Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_13.13.55 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "06255950fef067af372419daeda68ec6",
"name": "M1_20170605_1537_dsRNA_J1_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "11:14:15"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8038 (max 8040) in '8038'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.4086
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.84049e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 23 mean_circ: 0.916644
Ran for 2050 frames Cell Events: 73 mean_circ: 0.942372
Ran for 2550 frames Cell Events: 96 mean_circ: 0.938692
Ran for 3550 frames Cell Events: 135 mean_circ: 0.942454
Ran for 4050 frames Cell Events: 163 mean_circ: 0.943917
Ran for 5050 frames Cell Events: 197 mean_circ: 0.945976
Ran for 6050 frames Cell Events: 237 mean_circ: 0.945517
Ran for 6550 frames Cell Events: 249 mean_circ: 0.946112
Ran for 7050 frames Cell Events: 260 mean_circ: 0.946052
Ran for 8550 frames Cell Events: 320 mean_circ: 0.947636
Ran for 11050 frames Cell Events: 440 mean_circ: 0.947094
Ran for 11550 frames Cell Events: 458 mean_circ: 0.946352
Ran for 14050 frames Cell Events: 531 mean_circ: 0.945603
Ran for 15550 frames Cell Events: 592 mean_circ: 0.94482
Ran for 16550 frames Cell Events: 633 mean_circ: 0.944938
Ran for 18550 frames Cell Events: 688 mean_circ: 0.945078
Ran for 19050 frames Cell Events: 701 mean_circ: 0.944948
Ran for 19550 frames Cell Events: 726 mean_circ: 0.944265
Ran for 20050 frames Cell Events: 736 mean_circ: 0.944267
Ran for 21050 frames Cell Events: 777 mean_circ: 0.944661
Ran for 22050 frames Cell Events: 814 mean_circ: 0.944316
Ran for 22550 frames Cell Events: 835 mean_circ: 0.944068
Ran for 23550 frames Cell Events: 877 mean_circ: 0.944446
Ran for 24050 frames Cell Events: 902 mean_circ: 0.944377
Ran for 25050 frames Cell Events: 934 mean_circ: 0.944617
Ran for 28550 frames Cell Events: 1102 mean_circ: 0.944274
Ran for 30550 frames Cell Events: 1159 mean_circ: 0.944244
Ran for 32050 frames Cell Events: 1212 mean_circ: 0.944763
Ran for 32550 frames Cell Events: 1227 mean_circ: 0.944889
Ran for 33550 frames Cell Events: 1270 mean_circ: 0.944577
Ran for 35050 frames Cell Events: 1336 mean_circ: 0.944717
Ran for 36050 frames Cell Events: 1383 mean_circ: 0.944767
Ran for 36550 frames Cell Events: 1394 mean_circ: 0.944672
Ran for 38550 frames Cell Events: 1494 mean_circ: 0.944884
Ran for 39050 frames Cell Events: 1517 mean_circ: 0.944938
Ran for 39550 frames Cell Events: 1534 mean_circ: 0.945072
Ran for 40550 frames Cell Events: 1572 mean_circ: 0.944914
Ran for 44550 frames Cell Events: 1761 mean_circ: 0.944601
Ran for 45050 frames Cell Events: 1779 mean_circ: 0.944759
Ran for 45550 frames Cell Events: 1794 mean_circ: 0.944828
Ran for 46050 frames Cell Events: 1816 mean_circ: 0.94496
Ran for 47050 frames Cell Events: 1857 mean_circ: 0.945039
Ran for 48050 frames Cell Events: 1910 mean_circ: 0.945118
Ran for 48550 frames Cell Events: 1938 mean_circ: 0.945141
Ran for 50550 frames Cell Events: 2053 mean_circ: 0.94461
Ran for 51550 frames Cell Events: 2098 mean_circ: 0.944502
Ran for 52050 frames Cell Events: 2121 mean_circ: 0.944505
Ran for 52550 frames Cell Events: 2142 mean_circ: 0.944478
Ran for 53050 frames Cell Events: 2163 mean_circ: 0.944375
Ran for 54050 frames Cell Events: 2223 mean_circ: 0.94425
Ran for 54550 frames Cell Events: 2246 mean_circ: 0.944252
Ran for 55050 frames Cell Events: 2276 mean_circ: 0.944182
Ran for 56050 frames Cell Events: 2336 mean_circ: 0.943958
Ran for 57050 frames Cell Events: 2376 mean_circ: 0.944081
Ran for 58550 frames Cell Events: 2451 mean_circ: 0.944124
Ran for 59550 frames Cell Events: 2489 mean_circ: 0.944039
Ran for 61550 frames Cell Events: 2585 mean_circ: 0.943849
Ran for 62050 frames Cell Events: 2604 mean_circ: 0.943889
Ran for 63550 frames Cell Events: 2671 mean_circ: 0.943918
Ran for 64050 frames Cell Events: 2691 mean_circ: 0.943938
Ran for 64550 frames Cell Events: 2711 mean_circ: 0.943863
Ran for 65050 frames Cell Events: 2728 mean_circ: 0.943913
Ran for 67550 frames Cell Events: 2851 mean_circ: 0.943862
Ran for 68550 frames Cell Events: 2882 mean_circ: 0.943829
Ran for 69050 frames Cell Events: 2907 mean_circ: 0.943895
Ran for 69550 frames Cell Events: 2922 mean_circ: 0.943801
Ran for 70050 frames Cell Events: 2939 mean_circ: 0.94382
Ran for 70550 frames Cell Events: 2962 mean_circ: 0.943734
Ran for 71550 frames Cell Events: 3025 mean_circ: 0.943812
Ran for 72050 frames Cell Events: 3049 mean_circ: 0.94379
Ran for 72550 frames Cell Events: 3071 mean_circ: 0.943796
Ran for 74050 frames Cell Events: 3153 mean_circ: 0.943704
Ran for 74550 frames Cell Events: 3174 mean_circ: 0.943595
Ran for 75050 frames Cell Events: 3198 mean_circ: 0.943597
Ran for 76050 frames Cell Events: 3241 mean_circ: 0.943647
Ran for 77050 frames Cell Events: 3300 mean_circ: 0.943714
Ran for 78550 frames Cell Events: 3368 mean_circ: 0.94372
Ran for 79050 frames Cell Events: 3393 mean_circ: 0.943671
Ran for 79550 frames Cell Events: 3416 mean_circ: 0.943534
Ran for 85050 frames Cell Events: 3719 mean_circ: 0.943663
Ran for 86050 frames Cell Events: 3776 mean_circ: 0.943653
Ran for 86550 frames Cell Events: 3797 mean_circ: 0.94359
Ran for 89050 frames Cell Events: 3911 mean_circ: 0.943564
Ran for 89550 frames Cell Events: 3936 mean_circ: 0.943518
Ran for 90050 frames Cell Events: 3953 mean_circ: 0.943501
Ran for 90550 frames Cell Events: 3965 mean_circ: 0.943549
Ran for 91050 frames Cell Events: 3986 mean_circ: 0.943542
Ran for 92050 frames Cell Events: 4041 mean_circ: 0.943499
Ran for 92550 frames Cell Events: 4065 mean_circ: 0.943525
Ran for 93050 frames Cell Events: 4089 mean_circ: 0.943468
Ran for 94050 frames Cell Events: 4124 mean_circ: 0.943482
Ran for 94550 frames Cell Events: 4159 mean_circ: 0.943466
Ran for 95550 frames Cell Events: 4214 mean_circ: 0.943416
Ran for 96550 frames Cell Events: 4267 mean_circ: 0.943307
Ran for 97050 frames Cell Events: 4281 mean_circ: 0.943345
Ran for 97550 frames Cell Events: 4310 mean_circ: 0.943323
Ran for 99550 frames Cell Events: 4407 mean_circ: 0.943355
Ran for 100050 frames Cell Events: 4429 mean_circ: 0.943382
Ran for 101050 frames Cell Events: 4467 mean_circ: 0.943339
Ran for 101550 frames Cell Events: 4480 mean_circ: 0.94338
Ran for 102550 frames Cell Events: 4522 mean_circ: 0.94335
Ran for 103050 frames Cell Events: 4545 mean_circ: 0.943376
Ran for 104550 frames Cell Events: 4599 mean_circ: 0.943442
Ran for 107550 frames Cell Events: 4756 mean_circ: 0.94356
Ran for 109050 frames Cell Events: 4823 mean_circ: 0.94344
Ran for 109550 frames Cell Events: 4842 mean_circ: 0.943528
Ran for 110050 frames Cell Events: 4871 mean_circ: 0.943497
Ran for 110550 frames Cell Events: 4898 mean_circ: 0.943549
Ran for 111050 frames Cell Events: 4922 mean_circ: 0.943585
Ran for 111550 frames Cell Events: 4940 mean_circ: 0.943599
Ran for 112050 frames Cell Events: 4962 mean_circ: 0.943628
Ran for 112550 frames Cell Events: 4980 mean_circ: 0.943663
Ran for 113550 frames Cell Events: 5019 mean_circ: 0.943714
Ran for 114050 frames Cell Events: 5050 mean_circ: 0.943774
Ran for 115050 frames Cell Events: 5095 mean_circ: 0.943644
Ran for 116550 frames Cell Events: 5181 mean_circ: 0.943717
Ran for 117550 frames Cell Events: 5228 mean_circ: 0.943703
Ran for 122550 frames Cell Events: 5449 mean_circ: 0.943823
Ran for 123550 frames Cell Events: 5487 mean_circ: 0.943862
Ran for 124050 frames Cell Events: 5509 mean_circ: 0.943881
Ran for 124550 frames Cell Events: 5534 mean_circ: 0.943883
Ran for 125550 frames Cell Events: 5583 mean_circ: 0.943865
Ran for 126050 frames Cell Events: 5602 mean_circ: 0.943866
Ran for 127050 frames Cell Events: 5638 mean_circ: 0.943806
Ran for 127550 frames Cell Events: 5662 mean_circ: 0.943839
Ran for 128050 frames Cell Events: 5687 mean_circ: 0.943859
Ran for 128550 frames Cell Events: 5714 mean_circ: 0.943908
Ran for 130050 frames Cell Events: 5787 mean_circ: 0.943949
Ran for 131050 frames Cell Events: 5837 mean_circ: 0.944025
Ran for 131550 frames Cell Events: 5859 mean_circ: 0.944028
Ran for 133550 frames Cell Events: 5951 mean_circ: 0.944029
Ran for 134550 frames Cell Events: 6007 mean_circ: 0.943984
Ran for 135050 frames Cell Events: 6029 mean_circ: 0.943975
Ran for 135550 frames Cell Events: 6047 mean_circ: 0.943926
Ran for 136550 frames Cell Events: 6103 mean_circ: 0.943939
Ran for 137550 frames Cell Events: 6151 mean_circ: 0.943883
Ran for 138050 frames Cell Events: 6169 mean_circ: 0.94387
Ran for 139050 frames Cell Events: 6237 mean_circ: 0.943842
Ran for 139550 frames Cell Events: 6271 mean_circ: 0.943842
Ran for 140050 frames Cell Events: 6290 mean_circ: 0.943831
Ran for 140550 frames Cell Events: 6314 mean_circ: 0.943794
Ran for 142050 frames Cell Events: 6374 mean_circ: 0.943832
Ran for 143550 frames Cell Events: 6458 mean_circ: 0.943917
Ran for 145050 frames Cell Events: 6543 mean_circ: 0.943843
Ran for 145550 frames Cell Events: 6562 mean_circ: 0.943872
Ran for 147550 frames Cell Events: 6645 mean_circ: 0.943851
Ran for 149050 frames Cell Events: 6708 mean_circ: 0.9438
Ran for 150550 frames Cell Events: 6774 mean_circ: 0.943856
Ran for 151050 frames Cell Events: 6798 mean_circ: 0.943824
Ran for 151550 frames Cell Events: 6818 mean_circ: 0.943828
Ran for 152050 frames Cell Events: 6835 mean_circ: 0.943872
Ran for 152550 frames Cell Events: 6855 mean_circ: 0.943881
Ran for 153050 frames Cell Events: 6873 mean_circ: 0.943847
Ran for 155050 frames Cell Events: 6926 mean_circ: 0.943886
Ran for 156050 frames Cell Events: 6976 mean_circ: 0.943952
Ran for 156550 frames Cell Events: 6999 mean_circ: 0.943948
Ran for 157550 frames Cell Events: 7050 mean_circ: 0.944041
Ran for 158050 frames Cell Events: 7067 mean_circ: 0.944043
Ran for 158550 frames Cell Events: 7092 mean_circ: 0.944038
Ran for 159050 frames Cell Events: 7109 mean_circ: 0.944067
Ran for 160050 frames Cell Events: 7140 mean_circ: 0.944071
Ran for 160550 frames Cell Events: 7156 mean_circ: 0.944059
Ran for 161050 frames Cell Events: 7181 mean_circ: 0.94407
Ran for 162050 frames Cell Events: 7221 mean_circ: 0.944071
Ran for 162550 frames Cell Events: 7248 mean_circ: 0.944048
Ran for 164050 frames Cell Events: 7334 mean_circ: 0.944093
Ran for 164550 frames Cell Events: 7355 mean_circ: 0.944099
Ran for 165050 frames Cell Events: 7371 mean_circ: 0.944109
Ran for 166050 frames Cell Events: 7406 mean_circ: 0.944111
Ran for 167550 frames Cell Events: 7487 mean_circ: 0.9441
Ran for 168050 frames Cell Events: 7506 mean_circ: 0.94409
Ran for 169050 frames Cell Events: 7549 mean_circ: 0.94412
Ran for 169550 frames Cell Events: 7569 mean_circ: 0.94412
Ran for 170050 frames Cell Events: 7580 mean_circ: 0.944088
Ran for 171550 frames Cell Events: 7641 mean_circ: 0.944065
Ran for 172550 frames Cell Events: 7686 mean_circ: 0.944081
Ran for 173550 frames Cell Events: 7745 mean_circ: 0.944099
Ran for 175050 frames Cell Events: 7834 mean_circ: 0.944087
Ran for 177050 frames Cell Events: 7943 mean_circ: 0.944022



End this measurment..



Stopping time: 1.84058e+009
****************************************
Measurement finished

Mean Circularitiy: 0.944065
Mean Area: 603.759
Mean FL-1 intensity: 3364
Mean FL-2 intensity: 3893.52
Mean FL-3 intensity: 0

frames processed: 176120
Total Time: 89794.2ms
Number of Runs (new bg img) 0
Frames collected: 179370
Skipped frames: 26888
Rescued Frames: 1330525
Samples DAQ: 44842441
Samples written: 89684710
Samples per Channel: 44842355
FPS: 1997.57

Samples per second: 499390
Expected samples/s: 500000

Events: 8040
Events per second: 89.5381
jumped back (PMT): 3588
#Frames read, frequency
# 0 frames :270422
# 1 frames :73968
# 2 frames :3542
# 3 frames :1319
# 4 frames :783
# 5 frames :384
# 6 frames :1457
# 7 frames :1457
# 8 frames :1054
# 9 frames :682
# 10 frames :579
# 11 frames :427
# 12 frames :380
# 13 frames :280
# 14 frames :271
# 15 frames :230
# 16 frames :188
# 17 frames :154
# 18 frames :118
# 19 frames :97
# 20 frames :89
# 21 frames :66
# 22 frames :72
# 23 frames :55
# 24 frames :39
# 25 frames :42
# 26 frames :37
# 27 frames :40
# 28 frames :30
# 29 frames :18
# 30 frames :24
# 31 frames :17
# 32 frames :10
# 33 frames :11
# 34 frames :20
# 35 frames :12
# 36 frames :5
# 37 frames :11
# 38 frames :5
# 39 frames :7
# 40 frames :10
# 41 frames :6
# 42 frames :4
# 43 frames :5
# 44 frames :2
# 45 frames :2
# 46 frames :2
# 47 frames :1
# 48 frames :3
# 49 frames :1
# 51 frames :2
# 52 frames :4
# 53 frames :2
# 54 frames :3
# 55 frames :2
# 61 frames :1
# 63 frames :1
# 65 frames :1
# 68 frames :1
# 94 frames :1
# 96 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.4086
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0