Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_13.06.45 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "c990d78c905717454e2aa0138b6eaf82",
"name": "M1_20170605_1404_dsRNA_i7_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "11:07:08"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 9111 (max 9112) in '9111'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.4994
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.83489e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 30 mean_circ: 0.944322
Ran for 2550 frames Cell Events: 138 mean_circ: 0.944937
Ran for 3550 frames Cell Events: 187 mean_circ: 0.942789
Ran for 4050 frames Cell Events: 208 mean_circ: 0.943379
Ran for 6550 frames Cell Events: 328 mean_circ: 0.943281
Ran for 7050 frames Cell Events: 355 mean_circ: 0.942631
Ran for 7550 frames Cell Events: 384 mean_circ: 0.942865
Ran for 8050 frames Cell Events: 403 mean_circ: 0.943094
Ran for 10050 frames Cell Events: 481 mean_circ: 0.942794
Ran for 10550 frames Cell Events: 517 mean_circ: 0.942237
Ran for 11550 frames Cell Events: 563 mean_circ: 0.941801
Ran for 12550 frames Cell Events: 614 mean_circ: 0.941741
Ran for 13550 frames Cell Events: 682 mean_circ: 0.941391
Ran for 14050 frames Cell Events: 699 mean_circ: 0.941105
Ran for 14550 frames Cell Events: 724 mean_circ: 0.940813
Ran for 15050 frames Cell Events: 741 mean_circ: 0.940774
Ran for 15550 frames Cell Events: 763 mean_circ: 0.940788
Ran for 16050 frames Cell Events: 787 mean_circ: 0.941079
Ran for 18050 frames Cell Events: 906 mean_circ: 0.941148
Ran for 20550 frames Cell Events: 1034 mean_circ: 0.940682
Ran for 21550 frames Cell Events: 1079 mean_circ: 0.940806
Ran for 22550 frames Cell Events: 1116 mean_circ: 0.941061
Ran for 23550 frames Cell Events: 1158 mean_circ: 0.941014
Ran for 24550 frames Cell Events: 1216 mean_circ: 0.940909
Ran for 25050 frames Cell Events: 1249 mean_circ: 0.940773
Ran for 25550 frames Cell Events: 1290 mean_circ: 0.940742
Ran for 26550 frames Cell Events: 1355 mean_circ: 0.940319
Ran for 27050 frames Cell Events: 1380 mean_circ: 0.939563
Ran for 29050 frames Cell Events: 1502 mean_circ: 0.939342
Ran for 33050 frames Cell Events: 1727 mean_circ: 0.938983
Ran for 34050 frames Cell Events: 1771 mean_circ: 0.938676
Ran for 35050 frames Cell Events: 1813 mean_circ: 0.938805
Ran for 37550 frames Cell Events: 1947 mean_circ: 0.938566
Ran for 38050 frames Cell Events: 1969 mean_circ: 0.938504
Ran for 38550 frames Cell Events: 1999 mean_circ: 0.938449
Ran for 39050 frames Cell Events: 2027 mean_circ: 0.938475
Ran for 39550 frames Cell Events: 2043 mean_circ: 0.938544
Ran for 40550 frames Cell Events: 2086 mean_circ: 0.938621
Ran for 41050 frames Cell Events: 2107 mean_circ: 0.938666
Ran for 42550 frames Cell Events: 2181 mean_circ: 0.938509
Ran for 44050 frames Cell Events: 2277 mean_circ: 0.937897
Ran for 44550 frames Cell Events: 2303 mean_circ: 0.937997
Ran for 46050 frames Cell Events: 2378 mean_circ: 0.938005
Ran for 48550 frames Cell Events: 2495 mean_circ: 0.937854
Ran for 49050 frames Cell Events: 2514 mean_circ: 0.937751
Ran for 53550 frames Cell Events: 2747 mean_circ: 0.938046
Ran for 55550 frames Cell Events: 2853 mean_circ: 0.938119
Ran for 56050 frames Cell Events: 2883 mean_circ: 0.93804
Ran for 56550 frames Cell Events: 2908 mean_circ: 0.938074
Ran for 58550 frames Cell Events: 3012 mean_circ: 0.938127
Ran for 59050 frames Cell Events: 3039 mean_circ: 0.938158
Ran for 59550 frames Cell Events: 3065 mean_circ: 0.938155
Ran for 61050 frames Cell Events: 3137 mean_circ: 0.937983
Ran for 61550 frames Cell Events: 3175 mean_circ: 0.937916
Ran for 62050 frames Cell Events: 3199 mean_circ: 0.938123
Ran for 64050 frames Cell Events: 3295 mean_circ: 0.938137
Ran for 64550 frames Cell Events: 3316 mean_circ: 0.938097
Ran for 65050 frames Cell Events: 3348 mean_circ: 0.938063
Ran for 65550 frames Cell Events: 3368 mean_circ: 0.938113
Ran for 67550 frames Cell Events: 3484 mean_circ: 0.938231
Ran for 68550 frames Cell Events: 3535 mean_circ: 0.938082
Ran for 72550 frames Cell Events: 3738 mean_circ: 0.937779
Ran for 73050 frames Cell Events: 3757 mean_circ: 0.93768
Ran for 74550 frames Cell Events: 3812 mean_circ: 0.937652
Ran for 75050 frames Cell Events: 3841 mean_circ: 0.937666
Ran for 76550 frames Cell Events: 3932 mean_circ: 0.937546
Ran for 77050 frames Cell Events: 3966 mean_circ: 0.937542
Ran for 77550 frames Cell Events: 3988 mean_circ: 0.93751
Ran for 79050 frames Cell Events: 4121 mean_circ: 0.937516
Ran for 79550 frames Cell Events: 4158 mean_circ: 0.937345
Ran for 81550 frames Cell Events: 4255 mean_circ: 0.937367
Ran for 82050 frames Cell Events: 4279 mean_circ: 0.937344
Ran for 84050 frames Cell Events: 4392 mean_circ: 0.937159
Ran for 84550 frames Cell Events: 4416 mean_circ: 0.937157
Ran for 85050 frames Cell Events: 4434 mean_circ: 0.937169
Ran for 85550 frames Cell Events: 4461 mean_circ: 0.937181
Ran for 86550 frames Cell Events: 4480 mean_circ: 0.937243
Ran for 88550 frames Cell Events: 4601 mean_circ: 0.937218
Ran for 90050 frames Cell Events: 4687 mean_circ: 0.937186
Ran for 90550 frames Cell Events: 4710 mean_circ: 0.937129
Ran for 92050 frames Cell Events: 4794 mean_circ: 0.937138
Ran for 93550 frames Cell Events: 4843 mean_circ: 0.937074
Ran for 96050 frames Cell Events: 4972 mean_circ: 0.936871
Ran for 97050 frames Cell Events: 5019 mean_circ: 0.936845
Ran for 98050 frames Cell Events: 5064 mean_circ: 0.936701
Ran for 98550 frames Cell Events: 5093 mean_circ: 0.936689
Ran for 99050 frames Cell Events: 5113 mean_circ: 0.936637
Ran for 99550 frames Cell Events: 5129 mean_circ: 0.936634
Ran for 100550 frames Cell Events: 5175 mean_circ: 0.936626
Ran for 101050 frames Cell Events: 5196 mean_circ: 0.936614
Ran for 101550 frames Cell Events: 5227 mean_circ: 0.936587
Ran for 102550 frames Cell Events: 5282 mean_circ: 0.936682
Ran for 103550 frames Cell Events: 5330 mean_circ: 0.936654
Ran for 104050 frames Cell Events: 5355 mean_circ: 0.936569
Ran for 105050 frames Cell Events: 5403 mean_circ: 0.936585
Ran for 106050 frames Cell Events: 5480 mean_circ: 0.93651
Ran for 107550 frames Cell Events: 5531 mean_circ: 0.936442
Ran for 108550 frames Cell Events: 5588 mean_circ: 0.936387
Ran for 109050 frames Cell Events: 5620 mean_circ: 0.936325
Ran for 109550 frames Cell Events: 5643 mean_circ: 0.936363
Ran for 110550 frames Cell Events: 5705 mean_circ: 0.936447
Ran for 112050 frames Cell Events: 5797 mean_circ: 0.936339
Ran for 113050 frames Cell Events: 5855 mean_circ: 0.936349
Ran for 115550 frames Cell Events: 6004 mean_circ: 0.936318
Ran for 116050 frames Cell Events: 6028 mean_circ: 0.936305
Ran for 116550 frames Cell Events: 6060 mean_circ: 0.936301
Ran for 117050 frames Cell Events: 6091 mean_circ: 0.936247
Ran for 117550 frames Cell Events: 6120 mean_circ: 0.936217
Ran for 118550 frames Cell Events: 6179 mean_circ: 0.936187
Ran for 119050 frames Cell Events: 6203 mean_circ: 0.936186
Ran for 121050 frames Cell Events: 6313 mean_circ: 0.936165
Ran for 121550 frames Cell Events: 6344 mean_circ: 0.93622
Ran for 122050 frames Cell Events: 6367 mean_circ: 0.93622
Ran for 123550 frames Cell Events: 6458 mean_circ: 0.936259
Ran for 124050 frames Cell Events: 6486 mean_circ: 0.936307
Ran for 126550 frames Cell Events: 6627 mean_circ: 0.936429
Ran for 128050 frames Cell Events: 6717 mean_circ: 0.936354
Ran for 128550 frames Cell Events: 6748 mean_circ: 0.936336
Ran for 130050 frames Cell Events: 6843 mean_circ: 0.936361
Ran for 131050 frames Cell Events: 6900 mean_circ: 0.936387
Ran for 132050 frames Cell Events: 6956 mean_circ: 0.936331
Ran for 132550 frames Cell Events: 6976 mean_circ: 0.936369
Ran for 133550 frames Cell Events: 7016 mean_circ: 0.936415
Ran for 134550 frames Cell Events: 7068 mean_circ: 0.936365
Ran for 135050 frames Cell Events: 7096 mean_circ: 0.936395
Ran for 136550 frames Cell Events: 7181 mean_circ: 0.936336
Ran for 138050 frames Cell Events: 7275 mean_circ: 0.936381
Ran for 139050 frames Cell Events: 7324 mean_circ: 0.936393
Ran for 139550 frames Cell Events: 7344 mean_circ: 0.9364
Ran for 140050 frames Cell Events: 7374 mean_circ: 0.93637
Ran for 140550 frames Cell Events: 7399 mean_circ: 0.936293
Ran for 142550 frames Cell Events: 7483 mean_circ: 0.936283
Ran for 143050 frames Cell Events: 7509 mean_circ: 0.936299
Ran for 143550 frames Cell Events: 7544 mean_circ: 0.936301
Ran for 144050 frames Cell Events: 7558 mean_circ: 0.936312
Ran for 144550 frames Cell Events: 7588 mean_circ: 0.936342
Ran for 148550 frames Cell Events: 7822 mean_circ: 0.936221
Ran for 149550 frames Cell Events: 7869 mean_circ: 0.936191
Ran for 151550 frames Cell Events: 7976 mean_circ: 0.936207
Ran for 152050 frames Cell Events: 7991 mean_circ: 0.936205
Ran for 153050 frames Cell Events: 8046 mean_circ: 0.936219
Ran for 153550 frames Cell Events: 8076 mean_circ: 0.936204
Ran for 154050 frames Cell Events: 8097 mean_circ: 0.936224
Ran for 156550 frames Cell Events: 8241 mean_circ: 0.936261
Ran for 157050 frames Cell Events: 8260 mean_circ: 0.936237
Ran for 158550 frames Cell Events: 8324 mean_circ: 0.936277
Ran for 159050 frames Cell Events: 8353 mean_circ: 0.936297
Ran for 160550 frames Cell Events: 8441 mean_circ: 0.936302
Ran for 161050 frames Cell Events: 8464 mean_circ: 0.93628
Ran for 162550 frames Cell Events: 8544 mean_circ: 0.936285
Ran for 164050 frames Cell Events: 8613 mean_circ: 0.936268
Ran for 165550 frames Cell Events: 8706 mean_circ: 0.936185
Ran for 166550 frames Cell Events: 8773 mean_circ: 0.936156
Ran for 167550 frames Cell Events: 8838 mean_circ: 0.936173
Ran for 169050 frames Cell Events: 8915 mean_circ: 0.936145
Ran for 170550 frames Cell Events: 9001 mean_circ: 0.936177
Ran for 171550 frames Cell Events: 9043 mean_circ: 0.936147



End this measurment..



Stopping time: 1.83498e+009
****************************************
Measurement finished

Mean Circularitiy: 0.936163
Mean Area: 578.579
Mean FL-1 intensity: 3439.64
Mean FL-2 intensity: 4505.91
Mean FL-3 intensity: 0

frames processed: 169734
Total Time: 86341.5ms
Number of Runs (new bg img) 0
Frames collected: 172523
Skipped frames: 8682
Rescued Frames: 546523
Samples DAQ: 43138041
Samples written: 86261604
Samples per Channel: 43130802
FPS: 1998.15

Samples per second: 499537
Expected samples/s: 500000

Events: 9112
Events per second: 105.534
jumped back (PMT): 25
#Frames read, frequency
# 0 frames :268495
# 1 frames :61708
# 2 frames :3431
# 3 frames :1324
# 4 frames :791
# 5 frames :377
# 6 frames :1657
# 7 frames :1341
# 8 frames :1004
# 9 frames :974
# 10 frames :819
# 11 frames :478
# 12 frames :386
# 13 frames :226
# 14 frames :188
# 15 frames :203
# 16 frames :209
# 17 frames :138
# 18 frames :140
# 19 frames :102
# 20 frames :72
# 21 frames :92
# 22 frames :60
# 23 frames :47
# 24 frames :49
# 25 frames :53
# 26 frames :43
# 27 frames :42
# 28 frames :29
# 29 frames :27
# 30 frames :26
# 31 frames :21
# 32 frames :22
# 33 frames :14
# 34 frames :12
# 35 frames :11
# 36 frames :10
# 37 frames :9
# 38 frames :6
# 39 frames :7
# 40 frames :2
# 41 frames :4
# 42 frames :8
# 43 frames :7
# 44 frames :9
# 45 frames :3
# 46 frames :7
# 47 frames :3
# 48 frames :2
# 49 frames :3
# 50 frames :7
# 51 frames :1
# 52 frames :2
# 53 frames :1
# 54 frames :3
# 56 frames :2
# 57 frames :2
# 58 frames :2
# 59 frames :1
# 60 frames :1
# 62 frames :1
# 67 frames :1
# 77 frames :1
# 85 frames :1
# 87 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.4994
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0