Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_13.05.52 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "87fa9bd388be9bec5eb02673e057680f",
"name": "M1_20170605_1352_I1_fast.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "11:06:12"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8304 (max 8305) in '8304'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.4923
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.83418e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 54 mean_circ: 0.941252
Ran for 1550 frames Cell Events: 83 mean_circ: 0.928779
Ran for 2050 frames Cell Events: 111 mean_circ: 0.931706
Ran for 2550 frames Cell Events: 133 mean_circ: 0.931545
Ran for 3550 frames Cell Events: 198 mean_circ: 0.931332
Ran for 4550 frames Cell Events: 266 mean_circ: 0.933272
Ran for 6050 frames Cell Events: 307 mean_circ: 0.932218
Ran for 7050 frames Cell Events: 362 mean_circ: 0.93336
Ran for 8550 frames Cell Events: 457 mean_circ: 0.932235
Ran for 9050 frames Cell Events: 479 mean_circ: 0.932305
Ran for 9550 frames Cell Events: 502 mean_circ: 0.931893
Ran for 10050 frames Cell Events: 527 mean_circ: 0.932034
Ran for 11050 frames Cell Events: 573 mean_circ: 0.932096
Ran for 11550 frames Cell Events: 613 mean_circ: 0.931859
Ran for 12050 frames Cell Events: 645 mean_circ: 0.931845
Ran for 12550 frames Cell Events: 667 mean_circ: 0.932252
Ran for 13550 frames Cell Events: 716 mean_circ: 0.932866
Ran for 14050 frames Cell Events: 745 mean_circ: 0.933245
Ran for 16550 frames Cell Events: 858 mean_circ: 0.93286
Ran for 18050 frames Cell Events: 938 mean_circ: 0.93283
Ran for 18550 frames Cell Events: 965 mean_circ: 0.932899
Ran for 19050 frames Cell Events: 984 mean_circ: 0.933169
Ran for 20050 frames Cell Events: 1048 mean_circ: 0.93296
Ran for 22550 frames Cell Events: 1179 mean_circ: 0.931791
Ran for 23050 frames Cell Events: 1207 mean_circ: 0.93193
Ran for 23550 frames Cell Events: 1237 mean_circ: 0.931909
Ran for 24050 frames Cell Events: 1269 mean_circ: 0.931765
Ran for 26550 frames Cell Events: 1425 mean_circ: 0.931398
Ran for 31550 frames Cell Events: 1696 mean_circ: 0.93202
Ran for 32550 frames Cell Events: 1755 mean_circ: 0.932126
Ran for 33550 frames Cell Events: 1810 mean_circ: 0.932194
Ran for 35050 frames Cell Events: 1911 mean_circ: 0.931848
Ran for 36050 frames Cell Events: 1940 mean_circ: 0.931515
Ran for 37550 frames Cell Events: 1984 mean_circ: 0.931582
Ran for 38050 frames Cell Events: 2007 mean_circ: 0.931601
Ran for 39050 frames Cell Events: 2075 mean_circ: 0.931549
Ran for 40050 frames Cell Events: 2122 mean_circ: 0.931483
Ran for 40550 frames Cell Events: 2156 mean_circ: 0.931452
Ran for 41050 frames Cell Events: 2176 mean_circ: 0.931524
Ran for 41550 frames Cell Events: 2203 mean_circ: 0.93151
Ran for 42050 frames Cell Events: 2218 mean_circ: 0.931562
Ran for 43050 frames Cell Events: 2278 mean_circ: 0.931181
Ran for 43550 frames Cell Events: 2302 mean_circ: 0.931163
Ran for 44050 frames Cell Events: 2334 mean_circ: 0.931173
Ran for 45050 frames Cell Events: 2380 mean_circ: 0.931238
Ran for 45550 frames Cell Events: 2407 mean_circ: 0.931435
Ran for 46050 frames Cell Events: 2432 mean_circ: 0.931545
Ran for 46550 frames Cell Events: 2454 mean_circ: 0.931611
Ran for 48550 frames Cell Events: 2554 mean_circ: 0.93145
Ran for 49550 frames Cell Events: 2609 mean_circ: 0.931497
Ran for 51050 frames Cell Events: 2697 mean_circ: 0.931452
Ran for 51550 frames Cell Events: 2725 mean_circ: 0.93144
Ran for 52050 frames Cell Events: 2750 mean_circ: 0.931291
Ran for 53050 frames Cell Events: 2808 mean_circ: 0.931257
Ran for 53550 frames Cell Events: 2834 mean_circ: 0.93127
Ran for 54550 frames Cell Events: 2891 mean_circ: 0.931458
Ran for 56050 frames Cell Events: 2961 mean_circ: 0.931415
Ran for 59550 frames Cell Events: 3136 mean_circ: 0.93146
Ran for 60550 frames Cell Events: 3203 mean_circ: 0.931191
Ran for 61050 frames Cell Events: 3224 mean_circ: 0.931302
Ran for 63050 frames Cell Events: 3303 mean_circ: 0.93143
Ran for 64050 frames Cell Events: 3359 mean_circ: 0.931407
Ran for 64550 frames Cell Events: 3389 mean_circ: 0.931324
Ran for 66050 frames Cell Events: 3471 mean_circ: 0.931302
Ran for 66550 frames Cell Events: 3495 mean_circ: 0.931247
Ran for 67050 frames Cell Events: 3519 mean_circ: 0.931247
Ran for 68050 frames Cell Events: 3566 mean_circ: 0.931296
Ran for 69550 frames Cell Events: 3651 mean_circ: 0.93127
Ran for 70050 frames Cell Events: 3677 mean_circ: 0.931195
Ran for 70550 frames Cell Events: 3692 mean_circ: 0.931195
Ran for 74050 frames Cell Events: 3892 mean_circ: 0.931399
Ran for 74550 frames Cell Events: 3916 mean_circ: 0.931425
Ran for 75050 frames Cell Events: 3935 mean_circ: 0.931472
Ran for 75550 frames Cell Events: 3959 mean_circ: 0.931413
Ran for 76550 frames Cell Events: 4008 mean_circ: 0.931494
Ran for 77050 frames Cell Events: 4028 mean_circ: 0.931565
Ran for 78050 frames Cell Events: 4077 mean_circ: 0.93164
Ran for 78550 frames Cell Events: 4094 mean_circ: 0.931632
Ran for 79050 frames Cell Events: 4116 mean_circ: 0.931688
Ran for 80050 frames Cell Events: 4167 mean_circ: 0.931722
Ran for 80550 frames Cell Events: 4191 mean_circ: 0.931715
Ran for 82050 frames Cell Events: 4272 mean_circ: 0.931762
Ran for 83550 frames Cell Events: 4345 mean_circ: 0.931853
Ran for 85050 frames Cell Events: 4426 mean_circ: 0.931874
Ran for 87050 frames Cell Events: 4522 mean_circ: 0.932126
Ran for 88050 frames Cell Events: 4565 mean_circ: 0.932158
Ran for 88550 frames Cell Events: 4588 mean_circ: 0.932083
Ran for 89550 frames Cell Events: 4639 mean_circ: 0.932155
Ran for 90050 frames Cell Events: 4655 mean_circ: 0.932144
Ran for 90550 frames Cell Events: 4687 mean_circ: 0.932136
Ran for 91550 frames Cell Events: 4742 mean_circ: 0.93214
Ran for 92050 frames Cell Events: 4755 mean_circ: 0.932126
Ran for 93050 frames Cell Events: 4803 mean_circ: 0.9321
Ran for 94050 frames Cell Events: 4848 mean_circ: 0.932256
Ran for 94550 frames Cell Events: 4878 mean_circ: 0.932243
Ran for 95550 frames Cell Events: 4932 mean_circ: 0.93226
Ran for 96050 frames Cell Events: 4953 mean_circ: 0.932326
Ran for 96550 frames Cell Events: 4978 mean_circ: 0.932298
Ran for 97050 frames Cell Events: 5009 mean_circ: 0.932257
Ran for 97550 frames Cell Events: 5028 mean_circ: 0.932231
Ran for 98050 frames Cell Events: 5055 mean_circ: 0.932271
Ran for 99550 frames Cell Events: 5134 mean_circ: 0.932293
Ran for 100050 frames Cell Events: 5158 mean_circ: 0.932304
Ran for 100550 frames Cell Events: 5183 mean_circ: 0.932363
Ran for 101050 frames Cell Events: 5206 mean_circ: 0.932305
Ran for 101550 frames Cell Events: 5241 mean_circ: 0.932305
Ran for 102050 frames Cell Events: 5264 mean_circ: 0.932409
Ran for 102550 frames Cell Events: 5294 mean_circ: 0.932455
Ran for 103050 frames Cell Events: 5320 mean_circ: 0.932488
Ran for 103550 frames Cell Events: 5340 mean_circ: 0.932514
Ran for 104050 frames Cell Events: 5357 mean_circ: 0.932488
Ran for 104550 frames Cell Events: 5379 mean_circ: 0.932563
Ran for 105050 frames Cell Events: 5393 mean_circ: 0.932602
Ran for 106050 frames Cell Events: 5442 mean_circ: 0.932634
Ran for 107550 frames Cell Events: 5510 mean_circ: 0.932729
Ran for 109050 frames Cell Events: 5581 mean_circ: 0.932785
Ran for 110050 frames Cell Events: 5641 mean_circ: 0.932817
Ran for 111050 frames Cell Events: 5686 mean_circ: 0.932795
Ran for 112050 frames Cell Events: 5726 mean_circ: 0.9327
Ran for 112550 frames Cell Events: 5754 mean_circ: 0.93272
Ran for 113550 frames Cell Events: 5816 mean_circ: 0.932811
Ran for 115550 frames Cell Events: 5910 mean_circ: 0.932771
Ran for 116550 frames Cell Events: 5961 mean_circ: 0.932769
Ran for 117550 frames Cell Events: 6016 mean_circ: 0.932805
Ran for 119550 frames Cell Events: 6121 mean_circ: 0.932754
Ran for 120050 frames Cell Events: 6142 mean_circ: 0.932714
Ran for 121050 frames Cell Events: 6203 mean_circ: 0.932605
Ran for 121550 frames Cell Events: 6229 mean_circ: 0.932644
Ran for 122050 frames Cell Events: 6260 mean_circ: 0.93265
Ran for 122550 frames Cell Events: 6292 mean_circ: 0.932606
Ran for 123550 frames Cell Events: 6355 mean_circ: 0.932588
Ran for 124050 frames Cell Events: 6388 mean_circ: 0.93257
Ran for 124550 frames Cell Events: 6423 mean_circ: 0.932563
Ran for 125050 frames Cell Events: 6441 mean_circ: 0.932559
Ran for 125550 frames Cell Events: 6461 mean_circ: 0.932578
Ran for 128050 frames Cell Events: 6602 mean_circ: 0.932581
Ran for 128550 frames Cell Events: 6621 mean_circ: 0.932598
Ran for 129550 frames Cell Events: 6670 mean_circ: 0.932645
Ran for 130550 frames Cell Events: 6725 mean_circ: 0.93258
Ran for 131550 frames Cell Events: 6784 mean_circ: 0.932618
Ran for 132050 frames Cell Events: 6802 mean_circ: 0.932548
Ran for 132550 frames Cell Events: 6825 mean_circ: 0.932586
Ran for 133050 frames Cell Events: 6849 mean_circ: 0.932582
Ran for 133550 frames Cell Events: 6869 mean_circ: 0.932574
Ran for 134050 frames Cell Events: 6887 mean_circ: 0.932565
Ran for 135050 frames Cell Events: 6957 mean_circ: 0.932552
Ran for 136050 frames Cell Events: 7010 mean_circ: 0.932603
Ran for 138050 frames Cell Events: 7103 mean_circ: 0.932606
Ran for 138550 frames Cell Events: 7130 mean_circ: 0.932609
Ran for 139050 frames Cell Events: 7148 mean_circ: 0.932628
Ran for 140050 frames Cell Events: 7203 mean_circ: 0.932634
Ran for 140550 frames Cell Events: 7222 mean_circ: 0.932668
Ran for 141050 frames Cell Events: 7239 mean_circ: 0.932642
Ran for 142050 frames Cell Events: 7300 mean_circ: 0.932594
Ran for 142550 frames Cell Events: 7330 mean_circ: 0.932615
Ran for 143550 frames Cell Events: 7375 mean_circ: 0.932618
Ran for 144050 frames Cell Events: 7407 mean_circ: 0.932616
Ran for 145050 frames Cell Events: 7456 mean_circ: 0.932671
Ran for 145550 frames Cell Events: 7474 mean_circ: 0.932708
Ran for 146550 frames Cell Events: 7528 mean_circ: 0.93272
Ran for 147050 frames Cell Events: 7545 mean_circ: 0.932708
Ran for 147550 frames Cell Events: 7573 mean_circ: 0.932701
Ran for 148050 frames Cell Events: 7597 mean_circ: 0.932707
Ran for 149050 frames Cell Events: 7636 mean_circ: 0.932746
Ran for 149550 frames Cell Events: 7651 mean_circ: 0.932752
Ran for 151050 frames Cell Events: 7717 mean_circ: 0.932838
Ran for 151550 frames Cell Events: 7742 mean_circ: 0.932845
Ran for 154550 frames Cell Events: 7877 mean_circ: 0.932803
Ran for 156550 frames Cell Events: 7956 mean_circ: 0.932781
Ran for 157550 frames Cell Events: 8004 mean_circ: 0.932793
Ran for 158050 frames Cell Events: 8025 mean_circ: 0.932797
Ran for 159550 frames Cell Events: 8109 mean_circ: 0.932751
Ran for 160050 frames Cell Events: 8139 mean_circ: 0.932784
Ran for 161050 frames Cell Events: 8184 mean_circ: 0.932708
Ran for 162050 frames Cell Events: 8218 mean_circ: 0.932703
Ran for 163550 frames Cell Events: 8286 mean_circ: 0.932668



End this measurment..



Stopping time: 1.83427e+009
****************************************
Measurement finished

Mean Circularitiy: 0.932679
Mean Area: 594.877
Mean FL-1 intensity: 3531.02
Mean FL-2 intensity: 4645.99
Mean FL-3 intensity: 0

frames processed: 161797
Total Time: 82043.1ms
Number of Runs (new bg img) 0
Frames collected: 163943
Skipped frames: 4510
Rescued Frames: 374206
Samples DAQ: 40985830
Samples written: 81971570
Samples per Channel: 40985785
FPS: 1998.26

Samples per second: 499564
Expected samples/s: 500000

Events: 8305
Events per second: 101.227
jumped back (PMT): 15
#Frames read, frequency
# 0 frames :251713
# 1 frames :62214
# 2 frames :3456
# 3 frames :1309
# 4 frames :719
# 5 frames :287
# 6 frames :1750
# 7 frames :1265
# 8 frames :1031
# 9 frames :674
# 10 frames :884
# 11 frames :449
# 12 frames :360
# 13 frames :221
# 14 frames :244
# 15 frames :195
# 16 frames :182
# 17 frames :169
# 18 frames :105
# 19 frames :92
# 20 frames :76
# 21 frames :72
# 22 frames :56
# 23 frames :44
# 24 frames :47
# 25 frames :24
# 26 frames :23
# 27 frames :31
# 28 frames :18
# 29 frames :18
# 30 frames :13
# 31 frames :15
# 32 frames :13
# 33 frames :11
# 34 frames :6
# 35 frames :9
# 36 frames :4
# 37 frames :4
# 38 frames :6
# 39 frames :8
# 40 frames :2
# 41 frames :5
# 42 frames :9
# 43 frames :4
# 44 frames :5
# 46 frames :2
# 47 frames :5
# 48 frames :1
# 49 frames :2
# 50 frames :1
# 52 frames :4
# 53 frames :1
# 58 frames :1
# 60 frames :2
# 62 frames :1
# 69 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.4923
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0