Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_13.25.21 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "b324e369575366382db236b6fd7301dd",
"name": "M1_20170605_1804_dsRNA_K2_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "11:25:41"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8050 (max 8051) in '8050'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.6947
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.84926e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 52 mean_circ: 0.930015
Ran for 2550 frames Cell Events: 124 mean_circ: 0.935795
Ran for 3050 frames Cell Events: 150 mean_circ: 0.936184
Ran for 3550 frames Cell Events: 190 mean_circ: 0.936824
Ran for 4050 frames Cell Events: 213 mean_circ: 0.937834
Ran for 6550 frames Cell Events: 378 mean_circ: 0.93842
Ran for 8550 frames Cell Events: 500 mean_circ: 0.939147
Ran for 9050 frames Cell Events: 518 mean_circ: 0.939452
Ran for 11050 frames Cell Events: 635 mean_circ: 0.939688
Ran for 12050 frames Cell Events: 683 mean_circ: 0.939711
Ran for 13050 frames Cell Events: 737 mean_circ: 0.93937
Ran for 13550 frames Cell Events: 762 mean_circ: 0.93968
Ran for 14050 frames Cell Events: 781 mean_circ: 0.939661
Ran for 15550 frames Cell Events: 874 mean_circ: 0.93946
Ran for 16050 frames Cell Events: 896 mean_circ: 0.939644
Ran for 17050 frames Cell Events: 929 mean_circ: 0.939968
Ran for 17550 frames Cell Events: 946 mean_circ: 0.93981
Ran for 18050 frames Cell Events: 996 mean_circ: 0.939741
Ran for 18550 frames Cell Events: 1028 mean_circ: 0.939502
Ran for 19550 frames Cell Events: 1108 mean_circ: 0.938507
Ran for 20050 frames Cell Events: 1132 mean_circ: 0.938461
Ran for 20550 frames Cell Events: 1166 mean_circ: 0.938226
Ran for 21050 frames Cell Events: 1202 mean_circ: 0.938233
Ran for 21550 frames Cell Events: 1229 mean_circ: 0.938418
Ran for 22550 frames Cell Events: 1298 mean_circ: 0.938768
Ran for 23550 frames Cell Events: 1359 mean_circ: 0.938668
Ran for 24050 frames Cell Events: 1385 mean_circ: 0.938934
Ran for 24550 frames Cell Events: 1422 mean_circ: 0.939012
Ran for 25050 frames Cell Events: 1454 mean_circ: 0.939003
Ran for 25550 frames Cell Events: 1473 mean_circ: 0.939002
Ran for 26050 frames Cell Events: 1497 mean_circ: 0.939064
Ran for 27050 frames Cell Events: 1562 mean_circ: 0.939277
Ran for 30050 frames Cell Events: 1791 mean_circ: 0.938792
Ran for 31550 frames Cell Events: 1880 mean_circ: 0.939234
Ran for 32050 frames Cell Events: 1918 mean_circ: 0.939285
Ran for 33050 frames Cell Events: 1968 mean_circ: 0.939268
Ran for 33550 frames Cell Events: 2003 mean_circ: 0.939313
Ran for 37550 frames Cell Events: 2282 mean_circ: 0.939497
Ran for 39550 frames Cell Events: 2407 mean_circ: 0.939434
Ran for 40050 frames Cell Events: 2442 mean_circ: 0.939496
Ran for 40550 frames Cell Events: 2464 mean_circ: 0.93955
Ran for 41050 frames Cell Events: 2489 mean_circ: 0.939704
Ran for 41550 frames Cell Events: 2509 mean_circ: 0.939719
Ran for 42550 frames Cell Events: 2568 mean_circ: 0.939841
Ran for 43050 frames Cell Events: 2606 mean_circ: 0.939855
Ran for 43550 frames Cell Events: 2642 mean_circ: 0.939838
Ran for 44550 frames Cell Events: 2700 mean_circ: 0.939876
Ran for 45050 frames Cell Events: 2727 mean_circ: 0.939911
Ran for 46050 frames Cell Events: 2782 mean_circ: 0.939878
Ran for 46550 frames Cell Events: 2812 mean_circ: 0.939883
Ran for 47050 frames Cell Events: 2848 mean_circ: 0.939887
Ran for 48050 frames Cell Events: 2903 mean_circ: 0.939972
Ran for 48550 frames Cell Events: 2940 mean_circ: 0.940086
Ran for 49050 frames Cell Events: 2976 mean_circ: 0.940054
Ran for 50050 frames Cell Events: 3059 mean_circ: 0.940079
Ran for 50550 frames Cell Events: 3091 mean_circ: 0.940076
Ran for 51550 frames Cell Events: 3177 mean_circ: 0.940115
Ran for 52050 frames Cell Events: 3208 mean_circ: 0.940123
Ran for 52550 frames Cell Events: 3242 mean_circ: 0.940068
Ran for 53050 frames Cell Events: 3268 mean_circ: 0.940032
Ran for 53550 frames Cell Events: 3306 mean_circ: 0.939971
Ran for 54550 frames Cell Events: 3367 mean_circ: 0.939982
Ran for 55050 frames Cell Events: 3393 mean_circ: 0.939976
Ran for 56050 frames Cell Events: 3435 mean_circ: 0.939852
Ran for 57050 frames Cell Events: 3499 mean_circ: 0.939756
Ran for 59050 frames Cell Events: 3683 mean_circ: 0.939932
Ran for 59550 frames Cell Events: 3708 mean_circ: 0.939981
Ran for 60050 frames Cell Events: 3734 mean_circ: 0.939931
Ran for 60550 frames Cell Events: 3760 mean_circ: 0.939978
Ran for 61050 frames Cell Events: 3796 mean_circ: 0.94
Ran for 62050 frames Cell Events: 3853 mean_circ: 0.939861
Ran for 62550 frames Cell Events: 3878 mean_circ: 0.939794
Ran for 63050 frames Cell Events: 3902 mean_circ: 0.939815
Ran for 64550 frames Cell Events: 4012 mean_circ: 0.939858
Ran for 65050 frames Cell Events: 4039 mean_circ: 0.939862
Ran for 66050 frames Cell Events: 4126 mean_circ: 0.939697
Ran for 66550 frames Cell Events: 4155 mean_circ: 0.939709
Ran for 67050 frames Cell Events: 4182 mean_circ: 0.939713
Ran for 67550 frames Cell Events: 4202 mean_circ: 0.939728
Ran for 68050 frames Cell Events: 4233 mean_circ: 0.939769
Ran for 69550 frames Cell Events: 4323 mean_circ: 0.939725
Ran for 70550 frames Cell Events: 4379 mean_circ: 0.93963
Ran for 71050 frames Cell Events: 4405 mean_circ: 0.939605
Ran for 72050 frames Cell Events: 4468 mean_circ: 0.939682
Ran for 72550 frames Cell Events: 4498 mean_circ: 0.939574
Ran for 73050 frames Cell Events: 4532 mean_circ: 0.939502
Ran for 74050 frames Cell Events: 4579 mean_circ: 0.939569
Ran for 74550 frames Cell Events: 4603 mean_circ: 0.93962
Ran for 75050 frames Cell Events: 4636 mean_circ: 0.93963
Ran for 75550 frames Cell Events: 4671 mean_circ: 0.939584
Ran for 76550 frames Cell Events: 4731 mean_circ: 0.939549
Ran for 77050 frames Cell Events: 4760 mean_circ: 0.939529
Ran for 79550 frames Cell Events: 4948 mean_circ: 0.939462
Ran for 80050 frames Cell Events: 4988 mean_circ: 0.939461
Ran for 81050 frames Cell Events: 5080 mean_circ: 0.939471
Ran for 81550 frames Cell Events: 5104 mean_circ: 0.939413
Ran for 82050 frames Cell Events: 5130 mean_circ: 0.939443
Ran for 83550 frames Cell Events: 5228 mean_circ: 0.939391
Ran for 84050 frames Cell Events: 5245 mean_circ: 0.939382
Ran for 85050 frames Cell Events: 5309 mean_circ: 0.939336
Ran for 85550 frames Cell Events: 5348 mean_circ: 0.939348
Ran for 86550 frames Cell Events: 5422 mean_circ: 0.939255
Ran for 88550 frames Cell Events: 5535 mean_circ: 0.939258
Ran for 89050 frames Cell Events: 5558 mean_circ: 0.939261
Ran for 91550 frames Cell Events: 5707 mean_circ: 0.939206
Ran for 93550 frames Cell Events: 5833 mean_circ: 0.938978
Ran for 94050 frames Cell Events: 5883 mean_circ: 0.938978
Ran for 96050 frames Cell Events: 6032 mean_circ: 0.938954
Ran for 97550 frames Cell Events: 6122 mean_circ: 0.938869
Ran for 98050 frames Cell Events: 6153 mean_circ: 0.938875
Ran for 98550 frames Cell Events: 6195 mean_circ: 0.938947
Ran for 100550 frames Cell Events: 6335 mean_circ: 0.938831
Ran for 101550 frames Cell Events: 6395 mean_circ: 0.938879
Ran for 102550 frames Cell Events: 6471 mean_circ: 0.938856
Ran for 103050 frames Cell Events: 6507 mean_circ: 0.93891
Ran for 104050 frames Cell Events: 6570 mean_circ: 0.938947
Ran for 104550 frames Cell Events: 6600 mean_circ: 0.938968
Ran for 105050 frames Cell Events: 6635 mean_circ: 0.938924
Ran for 105550 frames Cell Events: 6671 mean_circ: 0.938961
Ran for 107050 frames Cell Events: 6802 mean_circ: 0.938866
Ran for 107550 frames Cell Events: 6836 mean_circ: 0.938882
Ran for 109050 frames Cell Events: 6941 mean_circ: 0.938918
Ran for 109550 frames Cell Events: 6965 mean_circ: 0.938953
Ran for 111050 frames Cell Events: 7084 mean_circ: 0.938931
Ran for 111550 frames Cell Events: 7107 mean_circ: 0.938933
Ran for 113050 frames Cell Events: 7203 mean_circ: 0.938974
Ran for 113550 frames Cell Events: 7243 mean_circ: 0.939006
Ran for 114050 frames Cell Events: 7288 mean_circ: 0.938965
Ran for 114550 frames Cell Events: 7314 mean_circ: 0.938967
Ran for 115050 frames Cell Events: 7340 mean_circ: 0.938959
Ran for 115550 frames Cell Events: 7364 mean_circ: 0.938984
Ran for 116050 frames Cell Events: 7406 mean_circ: 0.939006
Ran for 116550 frames Cell Events: 7433 mean_circ: 0.938988
Ran for 118550 frames Cell Events: 7579 mean_circ: 0.939028
Ran for 119550 frames Cell Events: 7652 mean_circ: 0.939018
Ran for 120050 frames Cell Events: 7680 mean_circ: 0.938973
Ran for 120550 frames Cell Events: 7711 mean_circ: 0.938956
Ran for 121050 frames Cell Events: 7741 mean_circ: 0.938949
Ran for 122050 frames Cell Events: 7805 mean_circ: 0.938964
Ran for 122550 frames Cell Events: 7835 mean_circ: 0.938922
Ran for 123050 frames Cell Events: 7862 mean_circ: 0.938929
Ran for 125050 frames Cell Events: 8020 mean_circ: 0.938991



End this measurment..



Stopping time: 1.84932e+009
****************************************
Measurement finished

Mean Circularitiy: 0.939004
Mean Area: 598.315
Mean FL-1 intensity: 3321.93
Mean FL-2 intensity: 4568.99
Mean FL-3 intensity: 0

frames processed: 125020
Total Time: 62877.2ms
Number of Runs (new bg img) 0
Frames collected: 125579
Skipped frames: 37630
Rescued Frames: 1912256
Samples DAQ: 31395619
Samples written: 62789346
Samples per Channel: 31394673
FPS: 1997.21

Samples per second: 499302
Expected samples/s: 500000

Events: 8051
Events per second: 128.043
jumped back (PMT): 7
#Frames read, frequency
# 0 frames :189798
# 1 frames :49705
# 2 frames :2490
# 3 frames :1135
# 4 frames :380
# 5 frames :2134
# 6 frames :1238
# 7 frames :1020
# 8 frames :497
# 9 frames :360
# 10 frames :592
# 11 frames :323
# 12 frames :336
# 13 frames :210
# 14 frames :166
# 15 frames :136
# 16 frames :102
# 17 frames :98
# 18 frames :79
# 19 frames :49
# 20 frames :50
# 21 frames :39
# 22 frames :29
# 23 frames :22
# 24 frames :29
# 25 frames :19
# 26 frames :10
# 27 frames :12
# 28 frames :8
# 29 frames :8
# 30 frames :5
# 31 frames :10
# 32 frames :4
# 33 frames :4
# 34 frames :2
# 35 frames :3
# 36 frames :2
# 37 frames :2
# 38 frames :4
# 39 frames :2
# 41 frames :1
# 42 frames :2
# 44 frames :1
# 45 frames :1
# 49 frames :1
# 54 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.6947
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0