Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_13.22.31 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "7fb74856faaa3a12e86e2983c9b0e5e1",
"name": "M1_20170605_1728_dsRNA_k5_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "11:22:51"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8035 (max 8036) in '8035'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.5901
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.84715e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 36 mean_circ: 0.930524
Ran for 2550 frames Cell Events: 171 mean_circ: 0.934715
Ran for 4550 frames Cell Events: 253 mean_circ: 0.935679
Ran for 5550 frames Cell Events: 316 mean_circ: 0.936559
Ran for 6050 frames Cell Events: 349 mean_circ: 0.936291
Ran for 7050 frames Cell Events: 394 mean_circ: 0.935187
Ran for 7550 frames Cell Events: 421 mean_circ: 0.935514
Ran for 8550 frames Cell Events: 470 mean_circ: 0.936124
Ran for 9550 frames Cell Events: 516 mean_circ: 0.935868
Ran for 10550 frames Cell Events: 573 mean_circ: 0.93561
Ran for 11050 frames Cell Events: 601 mean_circ: 0.935845
Ran for 11550 frames Cell Events: 627 mean_circ: 0.935848
Ran for 12050 frames Cell Events: 657 mean_circ: 0.93604
Ran for 14050 frames Cell Events: 781 mean_circ: 0.935463
Ran for 14550 frames Cell Events: 802 mean_circ: 0.93508
Ran for 15050 frames Cell Events: 822 mean_circ: 0.935169
Ran for 15550 frames Cell Events: 844 mean_circ: 0.935297
Ran for 17050 frames Cell Events: 940 mean_circ: 0.93606
Ran for 17550 frames Cell Events: 970 mean_circ: 0.936206
Ran for 18050 frames Cell Events: 1001 mean_circ: 0.935983
Ran for 18550 frames Cell Events: 1024 mean_circ: 0.935944
Ran for 19050 frames Cell Events: 1051 mean_circ: 0.93601
Ran for 19550 frames Cell Events: 1088 mean_circ: 0.936079
Ran for 21050 frames Cell Events: 1198 mean_circ: 0.936068
Ran for 22050 frames Cell Events: 1241 mean_circ: 0.936141
Ran for 23550 frames Cell Events: 1310 mean_circ: 0.935486
Ran for 24050 frames Cell Events: 1352 mean_circ: 0.935423
Ran for 24550 frames Cell Events: 1378 mean_circ: 0.935357
Ran for 25050 frames Cell Events: 1427 mean_circ: 0.935425
Ran for 26050 frames Cell Events: 1482 mean_circ: 0.935486
Ran for 26550 frames Cell Events: 1510 mean_circ: 0.935367
Ran for 27550 frames Cell Events: 1569 mean_circ: 0.935458
Ran for 28050 frames Cell Events: 1595 mean_circ: 0.93524
Ran for 28550 frames Cell Events: 1619 mean_circ: 0.93526
Ran for 29050 frames Cell Events: 1649 mean_circ: 0.935358
Ran for 29550 frames Cell Events: 1691 mean_circ: 0.93521
Ran for 30050 frames Cell Events: 1716 mean_circ: 0.93489
Ran for 31050 frames Cell Events: 1760 mean_circ: 0.934736
Ran for 31550 frames Cell Events: 1782 mean_circ: 0.934686
Ran for 32050 frames Cell Events: 1803 mean_circ: 0.934836
Ran for 32550 frames Cell Events: 1824 mean_circ: 0.934867
Ran for 33050 frames Cell Events: 1848 mean_circ: 0.934897
Ran for 33550 frames Cell Events: 1876 mean_circ: 0.934905
Ran for 34050 frames Cell Events: 1894 mean_circ: 0.934925
Ran for 35050 frames Cell Events: 1953 mean_circ: 0.934698
Ran for 35550 frames Cell Events: 1976 mean_circ: 0.934725
Ran for 36050 frames Cell Events: 2004 mean_circ: 0.934584
Ran for 36550 frames Cell Events: 2027 mean_circ: 0.934687
Ran for 37050 frames Cell Events: 2056 mean_circ: 0.934676
Ran for 38050 frames Cell Events: 2128 mean_circ: 0.934731
Ran for 38550 frames Cell Events: 2152 mean_circ: 0.934801
Ran for 39550 frames Cell Events: 2205 mean_circ: 0.934642
Ran for 40050 frames Cell Events: 2236 mean_circ: 0.934496
Ran for 40550 frames Cell Events: 2253 mean_circ: 0.934575
Ran for 41050 frames Cell Events: 2282 mean_circ: 0.934667
Ran for 41550 frames Cell Events: 2305 mean_circ: 0.934606
Ran for 42550 frames Cell Events: 2367 mean_circ: 0.934421
Ran for 43050 frames Cell Events: 2392 mean_circ: 0.934394
Ran for 43550 frames Cell Events: 2425 mean_circ: 0.934287
Ran for 44050 frames Cell Events: 2452 mean_circ: 0.934266
Ran for 44550 frames Cell Events: 2482 mean_circ: 0.93406
Ran for 45550 frames Cell Events: 2544 mean_circ: 0.934096
Ran for 47050 frames Cell Events: 2633 mean_circ: 0.933934
Ran for 48550 frames Cell Events: 2743 mean_circ: 0.933968
Ran for 49050 frames Cell Events: 2787 mean_circ: 0.93396
Ran for 50050 frames Cell Events: 2865 mean_circ: 0.934196
Ran for 50550 frames Cell Events: 2896 mean_circ: 0.934204
Ran for 51050 frames Cell Events: 2909 mean_circ: 0.934096
Ran for 51550 frames Cell Events: 2932 mean_circ: 0.934119
Ran for 52050 frames Cell Events: 2964 mean_circ: 0.934104
Ran for 52550 frames Cell Events: 2991 mean_circ: 0.934143
Ran for 53050 frames Cell Events: 3018 mean_circ: 0.934179
Ran for 53550 frames Cell Events: 3047 mean_circ: 0.934118
Ran for 54050 frames Cell Events: 3068 mean_circ: 0.93414
Ran for 54550 frames Cell Events: 3110 mean_circ: 0.93407
Ran for 55050 frames Cell Events: 3144 mean_circ: 0.933969
Ran for 56050 frames Cell Events: 3189 mean_circ: 0.934077
Ran for 56550 frames Cell Events: 3217 mean_circ: 0.934167
Ran for 57050 frames Cell Events: 3241 mean_circ: 0.934035
Ran for 58550 frames Cell Events: 3306 mean_circ: 0.933984
Ran for 62550 frames Cell Events: 3565 mean_circ: 0.934042
Ran for 63050 frames Cell Events: 3586 mean_circ: 0.933998
Ran for 64050 frames Cell Events: 3640 mean_circ: 0.933922
Ran for 64550 frames Cell Events: 3667 mean_circ: 0.933848
Ran for 65050 frames Cell Events: 3696 mean_circ: 0.933739
Ran for 65550 frames Cell Events: 3727 mean_circ: 0.933805
Ran for 66050 frames Cell Events: 3748 mean_circ: 0.933908
Ran for 67550 frames Cell Events: 3800 mean_circ: 0.93389
Ran for 68550 frames Cell Events: 3845 mean_circ: 0.933879
Ran for 70050 frames Cell Events: 3935 mean_circ: 0.933905
Ran for 70550 frames Cell Events: 3968 mean_circ: 0.933843
Ran for 72050 frames Cell Events: 4057 mean_circ: 0.933774
Ran for 72550 frames Cell Events: 4084 mean_circ: 0.933923
Ran for 73550 frames Cell Events: 4138 mean_circ: 0.933971
Ran for 74550 frames Cell Events: 4215 mean_circ: 0.934043
Ran for 76550 frames Cell Events: 4337 mean_circ: 0.9341
Ran for 77050 frames Cell Events: 4363 mean_circ: 0.934141
Ran for 78050 frames Cell Events: 4430 mean_circ: 0.934102
Ran for 78550 frames Cell Events: 4454 mean_circ: 0.934022
Ran for 79550 frames Cell Events: 4514 mean_circ: 0.933998
Ran for 81050 frames Cell Events: 4602 mean_circ: 0.934062
Ran for 81550 frames Cell Events: 4638 mean_circ: 0.934113
Ran for 82050 frames Cell Events: 4664 mean_circ: 0.934096
Ran for 82550 frames Cell Events: 4692 mean_circ: 0.934072
Ran for 83050 frames Cell Events: 4716 mean_circ: 0.934035
Ran for 83550 frames Cell Events: 4740 mean_circ: 0.934123
Ran for 84050 frames Cell Events: 4771 mean_circ: 0.934091
Ran for 85550 frames Cell Events: 4829 mean_circ: 0.934079
Ran for 86550 frames Cell Events: 4891 mean_circ: 0.934092
Ran for 87550 frames Cell Events: 4944 mean_circ: 0.934038
Ran for 89050 frames Cell Events: 5054 mean_circ: 0.934109
Ran for 89550 frames Cell Events: 5088 mean_circ: 0.934085
Ran for 90050 frames Cell Events: 5125 mean_circ: 0.934068
Ran for 91050 frames Cell Events: 5210 mean_circ: 0.93408
Ran for 91550 frames Cell Events: 5232 mean_circ: 0.93413
Ran for 93550 frames Cell Events: 5362 mean_circ: 0.934185
Ran for 94050 frames Cell Events: 5403 mean_circ: 0.934249
Ran for 94550 frames Cell Events: 5437 mean_circ: 0.934206
Ran for 95050 frames Cell Events: 5478 mean_circ: 0.934246
Ran for 96050 frames Cell Events: 5514 mean_circ: 0.934248
Ran for 96550 frames Cell Events: 5530 mean_circ: 0.934248
Ran for 97550 frames Cell Events: 5581 mean_circ: 0.934238
Ran for 98550 frames Cell Events: 5625 mean_circ: 0.934304
Ran for 100550 frames Cell Events: 5744 mean_circ: 0.934309
Ran for 101050 frames Cell Events: 5772 mean_circ: 0.934344
Ran for 102050 frames Cell Events: 5831 mean_circ: 0.934318
Ran for 102550 frames Cell Events: 5853 mean_circ: 0.934252
Ran for 103050 frames Cell Events: 5879 mean_circ: 0.934262
Ran for 104050 frames Cell Events: 5939 mean_circ: 0.934135
Ran for 105050 frames Cell Events: 5989 mean_circ: 0.934064
Ran for 106550 frames Cell Events: 6097 mean_circ: 0.934071
Ran for 107050 frames Cell Events: 6143 mean_circ: 0.934073
Ran for 110050 frames Cell Events: 6347 mean_circ: 0.934181
Ran for 110550 frames Cell Events: 6372 mean_circ: 0.934197
Ran for 111550 frames Cell Events: 6435 mean_circ: 0.934265
Ran for 112050 frames Cell Events: 6457 mean_circ: 0.934296
Ran for 112550 frames Cell Events: 6478 mean_circ: 0.934349
Ran for 113050 frames Cell Events: 6506 mean_circ: 0.934401
Ran for 113550 frames Cell Events: 6536 mean_circ: 0.934379
Ran for 114050 frames Cell Events: 6569 mean_circ: 0.934416
Ran for 114550 frames Cell Events: 6594 mean_circ: 0.934277
Ran for 118550 frames Cell Events: 6816 mean_circ: 0.934331
Ran for 119050 frames Cell Events: 6848 mean_circ: 0.93434
Ran for 119550 frames Cell Events: 6871 mean_circ: 0.934345
Ran for 120050 frames Cell Events: 6885 mean_circ: 0.934325
Ran for 120550 frames Cell Events: 6919 mean_circ: 0.934347
Ran for 121550 frames Cell Events: 6980 mean_circ: 0.93434
Ran for 122050 frames Cell Events: 7008 mean_circ: 0.93434
Ran for 122550 frames Cell Events: 7039 mean_circ: 0.934305
Ran for 125050 frames Cell Events: 7170 mean_circ: 0.934257
Ran for 125550 frames Cell Events: 7193 mean_circ: 0.934204
Ran for 127050 frames Cell Events: 7258 mean_circ: 0.934188
Ran for 127550 frames Cell Events: 7282 mean_circ: 0.934137
Ran for 128550 frames Cell Events: 7338 mean_circ: 0.934131
Ran for 129050 frames Cell Events: 7370 mean_circ: 0.934129
Ran for 129550 frames Cell Events: 7397 mean_circ: 0.934123
Ran for 132050 frames Cell Events: 7564 mean_circ: 0.934069
Ran for 132550 frames Cell Events: 7590 mean_circ: 0.934061
Ran for 133550 frames Cell Events: 7642 mean_circ: 0.933942
Ran for 134050 frames Cell Events: 7671 mean_circ: 0.933928
Ran for 134550 frames Cell Events: 7695 mean_circ: 0.933923
Ran for 135050 frames Cell Events: 7722 mean_circ: 0.933911
Ran for 135550 frames Cell Events: 7750 mean_circ: 0.933909
Ran for 136050 frames Cell Events: 7779 mean_circ: 0.933938
Ran for 136550 frames Cell Events: 7814 mean_circ: 0.933933
Ran for 138050 frames Cell Events: 7922 mean_circ: 0.933899
Ran for 139550 frames Cell Events: 8024 mean_circ: 0.933917



End this measurment..



Stopping time: 1.84722e+009
****************************************
Measurement finished

Mean Circularitiy: 0.933922
Mean Area: 598.52
Mean FL-1 intensity: 3473.88
Mean FL-2 intensity: 4425.7
Mean FL-3 intensity: 0

frames processed: 137331
Total Time: 69997ms
Number of Runs (new bg img) 0
Frames collected: 139838
Skipped frames: 31408
Rescued Frames: 1395598
Samples DAQ: 34959778
Samples written: 69918894
Samples per Channel: 34959447
FPS: 1997.77

Samples per second: 499442
Expected samples/s: 500000

Events: 8036
Events per second: 114.805
jumped back (PMT): 17
#Frames read, frequency
# 0 frames :205105
# 1 frames :62645
# 2 frames :2542
# 3 frames :1050
# 4 frames :353
# 5 frames :2602
# 6 frames :1566
# 7 frames :889
# 8 frames :505
# 9 frames :339
# 10 frames :377
# 11 frames :320
# 12 frames :257
# 13 frames :176
# 14 frames :161
# 15 frames :112
# 16 frames :98
# 17 frames :96
# 18 frames :72
# 19 frames :61
# 20 frames :59
# 21 frames :45
# 22 frames :36
# 23 frames :22
# 24 frames :14
# 25 frames :15
# 26 frames :7
# 27 frames :20
# 28 frames :9
# 29 frames :9
# 30 frames :9
# 31 frames :7
# 32 frames :7
# 33 frames :2
# 34 frames :6
# 35 frames :2
# 36 frames :1
# 37 frames :2
# 38 frames :5
# 39 frames :1
# 41 frames :3
# 44 frames :1
# 45 frames :1
# 48 frames :1
# 50 frames :1
# 51 frames :1
# 52 frames :1
# 54 frames :1
# 63 frames :1
# 67 frames :1
# 82 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.5901
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0