Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_13.07.44 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "70de8b7f830e2ab75e183d2bf151e03e",
"name": "M1_20170605_1415_dsRNA_J5_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "11:08:04"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8040 (max 8041) in '8040'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.4912
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.83557e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 31 mean_circ: 0.94472
Ran for 1550 frames Cell Events: 45 mean_circ: 0.949756
Ran for 2050 frames Cell Events: 62 mean_circ: 0.948272
Ran for 3550 frames Cell Events: 87 mean_circ: 0.946583
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Ran for 4550 frames Cell Events: 124 mean_circ: 0.947385
Ran for 5050 frames Cell Events: 139 mean_circ: 0.947469
Ran for 6550 frames Cell Events: 185 mean_circ: 0.94618
Ran for 7550 frames Cell Events: 216 mean_circ: 0.94506
Ran for 8550 frames Cell Events: 246 mean_circ: 0.945051
Ran for 9050 frames Cell Events: 257 mean_circ: 0.944862
Ran for 9550 frames Cell Events: 272 mean_circ: 0.945329
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Ran for 11050 frames Cell Events: 329 mean_circ: 0.945064
Ran for 11550 frames Cell Events: 336 mean_circ: 0.944899
Ran for 12050 frames Cell Events: 352 mean_circ: 0.944879
Ran for 12550 frames Cell Events: 373 mean_circ: 0.945115
Ran for 13050 frames Cell Events: 395 mean_circ: 0.945503
Ran for 13550 frames Cell Events: 412 mean_circ: 0.945748
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Ran for 14550 frames Cell Events: 441 mean_circ: 0.945901
Ran for 15050 frames Cell Events: 455 mean_circ: 0.946103
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Ran for 22050 frames Cell Events: 699 mean_circ: 0.944727
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Ran for 60550 frames Cell Events: 1922 mean_circ: 0.942976
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Ran for 62050 frames Cell Events: 1975 mean_circ: 0.942859
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Ran for 64550 frames Cell Events: 2083 mean_circ: 0.942752
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Ran for 73550 frames Cell Events: 2358 mean_circ: 0.94238
Ran for 74050 frames Cell Events: 2368 mean_circ: 0.94239
Ran for 74550 frames Cell Events: 2376 mean_circ: 0.942414
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Ran for 81050 frames Cell Events: 2583 mean_circ: 0.942347
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Ran for 83050 frames Cell Events: 2645 mean_circ: 0.9424
Ran for 84550 frames Cell Events: 2707 mean_circ: 0.942203
Ran for 85550 frames Cell Events: 2740 mean_circ: 0.942138
Ran for 86050 frames Cell Events: 2765 mean_circ: 0.942136
Ran for 86550 frames Cell Events: 2780 mean_circ: 0.942254
Ran for 87050 frames Cell Events: 2794 mean_circ: 0.942219
Ran for 87550 frames Cell Events: 2811 mean_circ: 0.942253
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Ran for 90550 frames Cell Events: 2911 mean_circ: 0.942311
Ran for 91050 frames Cell Events: 2935 mean_circ: 0.942174
Ran for 91550 frames Cell Events: 2958 mean_circ: 0.942086
Ran for 92050 frames Cell Events: 2975 mean_circ: 0.942067
Ran for 92550 frames Cell Events: 2987 mean_circ: 0.942059
Ran for 93550 frames Cell Events: 3019 mean_circ: 0.942056
Ran for 94550 frames Cell Events: 3053 mean_circ: 0.942108
Ran for 95050 frames Cell Events: 3070 mean_circ: 0.942194
Ran for 98550 frames Cell Events: 3231 mean_circ: 0.942139
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Ran for 100050 frames Cell Events: 3282 mean_circ: 0.942217
Ran for 101050 frames Cell Events: 3322 mean_circ: 0.942166
Ran for 101550 frames Cell Events: 3334 mean_circ: 0.942242
Ran for 102050 frames Cell Events: 3350 mean_circ: 0.942264
Ran for 102550 frames Cell Events: 3361 mean_circ: 0.942253
Ran for 103550 frames Cell Events: 3402 mean_circ: 0.942332
Ran for 105550 frames Cell Events: 3475 mean_circ: 0.942321
Ran for 106050 frames Cell Events: 3501 mean_circ: 0.942299
Ran for 107050 frames Cell Events: 3544 mean_circ: 0.942319
Ran for 107550 frames Cell Events: 3560 mean_circ: 0.94219
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Ran for 110050 frames Cell Events: 3662 mean_circ: 0.942201
Ran for 110550 frames Cell Events: 3675 mean_circ: 0.942246
Ran for 111550 frames Cell Events: 3719 mean_circ: 0.942104
Ran for 112050 frames Cell Events: 3747 mean_circ: 0.942128
Ran for 112550 frames Cell Events: 3761 mean_circ: 0.942118
Ran for 113050 frames Cell Events: 3778 mean_circ: 0.942118
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Ran for 115050 frames Cell Events: 3840 mean_circ: 0.942139
Ran for 116050 frames Cell Events: 3880 mean_circ: 0.942136
Ran for 116550 frames Cell Events: 3900 mean_circ: 0.94221
Ran for 117050 frames Cell Events: 3916 mean_circ: 0.942206
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Ran for 119050 frames Cell Events: 3974 mean_circ: 0.94213
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Ran for 120050 frames Cell Events: 4017 mean_circ: 0.942245
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Ran for 121050 frames Cell Events: 4053 mean_circ: 0.942273
Ran for 121550 frames Cell Events: 4070 mean_circ: 0.942291
Ran for 122050 frames Cell Events: 4094 mean_circ: 0.942342
Ran for 122550 frames Cell Events: 4115 mean_circ: 0.942257
Ran for 123050 frames Cell Events: 4130 mean_circ: 0.942282
Ran for 124550 frames Cell Events: 4193 mean_circ: 0.942237
Ran for 125050 frames Cell Events: 4206 mean_circ: 0.942192
Ran for 125550 frames Cell Events: 4225 mean_circ: 0.942118
Ran for 127050 frames Cell Events: 4281 mean_circ: 0.942122
Ran for 127550 frames Cell Events: 4302 mean_circ: 0.942122
Ran for 128050 frames Cell Events: 4323 mean_circ: 0.942115
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Ran for 130050 frames Cell Events: 4399 mean_circ: 0.941957
Ran for 132050 frames Cell Events: 4472 mean_circ: 0.941901
Ran for 132550 frames Cell Events: 4506 mean_circ: 0.941916
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Ran for 141550 frames Cell Events: 4786 mean_circ: 0.941848
Ran for 142550 frames Cell Events: 4816 mean_circ: 0.941871
Ran for 143550 frames Cell Events: 4849 mean_circ: 0.941913
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Ran for 146550 frames Cell Events: 4964 mean_circ: 0.94197
Ran for 147050 frames Cell Events: 4973 mean_circ: 0.942002
Ran for 147550 frames Cell Events: 4995 mean_circ: 0.94201
Ran for 148050 frames Cell Events: 5023 mean_circ: 0.941991
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Ran for 167050 frames Cell Events: 5666 mean_circ: 0.941721
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Ran for 171050 frames Cell Events: 5781 mean_circ: 0.941743
Ran for 171550 frames Cell Events: 5793 mean_circ: 0.941729
Ran for 173050 frames Cell Events: 5835 mean_circ: 0.941742
Ran for 173550 frames Cell Events: 5846 mean_circ: 0.94173
Ran for 174050 frames Cell Events: 5859 mean_circ: 0.941715
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Ran for 182050 frames Cell Events: 6150 mean_circ: 0.941703
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Ran for 183550 frames Cell Events: 6197 mean_circ: 0.941664
Ran for 184050 frames Cell Events: 6212 mean_circ: 0.941692
Ran for 184550 frames Cell Events: 6228 mean_circ: 0.941668
Ran for 185050 frames Cell Events: 6246 mean_circ: 0.941662
Ran for 186550 frames Cell Events: 6295 mean_circ: 0.941637
Ran for 187050 frames Cell Events: 6305 mean_circ: 0.941658
Ran for 188050 frames Cell Events: 6348 mean_circ: 0.941661
Ran for 188550 frames Cell Events: 6369 mean_circ: 0.941672
Ran for 189050 frames Cell Events: 6389 mean_circ: 0.94168
Ran for 189550 frames Cell Events: 6410 mean_circ: 0.941633
Ran for 190050 frames Cell Events: 6421 mean_circ: 0.941659
Ran for 190550 frames Cell Events: 6444 mean_circ: 0.941658
Ran for 191550 frames Cell Events: 6482 mean_circ: 0.941651
Ran for 192050 frames Cell Events: 6505 mean_circ: 0.941638
Ran for 193050 frames Cell Events: 6535 mean_circ: 0.941646
Ran for 193550 frames Cell Events: 6548 mean_circ: 0.941674
Ran for 194050 frames Cell Events: 6564 mean_circ: 0.941715
Ran for 194550 frames Cell Events: 6589 mean_circ: 0.941745
Ran for 195050 frames Cell Events: 6605 mean_circ: 0.941722
Ran for 195550 frames Cell Events: 6622 mean_circ: 0.941702
Ran for 196550 frames Cell Events: 6646 mean_circ: 0.94168
Ran for 197550 frames Cell Events: 6690 mean_circ: 0.941678
Ran for 198050 frames Cell Events: 6704 mean_circ: 0.941679
Ran for 199050 frames Cell Events: 6752 mean_circ: 0.94165
Ran for 199550 frames Cell Events: 6772 mean_circ: 0.941625
Ran for 200050 frames Cell Events: 6786 mean_circ: 0.941629
Ran for 200550 frames Cell Events: 6803 mean_circ: 0.941658
Ran for 201050 frames Cell Events: 6817 mean_circ: 0.941651
Ran for 201550 frames Cell Events: 6831 mean_circ: 0.941665
Ran for 202050 frames Cell Events: 6859 mean_circ: 0.94169
Ran for 203050 frames Cell Events: 6905 mean_circ: 0.941686
Ran for 203550 frames Cell Events: 6923 mean_circ: 0.941685
Ran for 204550 frames Cell Events: 6966 mean_circ: 0.941671
Ran for 205050 frames Cell Events: 6984 mean_circ: 0.941651
Ran for 205550 frames Cell Events: 7006 mean_circ: 0.941654
Ran for 206050 frames Cell Events: 7028 mean_circ: 0.941635
Ran for 207050 frames Cell Events: 7064 mean_circ: 0.941642
Ran for 207550 frames Cell Events: 7084 mean_circ: 0.941634
Ran for 208050 frames Cell Events: 7111 mean_circ: 0.941605
Ran for 208550 frames Cell Events: 7128 mean_circ: 0.941564
Ran for 209050 frames Cell Events: 7148 mean_circ: 0.941526
Ran for 210050 frames Cell Events: 7187 mean_circ: 0.941504
Ran for 210550 frames Cell Events: 7202 mean_circ: 0.941515
Ran for 211050 frames Cell Events: 7223 mean_circ: 0.941533
Ran for 211550 frames Cell Events: 7244 mean_circ: 0.941542
Ran for 212550 frames Cell Events: 7280 mean_circ: 0.941592
Ran for 213050 frames Cell Events: 7295 mean_circ: 0.94161
Ran for 213550 frames Cell Events: 7313 mean_circ: 0.941621
Ran for 214050 frames Cell Events: 7327 mean_circ: 0.941651
Ran for 214550 frames Cell Events: 7334 mean_circ: 0.941636
Ran for 215050 frames Cell Events: 7360 mean_circ: 0.941619
Ran for 215550 frames Cell Events: 7373 mean_circ: 0.941627
Ran for 216050 frames Cell Events: 7386 mean_circ: 0.94155
Ran for 216550 frames Cell Events: 7412 mean_circ: 0.941548
Ran for 217050 frames Cell Events: 7433 mean_circ: 0.941523
Ran for 218050 frames Cell Events: 7471 mean_circ: 0.941551
Ran for 219050 frames Cell Events: 7514 mean_circ: 0.941552
Ran for 220050 frames Cell Events: 7566 mean_circ: 0.941513
Ran for 221550 frames Cell Events: 7619 mean_circ: 0.94153
Ran for 222550 frames Cell Events: 7646 mean_circ: 0.941487
Ran for 223050 frames Cell Events: 7667 mean_circ: 0.941479
Ran for 223550 frames Cell Events: 7691 mean_circ: 0.941452
Ran for 224050 frames Cell Events: 7707 mean_circ: 0.941479
Ran for 224550 frames Cell Events: 7726 mean_circ: 0.941472
Ran for 225050 frames Cell Events: 7740 mean_circ: 0.941472
Ran for 226050 frames Cell Events: 7785 mean_circ: 0.941389
Ran for 227550 frames Cell Events: 7839 mean_circ: 0.941391
Ran for 228050 frames Cell Events: 7854 mean_circ: 0.941395
Ran for 228550 frames Cell Events: 7866 mean_circ: 0.941406
Ran for 230050 frames Cell Events: 7920 mean_circ: 0.941418
Ran for 230550 frames Cell Events: 7941 mean_circ: 0.941433
Ran for 231050 frames Cell Events: 7960 mean_circ: 0.941431
Ran for 231550 frames Cell Events: 7975 mean_circ: 0.941414
Ran for 232050 frames Cell Events: 7993 mean_circ: 0.94143
Ran for 232550 frames Cell Events: 8010 mean_circ: 0.941422
Ran for 233050 frames Cell Events: 8032 mean_circ: 0.941418



End this measurment..



Stopping time: 1.83569e+009
****************************************
Measurement finished

Mean Circularitiy: 0.941424
Mean Area: 588.599
Mean FL-1 intensity: 3169.53
Mean FL-2 intensity: 4075.08
Mean FL-3 intensity: 0

frames processed: 229368
Total Time: 116676ms
Number of Runs (new bg img) 0
Frames collected: 233223
Skipped frames: 13921
Rescued Frames: 733979
Samples DAQ: 58306116
Samples written: 116611280
Samples per Channel: 58305640
FPS: 1998.9

Samples per second: 499723
Expected samples/s: 500000

Events: 8041
Events per second: 68.9174
jumped back (PMT): 20
#Frames read, frequency
# 0 frames :313410
# 1 frames :136589
# 2 frames :4955
# 3 frames :1501
# 4 frames :552
# 5 frames :206
# 6 frames :3795
# 7 frames :1573
# 8 frames :1272
# 9 frames :504
# 10 frames :563
# 11 frames :339
# 12 frames :359
# 13 frames :166
# 14 frames :186
# 15 frames :113
# 16 frames :79
# 17 frames :63
# 18 frames :40
# 19 frames :23
# 20 frames :27
# 21 frames :18
# 22 frames :20
# 23 frames :6
# 24 frames :5
# 25 frames :6
# 26 frames :8
# 27 frames :6
# 28 frames :3
# 29 frames :7
# 30 frames :4
# 31 frames :5
# 32 frames :2
# 33 frames :2
# 34 frames :2
# 36 frames :1
# 41 frames :1
# 46 frames :1
# 51 frames :1
# 54 frames :1
# 83 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.4912
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0