran for 29050 frames Cell Events: 1790 mean_circ: 0.937393
Ran for 29550 frames Cell Events: 1817 mean_circ: 0.937151
Ran for 30550 frames Cell Events: 1877 mean_circ: 0.937311
Ran for 36050 frames Cell Events: 2194 mean_circ: 0.936759
Ran for 36550 frames Cell Events: 2225 mean_circ: 0.93677
Ran for 37050 frames Cell Events: 2246 mean_circ: 0.936876
Ran for 39050 frames Cell Events: 2371 mean_circ: 0.937203
Ran for 40550 frames Cell Events: 2470 mean_circ: 0.937352
Ran for 42050 frames Cell Events: 2576 mean_circ: 0.937512
Ran for 42550 frames Cell Events: 2598 mean_circ: 0.937602
Ran for 43050 frames Cell Events: 2625 mean_circ: 0.937571
Ran for 43550 frames Cell Events: 2648 mean_circ: 0.937552
Ran for 44550 frames Cell Events: 2698 mean_circ: 0.93761
Ran for 45050 frames Cell Events: 2729 mean_circ: 0.937572
Ran for 45550 frames Cell Events: 2744 mean_circ: 0.937505
Ran for 47550 frames Cell Events: 2862 mean_circ: 0.937362
Ran for 48050 frames Cell Events: 2885 mean_circ: 0.937357
Ran for 48550 frames Cell Events: 2915 mean_circ: 0.937388
Ran for 49050 frames Cell Events: 2952 mean_circ: 0.937495
Ran for 49550 frames Cell Events: 2980 mean_circ: 0.93744
Ran for 50050 frames Cell Events: 3009 mean_circ: 0.937428
Ran for 50550 frames Cell Events: 3031 mean_circ: 0.937445
Ran for 52550 frames Cell Events: 3161 mean_circ: 0.937489
Ran for 53050 frames Cell Events: 3195 mean_circ: 0.937286
Ran for 53550 frames Cell Events: 3225 mean_circ: 0.937353
Ran for 55050 frames Cell Events: 3324 mean_circ: 0.937394
Ran for 57550 frames Cell Events: 3490 mean_circ: 0.937326
Ran for 58550 frames Cell Events: 3542 mean_circ: 0.937231
Ran for 59550 frames Cell Events: 3612 mean_circ: 0.937224
Ran for 60050 frames Cell Events: 3633 mean_circ: 0.937319
Ran for 62050 frames Cell Events: 3762 mean_circ: 0.937333
Ran for 62550 frames Cell Events: 3787 mean_circ: 0.937411
Ran for 63550 frames Cell Events: 3864 mean_circ: 0.937186
Ran for 65050 frames Cell Events: 3956 mean_circ: 0.937307
Ran for 66050 frames Cell Events: 4013 mean_circ: 0.937342
Ran for 67550 frames Cell Events: 4098 mean_circ: 0.93727
Ran for 69050 frames Cell Events: 4226 mean_circ: 0.937311
Ran for 69550 frames Cell Events: 4254 mean_circ: 0.937335
Ran for 72050 frames Cell Events: 4455 mean_circ: 0.937519
Ran for 73050 frames Cell Events: 4512 mean_circ: 0.937546
Ran for 74050 frames Cell Events: 4586 mean_circ: 0.937613
Ran for 74550 frames Cell Events: 4615 mean_circ: 0.937598
Ran for 75550 frames Cell Events: 4674 mean_circ: 0.937634
Ran for 76050 frames Cell Events: 4706 mean_circ: 0.93765
Ran for 78550 frames Cell Events: 4909 mean_circ: 0.937733
Ran for 79050 frames Cell Events: 4949 mean_circ: 0.937733
Ran for 79550 frames Cell Events: 4980 mean_circ: 0.937758
Ran for 80050 frames Cell Events: 5000 mean_circ: 0.937787
Ran for 80550 frames Cell Events: 5040 mean_circ: 0.937825
Ran for 81050 frames Cell Events: 5076 mean_circ: 0.937825
Ran for 82550 frames Cell Events: 5168 mean_circ: 0.937669
Ran for 83050 frames Cell Events: 5207 mean_circ: 0.937643
Ran for 84050 frames Cell Events: 5271 mean_circ: 0.937588
Ran for 84550 frames Cell Events: 5303 mean_circ: 0.937554
Ran for 86050 frames Cell Events: 5387 mean_circ: 0.937396
Ran for 87050 frames Cell Events: 5447 mean_circ: 0.937404
Ran for 87550 frames Cell Events: 5477 mean_circ: 0.937366
Ran for 88050 frames Cell Events: 5503 mean_circ: 0.937328
Ran for 90550 frames Cell Events: 5685 mean_circ: 0.937528
Ran for 93050 frames Cell Events: 5854 mean_circ: 0.937644
Ran for 94550 frames Cell Events: 5940 mean_circ: 0.937663
Ran for 95050 frames Cell Events: 5964 mean_circ: 0.937609
Ran for 96050 frames Cell Events: 6029 mean_circ: 0.937581
Ran for 97050 frames Cell Events: 6073 mean_circ: 0.9376
Ran for 97550 frames Cell Events: 6097 mean_circ: 0.93758
Ran for 98050 frames Cell Events: 6123 mean_circ: 0.93758
Ran for 99050 frames Cell Events: 6191 mean_circ: 0.937604
Ran for 100050 frames Cell Events: 6252 mean_circ: 0.937657
Ran for 101550 frames Cell Events: 6355 mean_circ: 0.937643
Ran for 102050 frames Cell Events: 6377 mean_circ: 0.937652
Ran for 103550 frames Cell Events: 6474 mean_circ: 0.937714
Ran for 104050 frames Cell Events: 6491 mean_circ: 0.937701
Ran for 104550 frames Cell Events: 6536 mean_circ: 0.937726
Ran for 105050 frames Cell Events: 6575 mean_circ: 0.937697
Ran for 106550 frames Cell Events: 6652 mean_circ: 0.937629
Ran for 109050 frames Cell Events: 6783 mean_circ: 0.937585
Ran for 109550 frames Cell Events: 6803 mean_circ: 0.937583
Ran for 111050 frames Cell Events: 6876 mean_circ: 0.937614
Ran for 111550 frames Cell Events: 6904 mean_circ: 0.937638
Ran for 112050 frames Cell Events: 6927 mean_circ: 0.93764
Ran for 112550 frames Cell Events: 6949 mean_circ: 0.937603
Ran for 115050 frames Cell Events: 7121 mean_circ: 0.937481
Ran for 115550 frames Cell Events: 7143 mean_circ: 0.937509
Ran for 120050 frames Cell Events: 7433 mean_circ: 0.93734
Ran for 122050 frames Cell Events: 7533 mean_circ: 0.937294
Ran for 123050 frames Cell Events: 7599 mean_circ: 0.937284
Ran for 123550 frames Cell Events: 7627 mean_circ: 0.937318
Ran for 124050 frames Cell Events: 7661 mean_circ: 0.937344
Ran for 126050 frames Cell Events: 7809 mean_circ: 0.93737
Ran for 126550 frames Cell Events: 7844 mean_circ: 0.93737
Ran for 128550 frames Cell Events: 7965 mean_circ: 0.937412
Ran for 129550 frames Cell Events: 8034 mean_circ: 0.937388



End this measurment..



Stopping time: 1.7685e+009
****************************************
Measurement finished

Mean Circularitiy: 0.937401
Mean Area: 590.045
Mean FL-1 intensity: 3298.99
Mean FL-2 intensity: 4067.19
Mean FL-3 intensity: 0

frames processed: 127591
Total Time: 65101.9ms
Number of Runs (new bg img) 0
Frames collected: 130011
Skipped frames: 154789
Rescued Frames: 5509698
Samples DAQ: 32503747
Samples written: 65005480
Samples per Channel: 32502740
FPS: 1997.04

Samples per second: 499259
Expected samples/s: 500000

Events: 8066
Events per second: 123.898
jumped back (PMT): 21
#Frames read, frequency
# 0 frames :206131
# 1 frames :41573
# 2 frames :2515
# 3 frames :910
# 4 frames :507
# 5 frames :230
# 6 frames :1536
# 7 frames :1250
# 8 frames :892
# 9 frames :584
# 10 frames :437
# 11 frames :306
# 12 frames :244
# 13 frames :188
# 14 frames :224
# 15 frames :190
# 16 frames :162
# 17 frames :129
# 18 frames :110
# 19 frames :74
# 20 frames :70
# 21 frames :66
# 22 frames :67
# 23 frames :50
# 24 frames :60
# 25 frames :37
# 26 frames :34
# 27 frames :19
# 28 frames :26
# 29 frames :20
# 30 frames :23
# 31 frames :25
# 32 frames :21
# 33 frames :21
# 34 frames :14
# 35 frames :10
# 36 frames :13
# 37 frames :8
# 38 frames :10
# 39 frames :12
# 40 frames :4
# 41 frames :11
# 42 frames :3
# 43 frames :4
# 44 frames :4
# 45 frames :3
# 47 frames :1
# 48 frames :2
# 49 frames :4
# 50 frames :3
# 51 frames :3
# 52 frames :1
# 53 frames :2
# 54 frames :1
# 55 frames :1
# 56 frames :4
# 57 frames :2
# 58 frames :1
# 59 frames :2
# 61 frames :2
# 75 frames :1
# 77 frames :1
# 80 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.2974
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0